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Gene information
General information
TC014485 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11073560..11074924
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014485
    iBeetle screen iB_09104

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014485 RA
      MEQLICRTC LKTNLSSTN CVQIYNLVE TNQMTIKDM 
      LELFMPEMD LDLFEGEVV MCVQCSDSL RRAYLFKDT 
      CLKTEERIY DYLQQYDIK LGKKISLRN IITHNLAGS 
      KDLGDFKVP EGYLTSVQD SSYLNNTVI KPENPELGK 
      SRKRSHSSG TKKSKSDNS SLKRSYTCD LCEYKAYRL 
      DLLNTHKQK HDKNMLSVK EYKCDFCTY TTLRKRALD 
      RHLFTHKGK SPHLCQICN KPFAEKYQL KLHMITHTG 
      KELPHKCDE CGKGYLTKS YLKQHKETR HLPEKCTEC 
      DDDECGHAT RCYKCNLCE ASFTDRRGL EAHTFTHTG 
      GELPYKCPH CSFSTAWKG NLKKHLNNK H
    CDS sequence
    • >TC014485 RA
      atggagcag ttaatttgt agaacttgc ctcaaaacc 
      aatctttcc tccacgaat tgtgtccag atttacaat 
      ttggtcgag acaaatcaa atgacgatc aaagacatg 
      ttggagtta tttatgccc gaaatggac ctggactta 
      ttcgagggt gaagtagtg atgtgcgtt caatgttcc 
      gactccttg agacgggct taccttttc aaagacact 
      tgcttgaag acagaggaa cgcatttac gattacttg 
      caacagtac gacattaaa ctgggcaaa aaaattagt 
      ttgagaaac attataacg cacaatctg gcgggaagc 
      aaggacttg ggcgatttt aaagtaccg gaaggttat 
      ttaacttcg gtgcaagac agcagttac ctaaacaac 
      acagtgata aaaccggaa aacccagag ttaggaaaa 
      tcacgtaag aggagtcat tcaagtggt acaaaaaaa 
      tctaaaagc gataacagt tcgttaaaa cgcagctac 
      acgtgcgac ttatgcgaa tacaaagcc tacagatta 
      gacttactt aacacccac aaacaaaaa cacgacaaa 
      aacatgttg agcgtgaaa gaatacaag tgtgatttt 
      tgcacctac accactttg cgtaaacgg gccttggac 
      cgacactta ttcactcac aagggcaaa tcgccacat 
      ttgtgccaa atctgcaac aaaccgttt gcggaaaaa 
      taccagtta aagttacac atgatcaca catacggga 
      aaagagctt ccacataag tgtgacgag tgtggcaaa 
      ggctatttg accaagtcg tatttaaaa cagcataag 
      gagacgaga catttgccc gaaaaatgc acagagtgt 
      gatgatgat gagtgtgga cacgcaact aggtgctat 
      aagtgtaat ttgtgcgag gctagtttt accgatagg 
      agagggctt gaggcgcat acttttaca cacacgggg 
      ggcgaattg ccctacaag tgcccccac tgtagtttc 
      agcacggcc tggaagggc aatttgaaa aaacacctc 
      aacaataag cattaa
    mRNA sequence
    • >TC014485 RA
      agttaataa aatggagca gttaatttg tagaacttg 
      cctcaaaac caatctttc ctccacgaa ttgtgtcca 
      gatttacaa tttggtcga gacaaatca aatgacgat 
      caaagacat gttggagtt atttatgcc cgaaatgga 
      cctggactt attcgaggg tgaagtagt gatgtgcgt 
      tcaatgttc cgactcctt gagacgggc ttacctttt 
      caaagacac ttgcttgaa gacagagga acgcattta 
      cgattactt gcaacagta cgacattaa actgggcaa 
      aaaaattag tttgagaaa cattataac gcacaatct 
      ggcgggaag caaggactt gggcgattt taaagtacc 
      ggaaggtta tttaacttc ggtgcaaga cagcagtta 
      cctaaacaa cacagtgat aaaaccgga aaacccaga 
      gttaggaaa atcacgtaa gaggagtca ttcaagtgg 
      tacaaaaaa atctaaaag cgataacag ttcgttaaa 
      acgcagcta cacgtgcga cttatgcga atacaaagc 
      ctacagatt agacttact taacaccca caaacaaaa 
      acacgacaa aaacatgtt gagcgtgaa agaatacaa 
      gtgtgattt ttgcaccta caccacttt gcgtaaacg 
      ggccttgga ccgacactt attcactca caagggcaa 
      atcgccaca tttgtgcca aatctgcaa caaaccgtt 
      tgcggaaaa ataccagtt aaagttaca catgatcac 
      acatacggg aaaagagct tccacataa gtgtgacga 
      gtgtggcaa aggctattt gaccaagtc gtatttaaa 
      acagcataa ggagacgag acatttgcc cgaaaaatg 
      cacagagtg tgatgatga tgagtgtgg acacgcaac 
      taggtgcta taagtgtaa tttgtgcga ggctagttt 
      taccgatag gagagggct tgaggcgca tacttttac 
      acacacggg gggcgaatt gccctacaa gtgccccca 
      ctgtagttt cagcacggc ctggaaggg caatttgaa 
      aaaacacct caacaataa gcattaaga aggggtttt 
      gtgacgcta cacggatag atggcagca ctgatattt 
      cgttgaaga gggccacgt ttttgcttt attggagta 
      ttatttatt tattttttc tgtcctgat tagcaatgt 
      cagaaagtt tcagaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY