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Gene information
General information
TC013503 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8718146..8722365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013503
    iBeetle screen iB_09086

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013503 RA
      MITNFLGSR LLPGYPDLR TSLLHQKLQ MLNCCIERK 
      QARENTTTT FDSAEGSST DEDEFFDCA DKPEEETRK 
      KEKYSLWNQ PVGRLGKFN NLKLLKTGD PLYIPVTQE 
      PVPKSEDQL EEDTDVLLK LGSDAQGSE LRAKMMSAS 
      LLSDMESFK AANPGSVLE DFIRWYSPR DWIESEELD 
      EWGQKKGEL SSRMMIEDN FWVQTWDSA KPVPAHRQK 
      RLFDDTREA EKVLHFLDS RSLCQICEM LIPVLCHIA 
      IYRVGEERP RELADAGER LRALVKTAE RISRESKLQ 
      PRRFEGFIQ EVTALELRT SQVNSLMYK FNPAGAVDE 
      NLSNLIGKL VSGREVEIA DRVDSTLGN RIVGMFSDA 
      QKAANMILT EQATEDGKS VKGNAAFPQ ASEREIVMR 
      VSVPRPATY SAECPQFLR AVLGKSEFR LVGAFSEDI 
      VFF
    CDS sequence
    • >TC013503 RA
      atgatcaca aattttctt ggttccaga cttttgccc 
      ggctacccc gacttacgc accagcctc ttacaccaa 
      aaactccaa atgctgaac tgctgcatt gagcgaaaa 
      caggcccgc gagaacacc accaccacc ttcgattcg 
      gccgaaggc tcctccacc gacgaggac gagttcttc 
      gactgcgcg gacaaaccg gaggaagag actcggaaa 
      aaagaaaaa tactccctg tggaaccaa cccgtgggc 
      cgcctcggg aagtttaac aatttgaaa ttactcaaa 
      accggcgac cccctctac atccccgtc acccaggag 
      cccgtcccc aagagcgag gaccagctg gaggaggac 
      accgacgtc ctgctcaag ctgggctcc gacgcccag 
      ggctccgag ctccgggcc aaaatgatg agcgcttcg 
      ctcctctcc gacatggag tccttcaaa gcggcgaat 
      cccggatcg gtcctggag gacttcatc cggtggtac 
      tccccgcgg gactggatc gagagcgag gagttggac 
      gaatggggg cagaagaag ggcgaattg agctcgcgc 
      atgatgatc gaggataac ttctgggtc cagacgtgg 
      gactcggcc aagcccgtg ccggcgcac cgccagaag 
      cggctcttc gacgacacg cgggaggcg gagaaggtg 
      ctccatttc ctggactcc aggagcctg tgccagatc 
      tgcgagatg ctgatcccg gtcttgtgc cacatcgcc 
      atctacaga gtgggcgaa gagcggccg cgcgagctt 
      gcggacgct ggcgaaaga ttgcgcgcg ttggttaaa 
      accgccgag cggatttct agagagagc aagctgcag 
      ccgcgccgg ttcgagggc ttcatccaa gaggtgaca 
      gcactcgag ctgcgcacc tcgcaagtc aactcgctg 
      atgtataaa ttcaatccc gcgggagcc gttgatgag 
      aatttatct aatttgatc gggaagctg gttagcgga 
      cgcgaagtg gaaattgcc gatcgagtc gattccact 
      ttgggtaat cgaattgtt ggcatgttt tccgacgcg 
      cagaaagcg gctaatatg attcttacg gaacaagcg 
      accgaggat ggaaaaagc gtcaaggga aatgccgcg 
      tttcctcaa gccagtgag agggaaatt gttatgagg 
      gtctcggtg ccccgaccg gcgacctac tcggcggaa 
      tgcccgcag ttcctcaga gcggtcctg ggcaagagc 
      gagttccgg ctcgtgggg gccttctcc gaggacata 
      gtcttcttc taa
    mRNA sequence
    • >TC013503 RA
      gcagcatta ccacaaatt acttattta acatctttt 
      cgtagctat aattaaatt accggaggc tgcatttga 
      aacagtaca catttttga ttatcatca acttacttg 
      aaaatattc caataaccg gcaagaagt tgcactaat 
      tcatctaaa ccgaggact gatcattag caattttat 
      ttcgttaaa cttgtaaaa ctgcacatg cacgatagt 
      ctgaaagac caatgcact cacacaaca tcgacctac 
      cagttcttg tagctaact ggacgcgca accatggag 
      aagtcttga gctgttaac aaaattttc ttcaacgta 
      aacattata cgttcactt ttgcgtcgt ttaagtaga 
      ccgccgtgc ccagatgat cacaaattt tcttggttc 
      cagactttt gcccggcta ccccgactt acgcaccag 
      cctcttaca ccaaaaact ccaaatgct gaactgctg 
      cattgagcg aaaacaggc ccgcgagaa caccaccac 
      caccttcga ttcggccga aggctcctc caccgacga 
      ggacgagtt cttcgactg cgcggacaa accggagga 
      agagactcg gaaaaaaga aaaatactc cctgtggaa 
      ccaacccgt gggccgcct cgggaagtt taacaattt 
      gaaattact caaaaccgg cgaccccct ctacatccc 
      cgtcaccca ggagcccgt ccccaagag cgaggacca 
      gctggagga ggacaccga cgtcctgct caagctggg 
      ctccgacgc ccagggctc cgagctccg ggccaaaat 
      gatgagcgc ttcgctcct ctccgacat ggagtcctt 
      caaagcggc gaatcccgg atcggtcct ggaggactt 
      catccggtg gtactcccc gcgggactg gatcgagag 
      cgaggagtt ggacgaatg ggggcagaa gaagggcga 
      attgagctc gcgcatgat gatcgagga taacttctg 
      ggtccagac gtgggactc ggccaagcc cgtgccggc 
      gcaccgcca gaagcggct cttcgacga cacgcggga 
      ggcggagaa ggtgctcca tttcctgga ctccaggag 
      cctgtgcca gatctgcga gatgctgat cccggtctt 
      gtgccacat cgccatcta cagagtggg cgaagagcg 
      gccgcgcga gcttgcgga cgctggcga aagattgcg 
      cgcgttggt taaaaccgc cgagcggat ttctagaga 
      gagcaagct gcagccgcg ccggttcga gggcttcat 
      ccaagaggt gacagcact cgagctgcg cacctcgca 
      agtcaactc gctgatgta taaattcaa tcccgcggg 
      agccgttga tgagaattt atctaattt gatcgggaa 
      gctggttag cggacgcga agtggaaat tgccgatcg 
      agtcgattc cactttggg taatcgaat tgttggcat 
      gttttccga cgcgcagaa agcggctaa tatgattct 
      tacggaaca agcgaccga ggatggaaa aagcgtcaa 
      gggaaatgc cgcgtttcc tcaagccag tgagaggga 
      aattgttat gagggtctc ggtgccccg accggcgac 
      ctactcggc ggaatgccc gcagttcct cagagcggt 
      cctgggcaa gagcgagtt ccggctcgt gggggcctt 
      ctccgagga catagtctt cttctaatg taccatttt 
      attactact actgaataa acgattatt tatttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY