Status
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Gene information
General information
TC033074
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013694,TC013693 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5905758..5920783
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033074
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09073

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033074 RA
      MQNVQEVMP SVRLNGVDK TVEVAASSG EVIKKFRVS 
QPREECRRH RLTTDRDQT SPLSPNQSD KHFQTSVVA 
DKYLLLEQA EGSSLYRCV DVNTQEELV CKIVSKDCH 
SLVSAHYRL DSHPRVSSL HEVVVGNRY LYLVYPKAH 
GDLHSYVRS RKRLRESEA KRLFKQIAE TVQVCHRNG 
IVLRDLKLR KFVFADGQR TELKLESLE DAVVLEEPE 
TDWLHDKRG CPAYVSPEI LKAGAHYSG KAADMWSLG 
VILYTMLVG RYPFSDSEH ASLFAKISR GHFVIPECL 
SSRARCLIR SLLRREPSE RITSEDILY HPWLAKEDR 
EWTSRACDQ LVPECSLYD D
    CDS sequence
    • >TC033074 RA
      atgcaaaac gtacaagaa gtgatgccg agtgttaga 
cttaacggg gtggacaag acggtggaa gttgcggcc 
tcctccggg gaagtgatt aaaaagttc cgggtgagc 
cagccgagg gaggagtgc cggaggcac aggctcacc 
accgaccgg gaccaaact tcgcccctg agccccaac 
caaagtgat aaacatttc cagacctcg gtggtcgcc 
gataagtat ttgctgctg gagcaggcc gagggcagc 
tccttgtac cggtgcgtg gacgtcaac acccaggag 
gagttggtc tgcaagatc gtttcaaag gactgtcat 
tcgttggta tcggcgcat tatcgcctc gattcgcat 
ccgagggtc tcatctttg cacgaagtc gtcgtaggt 
aatcgctac ttgtatttg gtttatccc aaggctcac 
ggcgaccta cattcgtac gtgaggtca aggaaaaga 
ctgagggaa tccgaagcc aagaggctc ttcaaacaa 
attgccgaa acagtacaa gtttgtcat aggaacgga 
atagtatta agggattta aagctgagg aaattcgtc 
ttcgctgat ggccaaagg acggagctg aaattagag 
tcactagaa gatgccgtc gtgctggaa gaaccggaa 
accgactgg ctacatgat aaaagagga tgtcctgct 
tacgtgagt ccggaaatt ctcaaagcc ggcgcccac 
tactcagga aaggccgca gatatgtgg tcgctagga 
gtcatcctt tacacgatg ctagtcgga agatacccc 
ttcagcgat tcggagcac gcgtccctg tttgcgaaa 
atctcccga ggccatttc gtaattcct gaatgtctg 
tcgtcccgt gcgagatgt ttaattcgc tcgctgttg 
cggcgagag ccttcggag cggatcacg tccgaagac 
atcttgtac cacccttgg ctggcgaag gaggacagg 
gagtggaca tcgcgggcg tgcgatcaa ttagtgccc 
gaatgtagt ctttacgac gactag
    mRNA sequence
    • >TC033074 RA
      cggagcagt agcgcgatg actttggtt gtgcgcaca 
ctcgccgtg gcagtgtta ttatagttt tgtgttaac 
accgttttg ttattattt agttgattc attttttgt 
ttcggttat tttgcagtt acgccttac atttcgtta 
cggctccga gaacgggaa ggaaggtgg agttgtgct 
aggataaca aattgactc aaaaaattt aatttattt 
tgtgttcgg tttgtgtgt gaagtgcga caatgcaaa 
acgtacaag aagtgatgc cgagtgtta gacttaacg 
gggtggaca agacggtgg aagttgcgg cctcctccg 
gggaagtga ttaaaaagt tccgggtga gccagccga 
gggaggagt gccggaggc acaggctca ccaccgacc 
gggaccaaa cttcgcccc tgagcccca accaaagtg 
ataaacatt tccagacct cggtggtcg ccgataagt 
atttgctgc tggagcagg ccgagggca gctccttgt 
accggtgcg tggacgtca acacccagg aggagttgg 
tctgcaaga tcgtttcaa aggactgtc attcgttgg 
tatcggcgc attatcgcc tcgattcgc atccgaggg 
tctcatctt tgcacgaag tcgtcgtag gtaatcgct 
acttgtatt tggtttatc ccaaggctc acggcgacc 
tacattcgt acgtgaggt caaggaaaa gactgaggg 
aatccgaag ccaagaggc tcttcaaac aaattgccg 
aaacagtac aagtttgtc ataggaacg gaatagtat 
taagggatt taaagctga ggaaattcg tcttcgctg 
atggccaaa ggacggagc tgaaattag agtcactag 
aagatgccg tcgtgctgg aagaaccgg aaaccgact 
ggctacatg ataaaagag gatgtcctg cttacgtga 
gtccggaaa ttctcaaag ccggcgccc actactcag 
gaaaggccg cagatatgt ggtcgctag gagtcatcc 
tttacacga tgctagtcg gaagatacc ccttcagcg 
attcggagc acgcgtccc tgtttgcga aaatctccc 
gaggccatt tcgtaattc ctgaatgtc tgtcgtccc 
gtgcgagat gtttaattc gctcgctgt tgcggcgag 
agccttcgg agcggatca cgtccgaag acatcttgt 
accaccctt ggctggcga aggaggaca gggagtgga 
catcgcggg cgtgcgatc aattagtgc ccgaatgta 
gtctttacg acgactagc tcgagggcg gaaaacgtg 
caagtttcg tgctgtgtt ttagtaata taaggaagc 
cattcgttt tttcttttg ttgttagga gtaatttat 
aattgtttt ttcctttta atcatttcg ttttaaacg 
ggtctgcgg ccggacttc gtaggtgat cgtcgttgt 
cttatttat ttgttttcg ccgatgttg ttatttacg 
gcacctatg gagcgcggt ctggtcatc caatgaaac 
acatttctg atattacga agtagttta aaatctcat 
catcaatgc gagtggttt atggactga attatttca 
agcatcacg atatcatag tgattaatt tgaagactg 
agcggttta ttattttgt gaccaattg tgcaattta 
gcttaattt tgctcaaaa attgtcaga tcaggagtg 
aagtttaat taatgagtg tttctcaag cgatatgac 
attttttct tgaatgttg gggagggag gtgtggagc 
aactggccc gattcttat gaaaggcga cttgtgcgt 
aaatagatg cactgcgtg ggagtgcaa tccaaagat 
ctttaagtt tttatttgt tttaaaaga attttcgtt 
gatttcgca agaggttgc gttagtttc ttcgtagga 
ttagttgta cataattgt taccccgtc tccactatt 
ttattacgc acaacggga gacatcggt ccagtcgct 
tcactaata acaaattgt caaaatcat gtgatcgtt 
aggtcaata ctaatgcaa tgtggaact taggctaag 
taagtgaaa taatttcat atcgtgatg ctactagga 
acgttatag tttgcaatg tgatctcgg tcaaattgt 
caaagtttg gccatcgac gtgtaggta tatttctgt 
ggaactgaa ttgtatata attttgttt ttatttcgt 
ttcctaatt tatcgtaag gtcctaggt taatctatg 
taaattttg tacatagct ttgcatttt atttgtgaa 
ttcgtttca tggttgttc tatgctgtt gtccataat 
aaaaaacat cattattt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY