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Gene information
General information
TC014166 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7094945..7096792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014166
    iBeetle screen iB_09077

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014166 RA
      MASRVLLKK PFDLGKLVL RNQSTMSAF EIQHKTPLI 
      KKVEAIPVA MYGGRHHVT MLPGGGIGP ELMGYVKEV 
      FKYAGAPVD FEVVDIGDE NSDLDYALT SIKRNGVAI 
      KGNIETKSE SASVVSRNV AIRNELDLY VNILHCKSY 
      PGVPARQKD IDIVIIRQN TEGEYAMLE HESVDGVVE 
      SMKVVTESN SDRVARFAF EYAKKNGRK RVTTIHKAN 
      IMKLSDGLF LETSRRVAK DYPEIEHND MIIDNCCMQ 
      LVSKPHQFD VMIMTNLYG SIVSNVVCG LIGGAGLLS 
      GKNYGDHYA IFEPGTRNT GTAIAGKNI ANPIAMLSA 
      SVDMLNHLG HVQHAELIQ RAIEKTICE DKIHTPDLG 
      GSARSTDVV QRIIDHILQ ADIRPW
    CDS sequence
    • >TC014166 RA
      atggcatcg agagtatta ctgaaaaag cccttcgac 
      ctaggaaag ctcgtttta agaaaccaa tcaacaatg 
      tcagcgttt gaaattcag cacaaaacc ccccttatt 
      aaaaaagtg gaagctatc ccagttgct atgtacggg 
      ggccgccac cacgtcacg atgttgcca gggggcgga 
      attggcccc gaattgatg ggatacgtt aaagaggtt 
      ttcaagtat gcaggagcc cctgttgat tttgaagtc 
      gtggatatc ggggatgaa aacagtgat cttgactat 
      gccttaact tcaatcaag cgaaacggc gttgccatc 
      aaaggcaat attgaaacc aaaagcgag agcgcgagt 
      gtcgtttcc agaaatgtg gccatcaga aacgaacta 
      gatttatac gtaaatatt ttacactgt aagtcatat 
      ccgggagtt cctgcgaga caaaaagat atcgacatt 
      gtcatcatc agacaaaat actgaggga gagtatgcc 
      atgctggag cacgagagc gttgatggc gttgttgaa 
      agtatgaaa gtcgtgacg gagtcaaac tcagataga 
      gtcgcgcgg ttcgccttt gaatatgcc aaaaaaaac 
      gggcgtaaa agagttaca acaatacac aaagccaac 
      attatgaaa ctttcagat ggtcttttc ttagaaacg 
      tcaagacgg gtcgctaag gattaccca gaaattgag 
      cacaatgac atgattatc gataattgt tgcatgcaa 
      ctggtatca aagccacac cagttcgac gtaatgatc 
      atgactaat ttgtacgga agtatagtc tctaacgta 
      gtttgtgga ttgatagga ggcgctggt ctcttatct 
      ggtaaaaat tacggtgat cattatgca attttcgag 
      cctggcact agaaataca gggacggca attgccgga 
      aaaaatatc gccaaccct atcgccatg ctgagtgcc 
      tcagttgat atgttaaac catctggga catgtacaa 
      cacgctgaa cttatccag cgcgccatc gagaaaacc 
      atctgcgag gataaaatc cacactcct gatttgggt 
      ggttccgct aggagtacg gacgtcgtc cagagaatt 
      atcgaccac atcttacaa gccgatatt agaccttgg 
      taa
    mRNA sequence
    • >TC014166 RA
      atcgaagtt tatattcat ctcagtttc tatttttga 
      ataaatttt gtagtatta ccgcagtct ttaagccac 
      ataaacacc aaaatggca tcgagagta ttactgaaa 
      aagcccttc gacctagga aagctcgtt ttaagaaac 
      caatcaaca atgtcagcg tttgaaatt cagcacaaa 
      acccccctt attaaaaaa gtggaagct atcccagtt 
      gctatgtac gggggccgc caccacgtc acgatgttg 
      ccagggggc ggaattggc cccgaattg atgggatac 
      gttaaagag gttttcaag tatgcagga gcccctgtt 
      gattttgaa gtcgtggat atcggggat gaaaacagt 
      gatcttgac tatgcctta acttcaatc aagcgaaac 
      ggcgttgcc atcaaaggc aatattgaa accaaaagc 
      gagagcgcg agtgtcgtt tccagaaat gtggccatc 
      agaaacgaa ctagattta tacgtaaat attttacac 
      tgtaagtca tatccggga gttcctgcg agacaaaaa 
      gatatcgac attgtcatc atcagacaa aatactgag 
      ggagagtat gccatgctg gagcacgag agcgttgat 
      ggcgttgtt gaaagtatg aaagtcgtg acggagtca 
      aactcagat agagtcgcg cggttcgcc tttgaatat 
      gccaaaaaa aacgggcgt aaaagagtt acaacaata 
      cacaaagcc aacattatg aaactttca gatggtctt 
      ttcttagaa acgtcaaga cgggtcgct aaggattac 
      ccagaaatt gagcacaat gacatgatt atcgataat 
      tgttgcatg caactggta tcaaagcca caccagttc 
      gacgtaatg atcatgact aatttgtac ggaagtata 
      gtctctaac gtagtttgt ggattgata ggaggcgct 
      ggtctctta tctggtaaa aattacggt gatcattat 
      gcaattttc gagcctggc actagaaat acagggacg 
      gcaattgcc ggaaaaaat atcgccaac cctatcgcc 
      atgctgagt gcctcagtt gatatgtta aaccatctg 
      ggacatgta caacacgct gaacttatc cagcgcgcc 
      atcgagaaa accatctgc gaggataaa atccacact 
      cctgatttg ggtggttcc gctaggagt acggacgtc 
      gtccagaga attatcgac cacatctta caagccgat 
      attagacct tggtaatta tttttttta ttttggttg 
      tggtgcttg ctaacattt atgttgact aataataag 
      ttgtgcaat gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY