Status
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Gene information
General information
TC014312 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8945381..8947669
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014312
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09088

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014312 RA
      MAQPLCTLC ECPVETLHF DLRQSVSND TRTPLLMLL 
QDCAEEEFC ITNYFVCEM CYRLLNELD SIKVRARNI 
ENKFKQYLR KHCPNKNYI EREASNKSE ECTMCGQKI 
PGASFKDHL KTHDLETGF NIDDLSSVS EIQSTLDLF 
TKEAGSESV KKKQKDGTD PKPYKCTQC SKTWKTPTE 
LKNHMSAHS KERPYICEI CGQAYKRQQ ALTIHVGMH 
KGINPFTCV YCNKSFTQK GALMRHTPI HTGELPFQC 
DTCGKRFVH HTSFNIHKI THTGQKPYK CDVCGFLLI 
SASHLKRHM RVHTGEKKF SCGTCGKKF AEKYNLAVH 
EKLHLGVSK KKTYQCQIC DNVYNGQMK LEEHLLSVH 
HKVHPTDPL SLP
    CDS sequence
    • >TC014312 RA
      atggctcaa ccactgtgc acgttgtgc gagtgtcct 
gtcgaaact ctgcacttc gatctccga caatcggtc 
tccaacgat acaagaacc cctctgttg atgttgctc 
caagactgc gctgaggaa gagttttgc ataacaaat 
tatttcgtt tgcgagatg tgttatcgc ctcctgaat 
gaactagac agtattaaa gttagagca aggaacatc 
gaaaacaag tttaaacaa tatttgcgt aaacactgc 
cccaacaaa aactacatt gaacgagaa gcgagcaat 
aaaagcgag gaatgcaca atgtgtggt caaaaaatc 
cccggtgcg agtttcaag gatcacctg aagacgcat 
gatttggag acaggtttt aatattgat gatctcagt 
tcggtgtct gagattcag tccacgctg gatttgttt 
acgaaagaa gcgggaagt gaaagtgtg aagaaaaaa 
cagaaggat gggacggat ccaaaaccc tataaatgt 
actcagtgt tccaaaacc tggaaaacg ccaacggaa 
ctgaaaaat cacatgtcg gcgcatagc aaggaacgt 
ccctacatt tgcgaaatt tgtggccag gcgtacaag 
cgccaacaa gctttaaca atccacgta gggatgcac 
aaagggatc aatcccttc acgtgtgtc tactgcaac 
aaatcattc acgcagaag ggggctttg atgaggcac 
acacccatc cacacaggg gaactaccg tttcaatgt 
gatacgtgt ggtaaaaga ttcgttcac cacacctcc 
ttcaatatt cataaaatc actcacact gggcaaaaa 
ccgtacaaa tgtgacgtc tgtggcttt ttgctgata 
tcagcgtca caccttaaa agacacatg agggtgcat 
actggcgag aagaaattt tcttgtggc acttgtggg 
aaaaagttt gcggaaaag tacaacttg gctgttcat 
gaaaaactg catttgggg gtttccaag aagaaaaca 
taccagtgc cagatttgt gataatgtg tacaacggt 
caaatgaaa ctagaggag catctgctc agtgtccac 
cacaaggtg cacccaaca gacccatta tcattacca 
taa
    mRNA sequence
    • >TC014312 RA
      agtaattga aatggcatt aaaataacg caaattggg 
cttaatacg tgctggtta ttatttctt gacataacc 
aggaagcta ttcggagga tttaagtaa caaatattt 
ttttggggt gatgacagt tgacataaa caaaaacat 
aaccttaca agtgaatac atttttttc aaattaaaa 
ttggtggtg gtgtggtgc ttggtgtta agccgattt 
cttgaaatg gctcaacca ctgtgcacg ttgtgcgag 
tgtcctgtc gaaactctg cacttcgat ctccgacaa 
tcggtctcc aacgataca agaacccct ctgttgatg 
ttgctccaa gactgcgct gaggaagag ttttgcata 
acaaattat ttcgtttgc gagatgtgt tatcgcctc 
ctgaatgaa ctagacagt attaaagtt agagcaagg 
aacatcgaa aacaagttt aaacaatat ttgcgtaaa 
cactgcccc aacaaaaac tacattgaa cgagaagcg 
agcaataaa agcgaggaa tgcacaatg tgtggtcaa 
aaaatcccc ggtgcgagt ttcaaggat cacctgaag 
acgcatgat ttggagaca ggttttaat attgatgat 
ctcagttcg gtgtctgag attcagtcc acgctggat 
ttgtttacg aaagaagcg ggaagtgaa agtgtgaag 
aaaaaacag aaggatggg acggatcca aaaccctat 
aaatgtact cagtgttcc aaaacctgg aaaacgcca 
acggaactg aaaaatcac atgtcggcg catagcaag 
gaacgtccc tacatttgc gaaatttgt ggccaggcg 
tacaagcgc caacaagct ttaacaatc cacgtaggg 
atgcacaaa gggatcaat cccttcacg tgtgtctac 
tgcaacaaa tcattcacg cagaagggg gctttgatg 
aggcacaca cccatccac acaggggaa ctaccgttt 
caatgtgat acgtgtggt aaaagattc gttcaccac 
acctccttc aatattcat aaaatcact cacactggg 
caaaaaccg tacaaatgt gacgtctgt ggctttttg 
ctgatatca gcgtcacac cttaaaaga cacatgagg 
gtgcatact ggcgagaag aaattttct tgtggcact 
tgtgggaaa aagtttgcg gaaaagtac aacttggct 
gttcatgaa aaactgcat ttgggggtt tccaagaag 
aaaacatac cagtgccag atttgtgat aatgtgtac 
aacggtcaa atgaaacta gaggagcat ctgctcagt 
gtccaccac aaggtgcac ccaacagac ccattatca 
ttaccataa cttgttatc acttggcaa gagaatagt 
gaattgttt gaacgtgtt ctaataaat tgttaattt 
ttacgaaaa cattttatt caaagcctg ctcattcag 
tctaaaaag ctgccattt ctcggaaac tgttaaatt 
ttcagcgcc caaactttt ataaaagtc tatggcagg 
gttccagga gagaacgtg aaaatctaa tcttttgct 
agataattc atgtgcggt tttggccac tgccataaa 
taactgttt ccctattcc tattttcct gtatgcggg 
ttggacgta taaatcttc aaggaaaac tgattttcc 
caaccaagt cgagtaaga cgtgtaata caatgcata 
tccgactaa gtgtccatc tgagatttc tgccacaag 
acacgtgaa aactggatt atcggtctc aaacccgtc 
tttaatcaa ggttttttc gtcaatttt tacttggtc 
ttctagttt ttcaaattc tgcaagttc ctgaaaaat 
gcgatatcg cattgccag gggttaaaa attaattaa 
ataatacct tgattagtt tgtagactt ggatcatat 
cctcctttt tggcttttc ttatagtta cattaatct 
cagaacagt ccagcatgt ttgagagat gaagtgaca 
cttttcaag caaatgtgt gttaacaga gataaggta 
tgacgtctg gaatccaca aatgtaatt gagacaatt 
agatacaat tttattgag atataaata gtactacag 
attttgtga attcatgct gtaactgac cattaatcg 
aaaaaagta atttaataa gaatattac aattagtta 
ctttcacct aacaatatt aataaaaaa aattgagaa 
at

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY