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Gene information
General information
TC014370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9859082..9876472
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014370
    iBeetle screen iB_09096

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014370 RA
      MASVGFRWL DILEKEFDK AFVDLDLAI GELDSEEPD 
      VVFNVRQKL CSLSSCFAQ LTHKAQTIF QNSAKIEAE 
      LIHMRAELV ESKAKREVL EQELHNLLL QLHSSQLSQ 
      LPDSLGNRK QDPNFKPDV NNIKKKLEA ELRQSPIRN 
      IRLNKESDE LKFSPSLME TAQCRAENE QLQVENAAL 
      RKSILALNS EVYGAKLAA KYLDKELAG RIQQLQLLG 
      REMRGDVRD KLWRQLESE ILLQRHKTV VRACRRNSI 
      LNNSEKTCP KPDIDPINE RTEHFGDIR TVVVKRSPS 
      QGLGISITG GREHGVPIL ISELEPNGP AALSEQLYI 
      GDAILSVND RDLRQACHR EAVDILQQQ SGDCTLQVQ 
      YIAADDSDT SLEEDGYNF RFFDGEVCD PNNHSSLNP 
      SNSLDMLNT PTAPRTPES SSAASRIVS RDSIPNSPT 
      IPKVQKLIV LEETEDFDA NIFNYNLNT NGNLETYKP 
      TTTKDIKIA DDLNCNTTP STDTVETTD LVLIDNATN 
      NVIKLEDFK KVDIIDDEN FNCSTNSLN SENFSSPMQ 
      TSADVFLDS ELLSTDVAS NDSQSPKNI QAELSATKP 
      TFTYQEPQF CSKPQIYTS NESLSNPKS DQSLLNSAE 
      SCGSSPVHK SSNRDYKKV QRKHKKKYF GVKTLQSIF 
      NKHDGYSDI SSTASELGD EIIEPKDNK TEMKEKEPS 
      YKCGKVYTE TSPQFEENI HNILTDIFV KNFSTESNV 
      ASISLSKPI GTKKYFSLH GQNLRVGKA LRLCPNEDY 
      SEQVENEQR KEKLLARYV YNANSINAD GVGDPDFGT 
      PV
    CDS sequence
    • >TC014370 RA
      atggctagt gtcgggttt aggtggttg gatatctta 
      gaaaaagaa ttcgataag gctttcgta gacttagac 
      ttggcaata ggtgaactc gattcagaa gagcctgat 
      gttgtcttc aacgtccgc caaaaactg tgcagcctt 
      agctcatgt tttgcacaa ctgacacac aaggcacag 
      accattttt caaaacagc gccaagata gaggccgaa 
      ctaatccac atgagggcc gaattggtg gaatcgaaa 
      gctaagagg gaagtttta gagcaagaa ctgcacaat 
      ttgttgctc cagttgcac tcgtcgcag ctttcgcaa 
      ctgcccgac agtctcggt aatcgcaaa caagaccca 
      aacttcaaa cccgacgta aataacatc aagaaaaaa 
      ctagaagcc gagctccga cagagtccc atacgtaac 
      attaggcta aacaaagaa tcggacgaa ctcaaattc 
      agcccttcg ctgatggaa acggcacaa tgtcgggca 
      gagaacgaa caactccag gtggaaaac gcggcgtta 
      agaaaatcc atcctggca ttgaacagc gaggtttac 
      ggcgcaaag ctcgccgcg aaatattta gacaaggaa 
      ttagcgggg agaatacag caattacaa ctcttaggg 
      agagaaatg cgaggagat gttcgtgat aaattgtgg 
      cgtcaactg gaatctgaa attctactt cagagacac 
      aaaacggta gtgcgagcg tgtaggaga aatagcatt 
      ttgaataat tccgaaaag acctgcccc aagcccgac 
      atcgatccg atcaacgaa cgcacggag catttcggg 
      gatataagg accgtagtg gtgaagcgg agccccagc 
      cagggactg ggaattagt ataacgggc gggagagag 
      cacggggtg ccgattttg atctcggag ctggagccc 
      aacgggccc gcggcctta tcagagcag ctctacata 
      ggagacgcg atcttatca gtgaacgac cgcgacttg 
      cgacaagcc tgccaccga gaggctgta gatatcctc 
      cagcaacaa tcgggcgac tgcacgtta caagtgcaa 
      tatatcgcc gcggatgac agcgacact tcgctcgaa 
      gaggacggt tacaatttc cggtttttc gacggagaa 
      gtgtgcgac ccgaacaat cactctagt ttaaaccct 
      tcaaactct ctagacatg ttaaatacc cccacagca 
      cctagaact cccgaatca agttcggcg gcaagccgg 
      atagtttca agagatagc atccccaac agccccaca 
      atccccaaa gtgcaaaaa ctgatagtt ttggaagaa 
      accgaagac ttcgacgcc aacatcttc aactacaac 
      ctcaacacc aacggcaac ctggagacc tacaaaccc 
      accacaacc aaagacatc aaaatcgcc gacgacctg 
      aactgcaac accactcca agcaccgac acggtcgaa 
      accacggac ttggtcctc atcgacaac gccacgaac 
      aacgtcatc aagctcgaa gacttcaaa aaagtcgac 
      ataatcgac gacgagaac tttaactgt tcaacgaac 
      tccctcaac tcggagaac ttctcctcg ccgatgcag 
      acaagcgcg gatgttttt ctcgattcg gagttgtta 
      tcgactgac gtagcttca aacgactcg caatcgccc 
      aaaaacatc caagcggag ctgagtgcc acaaagccc 
      actttcacg taccaggaa ccgcagttc tgttccaaa 
      ccccagatt tacacctcg aacgagtcg ctgtcgaac 
      cccaagagc gaccaaagc ctcctcaac tcggctgag 
      agctgcggc agctcgccg gtgcacaaa agcagcaat 
      cgggactac aagaaggtg cagaggaag cacaagaag 
      aagtacttt ggggtgaag acgctacag agcattttt 
      aataaacac gacggctat tcggacatt tcgagcacg 
      gcgtcggag ctcggggac gaaataatt gaaccaaaa 
      gacaataaa accgaaatg aaggagaag gaaccgagc 
      tacaagtgt ggcaaggtt tacactgag actagcccg 
      cagttcgag gagaacatt cacaacatt ttgacggac 
      attttcgtg aaaaacttt agtaccgaa agcaacgtc 
      gcgagcatt tctctgagt aaaccgatc gggacgaag 
      aaatatttc agcttgcac ggccagaat ttgcgcgtg 
      ggcaaagcg ctgaggctt tgccccaac gaggattat 
      tccgaacaa gtggagaac gagcagagg aaggagaaa 
      cttttagcg agatatgtc tacaatgcg aattcaata 
      aacgcggac ggtgtaggg gaccctgac tttggaacg 
      cctgtttag
    mRNA sequence
    • >TC014370 RA
      atggctagt gtcgggttt aggtggttg gatatctta 
      gaaaaagaa ttcgataag gctttcgta gacttagac 
      ttggcaata ggtgaactc gattcagaa gagcctgat 
      gttgtcttc aacgtccgc caaaaactg tgcagcctt 
      agctcatgt tttgcacaa ctgacacac aaggcacag 
      accattttt caaaacagc gccaagata gaggccgaa 
      ctaatccac atgagggcc gaattggtg gaatcgaaa 
      gctaagagg gaagtttta gagcaagaa ctgcacaat 
      ttgttgctc cagttgcac tcgtcgcag ctttcgcaa 
      ctgcccgac agtctcggt aatcgcaaa caagaccca 
      aacttcaaa cccgacgta aataacatc aagaaaaaa 
      ctagaagcc gagctccga cagagtccc atacgtaac 
      attaggcta aacaaagaa tcggacgaa ctcaaattc 
      agcccttcg ctgatggaa acggcacaa tgtcgggca 
      gagaacgaa caactccag gtggaaaac gcggcgtta 
      agaaaatcc atcctggca ttgaacagc gaggtttac 
      ggcgcaaag ctcgccgcg aaatattta gacaaggaa 
      ttagcgggg agaatacag caattacaa ctcttaggg 
      agagaaatg cgaggagat gttcgtgat aaattgtgg 
      cgtcaactg gaatctgaa attctactt cagagacac 
      aaaacggta gtgcgagcg tgtaggaga aatagcatt 
      ttgaataat tccgaaaag acctgcccc aagcccgac 
      atcgatccg atcaacgaa cgcacggag catttcggg 
      gatataagg accgtagtg gtgaagcgg agccccagc 
      cagggactg ggaattagt ataacgggc gggagagag 
      cacggggtg ccgattttg atctcggag ctggagccc 
      aacgggccc gcggcctta tcagagcag ctctacata 
      ggagacgcg atcttatca gtgaacgac cgcgacttg 
      cgacaagcc tgccaccga gaggctgta gatatcctc 
      cagcaacaa tcgggcgac tgcacgtta caagtgcaa 
      tatatcgcc gcggatgac agcgacact tcgctcgaa 
      gaggacggt tacaatttc cggtttttc gacggagaa 
      gtgtgcgac ccgaacaat cactctagt ttaaaccct 
      tcaaactct ctagacatg ttaaatacc cccacagca 
      cctagaact cccgaatca agttcggcg gcaagccgg 
      atagtttca agagatagc atccccaac agccccaca 
      atccccaaa gtgcaaaaa ctgatagtt ttggaagaa 
      accgaagac ttcgacgcc aacatcttc aactacaac 
      ctcaacacc aacggcaac ctggagacc tacaaaccc 
      accacaacc aaagacatc aaaatcgcc gacgacctg 
      aactgcaac accactcca agcaccgac acggtcgaa 
      accacggac ttggtcctc atcgacaac gccacgaac 
      aacgtcatc aagctcgaa gacttcaaa aaagtcgac 
      ataatcgac gacgagaac tttaactgt tcaacgaac 
      tccctcaac tcggagaac ttctcctcg ccgatgcag 
      acaagcgcg gatgttttt ctcgattcg gagttgtta 
      tcgactgac gtagcttca aacgactcg caatcgccc 
      aaaaacatc caagcggag ctgagtgcc acaaagccc 
      actttcacg taccaggaa ccgcagttc tgttccaaa 
      ccccagatt tacacctcg aacgagtcg ctgtcgaac 
      cccaagagc gaccaaagc ctcctcaac tcggctgag 
      agctgcggc agctcgccg gtgcacaaa agcagcaat 
      cgggactac aagaaggtg cagaggaag cacaagaag 
      aagtacttt ggggtgaag acgctacag agcattttt 
      aataaacac gacggctat tcggacatt tcgagcacg 
      gcgtcggag ctcggggac gaaataatt gaaccaaaa 
      gacaataaa accgaaatg aaggagaag gaaccgagc 
      tacaagtgt ggcaaggtt tacactgag actagcccg 
      cagttcgag gagaacatt cacaacatt ttgacggac 
      attttcgtg aaaaacttt agtaccgaa agcaacgtc 
      gcgagcatt tctctgagt aaaccgatc gggacgaag 
      aaatatttc agcttgcac ggccagaat ttgcgcgtg 
      ggcaaagcg ctgaggctt tgccccaac gaggattat 
      tccgaacaa gtggagaac gagcagagg aaggagaaa 
      cttttagcg agatatgtc tacaatgcg aattcaata 
      aacgcggac ggtgtaggg gaccctgac tttggaacg 
      cctgtttag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY