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Gene information
General information
TC033137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9476532..9478659
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033137
    iBeetle screen iB_09094

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033137 RA
      MLTRIPKVS LLLILLLVS TRAQNLVHR HLPSIAVVE 
      YEQITQPSF TTRDAQIKN AEKYIEIID ELSRKYHLI 
      LVIFPEATL DYFKSKQDL IDNGAEFKQ HIVPCNQTN 
      IPVFLKMLS CAAQKYHVT IVFNLIEKE AQRVYTSDA 
      LLNIDGTLE FSLKRENRI GTGALHNKP VAFAIPVRF 
      IFSIVSGAF LRQPNFDFT PTSFVTGFS FSYEKIVNR 
      NSKRNYVVC SNWISQLPF LTSLQVVQM WAQEFNITV 
      FMSGANNPA KGQGGTAIY HREDGPILM EIGAKRGTK 
      AYVYHFINN TSGALAESV EEKSVDELA KEMDNFYLE 
      QDPDLDRYK SLVLRKKES FVYLCNDNV KDNYFCCSF 
      DIKLSINTS VTNKKRYTY HLVAYKGYK TYHNIFRPG 
      GIEVCAVIA CLNSKLKSC GQRFKNYDE IGWPITFEK 
      LVIKATFGM PRKEKIYYS SQFPNSLLS SIRPIHPDF 
      TSWSSQIIF DAQNKSYLE RTFALLEPQ SKILTFGIF 
      GRNFTLHDQ RSKGQKLSF HPLGIVLSL IIINY
    CDS sequence
    • >TC033137 RA
      atgttaaca cgcatcccg aaagtttcc cttttgtta 
      attttacta ttggtgagc acaagagct caaaatttg 
      gtgcacaga catctccca agcatcgca gttgtggaa 
      tacgaacaa ataacacaa ccatctttc acgacacga 
      gatgcacag ataaaaaac gccgaaaaa tacattgag 
      ataatagat gaactgtcc aggaaatac catcttata 
      ttggtgatt ttccccgag gctacttta gactacttc 
      aaaagcaag caagacctc attgacaat ggtgccgaa 
      ttcaaacaa catattgta ccttgtaat caaaccaac 
      atcccagta ttcctcaaa atgttgtca tgtgctgcc 
      caaaaatat catgtgacg attgtcttt aatttaatc 
      gaaaaggaa gcccagaga gtatatact tctgatgca 
      ttgcttaac attgatgga acccttgaa tttagcctt 
      aaacgagaa aatcggatc ggaaccggc gctttacac 
      aacaaacca gtagcattt gcaatccct gtccgtttc 
      atttttagc atcgtttct ggagcattt ctgagacaa 
      cctaatttc gacttcaca cccacatct ttcgtcaca 
      ggatttagc ttttcatac gaaaaaatt gtaaatcgg 
      aacagcaaa agaaattat gttgtttgt agcaactgg 
      atatcgcag ttaccgttt ttgacatca ctgcaagtt 
      gtgcaaatg tgggcacaa gaatttaat atcactgtg 
      tttatgtcg ggagctaat aatcctgca aaaggacaa 
      ggaggaact gctatttac catcgggaa gatgggcct 
      attttgatg gaaattggt gcaaaaaga gggactaaa 
      gcgtatgtt taccatttt ataaataac acgtcggga 
      gcgctggca gagagtgtc gaggaaaaa agcgttgac 
      gagttggca aaagagatg gataatttt tacttagaa 
      caggaccct gatttggat agatacaaa tcgctagtt 
      ttaagaaaa aaagaatct tttgtgtat ctttgcaat 
      gacaatgtt aaagacaat tatttttgc tgctcgttc 
      gacattaaa ctatccata aacacctca gttacaaac 
      aagaaacga tacacctac cacttggtg gcctataaa 
      ggttacaaa acctaccac aatattttt cgacctgga 
      ggcattgaa gtttgtgcc gtgatcgcc tgcttaaat 
      tccaaactg aaatcttgt ggtcaacga tttaaaaac 
      tacgatgaa attggatgg cccataacc tttgaaaaa 
      ctcgttatt aaggcaact tttggtatg cccaggaaa 
      gaaaaaatt tactacagt tcacagttt ccgaattct 
      ttgctcagt tctattcga ccaattcat ccagacttt 
      acgtcctgg agctcacag attatcttc gacgctcaa 
      aacaaatcg tatctagaa cgaacattc gctcttctc 
      gagccccaa agcaaaata ctgactttt ggaattttt 
      ggaagaaac tttacgttg cacgatcag aggtcaaag 
      ggtcaaaaa ttgtccttt cacccgctt ggcatagta 
      ttgagctta attattatt aattattaa
    mRNA sequence
    • >TC033137 RA
      gacaatagt tctttaaac gagctcgct tccggtgga 
      tggggatgt ttgtactta ccaaactca tcttcctgt 
      cttccactc gctgactct ccattgtgc aaagctaaa 
      aaattgaca atgatgaat caataagtc ggcgaacgg 
      agaggaaaa tattactga caggatatg agggtcatt 
      tagggaagc agggccggg ttgggtttt tttaaaaat 
      atgtttata agttgacgc acaacaatg acactttta 
      atatgtcaa aaaatgtta acacgcatc ccgaaagtt 
      tcccttttg ttaatttta ctattggtg agcacaaga 
      gctcaaaat ttggtgcac agacatctc ccaagcatc 
      gcagttgtg gaatacgaa caaataaca caaccatct 
      ttcacgaca cgagatgca cagataaaa aacgccgaa 
      aaatacatt gagataata gatgaactg tccaggaaa 
      taccatctt atattggtg attttcccc gaggctact 
      ttagactac ttcaaaagc aagcaagac ctcattgac 
      aatggtgcc gaattcaaa caacatatt gtaccttgt 
      aatcaaacc aacatccca gtattcctc aaaatgttg 
      tcatgtgct gcccaaaaa tatcatgtg acgattgtc 
      tttaattta atcgaaaag gaagcccag agagtatat 
      acttctgat gcattgctt aacattgat ggaaccctt 
      gaatttagc cttaaacga gaaaatcgg atcggaacc 
      ggcgcttta cacaacaaa ccagtagca tttgcaatc 
      cctgtccgt ttcattttt agcatcgtt tctggagca 
      tttctgaga caacctaat ttcgacttc acacccaca 
      tctttcgtc acaggattt agcttttca tacgaaaaa 
      attgtaaat cggaacagc aaaagaaat tatgttgtt 
      tgtagcaac tggatatcg cagttaccg tttttgaca 
      tcactgcaa gttgtgcaa atgtgggca caagaattt 
      aatatcact gtgtttatg tcgggagct aataatcct 
      gcaaaagga caaggagga actgctatt taccatcgg 
      gaagatggg cctattttg atggaaatt ggtgcaaaa 
      agagggact aaagcgtat gtttaccat tttataaat 
      aacacgtcg ggagcgctg gcagagagt gtcgaggaa 
      aaaagcgtt gacgagttg gcaaaagag atggataat 
      ttttactta gaacaggac cctgatttg gatagatac 
      aaatcgcta gttttaaga aaaaaagaa tcttttgtg 
      tatctttgc aatgacaat gttaaagac aattatttt 
      tgctgctcg ttcgacatt aaactatcc ataaacacc 
      tcagttaca aacaagaaa cgatacacc taccacttg 
      gtggcctat aaaggttac aaaacctac cacaatatt 
      tttcgacct ggaggcatt gaagtttgt gccgtgatc 
      gcctgctta aattccaaa ctgaaatct tgtggtcaa 
      cgatttaaa aactacgat gaaattgga tggcccata 
      acctttgaa aaactcgtt attaaggca acttttggt 
      atgcccagg aaagaaaaa atttactac agttcacag 
      tttccgaat tctttgctc agttctatt cgaccaatt 
      catccagac tttacgtcc tggagctca cagattatc 
      ttcgacgct caaaacaaa tcgtatcta gaacgaaca 
      ttcgctctt ctcgagccc caaagcaaa atactgact 
      tttggaatt tttggaaga aactttacg ttgcacgat 
      cagaggtca aagggtcaa aaattgtcc tttcacccg 
      cttggcata gtattgagc ttaattatt attaattat 
      taataataa aataacgtt ctgtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY