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Gene information
General information
TC014410 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10199682..10201308
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014410
    iBeetle screen iB_09099

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014410 RA
      MAPKNDPEL IALRKELDE LYNTLKDEQ KKQADTTLE 
      SACSGVADA PRVKLITRK LMKGHLNKV NSVHYSGDN 
      RHCVTGSLD GKLIIWDTY TGNKMQIIP LRSAWVMSV 
      AYAPSGNFV ACGGMDNMC TVYDLNNRD SSGVAKMVR 
      ELAGYDGFL SSCRFLGDR NIITGSGDM KICKWDLET 
      GRKTSDFVA HNGDVVSIS LSPDGNSFV TGSVDKTCR 
      LWDMREEKP RQTFFGHES DVNSVCFHP SGYAFATGS 
      EDKSARMFD IRSDQQLAH YKPPTANSG FTSCGLSVS 
      GRILFCGSD DNNVHMWDT LKNQHNGTL SGHENRVTS 
      LSVAPNGIA VVTSSWDQH VRVWG
    CDS sequence
    • >TC014410 RA
      atggcgcca aaaaacgac ccggaactc atcgcctta 
      cgcaaagag ctcgacgag ctttacaat actctgaag 
      gacgagcag aagaagcaa gctgacacc accctcgaa 
      agcgcttgc agcggcgtc gccgacgct ccgagagtc 
      aaactcatc accaggaag ctgatgaag ggccatttg 
      aacaaagtc aactcggtg cattacagc ggagataac 
      aggcactgc gttaccggc tcgcttgac gggaaattg 
      atcatttgg gacacgtac acggggaac aaaatgcag 
      attataccc ctgaggtcg gcctgggtg atgagcgtg 
      gcgtacgca ccgtcgggg aattttgtc gcttgtggt 
      ggcatggac aacatgtgc accgtgtac gacctgaac 
      aaccgcgac tcctccggc gtggccaag atggtgcgc 
      gagctggcg ggttacgac ggcttcctc agctcgtgc 
      cgcttcctg ggtgaccgc aacatcatc acaggctcc 
      ggcgacatg aaaatttgc aagtgggac ttggagacc 
      ggtcgcaaa acctccgac ttcgtcgcc cacaacggc 
      gacgtcgtc tccatcagt ctctccccg gatggcaac 
      agtttcgtg acgggaagt gtcgacaag acttgccgg 
      ttgtgggac atgagagag gagaaaccg agacaaacc 
      ttcttcgga cacgaatcc gacgttaat tcagtttgc 
      tttcatcca agcggttac gctttcgcc accggatct 
      gaggacaag tccgcgaga atgtttgat ataaggagc 
      gaccagcag ttggcgcat tataagccg ccgacggcc 
      aacagcggg ttcacttcg tgcgggttg tcggtcagc 
      ggaaggatt ttgttctgt gggtcggat gataacaac 
      gtccacatg tgggacacc ctcaagaac caacataat 
      gggactttg tcgggacac gaaaatagg gtcacgtcg 
      ctgtcggtt gccccaaat ggcattgcc gtggtcacc 
      tccagttgg gaccagcat gtgagggtt tggggttga
    mRNA sequence
    • >TC014410 RA
      actaattag attacatgc ttggcttcg ggccacttg 
      tcagttgcg aagatacac tcaggtgca aagggtgag 
      agtgtgcga aatggcgcc aaaaaacga cccggaact 
      catcgcctt acgcaaaga gctcgacga gctttacaa 
      tactctgaa ggacgagca gaagaagca agctgacac 
      caccctcga aagcgcttg cagcggcgt cgccgacgc 
      tccgagagt caaactcat caccaggaa gctgatgaa 
      gggccattt gaacaaagt caactcggt gcattacag 
      cggagataa caggcactg cgttaccgg ctcgcttga 
      cgggaaatt gatcatttg ggacacgta cacggggaa 
      caaaatgca gattatacc cctgaggtc ggcctgggt 
      gatgagcgt ggcgtacgc accgtcggg gaattttgt 
      cgcttgtgg tggcatgga caacatgtg caccgtgta 
      cgacctgaa caaccgcga ctcctccgg cgtggccaa 
      gatggtgcg cgagctggc gggttacga cggcttcct 
      cagctcgtg ccgcttcct gggtgaccg caacatcat 
      cacaggctc cggcgacat gaaaatttg caagtggga 
      cttggagac cggtcgcaa aacctccga cttcgtcgc 
      ccacaacgg cgacgtcgt ctccatcag tctctcccc 
      ggatggcaa cagtttcgt gacgggaag tgtcgacaa 
      gacttgccg gttgtggga catgagaga ggagaaacc 
      gagacaaac cttcttcgg acacgaatc cgacgttaa 
      ttcagtttg ctttcatcc aagcggtta cgctttcgc 
      caccggatc tgaggacaa gtccgcgag aatgtttga 
      tataaggag cgaccagca gttggcgca ttataagcc 
      gccgacggc caacagcgg gttcacttc gtgcgggtt 
      gtcggtcag cggaaggat tttgttctg tgggtcgga 
      tgataacaa cgtccacat gtgggacac cctcaagaa 
      ccaacataa tgggacttt gtcgggaca cgaaaatag 
      ggtcacgtc gctgtcggt tgccccaaa tggcattgc 
      cgtggtcac ctccagttg ggaccagca tgtgagggt 
      ttggggttg aaacgaaat gccatcaat gtgattcaa 
      taaataaac ttttcttcc aaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY