Status
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Gene information
General information
TC014345 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9447471..9455201
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014345
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014345 RA
      MAQGTLLIL VCVTTVATF AFPHPGEDV ITQPKPGEE 
      YVFVQTTGS GARPIARKD KLTSEERPF ETAHKLPPS 
      LTYILNEND DDVKTKTKR AANPAEAPA PAPAGAIKL 
      DVQKLLEKY KTELKTSTT EAPKTTART TRRRGNKRT 
      KKEIKEVEV TSPSPVLTT GASAPKKVD DDIPLVASG 
      SEKQRSRIQ IKKAPNGQE YEYEYVYYY YDEDDDAKV 
      TNAHDGPAK AANKSRGAV ERTSAAPEA NEVVPSSRG 
      KTRGRQLGE EEVSEERLP ANTRFPPRS RNLNTTPLP 
      EEETKATRG RGRGRPTTE AAPSTDSVS DETQGSRGR 
      TRANVRRPS LELVDNASF NTHPGSEPS GSSPSFPTD 
      LPAGPVRFL GATPNESVD PPEPIATSD NPPEPKKEE 
      TSTETAEET GTTAMSAMD KVALDLYAI LQGTQKLSG 
      EEGDFSSEE NATDTEVTT LEATTEQII ETTTPTTTT 
      TTTTTTTPA PTTTTTTEA PIGRGRFKG RGRKTTHSA 
      STTEAAHAE TKPKSKFSR PSFGGRHRA GARSTTEAA 
      VEHDASKEE SKPVSHSRP TLSRNRFGA TRSRTRTTA 
      APSDSNDDS PSSTSAPVS RPSLTRPRS SFNLRTRGR 
      STTTQSPEN ESHDEETAS SSEPASTTA RSRLVQGSG 
      VRPLRPGPR INLARSRPG QSTTTTEAP AEDHATGDA 
      EQPTNTNSE ASEEKDKET SAPVDNSPL GRLKNKHRV 
      NVQARPKAA ASTPVQVRR VNPLLARRR PGQTTEAAS 
      SEAPQEVST EAAEAEEHE ETEAAVEAS STTTEEPRG 
      LNKLLAGRR RLATRPAAH H
    CDS sequence
    • >TC014345 RA
      atggcacaa ggcactttg ctcatactt gtatgcgta 
      acgaccgta gcaactttc gctttcccc catcctgga 
      gaggacgta atcacacaa cctaaacct ggggaagag 
      tacgttttc gtgcaaacc acgggttca ggcgcccgt 
      cccatagca cgaaaagac aagctaaca agtgaggag 
      cgtcctttc gaaaccgcc cacaaactt ccaccatct 
      ctcacatac atcctgaac gaaaacgac gacgatgtt 
      aagactaag accaaacga gcggccaat ccggccgaa 
      gccccggct cccgccccc gctggtgcg atcaaacta 
      gacgtgcaa aaactttta gaaaaatac aagaccgaa 
      ctcaagact agcacgacc gaagctccg aaaaccaca 
      gcgagaacg acgcggagg agaggcaac aagaggacg 
      aaaaaggag attaaggaa gttgaagtc actagtcct 
      agccccgtt ttgacgact ggagccagc gcccccaag 
      aaagtcgac gatgatatt cctctagtg gctagtggg 
      agcgagaaa cagcgatcg cggattcag atcaaaaag 
      gcacccaat gggcaggag tacgaatac gagtacgtt 
      tattattac tacgacgaa gatgatgat gcgaaagtg 
      accaatgcc catgatggg cctgctaag gctgcgaac 
      aagagtcga ggggctgtg gagcgaacc agtgcagct 
      cctgaagcc aacgaagtt gtgccctcg tcccgcggc 
      aagactaga ggccgccaa ctgggcgag gaagaagtc 
      agcgaggaa aggcttcct gctaatacg aggtttcct 
      ccaagaagt cgcaatctg aacactacg cctttaccg 
      gaagaggaa actaaagcg acaagaggt cggggaaga 
      ggaaggcca actactgag gctgcacct agcactgat 
      agtgtttcg gacgaaact caggggtct cgggggcgc 
      acgcgtgcc aacgtgaga aggccttcc ttggaattg 
      gttgacaat gcctccttt aacacccat ccaggatcc 
      gaaccttcc ggaagcagc ccgtccttc ccgacagac 
      cttccagct ggaccggta cgtttcttg ggagcaacg 
      cctaatgaa agcgtggat ccacctgaa cccatcgcc 
      acgtccgac aatccccca gaaccaaag aaagaagaa 
      acctcgact gaaaccgcc gaagaaacc gggacaaca 
      gccatgtca gcgatggac aaagtggcc ctcgacctc 
      tatgccatc ctccaaggg actcagaaa ctctccgga 
      gaagagggc gatttcagt agcgaggaa aatgcaact 
      gataccgag gtcacaact ttggaagcc acgaccgaa 
      caaattatt gagactaca actcccaca actacgaca 
      acaaccacc acaaccaca acaccagct cctacgacc 
      acaaccacg acggaagca ccaatcgga cgaggaaga 
      tttaaggga cgtgggagg aaaactacg cattctgcg 
      tcgacgacc gaagctgca catgcagaa acaaaacca 
      aaaagtaaa ttcagtaga cccagcttt ggagggaga 
      cacagagct ggggctagg tcgaccacg gaggcggca 
      gttgaacat gacgcttcc aaggaggaa tccaagcca 
      gtgagccac tctcggccc actttaagt cgaaatcgc 
      ttcggcgcg acgcgctcc aggacccga acaactgct 
      gcaccttct gactccaac gacgactct cctagcagc 
      acctcagcc ccagtatca agaccatct ctcacacgc 
      cctcgctca tccttcaat cttcgcact agaggacgt 
      tccaccacc acacaatca cccgaaaat gaatctcac 
      gacgaggaa actgcttcc agctccgaa cctgcgagc 
      accaccgcc agaagtaga ctcgttcaa ggcagtggc 
      gtaagaccc ctaagacct ggacctcga ataaatcta 
      gccagaagc cgccctgga caaagtaca accaccacg 
      gaagctccc gccgaagac catgcaacg ggagatgcg 
      gaacaacca actaacacg aactctgaa gcttcggaa 
      gaaaaagat aaagaaact tcggctcca gtagataat 
      agtccttta ggtagattg aaaaacaag caccgggtg 
      aacgtgcaa gcccggccc aaagctgca gcgagcact 
      ccggtgcaa gtccgtcgt gtgaatcca ctgcttgca 
      aggaggaga cctgggcaa actacggaa gctgcgtct 
      agtgaagca ccacaagaa gtgagcacc gaagcagct 
      gaagctgaa gaacacgaa gaaaccgaa gctgcagtt 
      gaagcgtcc agtactacc accgaggaa cctagagga 
      ttaaacaaa ttgcttgca ggcaggaga agactagcc 
      accagaccg gcagctcac cactaa
    mRNA sequence
    • >TC014345 RA
      agagagcgc ttataaaag gcagtttaa cagtagctt 
      gctggttac tcggggcct caatagcga aagttgccc 
      cgacggagt tgtgtggtg ttgttgggc atgtggcgt 
      tgtgtgtaa cgagtgaga ttccgggct ttgtagtga 
      catagtgtg cctagtgca gccaatcca cgcggcaat 
      attgccgcc ctcaatttt ttttaaacc taattttaa 
      cgtagttta gtgcaaaga ctgaaaatg gcacaaggc 
      actttgctc atacttgta tgcgtaacg accgtagca 
      actttcgct ttcccccat cctggagag gacgtaatc 
      acacaacct aaacctggg gaagagtac gttttcgtg 
      caaaccacg ggttcaggc gcccgtccc atagcacga 
      aaagacaag ctaacaagt gaggagcgt cctttcgaa 
      accgcccac aaacttcca ccatctctc acatacatc 
      ctgaacgaa aacgacgac gatgttaag actaagacc 
      aaacgagcg gccaatccg gccgaagcc ccggctccc 
      gcccccgct ggtgcgatc aaactagac gtgcaaaaa 
      cttttagaa aaatacaag accgaactc aagactagc 
      acgaccgaa gctccgaaa accacagcg agaacgacg 
      cggaggaga ggcaacaag aggacgaaa aaggagatt 
      aaggaagtt gaagtcact agtcctagc cccgttttg 
      acgactgga gccagcgcc cccaagaaa gtcgacgat 
      gatattcct ctagtggct agtgggagc gagaaacag 
      cgatcgcgg attcagatc aaaaaggca cccaatggg 
      caggagtac gaatacgag tacgtttat tattactac 
      gacgaagat gatgatgcg aaagtgacc aatgcccat 
      gatgggcct gctaaggct gcgaacaag agtcgaggg 
      gctgtggag cgaaccagt gcagctcct gaagccaac 
      gaagttgtg ccctcgtcc cgcggcaag actagaggc 
      cgccaactg ggcgaggaa gaagtcagc gaggaaagg 
      cttcctgct aatacgagg tttcctcca agaagtcgc 
      aatctgaac actacgcct ttaccggaa gaggaaact 
      aaagcgaca agaggtcgg ggaagagga aggccaact 
      actgaggct gcacctagc actgatagt gtttcggac 
      gaaactcag gggtctcgg gggcgcacg cgtgccaac 
      gtgagaagg ccttccttg gaattggtt gacaatgcc 
      tcctttaac acccatcca ggatccgaa ccttccgga 
      agcagcccg tccttcccg acagacctt ccagctgga 
      ccggtacgt ttcttggga gcaacgcct aatgaaagc 
      gtggatcca cctgaaccc atcgccacg tccgacaat 
      cccccagaa ccaaagaaa gaagaaacc tcgactgaa 
      accgccgaa gaaaccggg acaacagcc atgtcagcg 
      atggacaaa gtggccctc gacctctat gccatcctc 
      caagggact cagaaactc tccggagaa gagggcgat 
      ttcagtagc gaggaaaat gcaactgat accgaggtc 
      acaactttg gaagccacg accgaacaa attattgag 
      actacaact cccacaact acgacaaca accaccaca 
      accacaaca ccagctcct acgaccaca accacgacg 
      gaagcacca atcggacga ggaagattt aagggacgt 
      gggaggaaa actacgcat tctgcgtcg acgaccgaa 
      gctgcacat gcagaaaca aaaccaaaa agtaaattc 
      agtagaccc agctttgga gggagacac agagctggg 
      gctaggtcg accacggag gcggcagtt gaacatgac 
      gcttccaag gaggaatcc aagccagtg agccactct 
      cggcccact ttaagtcga aatcgcttc ggcgcgacg 
      cgctccagg acccgaaca actgctgca ccttctgac 
      tccaacgac gactctcct agcagcacc tcagcccca 
      gtatcaaga ccatctctc acacgccct cgctcatcc 
      ttcaatctt cgcactaga ggacgttcc accaccaca 
      caatcaccc gaaaatgaa tctcacgac gaggaaact 
      gcttccagc tccgaacct gcgagcacc accgccaga 
      agtagactc gttcaaggc agtggcgta agaccccta 
      agacctgga cctcgaata aatctagcc agaagccgc 
      cctggacaa agtacaacc accacggaa gctcccgcc 
      gaagaccat gcaacggga gatgcggaa caaccaact 
      aacacgaac tctgaagct tcggaagaa aaagataaa 
      gaaacttcg gctccagta gataatagt cctttaggt 
      agattgaaa aacaagcac cgggtgaac gtgcaagcc 
      cggcccaaa gctgcagcg agcactccg gtgcaagtc 
      cgtcgtgtg aatccactg cttgcaagg aggagacct 
      gggcaaact acggaagct gcgtctagt gaagcacca 
      caagaagtg agcaccgaa gcagctgaa gctgaagaa 
      cacgaagaa accgaagct gcagttgaa gcgtccagt 
      actaccacc gaggaacct agaggatta aacaaattg 
      cttgcaggc aggagaaga ctagccacc agaccggca 
      gctcaccac taaggctga agaataatt gaaaatcgt 
      gaatttgaa ccagcaaac ttttttgat tttcagttg 
      agttagagt aattttaat aaaaaggaa taaagttat 
      ttattattt tcaagtttt tgagcaaaa ttttacatg 
      tactagctt ttgaatttt ccttaaact agattcgta 
      aaaactgtt ttgtagtct atgaaataa aacattgcg 
      agtattgtc tattatttt caataaaag tataatttt 
      gtacatacg taattacat actttattt atctcttac 
      gtatctagc ataatttcg agcttaagg atattttat 
      acttaatta ttagaaagg taattaaga cacttttat 
      acgcaggcc caaactgtg ccaatatta ttttacaat 
      tcaataaat gtaggtaat ttattttga ataatttac 
      ccaatgcaa aaattatta taacaatta ttttaaaat 
      aactatcat ttttataaa ccaatactt ctccaaaaa 
      ataaaagaa tccgctcat tcctttttc tgcaccgat 
      aatccttat acaatataa atctttgtg taaactgac 
      tgaaaacaa gttttctga aaaaattcg agtttttgc 
      tttttttaa tgttgactt agacatgtg atcattaaa 
      ggttgtaat ccttttgaa ataaagctc tattgtttg 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY