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Gene information
General information
TC014503 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11288111..11291486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014503
    iBeetle screen iB_09106

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014503 RA
      MAFLCPVRI RRGKKKKST ISDLDKDLN RPINGAPGM 
      GRITGSASI ETLVRVGIE KEHGLSPDS KMVVLHDFT 
      PCVDDELEV KRGQIVNIL YRENDWVYV IGLDTRQEG 
      FIPYSYCSP YNSHLAELA IKKKLPRET QLPSGESSD 
      TNHECEGGL DTSDCDSLG KNKVRGAGS PISIRSEPD 
      MLPFSKDPS GRYIVLYTF IARDENDVS VERGEFVTV 
      LNKEDPDWY WIVRSDGQE GFIPSGFVY PADNVIQGH 
      LNRVTNQNL GGSLPNQNN SHLLTSSSV DDLRYHGTE 
      LVMLYDYKA QAPDDLTVR RGDWIYADL NNQTVDGWL 
      WAYAPKTRK YGFIPKAYA RPPAMTSL
    CDS sequence
    • >TC014503 RA
      atggcattt ctttgccct gttcgtatc agacgcggc 
      aagaagaaa aaatctaca atctccgac ctcgacaaa 
      gacctcaac cggcccatc aatggggcc cccggcatg 
      ggccgaatc accggctcg gccagcatc gagaccctc 
      gtccgtgtc ggaatcgaa aaagagcac gggctttcc 
      cccgattcg aaaatggtg gttctccac gacttcacc 
      ccttgcgtg gacgacgaa ctcgaagtg aaacgtgga 
      cagatcgtc aacatcctc taccgggag aacgactgg 
      gtttatgtg atcggttta gacaccaga caggagggt 
      ttcatccct tattcgtac tgttcgcca tacaacagc 
      catttggcc gaactggcc atcaagaag aaactccca 
      agggagacg caactccca tcgggggag agctcggac 
      acgaaccat gaatgtgaa gggggtttg gatacgagt 
      gactgcgac tcgttgggg aaaaataaa gttcgaggg 
      gctgggagt cctattagt atccggtcc gaaccggat 
      atgttgcca ttttcgaag gatccgtcc gggagatat 
      attgttttg tacacgttt atagccagg gatgagaat 
      gatgtgtcg gtcgagcgt ggcgagttt gttactgtt 
      ttgaacaag gaggacccg gattggtac tggatcgtg 
      cgcagcgac gggcaggag ggttttatc ccgtcaggg 
      tttgtctac ccggccgat aacgtgatt cagggccat 
      ttgaatcga gtcacgaat cagaatctg ggtggcagt 
      ttgccaaac cagaacaat tcgcatcta ttgacatcg 
      tcaagtgtt gacgatttg cgttatcac ggaacggaa 
      ttggtgatg ttgtacgat tataaagcg caagctcct 
      gatgattta actgtaaga agaggtgat tggatttat 
      gccgacttg aataatcaa actgtggat gggtggttg 
      tgggcgtat gcgcccaag acgaggaaa tatgggttc 
      atacccaag gcttacgca aggccgcct gctatgacg 
      agtctttga
    mRNA sequence
    • >TC014503 RA
      gtgatttga tcaaaaaga tcattgtgt ttgacagaa 
      gtacagttg taattattg gaatattaa gtgaattgt 
      ttgaaaatg agataaacg ataaatgaa aaatggcat 
      ttctttgcc ctgttcgta tcagacgcg gcaagaaga 
      aaaaatcta caatctccg acctcgaca aagacctca 
      accggccca tcaatgggg cccccggca tgggccgaa 
      tcaccggct cggccagca tcgagaccc tcgtccgtg 
      tcggaatcg aaaaagagc acgggcttt cccccgatt 
      cgaaaatgg tggttctcc acgacttca ccccttgcg 
      tggacgacg aactcgaag tgaaacgtg gacagatcg 
      tcaacatcc tctaccggg agaacgact gggtttatg 
      tgatcggtt tagacacca gacaggagg gtttcatcc 
      cttattcgt actgttcgc catacaaca gccatttgg 
      ccgaactgg ccatcaaga agaaactcc caagggaga 
      cgcaactcc catcggggg agagctcgg acacgaacc 
      atgaatgtg aagggggtt tggatacga gtgactgcg 
      actcgttgg ggaaaaata aagttcgag gggctggga 
      gtcctatta gtatccggt ccgaaccgg atatgttgc 
      cattttcga aggatccgt ccgggagat atattgttt 
      tgtacacgt ttatagcca gggatgaga atgatgtgt 
      cggtcgagc gtggcgagt ttgttactg ttttgaaca 
      aggaggacc cggattggt actggatcg tgcgcagcg 
      acgggcagg agggtttta tcccgtcag ggtttgtct 
      acccggccg ataacgtga ttcagggcc atttgaatc 
      gagtcacga atcagaatc tgggtggca gtttgccaa 
      accagaaca attcgcatc tattgacat cgtcaagtg 
      ttgacgatt tgcgttatc acggaacgg aattggtga 
      tgttgtacg attataaag cgcaagctc ctgatgatt 
      taactgtaa gaagaggtg attggattt atgccgact 
      tgaataatc aaactgtgg atgggtggt tgtgggcgt 
      atgcgccca agacgagga aatatgggt tcataccca 
      aggcttacg caaggccgc ctgctatga cgagtcttt 
      gattgtgtg aatggattt ttaataaga cattttata 
      tttttatac atttttatt gccagacat ttattgtta 
      ataaacttt tacataaga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY