Status
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Gene information
General information
TC014311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8935001..8936311
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014311
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09087

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014311 RA
      MALALSTTV KLLEDKLLS LTISCMSVR ERHKLNPPP 
      KLRNPTWFT QKNRVSFTD PLTTENQEF LKEVVQDQC 
      NNISSEKPK DLDVVWRPG LQRTGVIAR KIGIYPMWL 
      KNGKKISTT LLQVLDNQV IKYYTPESY NPPRPRAGR 
      VSNRKGCLL LGAEQGDPS EFTREYCGI FKDSGVIPK 
      KTLARFFVS PDAALPPGT SISALHFCV GNYVDVRGL 
      TIDRGFQGV MKRHGFKGM PASHGVTKT HRRGGNIGG 
      GGEKGRVWP GTKMPGHMG NRYRILRAL KIWRINTKY 
      NVLYVSGQN IPGATNSIV YIYDTKVVT KRHTEPVPF 
      PTYWGGEEV PENIYDKEL HEFGEPSIV FKEQ
    CDS sequence
    • >TC014311 RA
      atggctctt gcattgtct accaccgtt aagttgtta 
      gaggacaag ttgctctcg ttgaccatt tcttgcatg 
      tctgtgagg gagcgacat aaattgaac cctccgcca 
      aaactacga aacccgact tggtttact caaaaaaat 
      cgagtctcg ttcactgac cccctcacc accgaaaac 
      caagagttc ctgaaagag gtggttcaa gaccaatgc 
      aacaatatc tcaagtgaa aaacccaaa gatttagat 
      gtagtctgg cgtccgggt ctgcaaagg accggcgtg 
      atagcgcgc aaaatcggg atttatccc atgtggtta 
      aaaaacggt aaaaaaatc tcgaccaca ttattacaa 
      gttttggac aaccaagtc attaaatat tacacgcct 
      gaaagttac aatcctccc cgaccacgt gctgggcgg 
      gtttccaac agaaagggg tgcctccta ctgggggct 
      gaacaaggg gacccgtca gagttcacg cgggagtat 
      tgtggaatt tttaaagac tcgggggtt attccgaaa 
      aaaacatta gctcggttt tttgtcagc cctgatgcg 
      gctctacct ccaggcacc tcaattagc gctctccac 
      ttctgcgtt ggtaattat gtggacgtc cgggggctc 
      acaatcgac cgcggcttc cagggtgtg atgaaacgc 
      cacggtttc aaaggaatg ccggcatcc cacggtgtg 
      acgaaaact caccgccgt gggggtaac attggaggt 
      ggtggagag aaaggtcgc gtgtggcct ggtactaag 
      atgccaggc catatgggc aaccggtat cgcatactt 
      agagcactc aaaatttgg aggattaat accaagtat 
      aatgtcttg tacgtgtct ggacagaat attccagga 
      gcaacaaac tcaattgtg tatatttat gatacaaaa 
      gttgttact aaacgacac acggaacca gtaccgttt 
      cccacttac tggggagga gaggaggtg cccgaaaat 
      atctacgat aaggaattg cacgaattt ggagaacct 
      tcaattgtt ttcaaagaa cagtaa
    mRNA sequence
    • >TC014311 RA
      gttctaacc tcaaaaatg ttcttttaa gtgtaatta 
      ttttaataa aatggctct tgcattgtc taccaccgt 
      taagttgtt agaggacaa gttgctctc gttgaccat 
      ttcttgcat gtctgtgag ggagcgaca taaattgaa 
      ccctccgcc aaaactacg aaacccgac ttggtttac 
      tcaaaaaaa tcgagtctc gttcactga ccccctcac 
      caccgaaaa ccaagagtt cctgaaaga ggtggttca 
      agaccaatg caacaatat ctcaagtga aaaacccaa 
      agatttaga tgtagtctg gcgtccggg tctgcaaag 
      gaccggcgt gatagcgcg caaaatcgg gatttatcc 
      catgtggtt aaaaaacgg taaaaaaat ctcgaccac 
      attattaca agttttgga caaccaagt cattaaata 
      ttacacgcc tgaaagtta caatcctcc ccgaccacg 
      tgctgggcg ggtttccaa cagaaaggg gtgcctcct 
      actgggggc tgaacaagg ggacccgtc agagttcac 
      gcgggagta ttgtggaat ttttaaaga ctcgggggt 
      tattccgaa aaaaacatt agctcggtt ttttgtcag 
      ccctgatgc ggctctacc tccaggcac ctcaattag 
      cgctctcca cttctgcgt tggtaatta tgtggacgt 
      ccgggggct cacaatcga ccgcggctt ccagggtgt 
      gatgaaacg ccacggttt caaaggaat gccggcatc 
      ccacggtgt gacgaaaac tcaccgccg tgggggtaa 
      cattggagg tggtggaga gaaaggtcg cgtgtggcc 
      tggtactaa gatgccagg ccatatggg caaccggta 
      tcgcatact tagagcact caaaatttg gaggattaa 
      taccaagta taatgtctt gtacgtgtc tggacagaa 
      tattccagg agcaacaaa ctcaattgt gtatattta 
      tgatacaaa agttgttac taaacgaca cacggaacc 
      agtaccgtt tcccactta ctggggagg agaggaggt 
      gcccgaaaa tatctacga taaggaatt gcacgaatt 
      tggagaacc ttcaattgt tttcaaaga acagtaata 
      ataaatctt gtagattgt cttagtttt tgaaaaaat 
      aaagattaa gttgatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY