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Gene information
General information
TC010429 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7335306..7338473
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010429
    iBeetle screen iB_09078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentiallynot present - Number of animals showing the phenotype: 4

    mature egg not present - Number of animals showing the phenotype: 3

    yolk potentially decreased - Number of animals showing the phenotype: 4

    Comment:
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010429 RA
      MENLRNRKH KDKLIDQYS DNEKKSAQK EEIQVEKCK 
      KRNGNTPSY LNNDEKEVS YSVKRAKFL RINFEIDLI 
      SVGLFLCGL LTRMYRLEE PKNIVFDEL HYGKYVSLY 
      MKNTFFFDS HPPLGKQLI AAVAYLAGF DGKFKFDKI 
      GSPYPDTIP LFALRFVPA FFGSLIIPT SYHLMLELG 
      LTQWTSLLA AVLLLLDNA LLTQSRFIL MESMLLFFA 
      LFGIFCILK FRQYYSNPY CIFWFLWLG LGFTSLTCA 
      LSVKYVGFY SFCLGVAIL GRDFWKMLS SSTLTDRAL 
      LVRFVAQTA VAASVSFLI YLAVFYIHL KVLYKAGPH 
      DSIMTSAFQ ASLEGGLAS ITKGQPLLV AHGSQITLR 
      HTHGRTCWL HSHNHVYPI RYPDKRGSS HQQQVTCYS 
      FKDVNNWWI VKRPNKNDL VVEQPIDAI KHGDVIQLV 
      HGITSRALN SHDVAAPVS PQCQEVSCY IDYNVSMPA 
      QNLWRVEIL NRDQNGDSW HTIQSLIRL IHVDTNTAL 
      KFTGRQLPD WGFHQHEVA ADRIINQDD TVWNVEEHR 
      YTKSDDQKE RERELVTAE MIPTAATSL TFWEKFFEL 
      HYKMLFSGT ESVQNHMYS SEPLEWPLM ARGIAYWVS 
      STSNAQIHL IGNLLLWYS GSAGLATYS TLLVFYLLR 
      RRRQCYDLD TKKWEQFQL IGELFFTGY LFHYLPYFF 
      VERTLFLHH YLPAFTFKT LLLAALLEH VYSTISDIF 
      KIRVIKYIF ILLIFLWIC CIFYVFMKF IVLCYGTTP 
      LTTNDIIDL RWKDTWDFI VHKN
    CDS sequence
    • >TC010429 RA
      atggaaaat ttacgcaac aggaaacat aaagataag 
      ttgattgat caatacagc gacaatgag aaaaaaagc 
      gcacaaaaa gaggaaatt caagtcgaa aaatgtaag 
      aaacgaaac ggcaacact ccttcctac ttaaacaat 
      gacgaaaaa gaggtctca tactcggtt aaaagggcg 
      aagttcctg cgaatcaac ttcgaaatt gatttaatc 
      tcagtgggg ttatttttg tgtggttta ctgactcga 
      atgtacagg ctggaagaa ccgaaaaat attgtattt 
      gatgagtta cattatgga aagtacgtc tctctctac 
      atgaaaaac acgtttttc tttgattct cacccccca 
      ctgggcaag cagttgatc gcagcggtg gcctacttg 
      gccggtttc gacggaaaa ttcaaattc gataaaatc 
      gggagccca taccccgac acaattccg ttatttgcg 
      ctacgtttc gtccctgcg tttttcggc agtttgata 
      atacccacc tcttatcac ttgatgctt gagctgggg 
      ttgacgcaa tggacttcg ttgctggcg gcagtatta 
      cttttacta gataacgct ttgttgacc caaagccgt 
      ttcattttg atggagtca atgctactc tttttcgcc 
      ttatttggc attttttgc attttgaaa tttcgccaa 
      tactactcc aacccctat tgcattttt tggtttttg 
      tggctgggg ttggggttt acttcactg acgtgcgct 
      ttgtccgtg aagtacgtc gggttttac tcgttttgt 
      ttgggggtt gccatcctc ggacgcgac ttttggaaa 
      atgttgtct tcctccacc ctcacagat agggccctt 
      ttggtgcgg tttgtggcc caaacggcc gttgccgct 
      tcagtttca tttttaatc tacttggct gttttttac 
      attcatttg aaagtattg tacaaagcg ggaccacat 
      gatagtatt atgaccagt gcgtttcaa gcctccttg 
      gagggtggc ttggcttcc atcaccaag ggacagccc 
      ctcttggtg gcacatggg tcgcagatt actctaagg 
      cacactcat gggaggacg tgctggtta cattcccac 
      aatcacgtt tatccaatc agatatccc gacaaaaga 
      ggttctagt catcaacag caagtcact tgttattca 
      ttcaaggat gttaataac tggtggatt gtgaagagg 
      ccaaataaa aacgatctt gtggttgaa caacccata 
      gatgcaatc aaacatgga gatgttatc cagctagta 
      catggtata acaagccgt gccttaaac tcccacgac 
      gtggcagcg cccgtgtcc ccccaatgc caggaagtg 
      tcctgttac atcgactac aacgtctca atgcccgcg 
      caaaacctc tggagggtg gaaatttta aaccgggat 
      caaaacggg gacagctgg cacacaata caatcactg 
      ataagactg atacacgtt gatacgaac acagctctt 
      aaattcacc ggacgacaa ctcccagat tggggcttc 
      catcagcat gaagttgcc gctgaccgg attatcaat 
      caagacgac acggtgtgg aacgtcgag gagcaccga 
      tacactaaa tccgacgat caaaaagaa cgcgaacgc 
      gaactcgtt accgccgaa atgatccca acagctgca 
      acttcgcta acattctgg gaaaaattc ttcgaattg 
      cattacaaa atgctgttt tcaggcacc gaaagtgtg 
      caaaatcat atgtatagt tcggagcct ctggaatgg 
      ccgttgatg gccaggggg atcgcctat tgggtctca 
      tcgacgagc aacgcgcaa atacatcta attggaaat 
      ctcttgctc tggtattcc ggaagtgcc ggccttgcc 
      acctactca actctactt gtcttctat cttcttcgt 
      cgacggaga cagtgttac gaccttgat acgaaaaaa 
      tgggaacaa tttcagttg attggagag ttatttttt 
      acaggctat ttgttccat tatctaccg tattttttc 
      gtggagcgg acgctcttt ctacaccac tatttgccc 
      gcatttacg ttcaaaacg ctgctatta gcggccctg 
      cttgaacac gtttattca acaattagt gatattttt 
      aaaattagg gtcataaag tatattttt atattactc 
      atattttta tggatttgt tgcatattt tacgttttt 
      atgaaattt atagtattg tgttatggt acaaccccg 
      ttaactaca aatgacatt attgatctg agatggaaa 
      gacacttgg gacttcata gttcataaa aattag
    mRNA sequence
    • >TC010429 RA
      aatctcaga agatcggtg ctaatgacc tcgaagtgc 
      tacgtggat gtttcacct gccttaatg acatatttt 
      ccaaaaatg tgtgtgcct ttgctgctt ctttgaaat 
      aaatggaaa atttacgca acaggaaac ataaagata 
      agttgattg atcaataca gcgacaatg agaaaaaaa 
      gcgcacaaa aagaggaaa ttcaagtcg aaaaatgta 
      agaaacgaa acggcaaca ctccttcct acttaaaca 
      atgacgaaa aagaggtct catactcgg ttaaaaggg 
      cgaagttcc tgcgaatca acttcgaaa ttgatttaa 
      tctcagtgg ggttatttt tgtgtggtt tactgactc 
      gaatgtaca ggctggaag aaccgaaaa atattgtat 
      ttgatgagt tacattatg gaaagtacg tctctctct 
      acatgaaaa acacgtttt tctttgatt ctcaccccc 
      cactgggca agcagttga tcgcagcgg tggcctact 
      tggccggtt tcgacggaa aattcaaat tcgataaaa 
      tcgggagcc cataccccg acacaattc cgttatttg 
      cgctacgtt tcgtccctg cgtttttcg gcagtttga 
      taataccca cctcttatc acttgatgc ttgagctgg 
      ggttgacgc aatggactt cgttgctgg cggcagtat 
      tacttttac tagataacg ctttgttga cccaaagcc 
      gtttcattt tgatggagt caatgctac tctttttcg 
      ccttatttg gcatttttt gcattttga aatttcgcc 
      aatactact ccaacccct attgcattt tttggtttt 
      tgtggctgg ggttggggt ttacttcac tgacgtgcg 
      ctttgtccg tgaagtacg tcgggtttt actcgtttt 
      gtttggggg ttgccatcc tcggacgcg acttttgga 
      aaatgttgt cttcctcca ccctcacag atagggccc 
      ttttggtgc ggtttgtgg cccaaacgg ccgttgccg 
      cttcagttt catttttaa tctacttgg ctgtttttt 
      acattcatt tgaaagtat tgtacaaag cgggaccac 
      atgatagta ttatgacca gtgcgtttc aagcctcct 
      tggagggtg gcttggctt ccatcacca agggacagc 
      ccctcttgg tggcacatg ggtcgcaga ttactctaa 
      ggcacactc atgggagga cgtgctggt tacattccc 
      acaatcacg tttatccaa tcagatatc ccgacaaaa 
      gaggttcta gtcatcaac agcaagtca cttgttatt 
      cattcaagg atgttaata actggtgga ttgtgaaga 
      ggccaaata aaaacgatc ttgtggttg aacaaccca 
      tagatgcaa tcaaacatg gagatgtta tccagctag 
      tacatggta taacaagcc gtgccttaa actcccacg 
      acgtggcag cgcccgtgt ccccccaat gccaggaag 
      tgtcctgtt acatcgact acaacgtct caatgcccg 
      cgcaaaacc tctggaggg tggaaattt taaaccggg 
      atcaaaacg gggacagct ggcacacaa tacaatcac 
      tgataagac tgatacacg ttgatacga acacagctc 
      ttaaattca ccggacgac aactcccag attggggct 
      tccatcagc atgaagttg ccgctgacc ggattatca 
      atcaagacg acacggtgt ggaacgtcg aggagcacc 
      gatacacta aatccgacg atcaaaaag aacgcgaac 
      gcgaactcg ttaccgccg aaatgatcc caacagctg 
      caacttcgc taacattct gggaaaaat tcttcgaat 
      tgcattaca aaatgctgt tttcaggca ccgaaagtg 
      tgcaaaatc atatgtata gttcggagc ctctggaat 
      ggccgttga tggccaggg ggatcgcct attgggtct 
      catcgacga gcaacgcgc aaatacatc taattggaa 
      atctcttgc tctggtatt ccggaagtg ccggccttg 
      ccacctact caactctac ttgtcttct atcttcttc 
      gtcgacgga gacagtgtt acgaccttg atacgaaaa 
      aatgggaac aatttcagt tgattggag agttatttt 
      ttacaggct atttgttcc attatctac cgtattttt 
      tcgtggagc ggacgctct ttctacacc actatttgc 
      ccgcattta cgttcaaaa cgctgctat tagcggccc 
      tgcttgaac acgtttatt caacaatta gtgatattt 
      ttaaaatta gggtcataa agtatattt ttatattac 
      tcatatttt tatggattt gttgcatat tttacgttt 
      ttatgaaat ttatagtat tgtgttatg gtacaaccc 
      cgttaacta caaatgaca ttattgatc tgagatgga 
      aagacactt gggacttca tagttcata aaaattagc 
      aattgttcc atgacagca aatttgata ctcatattg 
      atattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY