Status
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Gene information
General information
TC011283 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 2086662..2090932
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011283
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011283 RA
      MSPIQDFHS GQIIFLTGA TGFLGKLVL EKLLRTCSE 
      LKKIYVLLR PKKGQSQHE RLEALLNQP CFEEMKVLT 
      PNFRQKIAI LHGDCGAPF LGLSLQDRT LLEEVTCVI 
      HSAAVVKFN VKLKNAIFT NVRAVRDLI ILARNMPKL 
      KSFVYVSTA FSHCVRHEI GEEFYDVGI NPEDVISMV 
      QGLDDVILE TLTPHILGK WPNCYVYSK ALAEQLFKN 
      ESLPFSIVR PAIVGSAYQ EPIPGWIDN FQGIVGISV 
      GVSMGVLRS LQIDPKLRA NVVPVDFVV NTVLASGWY 
      CQKSEKLIY NFAGSDLNP ITWDVVVNK LSQYAFKYP 
      MSNAIWYPN FSVTPSTAV HKIRVLFSH TLYAYFVDA 
      ILYLLGRKR IAVKKYKKI AELTDCLSY FTLGSWKFS 
      DENVRKLWM EMTANDRWN FNFDMEKLE WENYGENCV 
      AGGRIYLMK DPLETVPRA RKRLFALFV IHYGFIFLV 
      LYLLFKFIT WVI
    CDS sequence
    • >TC011283 RA
      atgtcaccc attcaagac ttccacagc ggccaaata 
      attttccta acaggtgcc acaggtttc ctcgggaaa 
      ctcgtgctg gaaaaactg ctccggact tgctcggag 
      ttgaaaaaa atatacgtc ctgttgagg ccaaagaaa 
      ggtcaaagt cagcatgaa cgactcgag gcgcttttg 
      aaccaacct tgttttgag gaaatgaaa gttcttact 
      cccaatttt cgccaaaaa attgcaatt ttgcatggg 
      gattgtggc gcccctttc ttggggctg agtctccag 
      gaccggact ttattggaa gaagtgacg tgtgttata 
      cattcagca gccgtggtt aagttcaac gttaagtta 
      aaaaatgcg atttttacg aacgtgagg gcagtgcga 
      gatttaata attttggcc agaaacatg ccgaaattg 
      aaaagtttt gtctacgtt tcaactgct ttttcgcat 
      tgtgttcga cacgaaatc ggggaggag ttttacgat 
      gttgggata aaccctgaa gacgttatt tcaatggtt 
      caagggcta gatgatgtt attttggaa acgctaact 
      ccacatatt ttgggtaaa tggcccaat tgttacgtc 
      tacagtaaa gccctggct gagcaactt ttcaaaaac 
      gagtctctt ccattttcc atagttcgc ccagcgatt 
      gttggttct gcataccaa gagccaatc cctggttgg 
      attgataat tttcaagga attgtggga atttcggtt 
      ggggtctca atgggcgtt ttgaggagt ctacagatt 
      gaccccaaa cttagagcc aatgtagtt cctgttgac 
      tttgtagtt aatacagta ctcgcttca gggtggtat 
      tgccaaaag tcagaaaaa ttaatttat aacttcgcg 
      ggaagtgat ttaaatcca attacttgg gacgtcgta 
      gtaaacaaa ctctcgcaa tacgccttc aagtaccca 
      atgagcaat gccatttgg tacccaaat ttttcagta 
      acccctagc acagctgtc cacaaaatt cgggtgctt 
      ttttcgcac accctgtat gcgtatttc gtcgacgcg 
      attttatac cttttggga agaaaacga attgcggtc 
      aaaaaatac aaaaaaatt gcggagctg actgattgt 
      ctctcatat ttcacgttg ggttcatgg aaattttct 
      gatgaaaat gtgagaaaa ctgtggatg gaaatgacg 
      gccaacgat cgttggaat tttaatttt gatatggaa 
      aaactcgag tgggaaaat tatggtgaa aattgtgtc 
      gccggaggc cgcatttac ttgatgaag gatcctctg 
      gaaacggtg ccaagagcg agaaagcgg ctttttgcc 
      ctgtttgtg attcactac ggatttata tttttagtt 
      ttgtatttg ttgttcaaa tttatcact tgggttatt 
      tga
    mRNA sequence
    • >TC011283 RA
      tacggccca acatgtcca aaattattc gccagtttt 
      cgtcacgat gtcacccat tcaagactt ccacagcgg 
      ccaaataat tttcctaac aggtgccac aggtttcct 
      cgggaaact cgtgctgga aaaactgct ccggacttg 
      ctcggagtt gaaaaaaat atacgtcct gttgaggcc 
      aaagaaagg tcaaagtca gcatgaacg actcgaggc 
      gcttttgaa ccaaccttg ttttgagga aatgaaagt 
      tcttactcc caattttcg ccaaaaaat tgcaatttt 
      gcatgggga ttgtggcgc ccctttctt ggggctgag 
      tctccagga ccggacttt attggaaga agtgacgtg 
      tgttataca ttcagcagc cgtggttaa gttcaacgt 
      taagttaaa aaatgcgat ttttacgaa cgtgagggc 
      agtgcgaga tttaataat tttggccag aaacatgcc 
      gaaattgaa aagttttgt ctacgtttc aactgcttt 
      ttcgcattg tgttcgaca cgaaatcgg ggaggagtt 
      ttacgatgt tgggataaa ccctgaaga cgttatttc 
      aatggttca agggctaga tgatgttat tttggaaac 
      gctaactcc acatatttt gggtaaatg gcccaattg 
      ttacgtcta cagtaaagc cctggctga gcaactttt 
      caaaaacga gtctcttcc attttccat agttcgccc 
      agcgattgt tggttctgc ataccaaga gccaatccc 
      tggttggat tgataattt tcaaggaat tgtgggaat 
      ttcggttgg ggtctcaat gggcgtttt gaggagtct 
      acagattga ccccaaact tagagccaa tgtagttcc 
      tgttgactt tgtagttaa tacagtact cgcttcagg 
      gtggtattg ccaaaagtc agaaaaatt aatttataa 
      cttcgcggg aagtgattt aaatccaat tacttggga 
      cgtcgtagt aaacaaact ctcgcaata cgccttcaa 
      gtacccaat gagcaatgc catttggta cccaaattt 
      ttcagtaac ccctagcac agctgtcca caaaattcg 
      ggtgctttt ttcgcacac cctgtatgc gtatttcgt 
      cgacgcgat tttatacct tttgggaag aaaacgaat 
      tgcggtcaa aaaatacaa aaaaattgc ggagctgac 
      tgattgtct ctcatattt cacgttggg ttcatggaa 
      attttctga tgaaaatgt gagaaaact gtggatgga 
      aatgacggc caacgatcg ttggaattt taattttga 
      tatggaaaa actcgagtg ggaaaatta tggtgaaaa 
      ttgtgtcgc cggaggccg catttactt gatgaagga 
      tcctctgga aacggtgcc aagagcgag aaagcggct 
      ttttgccct gtttgtgat tcactacgg atttatatt 
      tttagtttt gtatttgtt gttcaaatt tatcacttg 
      ggttatttg aaaatgaat gtagaaggt gttccaaga 
      tgcaataaa ggggctata cataaaatc gctacttat 
      ttttttata tccttactc caacatcca gtccaatat 
      ctaaataat aatttatga aaattaaaa aaaatgcag 
      atgtttatt atctttgaa aatcttgaa aattaataa 
      aagtaattt tttgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY