General information | |||
TC014137 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 6478870..6485324 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC014137 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09076 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
mature egg not present - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014137 RA
MEALIPVIN KLQDVFNTV GSDAIQLPQ IVVLGSQSS GKSSVIESL VGKSFLPRG TGIVTRVPL ILQLVYCPK DDREHRAAI DGTMDVEEW GVFLHCKSK IFKDFEEIR QEIENETNK IAGSNKGIC AEPINLKIY STKVVNLTL VDLPGITKV PVGDQPPDI EQQIRELVL KYIANPNSI ILAVVTANT DMATSESLA IAKDVDPDG RRTLAVVTK LDLMDAGTD AIDILCGRV IPVKLGIIG VVNRSQQDI MNNKSIKEA LKDEATFLQ SKYPTLATR NGTPYLAKT LNRLLMHHI RDCLPDLKT RVNLMMSQF QSLLNSYGE DISDKSKTL LQIITKFAS AYCSTIEGT ARNIETTEL CGGARICYI FHETFGRTL DSIHPLAGL TKMDILTAI RNANGPRPA LFVPEVSFE LLVKRQIRR LEEPSLRCV ELIHEEMQR IIQHCGSEV QQEMLRFPK LYEKIVDVV TQLLRRRLP TTNQMVENL VQIELAYIN TKHPDFYKE IAMVPSMIK SGDGSRPNR PKSMLIKHH NTVAETSEL VRSSSNPHL NEDATTWVS NLTSSQNGT DTALTNHIS GVPESVKNE EIKSVLSPV KPVNLLPQV PINSSSFRK LSENEEHDC EIIERLIKS YFYIVRKSI QDTVPKAVM HFLVNFVKD NLQSELVTH LYKADNADQ LLDESEHIA QRRKEAADM LKALQKAAH IISEIRETH MW
- >TC014137 RA
atggaagct ttaattcct gttatcaat aaattgcaa gacgttttt aacaccgtc ggatcagat gccattcaa ttaccccaa attgtggtc ttgggatca caaagttcg ggcaagagt tcagtgatt gaaagctta gttggaaag tcgttttta ccaagagga acagggatt gttacaaga gtacccctt attttacaa ttagtttat tgtcctaag gatgatagg gagcacaga gctgcaatc gatgggaca atggatgtg gaagagtgg ggcgttttt ctacattgc aaaagcaaa attttcaaa gactttgaa gaaatccgt caggaaatc gagaacgag accaacaaa attgctgga agtaacaaa ggcatttgt gccgaaccc atcaatttg aaaatttac tctacaaag gttgtgaat ttaacttta gtggattta ccgggcatt accaaggta ccagtcgga gaccagcct ccagacatt gaacaacaa attcgcgaa ttagtccta aaatacatc gctaatcca aattcaata atattagct gtagtaacg gcaaacaca gacatggcg acttccgaa agcttagca atcgcgaaa gatgttgat cctgatggt cgcagaaca ttggctgtc gttacgaaa cttgactta atggacgca ggtactgac gccattgat attctttgc ggccgagtt attccagta aaattaggc ataatcggt gtcgtaaat cgctcacaa caagatatt atgaacaac aaatcgata aaagaggcc ttaaaagac gaagcgaca tttttacaa agcaaatat cccacttta gccactcgc aacggtact ccctacctt gccaaaacc ttaaatagg cttttaatg caccacatt agagactgt ttacctgac ttgaagact cgcgtgaac ttaatgatg tcccagttc caatcactg cttaactca tacggcgaa gatatttcc gataagagc aaaactttg ctccagatt atcacaaaa ttcgcctca gcttactgc tccacaatt gaaggcact gcccgaaac attgaaacg actgaattg tgtggaggg gcgagaatt tgctacatt tttcacgag actttcggg aggacttta gactcgatc caccccttg gccggtttg acaaaaatg gatatttta accgccatt cggaatgca aatgggcct cgacctgcc cttttcgtc ccggaagtt tccttcgaa ttactagtc aagaggcaa atccgacgt cttgaagaa ccatcactg cgttgcgtt gaattaatc cacgaagaa atgcaacgt attatacaa cattgtgga agcgaagtg cagcaagaa atgctgcgg tttcccaaa ctgtacgaa aaaatcgta gatgttgta acacagtta ttgcggaga cgtcttccg acgaccaac caaatggtg gaaaattta gtgcaaatc gaattggcg tatattaat accaaacat cccgatttt tataaagaa atagcaatg gtgccatcg atgattaaa tccggggac ggatctcgt ccaaatcga ccaaagagt atgctaatc aaacaccat aatacagtg gcggagact tccgagttg gtgcgttct tcgagcaac cctcacttg aatgaagat gcgacaact tgggtgtct aatttaacc tcctcccaa aacggaacg gataccgct ctgacgaat cacatcagc ggtgtgccc gaaagcgta aaaaatgag gagataaaa tcagttctt agtcctgtg aaacctgtc aatttgtta ccacaagtt ccaatcaac agcagttcg ttcagaaag ctttctgag aatgaggaa cacgactgt gaaattatt gaacgcttg attaaatcg tatttctac atcgttcgt aaatcaatt caggataca gtcccgaag gcggtaatg catttctta gtgaatttt gttaaagac aatttgcaa tccgagtta gtgacacat ttgtataaa gctgacaat gctgatcag ttgttggac gagtctgag cacattgct cagagacgc aaagaagcc gcagatatg ttaaaagca cttcaaaaa gctgcacac atcattagc gaaattaga gaaacacac atgtggtaa
- >TC014137 RA
caaaactaa actgacaca aaaaatctg attgtccgc ttttcagtt gtggattta acgttcaga gcaattcgt ttattaaaa ttaacgccc agttttata ttgagtgtt gtgtgattt taaacgatg ctggtatag ctaccagtc tgtttaaga ttgatagtt taaaaacta gctcgcagt aatcgatat ttttggaaa tcgaccggt tataaagac aatggaagc tttaattcc tgttatcaa taaattgca agacgtttt taacaccgt cggatcaga tgccattca attacccca aattgtggt cttgggatc acaaagttc gggcaagag ttcagtgat tgaaagctt agttggaaa gtcgttttt accaagagg aacagggat tgttacaag agtacccct tattttaca attagttta ttgtcctaa ggatgatag ggagcacag agctgcaat cgatgggac aatggatgt ggaagagtg gggcgtttt tctacattg caaaagcaa aattttcaa agactttga agaaatccg tcaggaaat cgagaacga gaccaacaa aattgctgg aagtaacaa aggcatttg tgccgaacc catcaattt gaaaattta ctctacaaa ggttgtgaa tttaacttt agtggattt accgggcat taccaaggt accagtcgg agaccagcc tccagacat tgaacaaca aattcgcga attagtcct aaaatacat cgctaatcc aaattcaat aatattagc tgtagtaac ggcaaacac agacatggc gacttccga aagcttagc aatcgcgaa agatgttga tcctgatgg tcgcagaac attggctgt cgttacgaa acttgactt aatggacgc aggtactga cgccattga tattctttg cggccgagt tattccagt aaaattagg cataatcgg tgtcgtaaa tcgctcaca acaagatat tatgaacaa caaatcgat aaaagaggc cttaaaaga cgaagcgac atttttaca aagcaaata tcccacttt agccactcg caacggtac tccctacct tgccaaaac cttaaatag gcttttaat gcaccacat tagagactg tttacctga cttgaagac tcgcgtgaa cttaatgat gtcccagtt ccaatcact gcttaactc atacggcga agatatttc cgataagag caaaacttt gctccagat tatcacaaa attcgcctc agcttactg ctccacaat tgaaggcac tgcccgaaa cattgaaac gactgaatt gtgtggagg ggcgagaat ttgctacat ttttcacga gactttcgg gaggacttt agactcgat ccacccctt ggccggttt gacaaaaat ggatatttt aaccgccat tcggaatgc aaatgggcc tcgacctgc ccttttcgt cccggaagt ttccttcga attactagt caagaggca aatccgacg tcttgaaga accatcact gcgttgcgt tgaattaat ccacgaaga aatgcaacg tattataca acattgtgg aagcgaagt gcagcaaga aatgctgcg gtttcccaa actgtacga aaaaatcgt agatgttgt aacacagtt attgcggag acgtcttcc gacgaccaa ccaaatggt ggaaaattt agtgcaaat cgaattggc gtatattaa taccaaaca tcccgattt ttataaaga aatagcaat ggtgccatc gatgattaa atccgggga cggatctcg tccaaatcg accaaagag tatgctaat caaacacca taatacagt ggcggagac ttccgagtt ggtgcgttc ttcgagcaa ccctcactt gaatgaaga tgcgacaac ttgggtgtc taatttaac ctcctccca aaacggaac ggataccgc tctgacgaa tcacatcag cggtgtgcc cgaaagcgt aaaaaatga ggagataaa atcagttct tagtcctgt gaaacctgt caatttgtt accacaagt tccaatcaa cagcagttc gttcagaaa gctttctga gaatgagga acacgactg tgaaattat tgaacgctt gattaaatc gtatttcta catcgttcg taaatcaat tcaggatac agtcccgaa ggcggtaat gcatttctt agtgaattt tgttaaaga caatttgca atccgagtt agtgacaca tttgtataa agctgacaa tgctgatca gttgttgga cgagtctga gcacattgc tcagagacg caaagaagc cgcagatat gttaaaagc acttcaaaa agctgcaca catcattag cgaaattag agaaacaca catgtggta aacttgcag ccatattct ccctagtca agagtaagc gtgtactgg ccctcgatg ttaaagctg tatttaatc tttacttaa aaggataag agtaacact gaacgattt cggaatgtc ttgttcaaa aacttcata aagcgaaaa cggatattt gttgaagtt ttataaaaa tttaattac cttcctgta aataattgt acaattttt atcaattct gacatcttt gtaaagttc atcgattct cattttggt ctgatatta gacttgtta ctctctttt tagttggtg cattactgt agaaatcac tttttgatg ttacacgcg ccatagttg tatttcctc gtatttcaa tatttttga tgcattttt gttctagcc tagttgcgc atcaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
