Status
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Gene information
General information
TC014137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6478870..6485324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014137
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09076

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014137 RA
      MEALIPVIN KLQDVFNTV GSDAIQLPQ IVVLGSQSS 
      GKSSVIESL VGKSFLPRG TGIVTRVPL ILQLVYCPK 
      DDREHRAAI DGTMDVEEW GVFLHCKSK IFKDFEEIR 
      QEIENETNK IAGSNKGIC AEPINLKIY STKVVNLTL 
      VDLPGITKV PVGDQPPDI EQQIRELVL KYIANPNSI 
      ILAVVTANT DMATSESLA IAKDVDPDG RRTLAVVTK 
      LDLMDAGTD AIDILCGRV IPVKLGIIG VVNRSQQDI 
      MNNKSIKEA LKDEATFLQ SKYPTLATR NGTPYLAKT 
      LNRLLMHHI RDCLPDLKT RVNLMMSQF QSLLNSYGE 
      DISDKSKTL LQIITKFAS AYCSTIEGT ARNIETTEL 
      CGGARICYI FHETFGRTL DSIHPLAGL TKMDILTAI 
      RNANGPRPA LFVPEVSFE LLVKRQIRR LEEPSLRCV 
      ELIHEEMQR IIQHCGSEV QQEMLRFPK LYEKIVDVV 
      TQLLRRRLP TTNQMVENL VQIELAYIN TKHPDFYKE 
      IAMVPSMIK SGDGSRPNR PKSMLIKHH NTVAETSEL 
      VRSSSNPHL NEDATTWVS NLTSSQNGT DTALTNHIS 
      GVPESVKNE EIKSVLSPV KPVNLLPQV PINSSSFRK 
      LSENEEHDC EIIERLIKS YFYIVRKSI QDTVPKAVM 
      HFLVNFVKD NLQSELVTH LYKADNADQ LLDESEHIA 
      QRRKEAADM LKALQKAAH IISEIRETH MW
    CDS sequence
    • >TC014137 RA
      atggaagct ttaattcct gttatcaat aaattgcaa 
      gacgttttt aacaccgtc ggatcagat gccattcaa 
      ttaccccaa attgtggtc ttgggatca caaagttcg 
      ggcaagagt tcagtgatt gaaagctta gttggaaag 
      tcgttttta ccaagagga acagggatt gttacaaga 
      gtacccctt attttacaa ttagtttat tgtcctaag 
      gatgatagg gagcacaga gctgcaatc gatgggaca 
      atggatgtg gaagagtgg ggcgttttt ctacattgc 
      aaaagcaaa attttcaaa gactttgaa gaaatccgt 
      caggaaatc gagaacgag accaacaaa attgctgga 
      agtaacaaa ggcatttgt gccgaaccc atcaatttg 
      aaaatttac tctacaaag gttgtgaat ttaacttta 
      gtggattta ccgggcatt accaaggta ccagtcgga 
      gaccagcct ccagacatt gaacaacaa attcgcgaa 
      ttagtccta aaatacatc gctaatcca aattcaata 
      atattagct gtagtaacg gcaaacaca gacatggcg 
      acttccgaa agcttagca atcgcgaaa gatgttgat 
      cctgatggt cgcagaaca ttggctgtc gttacgaaa 
      cttgactta atggacgca ggtactgac gccattgat 
      attctttgc ggccgagtt attccagta aaattaggc 
      ataatcggt gtcgtaaat cgctcacaa caagatatt 
      atgaacaac aaatcgata aaagaggcc ttaaaagac 
      gaagcgaca tttttacaa agcaaatat cccacttta 
      gccactcgc aacggtact ccctacctt gccaaaacc 
      ttaaatagg cttttaatg caccacatt agagactgt 
      ttacctgac ttgaagact cgcgtgaac ttaatgatg 
      tcccagttc caatcactg cttaactca tacggcgaa 
      gatatttcc gataagagc aaaactttg ctccagatt 
      atcacaaaa ttcgcctca gcttactgc tccacaatt 
      gaaggcact gcccgaaac attgaaacg actgaattg 
      tgtggaggg gcgagaatt tgctacatt tttcacgag 
      actttcggg aggacttta gactcgatc caccccttg 
      gccggtttg acaaaaatg gatatttta accgccatt 
      cggaatgca aatgggcct cgacctgcc cttttcgtc 
      ccggaagtt tccttcgaa ttactagtc aagaggcaa 
      atccgacgt cttgaagaa ccatcactg cgttgcgtt 
      gaattaatc cacgaagaa atgcaacgt attatacaa 
      cattgtgga agcgaagtg cagcaagaa atgctgcgg 
      tttcccaaa ctgtacgaa aaaatcgta gatgttgta 
      acacagtta ttgcggaga cgtcttccg acgaccaac 
      caaatggtg gaaaattta gtgcaaatc gaattggcg 
      tatattaat accaaacat cccgatttt tataaagaa 
      atagcaatg gtgccatcg atgattaaa tccggggac 
      ggatctcgt ccaaatcga ccaaagagt atgctaatc 
      aaacaccat aatacagtg gcggagact tccgagttg 
      gtgcgttct tcgagcaac cctcacttg aatgaagat 
      gcgacaact tgggtgtct aatttaacc tcctcccaa 
      aacggaacg gataccgct ctgacgaat cacatcagc 
      ggtgtgccc gaaagcgta aaaaatgag gagataaaa 
      tcagttctt agtcctgtg aaacctgtc aatttgtta 
      ccacaagtt ccaatcaac agcagttcg ttcagaaag 
      ctttctgag aatgaggaa cacgactgt gaaattatt 
      gaacgcttg attaaatcg tatttctac atcgttcgt 
      aaatcaatt caggataca gtcccgaag gcggtaatg 
      catttctta gtgaatttt gttaaagac aatttgcaa 
      tccgagtta gtgacacat ttgtataaa gctgacaat 
      gctgatcag ttgttggac gagtctgag cacattgct 
      cagagacgc aaagaagcc gcagatatg ttaaaagca 
      cttcaaaaa gctgcacac atcattagc gaaattaga 
      gaaacacac atgtggtaa
    mRNA sequence
    • >TC014137 RA
      caaaactaa actgacaca aaaaatctg attgtccgc 
      ttttcagtt gtggattta acgttcaga gcaattcgt 
      ttattaaaa ttaacgccc agttttata ttgagtgtt 
      gtgtgattt taaacgatg ctggtatag ctaccagtc 
      tgtttaaga ttgatagtt taaaaacta gctcgcagt 
      aatcgatat ttttggaaa tcgaccggt tataaagac 
      aatggaagc tttaattcc tgttatcaa taaattgca 
      agacgtttt taacaccgt cggatcaga tgccattca 
      attacccca aattgtggt cttgggatc acaaagttc 
      gggcaagag ttcagtgat tgaaagctt agttggaaa 
      gtcgttttt accaagagg aacagggat tgttacaag 
      agtacccct tattttaca attagttta ttgtcctaa 
      ggatgatag ggagcacag agctgcaat cgatgggac 
      aatggatgt ggaagagtg gggcgtttt tctacattg 
      caaaagcaa aattttcaa agactttga agaaatccg 
      tcaggaaat cgagaacga gaccaacaa aattgctgg 
      aagtaacaa aggcatttg tgccgaacc catcaattt 
      gaaaattta ctctacaaa ggttgtgaa tttaacttt 
      agtggattt accgggcat taccaaggt accagtcgg 
      agaccagcc tccagacat tgaacaaca aattcgcga 
      attagtcct aaaatacat cgctaatcc aaattcaat 
      aatattagc tgtagtaac ggcaaacac agacatggc 
      gacttccga aagcttagc aatcgcgaa agatgttga 
      tcctgatgg tcgcagaac attggctgt cgttacgaa 
      acttgactt aatggacgc aggtactga cgccattga 
      tattctttg cggccgagt tattccagt aaaattagg 
      cataatcgg tgtcgtaaa tcgctcaca acaagatat 
      tatgaacaa caaatcgat aaaagaggc cttaaaaga 
      cgaagcgac atttttaca aagcaaata tcccacttt 
      agccactcg caacggtac tccctacct tgccaaaac 
      cttaaatag gcttttaat gcaccacat tagagactg 
      tttacctga cttgaagac tcgcgtgaa cttaatgat 
      gtcccagtt ccaatcact gcttaactc atacggcga 
      agatatttc cgataagag caaaacttt gctccagat 
      tatcacaaa attcgcctc agcttactg ctccacaat 
      tgaaggcac tgcccgaaa cattgaaac gactgaatt 
      gtgtggagg ggcgagaat ttgctacat ttttcacga 
      gactttcgg gaggacttt agactcgat ccacccctt 
      ggccggttt gacaaaaat ggatatttt aaccgccat 
      tcggaatgc aaatgggcc tcgacctgc ccttttcgt 
      cccggaagt ttccttcga attactagt caagaggca 
      aatccgacg tcttgaaga accatcact gcgttgcgt 
      tgaattaat ccacgaaga aatgcaacg tattataca 
      acattgtgg aagcgaagt gcagcaaga aatgctgcg 
      gtttcccaa actgtacga aaaaatcgt agatgttgt 
      aacacagtt attgcggag acgtcttcc gacgaccaa 
      ccaaatggt ggaaaattt agtgcaaat cgaattggc 
      gtatattaa taccaaaca tcccgattt ttataaaga 
      aatagcaat ggtgccatc gatgattaa atccgggga 
      cggatctcg tccaaatcg accaaagag tatgctaat 
      caaacacca taatacagt ggcggagac ttccgagtt 
      ggtgcgttc ttcgagcaa ccctcactt gaatgaaga 
      tgcgacaac ttgggtgtc taatttaac ctcctccca 
      aaacggaac ggataccgc tctgacgaa tcacatcag 
      cggtgtgcc cgaaagcgt aaaaaatga ggagataaa 
      atcagttct tagtcctgt gaaacctgt caatttgtt 
      accacaagt tccaatcaa cagcagttc gttcagaaa 
      gctttctga gaatgagga acacgactg tgaaattat 
      tgaacgctt gattaaatc gtatttcta catcgttcg 
      taaatcaat tcaggatac agtcccgaa ggcggtaat 
      gcatttctt agtgaattt tgttaaaga caatttgca 
      atccgagtt agtgacaca tttgtataa agctgacaa 
      tgctgatca gttgttgga cgagtctga gcacattgc 
      tcagagacg caaagaagc cgcagatat gttaaaagc 
      acttcaaaa agctgcaca catcattag cgaaattag 
      agaaacaca catgtggta aacttgcag ccatattct 
      ccctagtca agagtaagc gtgtactgg ccctcgatg 
      ttaaagctg tatttaatc tttacttaa aaggataag 
      agtaacact gaacgattt cggaatgtc ttgttcaaa 
      aacttcata aagcgaaaa cggatattt gttgaagtt 
      ttataaaaa tttaattac cttcctgta aataattgt 
      acaattttt atcaattct gacatcttt gtaaagttc 
      atcgattct cattttggt ctgatatta gacttgtta 
      ctctctttt tagttggtg cattactgt agaaatcac 
      tttttgatg ttacacgcg ccatagttg tatttcctc 
      gtatttcaa tatttttga tgcattttt gttctagcc 
      tagttgcgc atcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY