Status
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Gene information
General information
TC010446 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7420845..7422732
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010446
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09079

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010446 RA
      MHQNCADKP LEVNPENAS REITWYRRE LNKSPPVND 
      TTDCTFIIN GEEIRVHKG ILIWHSPVF KKMFSGEMA 
      SDTIGISDI AVEDFTQML EFLYRKTVV IKSVLHAWS 
      LFYIAHKYL LDCFASSCT RFIEENLDI TNLMLSYEY 
      AEMYGHVQL QKVCLEDIE SYIRGIFLE DYDYHMKPT 
      SLATILKRQ VLNVLNNNE LILGVLNWA VTECDLKNI 
      SLSPENIVG LLESLQVFE YLTNLDFNT DSSENDLIA 
      NCSRLIKLK STKPYRKYS QKRSSFHCK CRDTYKIAT 
      RVELRERVY LYSHINVNR DTLLYGLVL VTEDRPIND 
      MNNEYKGEI SVHISEYQN PVIICETRF CDIMRYEDL 
      VYVPLSNVI YLESDKIYT IRVEYKNFD RWYSGNKLG 
      SVVCNYMSS KLVDKKKKT IFLFRELTG SVLKGLSFY 
      PV
    CDS sequence
    • >TC010446 RA
      atgcatcaa aactgtgcc gataaacca cttgaggtg 
      aatcccgag aatgcgagc cgggaaatc acctggtat 
      cgcagggag ctaaacaaa tcgccgcca gttaacgac 
      accacagac tgcacgttc atcatcaat ggtgaagaa 
      attcgagtc cataaaggc atcctaatt tggcacagt 
      cccgttttc aagaaaatg ttttctggg gaaatggcg 
      tccgatacg attggtatc agtgatatc gccgttgag 
      gatttcacc caaatgttg gaatttctt tataggaaa 
      accgtggtc attaagtca gtattacac gcctggtca 
      ttgttttac attgctcat aagtatttg ttagattgt 
      tttgcaagt tcctgtact agatttata gaggaaaat 
      ctcgatatt acgaattta atgttaagt tatgaatat 
      gcggagatg tacggtcac gtgcagctc cagaaagtt 
      tgcttggaa gatatagaa agttacatt agaggaatt 
      tttttggaa gattacgat tatcacatg aaacccaca 
      agccttgcc acaatcttg aaaagacag gttttgaat 
      gttttaaac aataatgaa ctgattttg ggggtttta 
      aactgggct gttactgaa tgtgatttg aaaaatatt 
      agtttatcg cctgaaaat attgtaggt ttgttagaa 
      tcgttacaa gtttttgag tatttaacc aatctggat 
      tttaacact gattccagt gaaaacgat ctaattgcc 
      aattgttct cgtctaata aagttaaaa agtaccaaa 
      ccttataga aagtattca caaaaacgt tcaagtttc 
      cattgcaaa tgtcgagat acatacaaa atagccact 
      agagtagag ttaagagaa cgagtttat ttatattca 
      cacatcaac gtcaataga gacacttta ctctacggc 
      ttagtatta gtgacggaa gataggccc ataaatgat 
      atgaataat gagtacaag ggcgagatt tctgtacac 
      ataagtgaa tatcagaat cctgtgatt atttgtgaa 
      accagattt tgtgatatt atgcgctat gaggacctg 
      gtttatgtt cctttatcc aacgttatt tacttagaa 
      tctgataaa atttacacg attagagtc gagtataaa 
      aattttgat aggtggtat tctggcaac aaattgggc 
      tcagttgtg tgcaattac atgagcagt aaattagtt 
      gataagaaa aagaagact atttttttg ttcagagaa 
      ttgaccgga tctgtgtta aagggactc tccttttat 
      ccagtctaa
    mRNA sequence
    • >TC010446 RA
      cgaaatgtt tacaaaccc aaccacaac ctcacagtt 
      cttgagcag cctagcaat taactcgaa atagctgtt 
      ttaatagca ataaaaggg tgatttggc gttaaaatg 
      catcaaaac tgtgccgat aaaccactt gaggtgaat 
      cccgagaat gcgagccgg gaaatcacc tggtatcgc 
      agggagcta aacaaatcg ccgccagtt aacgacacc 
      acagactgc acgttcatc atcaatggt gaagaaatt 
      cgagtccat aaaggcatc ctaatttgg cacagtccc 
      gttttcaag aaaatgttt tctggggaa atggcgtcc 
      gatacgatt ggtatcagt gatatcgcc gttgaggat 
      ttcacccaa atgttggaa tttctttat aggaaaacc 
      gtggtcatt aagtcagta ttacacgcc tggtcattg 
      ttttacatt gctcataag tatttgtta gattgtttt 
      gcaagttcc tgtactaga tttatagag gaaaatctc 
      gatattacg aatttaatg ttaagttat gaatatgcg 
      gagatgtac ggtcacgtg cagctccag aaagtttgc 
      ttggaagat atagaaagt tacattaga ggaattttt 
      ttggaagat tacgattat cacatgaaa cccacaagc 
      cttgccaca atcttgaaa agacaggtt ttgaatgtt 
      ttaaacaat aatgaactg attttgggg gttttaaac 
      tgggctgtt actgaatgt gatttgaaa aatattagt 
      ttatcgcct gaaaatatt gtaggtttg ttagaatcg 
      ttacaagtt tttgagtat ttaaccaat ctggatttt 
      aacactgat tccagtgaa aacgatcta attgccaat 
      tgttctcgt ctaataaag ttaaaaagt accaaacct 
      tatagaaag tattcacaa aaacgttca agtttccat 
      tgcaaatgt cgagataca tacaaaata gccactaga 
      gtagagtta agagaacga gtttattta tattcacac 
      atcaacgtc aatagagac actttactc tacggctta 
      gtattagtg acggaagat aggcccata aatgatatg 
      aataatgag tacaagggc gagatttct gtacacata 
      agtgaatat cagaatcct gtgattatt tgtgaaacc 
      agattttgt gatattatg cgctatgag gacctggtt 
      tatgttcct ttatccaac gttatttac ttagaatct 
      gataaaatt tacacgatt agagtcgag tataaaaat 
      tttgatagg tggtattct ggcaacaaa ttgggctca 
      gttgtgtgc aattacatg agcagtaaa ttagttgat 
      aagaaaaag aagactatt tttttgttc agagaattg 
      accggatct gtgttaaag ggactctcc ttttatcca 
      gtctaaaat taaattgtg ataaaatca caaaacaat 
      aaactctaa ctttattta aattgtctg aatagtgtc 
      ttccacctg ttcggttaa tatttacaa taaaaataa 
      aaaaacagt attatagtg ttttttcgt ctgtttatt 
      agacacaca aaatttatt ataaacaac aaaaacact 
      aagttggtt tatttttgt gaagctcaa atagaacca 
      gaagacctg aatttattt tgttaataa aaagaagtt 
      aatcacgat ttacattta actttttag aatttttgt 
      ttattgaga cccatttaa ttgatgttt gataaatgt 
      tataaatta ttattagtg atataagtg atataaaag 
      catctgttt atttagggg attgtttta aggggcact 
      tttgttttg gttaagttg gaataaaag ctttaataa 
      att
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY