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Gene information
General information
TC014313 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8956411..8958135
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014313
    iBeetle screen iB_09089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014313 RA
      MGGLNRNLF LAIVAAAVG SGFQHGYHT GVINVPQAI 
      LKDWIRTLW EDRLNSDLS DSTINVLWG VTTSIMNVG 
      GTIGGVLSG LAATKFGPK SALFYNNFL VLIASALMC 
      FAQLANSYA MMILGRLVI GVNCGFNAG LCPMYLTEI 
      SPVELRGAL GSVYQLLIV ISILTSQIV GLEMILGND 
      YWPVLFALS VVFAIFQVV TIFFCPESP KYLISVKQD 
      NQSAEKSLK FLRRSDDVN DELSALKKE DEANKNMPK 
      VTIKQMFRD KSLKIPLFI AMLVMVAQQ FSGINIVIF 
      YSTETFIRG GLSEENAQY ATIGIGIVN LIMTVISMI 
      LVEIAGRKT LLLVAFGGM AIDTLLLAL ALHFSKENS 
      SIPWVCIAL IAIYIILFA AGAGSIPWF LVTEIFNQE 
      ARPTAVSLA VPVNWIANF IVTLTFPPI AGLIGPFVF 
      LIFVVLNVL FFIFILKMV PETKNKTVE EIVSQWKE
    CDS sequence
    • >TC014313 RA
      atgggcggt cttaatcga aatctgttc ttggccatc 
      gttgcggca gcggtcggt tccggcttc cagcacggg 
      tatcacact ggggtgatc aacgtcccg caggccatt 
      ttgaaagat tggataaga acactatgg gaggaccga 
      ctcaactcg gatttgtcc gactcgaca ataaacgtg 
      ctgtgggga gtcaccacg tccatcatg aacgtcggg 
      ggaaccatc ggtggggtc ttaagtgga ctggccgcg 
      accaagttc ggccccaag tccgctttg ttctacaac 
      aactttctg gtgctcatt gcgtcagct ctgatgtgt 
      ttcgcacag ttggcaaac tcgtacgcg atgatgatc 
      ctggggaga ttggtcatc ggggtgaat tgtggcttc 
      aatgccggg ctttgcccc atgtacctt acggagatt 
      tcgccagtg gaactccga ggcgccctt ggcagtgtc 
      tatcaacta cttattgta atttccatc cttacttcc 
      caaattgtg gggcttgaa atgatccta ggcaatgac 
      tattggccc gtgcttttt gcattatcc gtcgtgttc 
      gcaattttt caggtcgtt acaatcttc ttttgcccg 
      gaatctccc aaatatttg atcagcgtt aagcaggat 
      aatcagtca gccgagaag tcgctaaaa ttcttgcga 
      cgctccgat gatgtcaat gacgaactt tccgcactt 
      aagaaggaa gacgaagcg aataaaaat atgccgaaa 
      gtgaccatc aaacaaatg ttccgagat aaatcattg 
      aaaattccc ttatttatt gctatgctt gtgatggtg 
      gcacaacaa ttcagtgga atcaacatt gttattttc 
      tactcaacg gaaaccttt attcgcggt ggtctctca 
      gaagagaat gcacaatac gccacaatt ggaattggt 
      atcgtcaat ctaatcatg acagtgatt tccatgatt 
      ttggtggaa atcgccgga aggaagact cttctcttg 
      gtggcgttt ggcgggatg gcaattgat actcttttg 
      ctggccttg gcattgcat ttctcgaaa gaaaattcg 
      tcgattcct tgggtctgt atcgctttg atagccatc 
      tacattatt ctttttgct gccggtgct ggttccatt 
      ccttggttt ctggtcacg gaaattttc aatcaggaa 
      gcgagacct acggctgtt tctctcgca gtgccagtc 
      aattggatt gctaacttt attgtcact ctaacgttt 
      cctccgatt gcggggctt attggacct ttcgtgttt 
      cttattttt gtcgtcttg aatgtactc ttctttatt 
      ttcatcctc aagatggtg ccagaaacc aagaacaag 
      acggtggaa gaaattgtg agccagtgg aaagagtag
    mRNA sequence
    • >TC014313 RA
      agtgaccaa aactgccaa aaggaatct attacaaat 
      tgacatttt aattttaca tttgatccg gttcgtagt 
      gaaaaaatc gaactgtca caatttttt ttgaaacat 
      gggcggtct taatcgaaa tctgttctt ggccatcgt 
      tgcggcagc ggtcggttc cggcttcca gcacgggta 
      tcacactgg ggtgatcaa cgtcccgca ggccatttt 
      gaaagattg gataagaac actatggga ggaccgact 
      caactcgga tttgtccga ctcgacaat aaacgtgct 
      gtggggagt caccacgtc catcatgaa cgtcggggg 
      aaccatcgg tggggtctt aagtggact ggccgcgac 
      caagttcgg ccccaagtc cgctttgtt ctacaacaa 
      ctttctggt gctcattgc gtcagctct gatgtgttt 
      cgcacagtt ggcaaactc gtacgcgat gatgatcct 
      ggggagatt ggtcatcgg ggtgaattg tggcttcaa 
      tgccgggct ttgccccat gtaccttac ggagatttc 
      gccagtgga actccgagg cgcccttgg cagtgtcta 
      tcaactact tattgtaat ttccatcct tacttccca 
      aattgtggg gcttgaaat gatcctagg caatgacta 
      ttggcccgt gctttttgc attatccgt cgtgttcgc 
      aatttttca ggtcgttac aatcttctt ttgcccgga 
      atctcccaa atatttgat cagcgttaa gcaggataa 
      tcagtcagc cgagaagtc gctaaaatt cttgcgacg 
      ctccgatga tgtcaatga cgaactttc cgcacttaa 
      gaaggaaga cgaagcgaa taaaaatat gccgaaagt 
      gaccatcaa acaaatgtt ccgagataa atcattgaa 
      aattccctt atttattgc tatgcttgt gatggtggc 
      acaacaatt cagtggaat caacattgt tattttcta 
      ctcaacgga aacctttat tcgcggtgg tctctcaga 
      agagaatgc acaatacgc cacaattgg aattggtat 
      cgtcaatct aatcatgac agtgatttc catgatttt 
      ggtggaaat cgccggaag gaagactct tctcttggt 
      ggcgtttgg cgggatggc aattgatac tcttttgct 
      ggccttggc attgcattt ctcgaaaga aaattcgtc 
      gattccttg ggtctgtat cgctttgat agccatcta 
      cattattct ttttgctgc cggtgctgg ttccattcc 
      ttggtttct ggtcacgga aattttcaa tcaggaagc 
      gagacctac ggctgtttc tctcgcagt gccagtcaa 
      ttggattgc taactttat tgtcactct aacgtttcc 
      tccgattgc ggggcttat tggaccttt cgtgtttct 
      tatttttgt cgtcttgaa tgtactctt ctttatttt 
      catcctcaa gatggtgcc agaaaccaa gaacaagac 
      ggtggaaga aattgtgag ccagtggaa agagtagaa 
      gattataat gttttggaa ataaacttg aaatgtatt 
      t
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