Status
Please wait ...
Gene information
General information
TC014337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9314722..9316513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014337
    iBeetle screen iB_09091

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014337 RA
      MSGNVNICA VTDDVKQAL KDFRFRKAQ 
      DTAALILKV DREKQQIII DEKLENITI 
      EELQESLQS HQPRYVILS YRQEHKDGR 
      VSYPLCFIY FTPRDSHAE LQIMYAGSK 
      ISLQREADL ARAFEIREL EDLTEEWLL 
      EKLGN
    CDS sequence
    • >TC014337 RA
      atgtcagga aacgtgaat atatgtgcc gtcactgat 
      gatgtgaaa caagctctt aaagacttc cgcttcaga 
      aaagcccaa gacactgcc gctttaatt ttgaaagtt 
      gatcgtgaa aaacagcaa ataataatc gacgaaaaa 
      ctggagaat atcacaatt gaagaactt caagagagt 
      ttacaaagc catcagccc cgttatgtc attttatca 
      tatcgccag gaacacaag gatggacgc gtttcttat 
      ccactgtgt ttcatctat ttcacacca cgcgacagc 
      cacgctgag ttgcaaata atgtatgca ggatcaaaa 
      atctccctg caacgtgaa gccgacctt gccagagct 
      tttgaaatt agagaactg gaggatttg acagaggaa 
      tggctcttg gaaaagtta gggaattaa
    mRNA sequence
    • >TC014337 RA
      atgcgtcaa agtgaaatg tcaaataag ttttgataa 
      aaacaagaa ctattttgg ataatttca gagactttt 
      tattgtgat ttagttcta gggttgcgg cgtagtatc 
      aaagaaatc aaaaatcca atagctcac gttaatgta 
      gctttgagg caaatcacg aaagaatgt caggaaacg 
      tgaatatat gtgccgtca ctgatgatg tgaaacaag 
      ctcttaaag acttccgct tcagaaaag cccaagaca 
      ctgccgctt taattttga aagttgatc gtgaaaaac 
      agcaaataa taatcgacg aaaaactgg agaatatca 
      caattgaag aacttcaag agagtttac aaagccatc 
      agccccgtt atgtcattt tatcatatc gccaggaac 
      acaaggatg gacgcgttt cttatccac tgtgtttca 
      tctatttca caccacgcg acagccacg ctgagttgc 
      aaataatgt atgcaggat caaaaatct ccctgcaac 
      gtgaagccg accttgcca gagcttttg aaattagag 
      aactggagg atttgacag aggaatggc tcttggaaa 
      agttaggga attaaatca aaccaaata tcatgcttt 
      cgtttatta aatttttaa tcaaattag tcctgattg 
      tgaaattac gttgtcatc ccaggtcac tttgatcac 
      aagtttaag aatcgaagg tgcggtgtt gcttattta 
      tgtttaatg attttagtt agaaatatt ccttaagca 
      ataaggatt acgtttatt tagtcagtt tagcagttg 
      ggacttttt gaaacgaca ttccaaaaa caccaaatt 
      gtgcgagaa aatctatat tctgttaaa ttctgaatg 
      ttgaatttt gtattgcga gtttatact tttgtaaca 
      aaataaaca aagtcaatg ataaatttt tatttacat 
      aataccttg aaataatgt aaagtacta gcaatgtat 
      gtaattatg atgtaaatc ttttctgat tagtatatt 
      gccaagaga acgtggtca agagctcca aaaccattt 
      catgtcaaa tgatgtgcc aagtttaat tttataaca 
      tcaagacac ccatttatg aaattttca cagcactct 
      cgacctcgt ttagactcc tggcatatt tttcagaaa 
      agactaaca ggatcgtgt acaaataat ctaaataaa 
      tcacattaa ctgccctaa gattaaagg aagtcagtt 
      ctttctatt atgcttaat attacgttc ctcaattac 
      taaaacata ataataaga accgaaatc ctttttaat 
      cccaccctc aattttttc ttctcaatt ctttggaag 
      ttgcaagta ctcacgccg agattaaac agtaaatca 
      ttctgcaac aaacagata gttaaggat ttcggttct 
      gatttaaaa ataatgtta gtttcaaaa tctagcaca 
      agatcacac cacttctat ataaacaca gccatatga 
      acagtacgt taataatta ttgccaact tgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY