Status
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Gene information
General information
TC013210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12279798..12284150
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013210
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09113

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013210 RA
      MRNVFDSKE LGGQTLQLA EMLMDCAAV VIASGDGLP 
      NLLCITCED KLNLAYEFK QQCQKSDTT LRELTNQSD 
      SVIKEETCD IVVQPDFNI PELYNDTDS EDDDSKPKS 
      FTCPFCQKV LRTKKGLRI HQRRHTGDK LRSCHLCNA 
      QFTRTHHLI RHMKTHVKG GDTGHVCGE CGMSFMKLS 
      HLLSHKKTH KGDKSDEGE SGQQSTAAD NNSDCDNDI 
      TLEINEDED DDDDDALEE YFKPKTKTS KKDKGTYEC 
      KFCFKIMTT FVGLKIHMR RHTGSDLAK CKLCDKSFT 
      KTSHLKRHL QTHGIKELD KPKPAEKKV MECEFCDRK 
      FKYKKSFNH HMQTEHGMS DESDVPLSA YISKMKEPE 
      ESKEDSSEK PEKTDEAEP SSDQDTKEE IPPVAKPVH 
      HKVHVCHVC EAAFARANH LTRHMTLHR ALLIHKCDR 
      CDKAYATLE HLAKHVEED HINKPYACT ICNKPFSRG 
      EHLIRHLKV HQTGNEKED NLTCSICEK TFSRSDHLA 
      RHIKIHLLQ DKRHVCSEC GKAFNRLDN LKTHQRIHT 
      GIKDTTKLH LCIYCGKEF NNSSNMIVH MRRHTGERP 
      YKCSQCGKG FPRSHDLKC HERTHSGEK PYHCALCGK 
      SFNKSNKLL RHTRVHTGE RPYVCNICG RAFTQSNDL 
      ALHMRRHTG ARPYACGMC PARFIQSGQ LKTHRRTTG 
      HWVETQPDL KGGHRVEPV TPVMEPTPI RFKTHGKKK 
      DDEVDEAKG KILAHTMGL MGPLNVSGE NLVIDPKIV 
      ELQNSGLIN LMGAGEVKF KEGGFVVSQ VKSEGEKVQ 
      GFGQGGAVT YSIVAPVAS STTFTAADY SNYQNFG
    CDS sequence
    • >TC013210 RA
      atgcgaaat gtgttcgat tccaaagag ctcggcggc 
      caaacttta caactcgct gagatgcta atggactgc 
      gctgccgtt gttattgcc tcaggggac ggccttccc 
      aaccttttg tgcataacc tgcgaagac aagctcaac 
      ctcgcctac gagttcaag cagcagtgc cagaagtcc 
      gacacgact ctgcgggag ctcaccaac cagtcagac 
      tccgtgatc aaggaggag acctgcgat atagtcgtc 
      cagcctgac ttcaacatc ccagagctt tacaatgac 
      acggacagc gaagatgac gactcgaag cccaagtct 
      ttcacgtgc cccttctgc cagaaggtg ctaaggacg 
      aagaaggga ctccgcatc caccagcgg cgccacacg 
      ggggacaaa ctgcgctcc tgccatttg tgcaacgct 
      cagttcacg aggactcat cacctgatc aggcacatg 
      aagacgcac gtgaagggg ggcgacacg gggcacgtg 
      tgcggcgaa tgcggaatg tctttcatg aaactgagt 
      cacttgctc tcgcacaag aagactcat aagggggat 
      aagagcgat gagggcgaa agcgggcag cagtccacg 
      gctgcggat aataacagt gattgcgat aatgatata 
      acgttggag atcaacgag gatgaggac gacgacgat 
      gatgatgct ttggaggag tattttaag ccaaagacg 
      aaaacgtcg aaaaaagat aaagggact tatgaatgt 
      aaattttgt tttaagatt atgacgacg tttgtcggg 
      cttaagata cacatgagg aggcatacg gggtcagac 
      ttggctaag tgtaaatta tgtgacaaa agctttacg 
      aaaaccagc cacctgaag cgccacctc caaacccac 
      ggcatcaaa gagctcgac aagccgaaa cccgccgaa 
      aagaaagta atggaatgc gaattctgc gaccgaaaa 
      ttcaaatac aagaaaagt ttcaaccac cacatgcaa 
      accgagcac gggatgtcg gacgaaagc gacgttcct 
      ctcagtgct tacatttca aaaatgaaa gagcccgaa 
      gaatccaaa gaggattcg agcgaaaag cccgaaaag 
      acggacgag gcggagccg agttcggat caagacacg 
      aaagaggaa attccgccg gtggcgaaa cccgtccac 
      cacaaggtc cacgtgtgt cacgtgtgc gaggcagcg 
      ttcgcacga gccaatcat ttgacacga cacatgacc 
      ttgcacagg gcactcttg atacacaag tgcgacagg 
      tgcgacaag gcctatgcg accttggag catttggcc 
      aaacatgtg gaggaggac catattaat aagccgtat 
      gcgtgtacg atttgtaat aaaccgttc agtagaggg 
      gaacatttg ataagacat ttgaaggtg catcaaacc 
      gggaacgag aaagaggat aatttgacg tgctcaata 
      tgcgaaaag actttcagc aggtcggat catttagcg 
      cgccatatt aaaatacat ttgttgcaa gacaagaga 
      catgtttgt tccgagtgt ggtaaagcg tttaatcga 
      ctcgataat ctcaagact catcagagg atccatacg 
      ggtatcaaa gatactacg aaattgcat ctttgtatt 
      tattgcggc aaggagttt aataattcg agcaatatg 
      attgttcat atgagacga catacgggg gaaagaccg 
      tataaatgc agtcagtgt ggtaagggc tttcccaga 
      tcgcatgat ctcaagtgt catgaaagg acacactca 
      ggggaaaaa ccctaccat tgcgcgttg tgtgggaaa 
      tcattcaat aagagcaat aagttgtta cgccacacg 
      cgcgtccac accggcgag cgcccctac gtgtgcaac 
      atttgcgga cgggccttc acccagtcc aacgacttg 
      gcgctccac atgcgccgg cacacgggg gcgcggccc 
      tacgcctgt ggcatgtgt ccggcgcgt tttatccaa 
      tccggacag ttgaaaacc caccgacgt accaccggc 
      cattgggtg gagacccag ccggacctg aaagggggc 
      caccgggtc gagcctgtc acccccgtg atggagccc 
      acccccatt cggttcaaa acccacggc aagaaaaag 
      gacgacgag gtggacgaa gccaagggc aaaattctg 
      gcccatacg atgggcctg atggggccg ttgaacgtc 
      tcaggggag aatctggtg attgacccg aaaattgtc 
      gaattgcaa aattcgggt ttgatcaat ttgatgggt 
      gcaggggag gtgaagttc aaagagggc gggtttgtg 
      gtgtcgcag gtgaagagt gagggggag aaggttcaa 
      gggtttggg caagggggc gccgtgacg tactcgata 
      gtggcgccc gtggcgagt tcgacgact ttcacggcc 
      gcggattat agcaattat cagaatttc ggttga
    mRNA sequence
    • >TC013210 RA
      aagtgctcc aaaatttcc tcaatttta aaaattcca 
      ggaaatttc tatttctgt gagctactt gcgatttcc 
      tagacctcg acatgattc caaaacaat tattttctc 
      attcaacaa acactggga ttctaacgt cagcggcac 
      gttagtcac ctcctcttg aacgtcgtt tgaggttat 
      ttttctttc agatttttt tgagttaaa ttgcaataa 
      acaaaaata aattagaat gagtgagaa aaaagcccc 
      ggcgcctgg cgaaggcct tagatctct cagaagtac 
      aacattgtt tagagctgt gaattttga attatacgt 
      aaaaccaaa tttggtaat aatgggatt aggactagt 
      ggcttttac ggggtgttt gggctatat tgcatatat 
      gaggaacaa gtatgacgc acttgggta ttaccctgc 
      aattcagga ggacggacg agaggaata tgtgaaacg 
      gaagtcgaa atgggacga ttgagtagt tattagttc 
      caaaatagt ttttcttgt aaatagtaa atgattcaa 
      ccacaggat tcaaggttg gcgccttga cagctgtac 
      caatcgtca aacttgtgt cacttttaa attattaat 
      ttattgttt aatcacttg tataaataa attcatatt 
      gttgtagac gagtgttta ccttcccgt tttcgtgcg 
      tttttaacc tcgtgtggg aaaacgcca gagtaatga 
      taaattcat tgcgcaaaa tcgcggatc gcgtcctgt 
      gcttacccc aaccaacaa caaatagtg cctgaaata 
      gtttcggca attatgcaa aaaactgtt attatttaa 
      caagatcgg gtgttttta taatgatta ataaaacca 
      cggagttag ttatcgata aaatctgcc gttcatgta 
      tgtgccaaa atgagaata tgcgaaatg tgttcgatt 
      ccaaagagc tcggcggcc aaactttac aactcgctg 
      agatgctaa tggactgcg ctgccgttg ttattgcct 
      caggggacg gccttccca accttttgt gcataacct 
      gcgaagaca agctcaacc tcgcctacg agttcaagc 
      agcagtgcc agaagtccg acacgactc tgcgggagc 
      tcaccaacc agtcagact ccgtgatca aggaggaga 
      cctgcgata tagtcgtcc agcctgact tcaacatcc 
      cagagcttt acaatgaca cggacagcg aagatgacg 
      actcgaagc ccaagtctt tcacgtgcc ccttctgcc 
      agaaggtgc taaggacga agaagggac tccgcatcc 
      accagcggc gccacacgg gggacaaac tgcgctcct 
      gccatttgt gcaacgctc agttcacga ggactcatc 
      acctgatca ggcacatga agacgcacg tgaaggggg 
      gcgacacgg ggcacgtgt gcggcgaat gcggaatgt 
      ctttcatga aactgagtc acttgctct cgcacaaga 
      agactcata agggggata agagcgatg agggcgaaa 
      gcgggcagc agtccacgg ctgcggata ataacagtg 
      attgcgata atgatataa cgttggaga tcaacgagg 
      atgaggacg acgacgatg atgatgctt tggaggagt 
      attttaagc caaagacga aaacgtcga aaaaagata 
      aagggactt atgaatgta aattttgtt ttaagatta 
      tgacgacgt ttgtcgggc ttaagatac acatgagga 
      ggcatacgg ggtcagact tggctaagt gtaaattat 
      gtgacaaaa gctttacga aaaccagcc acctgaagc 
      gccacctcc aaacccacg gcatcaaag agctcgaca 
      agccgaaac ccgccgaaa agaaagtaa tggaatgcg 
      aattctgcg accgaaaat tcaaataca agaaaagtt 
      tcaaccacc acatgcaaa ccgagcacg ggatgtcgg 
      acgaaagcg acgttcctc tcagtgctt acatttcaa 
      aaatgaaag agcccgaag aatccaaag aggattcga 
      gcgaaaagc ccgaaaaga cggacgagg cggagccga 
      gttcggatc aagacacga aagaggaaa ttccgccgg 
      tggcgaaac ccgtccacc acaaggtcc acgtgtgtc 
      acgtgtgcg aggcagcgt tcgcacgag ccaatcatt 
      tgacacgac acatgacct tgcacaggg cactcttga 
      tacacaagt gcgacaggt gcgacaagg cctatgcga 
      ccttggagc atttggcca aacatgtgg aggaggacc 
      atattaata agccgtatg cgtgtacga tttgtaata 
      aaccgttca gtagagggg aacatttga taagacatt 
      tgaaggtgc atcaaaccg ggaacgaga aagaggata 
      atttgacgt gctcaatat gcgaaaaga ctttcagca 
      ggtcggatc atttagcgc gccatatta aaatacatt 
      tgttgcaag acaagagac atgtttgtt ccgagtgtg 
      gtaaagcgt ttaatcgac tcgataatc tcaagactc 
      atcagagga tccatacgg gtatcaaag atactacga 
      aattgcatc tttgtattt attgcggca aggagttta 
      ataattcga gcaatatga ttgttcata tgagacgac 
      atacggggg aaagaccgt ataaatgca gtcagtgtg 
      gtaagggct ttcccagat cgcatgatc tcaagtgtc 
      atgaaagga cacactcag gggaaaaac cctaccatt 
      gcgcgttgt gtgggaaat cattcaata agagcaata 
      agttgttac gccacacgc gcgtccaca ccggcgagc 
      gcccctacg tgtgcaaca tttgcggac gggccttca 
      cccagtcca acgacttgg cgctccaca tgcgccggc 
      acacggggg cgcggccct acgcctgtg gcatgtgtc 
      cggcgcgtt ttatccaat ccggacagt tgaaaaccc 
      accgacgta ccaccggcc attgggtgg agacccagc 
      cggacctga aagggggcc accgggtcg agcctgtca 
      cccccgtga tggagccca cccccattc ggttcaaaa 
      cccacggca agaaaaagg acgacgagg tggacgaag 
      ccaagggca aaattctgg cccatacga tgggcctga 
      tggggccgt tgaacgtct caggggaga atctggtga 
      ttgacccga aaattgtcg aattgcaaa attcgggtt 
      tgatcaatt tgatgggtg caggggagg tgaagttca 
      aagagggcg ggtttgtgg tgtcgcagg tgaagagtg 
      agggggaga aggttcaag ggtttgggc aagggggcg 
      ccgtgacgt actcgatag tggcgcccg tggcgagtt 
      cgacgactt tcacggccg cggattata gcaattatc 
      agaatttcg gttgaatag gagagtaat ttaagtgat 
      aacttagtt attgtgtgt ttgagtaag aagtgtctg 
      aaaaataag agggcgaca cgcgtgact gatgtgcca 
      gtttgaagt acgagtagt ttcgaacta cagcgccag 
      ttcggattc agttttcat agtctgatc aatgtatat 
      ttgtcagtt cgtgtttac tattaagaa tctatgaat 
      aaacttaat atattctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY