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Gene information
General information
TC014456 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10602067..10604280
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014456
    iBeetle screen iB_09102

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014456 RA
      MEGRCRLCA EFGTDHLNS LDDLDLILR IFQLFQVRI 
      TSEDKLPTT ICQLCAETV KQTWEFNQH IQKSQEILQ 
      DIVNTELQA AHQESKLDF TLTLEESKL NVVEVTTSS 
      AVKTEDIVL KPSPKVKKT GRKKESDSE LSDVELNDD 
      GTVTPKAEL LGWHSYPWA CCDCQHTFT NSEDLREHH 
      ATVHGQAPT YPCADCPKI YTKYSTFLT HVRVHRVKL 
      RFCCDICAK WFPTVLAQK KHRNEHGDE RPHACTTCG 
      KRFRMQSAL MVHARSHLP AELKNKHQC DQCPKKFGT 
      KPNLMAHKR IHAGVRDYT CDQCGKSFV QKGNLDNHL 
      LTHTAARPF ACSTCGKAF KTLVRLRKH GSVHSGLKP 
      HQCDVCGRQ FRERGTLKE HHRIHTGAM PFTCEFCGK 
      SFRFKGVLT THRRQHTGE RPYSCHECQ HHFTNWPNY 
      NKHMKRRHG INTSVTVRK PQSIPPTGM PHRNPPGTV 
      LAPPQPITI TESFVETPA SFYPVLSLY NLPEELLQQ 
      RV
    CDS sequence
    • >TC014456 RA
      atggagggt cgctgccgt ctgtgcgcc gagttcggc 
      accgatcat ctaaacagt ctcgatgat ctcgatctc 
      attttacgc atttttcaa ctctttcaa gtccgaatc 
      acctccgag gacaaactc ccaacaaca atctgccaa 
      ttatgcgcc gaaacggtg aaacaaaca tgggagttc 
      aatcaacac atccagaaa tcgcaagag atcctccag 
      gacatcgtc aacacagaa ctgcaagca gcccaccag 
      gagagcaag ctcgatttc acgctcacg ctcgaagaa 
      agcaaactg aacgtggtc gaagtgacc acatcttca 
      gccgttaaa acggaagat atcgtactt aagccttca 
      ccaaaagtc aaaaaaacc ggccgtaaa aaagaaagt 
      gactccgag ttgagtgac gtggagctg aacgacgac 
      ggcaccgta acccccaag gccgagctc ctcggttgg 
      cactcctat ccttgggct tgctgcgac tgccagcac 
      accttcacc aactcggag gacctccgg gagcaccac 
      gccaccgtc cacgggcaa gcccccacg tatccttgc 
      gccgactgt ccgaaaatt tacacaaaa tacagcact 
      ttcctcacg cacgtgcgc gtccaccgc gtcaaactg 
      cgcttctgt tgcgatatt tgcgccaag tggttcccc 
      acagtcctg gcccagaag aagcacagg aacgagcac 
      ggcgatgag cgcccccat gcgtgcacc acgtgtggc 
      aaacgcttc cgcatgcaa tccgccctc atggtgcac 
      gcgaggtcg catctgccc gctgaatta aaaaacaag 
      caccaatgc gaccagtgc cccaagaaa ttcgggacg 
      aaacccaac ctcatggct cataagaga atccatgcg 
      ggggtgcgg gactacacg tgtgaccaa tgcgggaaa 
      agcttcgtc cagaaaggg aatctggac aaccacctt 
      ttaacacat acagccgcc aggccgttt gcctgttca 
      acctgtggt aaagccttc aagactttg gtccggctc 
      aggaagcac ggatcggtt cattcgggg ctgaagccc 
      caccagtgt gatgtttgc gggcggcag ttccgcgaa 
      aggggcact ctgaaggag caccaccgg attcacaca 
      ggggctatg cccttcacg tgcgagttc tgcggcaag 
      agttttcgg tttaagggg gttttgacc acgcatagg 
      agacagcat acgggggaa aggccctac tcatgccac 
      gaatgtcaa caccatttc accaattgg cccaattat 
      aacaaacat atgaagaga cggcatgga attaacact 
      agtgtgact gtgagaaaa ccgcagagt ataccaccg 
      acagggatg cctcatagg aatccccct gggactgtc 
      ctagcgccc ccacaaccc attacgata actgaaagt 
      tttgtcgaa acgccggcc tcgttttac cccgtttta 
      agtttgtat aatctccca gaagaattg ttacaacag 
      agagtgtga
    mRNA sequence
    • >TC014456 RA
      gtttgttta ttattgacc aaaaaagta atgtaaagt 
      ttgccggat tttttccga tttttcagc cggggggcc 
      gtttttgct cgatataat gtcatggag ggtcgctgc 
      cgtctgtgc gccgagttc ggcaccgat catctaaac 
      agtctcgat gatctcgat ctcatttta cgcattttt 
      caactcttt caagtccga atcacctcc gaggacaaa 
      ctcccaaca acaatctgc caattatgc gccgaaacg 
      gtgaaacaa acatgggag ttcaatcaa cacatccag 
      aaatcgcaa gagatcctc caggacatc gtcaacaca 
      gaactgcaa gcagcccac caggagagc aagctcgat 
      ttcacgctc acgctcgaa gaaagcaaa ctgaacgtg 
      gtcgaagtg accacatct tcagccgtt aaaacggaa 
      gatatcgta cttaagcct tcaccaaaa gtcaaaaaa 
      accggccgt aaaaaagaa agtgactcc gagttgagt 
      gacgtggag ctgaacgac gacggcacc gtaaccccc 
      aaggccgag ctcctcggt tggcactcc tatccttgg 
      gcttgctgc gactgccag cacaccttc accaactcg 
      gaggacctc cgggagcac cacgccacc gtccacggg 
      caagccccc acgtatcct tgcgccgac tgtccgaaa 
      atttacaca aaatacagc actttcctc acgcacgtg 
      cgcgtccac cgcgtcaaa ctgcgcttc tgttgcgat 
      atttgcgcc aagtggttc cccacagtc ctggcccag 
      aagaagcac aggaacgag cacggcgat gagcgcccc 
      catgcgtgc accacgtgt ggcaaacgc ttccgcatg 
      caatccgcc ctcatggtg cacgcgagg tcgcatctg 
      cccgctgaa ttaaaaaac aagcaccaa tgcgaccag 
      tgccccaag aaattcggg acgaaaccc aacctcatg 
      gctcataag agaatccat gcgggggtg cgggactac 
      acgtgtgac caatgcggg aaaagcttc gtccagaaa 
      gggaatctg gacaaccac cttttaaca catacagcc 
      gccaggccg tttgcctgt tcaacctgt ggtaaagcc 
      ttcaagact ttggtccgg ctcaggaag cacggatcg 
      gttcattcg gggctgaag ccccaccag tgtgatgtt 
      tgcgggcgg cagttccgc gaaaggggc actctgaag 
      gagcaccac cggattcac acaggggct atgcccttc 
      acgtgcgag ttctgcggc aagagtttt cggtttaag 
      ggggttttg accacgcat aggagacag catacgggg 
      gaaaggccc tactcatgc cacgaatgt caacaccat 
      ttcaccaat tggcccaat tataacaaa catatgaag 
      agacggcat ggaattaac actagtgtg actgtgaga 
      aaaccgcag agtatacca ccgacaggg atgcctcat 
      aggaatccc cctgggact gtcctagcg cccccacaa 
      cccattacg ataactgaa agttttgtc gaaacgccg 
      gcctcgttt taccccgtt ttaagtttg tataatctc 
      ccagaagaa ttgttacaa cagagagtg tgagagaaa 
      tttagagat aggcgtagc agtattatt tgattggta 
      ttaattaat agaaggctc caaaaaaca gtttatctt 
      caagcggtc gaaaattag aatgaaaaa agcgaaaaa 
      acgacacaa taaaattat tagccatta tttaaccgg 
      tgccactgt ggtgtaaca cattgtaca tcggtgaaa 
      attttcaaa atcaaaaat agaaatatg gccattaaa 
      cgtacaaaa gtaaacagt aaattttcg cttcaagtg 
      atccgcttg aagataaaa tagtaggta cgtatgtgt 
      cattaacag taggtgttt ttgggcttc gccccgttg 
      ccagggtcg gcctccagt ctttaggct tggcataat 
      aagaatcac ttctaccct taatgaaaa atctactat 
      ttttagata gaaaaacag tgatattta gcctctctc 
      tgtggacca gtgaagtcc aagtattga tctaataga 
      ttctgaatc gcgtaatca ttacagaaa ttatttgcg 
      tcgtacatt aattttaat tttacgttt tatttattt 
      ttacaatat ttctttaaa atacatgta caaacataa 
      taaaaaata ttacgtgtc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY