Status
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Gene information
General information
TC013381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10242388..10245155
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013381
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013381 RA
      MALRCPDLP FLPGYTFNP MLGKTKFNM DPKFDFIGD 
      GVPALVEKV RPNMFGPLS DKYPSLYPR GEVLELPSW 
      IAFDKQILC FDAFFKETL QEVRGAPFS LRRVKIYFF 
      LEDGTIQVI EPKVENSGI AQGTLICRH RIRFPAPMD 
      DNFYEVVDL NVGKEVEFY GRVFKITDC DKFTRNFLN 
      RCGIHVPDS LNIPGDPFL ELRNQENAR IKKRGKKKD 
      DSLGKFLEN DRKVLRFYG YWDDQETVF GYLHRLEIL 
      YYLADDTIE IREVSTEDC NKKTTFIFL HRDKIPKTY 
      KELPMPGAN CASTVLNVL GPGLQGGRF IKDPLSCGV 
      EVTEYYCEK DLFIGSVIN CYGRKLVLT DCDPYTREY 
      YRSMYGLNS LDAIAFPED KHAGVKVEQ KERELPPWN 
      GYGSYEDSA QNCITVEPK APHRDFKKF LNLDRIGID 
      SHILRFEAR MLSKIPENC TRHFVISYY LADDTIAVF 
      ERGIRNSGF DTKEFFARR SVALPNQGV YTSKPPITY 
      KSQDMYVGA TLIINSFKF VLIDADEYA LRYMEIHDH 
      QYPKANIKV IMGKIREKL RPTYKDFVA ENMPKESTT 
      IPYDTLKAK LLGILGDDF TEHEMITIA RAFPGDVDL 
      TEKYDREKI RAITLTELK RGLWDDLAR LREYFLMRD 
      PHKSGVLPR NECYIVLRA CRLPLEKEL VDKILQVIQ 
      KDNEGNLCY EDILNFVNR DICKLPDVE PISLKHELS 
      CIKVIEYEK YKLINWCEF NKFLDLEDT FKEQPVNNT 
      IERLQKNK
    CDS sequence
    • >TC013381 RA
      atggctttg cggtgcccc gatttgccc tttttgccg 
      ggctatacc ttcaaccca atgctggga aaaaccaaa 
      tttaatatg gaccccaaa ttcgatttt atcggtgat 
      ggggtccca gcattggtc gagaaagtc agaccgaat 
      atgttcggg ccactttcc gataaatac ccctctttg 
      taccccagg ggcgaagtt ctggaactc ccctcctgg 
      atcgcgttt gacaaacaa atcttgtgc ttcgacgcg 
      ttttttaag gagacgttg caggaggta cgtggtgcc 
      cccttttca ctgcgacgg gtcaaaatt tattttttc 
      ctcgaagat gggacgatt caggtgatt gagcccaaa 
      gttgaaaat agcgggatt gcgcaaggg actctaatt 
      tgtcggcac cgaatccgg tttccggcc cccatggat 
      gataatttt tacgaagtt gttgattta aatgttggc 
      aaagaggtc gaattctat ggccgggtt tttaaaatc 
      accgattgt gataaattc actcggaat tttcttaat 
      cggtgtggg attcacgtc ccggattca ttaaacatt 
      cccggggat cctttcttg gagttgcgt aatcaagaa 
      aatgccagg attaaaaaa cgagggaaa aagaaagac 
      gattccctt gggaagttt ctggaaaat gaccggaaa 
      gtgttacgt ttttatggc tattgggac gatcaggag 
      acagtcttt gggtatttg caccgtctg gaaatttta 
      tattaccta gcagacgat acgattgaa atccgggaa 
      gtttcgacc gaggattgt aacaagaaa accactttt 
      atttttttg catagagac aaaattcct aaaacttac 
      aaagaactg ccaatgccg ggcgccaac tgcgctagc 
      accgtttta aatgtttta gggccgggg ttgcaaggg 
      gggcgcttt atcaaagac cccttgtcg tgtggggtc 
      gaggtcacg gagtactac tgcgaaaag gatctgttt 
      attggaagt gtgattaat tgttacggg aggaagttg 
      gtactcact gactgtgac ccctatacc cgggagtac 
      tacagaagt atgtacggt ttgaacagt ttagacgcc 
      atagctttc cctgaggac aagcacgcg ggggttaaa 
      gtcgagcaa aaggagcgc gagttgccc ccttggaac 
      ggctacggt tcttatgag gactcggcc cagaattgt 
      atcacagtg gagcccaag gcgccccat cgagacttc 
      aaaaaattc ctcaacttg gatcgaatc gggatcgac 
      agtcacatt ttacgattc gaggcccga atgttgtcg 
      aaaataccc gagaactgt actcggcat tttgtcata 
      agttactac ttggctgac gacacgatt gctgtgttt 
      gagcgcggg attagaaat tcgggtttt gatacgaag 
      gagttcttc gcgcggagg tccgtcgct ctacccaat 
      caaggggtc tatacaagt aaaccgcca atcacgtac 
      aagtcccaa gacatgtat gtgggggct actttgatt 
      attaacagt tttaaattc gtactgata gacgcagac 
      gagtatgcg ctcagatat atggaaatt cacgatcat 
      cagtacccg aaagcgaac attaaagtt ataatgggg 
      aaaattcgc gaaaagctg agaccgact tacaaggat 
      tttgttgcg gaaaatatg ccgaaagag agtacaacc 
      attccttat gatactctc aaggcaaaa ttattgggg 
      attttgggc gacgatttc accgaacat gaaatgatc 
      acaatcgct cgggctttt cccggcgat gtcgactta 
      accgaaaag tatgatcgt gaaaaaata agggcaatc 
      actctgacg gaattaaag cgggggctt tgggacgat 
      ttggcccga ttgagggaa tatttcttg atgagagac 
      ccccacaaa tccggggtt ttacccaga aatgagtgc 
      tacattgta ttacgggcc tgcagactg cctttggag 
      aaagaactc gtcgacaaa attttgcaa gtaatacag 
      aaagataac gaaggtaat ctctgctac gaggatatt 
      ttgaatttc gtcaatcgg gatatttgt aaattacca 
      gacgttgaa cccattagt ttgaaacat gagttgagt 
      tgcatcaaa gtgatcgag tacgaaaag tataagttg 
      atcaactgg tgcgaattc aacaaattt ttggacttg 
      gaagacact ttcaaggag caaccagtg aataacacg 
      attgagcga ctccaaaaa aataaataa
    mRNA sequence
    • >TC013381 RA
      caaccagtt tccgtcaaa ttcacgaca cgatggctt 
      tgcggtgcc ccgatttgc cctttttgc cgggctata 
      ccttcaacc caatgctgg gaaaaacca aatttaata 
      tggacccca aattcgatt ttatcggtg atggggtcc 
      cagcattgg tcgagaaag tcagaccga atatgttcg 
      ggccacttt ccgataaat acccctctt tgtacccca 
      ggggcgaag ttctggaac tcccctcct ggatcgcgt 
      ttgacaaac aaatcttgt gcttcgacg cgtttttta 
      aggagacgt tgcaggagg tacgtggtg ccccctttt 
      cactgcgac gggtcaaaa tttattttt tcctcgaag 
      atgggacga ttcaggtga ttgagccca aagttgaaa 
      atagcggga ttgcgcaag ggactctaa tttgtcggc 
      accgaatcc ggtttccgg cccccatgg atgataatt 
      tttacgaag ttgttgatt taaatgttg gcaaagagg 
      tcgaattct atggccggg tttttaaaa tcaccgatt 
      gtgataaat tcactcgga attttctta atcggtgtg 
      ggattcacg tcccggatt cattaaaca ttcccgggg 
      atcctttct tggagttgc gtaatcaag aaaatgcca 
      ggattaaaa aacgaggga aaaagaaag acgattccc 
      ttgggaagt ttctggaaa atgaccgga aagtgttac 
      gtttttatg gctattggg acgatcagg agacagtct 
      ttgggtatt tgcaccgtc tggaaattt tatattacc 
      tagcagacg atacgattg aaatccggg aagtttcga 
      ccgaggatt gtaacaaga aaaccactt ttatttttt 
      tgcatagag acaaaattc ctaaaactt acaaagaac 
      tgccaatgc cgggcgcca actgcgcta gcaccgttt 
      taaatgttt tagggccgg ggttgcaag gggggcgct 
      ttatcaaag accccttgt cgtgtgggg tcgaggtca 
      cggagtact actgcgaaa aggatctgt ttattggaa 
      gtgtgatta attgttacg ggaggaagt tggtactca 
      ctgactgtg acccctata cccgggagt actacagaa 
      gtatgtacg gtttgaaca gtttagacg ccatagctt 
      tccctgagg acaagcacg cgggggtta aagtcgagc 
      aaaaggagc gcgagttgc ccccttgga acggctacg 
      gttcttatg aggactcgg cccagaatt gtatcacag 
      tggagccca aggcgcccc atcgagact tcaaaaaat 
      tcctcaact tggatcgaa tcgggatcg acagtcaca 
      ttttacgat tcgaggccc gaatgttgt cgaaaatac 
      ccgagaact gtactcggc attttgtca taagttact 
      acttggctg acgacacga ttgctgtgt ttgagcgcg 
      ggattagaa attcgggtt ttgatacga aggagttct 
      tcgcgcgga ggtccgtcg ctctaccca atcaagggg 
      tctatacaa gtaaaccgc caatcacgt acaagtccc 
      aagacatgt atgtggggg ctactttga ttattaaca 
      gttttaaat tcgtactga tagacgcag acgagtatg 
      cgctcagat atatggaaa ttcacgatc atcagtacc 
      cgaaagcga acattaaag ttataatgg ggaaaattc 
      gcgaaaagc tgagaccga cttacaagg attttgttg 
      cggaaaata tgccgaaag agagtacaa ccattcctt 
      atgatactc tcaaggcaa aattattgg ggattttgg 
      gcgacgatt tcaccgaac atgaaatga tcacaatcg 
      ctcgggctt ttcccggcg atgtcgact taaccgaaa 
      agtatgatc gtgaaaaaa taagggcaa tcactctga 
      cggaattaa agcgggggc tttgggacg atttggccc 
      gattgaggg aatatttct tgatgagag acccccaca 
      aatccgggg ttttaccca gaaatgagt gctacattg 
      tattacggg cctgcagac tgcctttgg agaaagaac 
      tcgtcgaca aaattttgc aagtaatac agaaagata 
      acgaaggta atctctgct acgaggata ttttgaatt 
      tcgtcaatc gggatattt gtaaattac cagacgttg 
      aacccatta gtttgaaac atgagttga gttgcatca 
      aagtgatcg agtacgaaa agtataagt tgatcaact 
      ggtgcgaat tcaacaaat ttttggact tggaagaca 
      ctttcaagg agcaaccag tgaataaca cgattgagc 
      gactccaaa aaaataaat aaataaaac actgccctt 
      gtgaaactt ttgtcgtgt gtttttgtt gttgtgtaa 
      aagtttaca ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY