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Gene information
General information
TC014137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 6478870..6485324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014137
    iBeetle screen iB_09076

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014137 RA
      MEALIPVIN KLQDVFNTV GSDAIQLPQ IVVLGSQSS 
GKSSVIESL VGKSFLPRG TGIVTRVPL ILQLVYCPK 
DDREHRAAI DGTMDVEEW GVFLHCKSK IFKDFEEIR 
QEIENETNK IAGSNKGIC AEPINLKIY STKVVNLTL 
VDLPGITKV PVGDQPPDI EQQIRELVL KYIANPNSI 
ILAVVTANT DMATSESLA IAKDVDPDG RRTLAVVTK 
LDLMDAGTD AIDILCGRV IPVKLGIIG VVNRSQQDI 
MNNKSIKEA LKDEATFLQ SKYPTLATR NGTPYLAKT 
LNRLLMHHI RDCLPDLKT RVNLMMSQF QSLLNSYGE 
DISDKSKTL LQIITKFAS AYCSTIEGT ARNIETTEL 
CGGARICYI FHETFGRTL DSIHPLAGL TKMDILTAI 
RNANGPRPA LFVPEVSFE LLVKRQIRR LEEPSLRCV 
ELIHEEMQR IIQHCGSEV QQEMLRFPK LYEKIVDVV 
TQLLRRRLP TTNQMVENL VQIELAYIN TKHPDFYKE 
IAMVPSMIK SGDGSRPNR PKSMLIKHH NTVAETSEL 
VRSSSNPHL NEDATTWVS NLTSSQNGT DTALTNHIS 
GVPESVKNE EIKSVLSPV KPVNLLPQV PINSSSFRK 
LSENEEHDC EIIERLIKS YFYIVRKSI QDTVPKAVM 
HFLVNFVKD NLQSELVTH LYKADNADQ LLDESEHIA 
QRRKEAADM LKALQKAAH IISEIRETH MW
    CDS sequence
    • >TC014137 RA
      atggaagct ttaattcct gttatcaat aaattgcaa 
gacgttttt aacaccgtc ggatcagat gccattcaa 
ttaccccaa attgtggtc ttgggatca caaagttcg 
ggcaagagt tcagtgatt gaaagctta gttggaaag 
tcgttttta ccaagagga acagggatt gttacaaga 
gtacccctt attttacaa ttagtttat tgtcctaag 
gatgatagg gagcacaga gctgcaatc gatgggaca 
atggatgtg gaagagtgg ggcgttttt ctacattgc 
aaaagcaaa attttcaaa gactttgaa gaaatccgt 
caggaaatc gagaacgag accaacaaa attgctgga 
agtaacaaa ggcatttgt gccgaaccc atcaatttg 
aaaatttac tctacaaag gttgtgaat ttaacttta 
gtggattta ccgggcatt accaaggta ccagtcgga 
gaccagcct ccagacatt gaacaacaa attcgcgaa 
ttagtccta aaatacatc gctaatcca aattcaata 
atattagct gtagtaacg gcaaacaca gacatggcg 
acttccgaa agcttagca atcgcgaaa gatgttgat 
cctgatggt cgcagaaca ttggctgtc gttacgaaa 
cttgactta atggacgca ggtactgac gccattgat 
attctttgc ggccgagtt attccagta aaattaggc 
ataatcggt gtcgtaaat cgctcacaa caagatatt 
atgaacaac aaatcgata aaagaggcc ttaaaagac 
gaagcgaca tttttacaa agcaaatat cccacttta 
gccactcgc aacggtact ccctacctt gccaaaacc 
ttaaatagg cttttaatg caccacatt agagactgt 
ttacctgac ttgaagact cgcgtgaac ttaatgatg 
tcccagttc caatcactg cttaactca tacggcgaa 
gatatttcc gataagagc aaaactttg ctccagatt 
atcacaaaa ttcgcctca gcttactgc tccacaatt 
gaaggcact gcccgaaac attgaaacg actgaattg 
tgtggaggg gcgagaatt tgctacatt tttcacgag 
actttcggg aggacttta gactcgatc caccccttg 
gccggtttg acaaaaatg gatatttta accgccatt 
cggaatgca aatgggcct cgacctgcc cttttcgtc 
ccggaagtt tccttcgaa ttactagtc aagaggcaa 
atccgacgt cttgaagaa ccatcactg cgttgcgtt 
gaattaatc cacgaagaa atgcaacgt attatacaa 
cattgtgga agcgaagtg cagcaagaa atgctgcgg 
tttcccaaa ctgtacgaa aaaatcgta gatgttgta 
acacagtta ttgcggaga cgtcttccg acgaccaac 
caaatggtg gaaaattta gtgcaaatc gaattggcg 
tatattaat accaaacat cccgatttt tataaagaa 
atagcaatg gtgccatcg atgattaaa tccggggac 
ggatctcgt ccaaatcga ccaaagagt atgctaatc 
aaacaccat aatacagtg gcggagact tccgagttg 
gtgcgttct tcgagcaac cctcacttg aatgaagat 
gcgacaact tgggtgtct aatttaacc tcctcccaa 
aacggaacg gataccgct ctgacgaat cacatcagc 
ggtgtgccc gaaagcgta aaaaatgag gagataaaa 
tcagttctt agtcctgtg aaacctgtc aatttgtta 
ccacaagtt ccaatcaac agcagttcg ttcagaaag 
ctttctgag aatgaggaa cacgactgt gaaattatt 
gaacgcttg attaaatcg tatttctac atcgttcgt 
aaatcaatt caggataca gtcccgaag gcggtaatg 
catttctta gtgaatttt gttaaagac aatttgcaa 
tccgagtta gtgacacat ttgtataaa gctgacaat 
gctgatcag ttgttggac gagtctgag cacattgct 
cagagacgc aaagaagcc gcagatatg ttaaaagca 
cttcaaaaa gctgcacac atcattagc gaaattaga 
gaaacacac atgtggtaa
    mRNA sequence
    • >TC014137 RA
      caaaactaa actgacaca aaaaatctg attgtccgc 
ttttcagtt gtggattta acgttcaga gcaattcgt 
ttattaaaa ttaacgccc agttttata ttgagtgtt 
gtgtgattt taaacgatg ctggtatag ctaccagtc 
tgtttaaga ttgatagtt taaaaacta gctcgcagt 
aatcgatat ttttggaaa tcgaccggt tataaagac 
aatggaagc tttaattcc tgttatcaa taaattgca 
agacgtttt taacaccgt cggatcaga tgccattca 
attacccca aattgtggt cttgggatc acaaagttc 
gggcaagag ttcagtgat tgaaagctt agttggaaa 
gtcgttttt accaagagg aacagggat tgttacaag 
agtacccct tattttaca attagttta ttgtcctaa 
ggatgatag ggagcacag agctgcaat cgatgggac 
aatggatgt ggaagagtg gggcgtttt tctacattg 
caaaagcaa aattttcaa agactttga agaaatccg 
tcaggaaat cgagaacga gaccaacaa aattgctgg 
aagtaacaa aggcatttg tgccgaacc catcaattt 
gaaaattta ctctacaaa ggttgtgaa tttaacttt 
agtggattt accgggcat taccaaggt accagtcgg 
agaccagcc tccagacat tgaacaaca aattcgcga 
attagtcct aaaatacat cgctaatcc aaattcaat 
aatattagc tgtagtaac ggcaaacac agacatggc 
gacttccga aagcttagc aatcgcgaa agatgttga 
tcctgatgg tcgcagaac attggctgt cgttacgaa 
acttgactt aatggacgc aggtactga cgccattga 
tattctttg cggccgagt tattccagt aaaattagg 
cataatcgg tgtcgtaaa tcgctcaca acaagatat 
tatgaacaa caaatcgat aaaagaggc cttaaaaga 
cgaagcgac atttttaca aagcaaata tcccacttt 
agccactcg caacggtac tccctacct tgccaaaac 
cttaaatag gcttttaat gcaccacat tagagactg 
tttacctga cttgaagac tcgcgtgaa cttaatgat 
gtcccagtt ccaatcact gcttaactc atacggcga 
agatatttc cgataagag caaaacttt gctccagat 
tatcacaaa attcgcctc agcttactg ctccacaat 
tgaaggcac tgcccgaaa cattgaaac gactgaatt 
gtgtggagg ggcgagaat ttgctacat ttttcacga 
gactttcgg gaggacttt agactcgat ccacccctt 
ggccggttt gacaaaaat ggatatttt aaccgccat 
tcggaatgc aaatgggcc tcgacctgc ccttttcgt 
cccggaagt ttccttcga attactagt caagaggca 
aatccgacg tcttgaaga accatcact gcgttgcgt 
tgaattaat ccacgaaga aatgcaacg tattataca 
acattgtgg aagcgaagt gcagcaaga aatgctgcg 
gtttcccaa actgtacga aaaaatcgt agatgttgt 
aacacagtt attgcggag acgtcttcc gacgaccaa 
ccaaatggt ggaaaattt agtgcaaat cgaattggc 
gtatattaa taccaaaca tcccgattt ttataaaga 
aatagcaat ggtgccatc gatgattaa atccgggga 
cggatctcg tccaaatcg accaaagag tatgctaat 
caaacacca taatacagt ggcggagac ttccgagtt 
ggtgcgttc ttcgagcaa ccctcactt gaatgaaga 
tgcgacaac ttgggtgtc taatttaac ctcctccca 
aaacggaac ggataccgc tctgacgaa tcacatcag 
cggtgtgcc cgaaagcgt aaaaaatga ggagataaa 
atcagttct tagtcctgt gaaacctgt caatttgtt 
accacaagt tccaatcaa cagcagttc gttcagaaa 
gctttctga gaatgagga acacgactg tgaaattat 
tgaacgctt gattaaatc gtatttcta catcgttcg 
taaatcaat tcaggatac agtcccgaa ggcggtaat 
gcatttctt agtgaattt tgttaaaga caatttgca 
atccgagtt agtgacaca tttgtataa agctgacaa 
tgctgatca gttgttgga cgagtctga gcacattgc 
tcagagacg caaagaagc cgcagatat gttaaaagc 
acttcaaaa agctgcaca catcattag cgaaattag 
agaaacaca catgtggta aacttgcag ccatattct 
ccctagtca agagtaagc gtgtactgg ccctcgatg 
ttaaagctg tatttaatc tttacttaa aaggataag 
agtaacact gaacgattt cggaatgtc ttgttcaaa 
aacttcata aagcgaaaa cggatattt gttgaagtt 
ttataaaaa tttaattac cttcctgta aataattgt 
acaattttt atcaattct gacatcttt gtaaagttc 
atcgattct cattttggt ctgatatta gacttgtta 
ctctctttt tagttggtg cattactgt agaaatcac 
tttttgatg ttacacgcg ccatagttg tatttcctc 
gtatttcaa tatttttga tgcattttt gttctagcc 
tagttgcgc atcaa
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