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Gene information
General information
TC033066 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5215543..5221398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033066
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva random shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033066 RA
      MTAPSRLDP SCTTLTKFI FAEENRIWQ STGELTRLL 
NSIQMATKS VSALVRRGG VSHLFTHAI GKQKETKRK 
AIANEIFIN MLISSYSVG VIVSEENPK PLEVDRDHR 
GKYIVAIDP LEGLPDVDC NTAIGSIFL ILDKGDDNL 
PTAQDVLFE DNQITAAGY ALYGSATTL VMSTGEGVN 
SFVLDTEIG EYIMTESNL KMPKKGNTY SINEGYTSS 
WDDLVKNYV NAKKFPHSG KPYTARYVG SMVADIHRT 
LKHGGIFMY PANKEHPHG KLKLLYECK PMAFIIEQA 
GGLSTDGTR SILDVKPRE VHQTTPIFL GSTNDVREF 
LGLASKSVR SLQ
    CDS sequence
    • >TC033066 RA
      atgactgca ccttcgcgc ctagacccc agttgtaca 
actctcaca aagtttatc ttcgccgaa gagaatcgc 
atctggcag tccactggc gaactcacg agactcctc 
aattccatt caaatggcc accaagtcg gtcagtgcc 
ttggttagg aggggcggt gtgtcccat ttgttcacc 
cacgccatc ggaaaacag aaagaaacg aaaagaaaa 
gccatcgct aacgaaatt ttcatcaac atgctcata 
tcgtcgtat tctgttggt gtgatagtg tcggaggaa 
aacccgaag cctctggaa gtcgataga gaccacaga 
ggcaagtac attgttgcc attgaccct ctggaaggt 
ctcccagac gttgactgt aacactgca atcggttcg 
attttccta atactggac aaaggcgat gacaatctg 
ccaacggct caagacgtc ctgttcgaa gacaaccaa 
atcactgct gcaggatac gcgctgtac gggagtgcc 
acaactttg gtcatgtca acaggagaa ggagtcaac 
tcttttgtc cttgatacg gaaatcgga gagtacatc 
atgaccgaa agtaatttg aagatgccg aagaaaggc 
aatacctat tccattaac gaaggttat acctcctct 
tgggacgat ttagttaag aattatgtg aatgctaag 
aaattccct cattcgggg aaaccctac actgccagg 
tatgtcggt tccatggtt gccgacata cacaggact 
ttgaaacac ggaggcatt tttatgtac ccagctaac 
aaggagcac cctcatggc aaattaaaa ttactgtac 
gagtgcaag cccatggcg ttcatcata gaacaagct 
ggtgggctt tctacggac gggacgcga agtatcctg 
gatgttaaa ccaagagag gtccatcag acaactccg 
atttttttg gggtcgacc aacgatgtc cgggaattt 
ctagggtta gcgtctaag agtgtcaga agtcttcaa 
taa
    mRNA sequence
    • >TC033066 RA
      cgccgatgc cccagcgcc ttccgctga tcacgttgc 
cacacgtgt gatacgcga tcttctgcc taagaccaa 
aaaggcaaa tttatcatc agaagactt gatagctgt 
cggcttact tttaattga tggtgtgcc aaacagccg 
agtcatcgg cagaaggtt ggagtttca cacctaccc 
ataatctaa ccgtaacca gaacaactc atcacactg 
tttggtact tatttagat cgaggaggt gatacgcac 
gggacggct cgggaatgt cgtatcatc gggagtaat 
ttcatggcg aaaaaggag cctggctag gattattgt 
tactggcga gatgcactt gcgattttt accgctgga 
cgacatctg tcacactgc atacggctt tctaacaga 
aagtgtgta atttgattg accatatta tgtttgcat 
atggtaata atgcaactt tgaaataac tgtttcgag 
acgtgtggt taaaggacg tccatgtgg cctattata 
aggctaact gaatcaaga ttccgcatt tcaaacgtc 
aaaatgact gcaccttcg cgcctagac cccagttgt 
acaactctc acaaagttt atcttcgcc gaagagaat 
cgcatctgg cagtccact ggcgaactc acgagactc 
ctcaattcc attcaaatg gccaccaag tcggtcagt 
gccttggtt aggaggggc ggtgtgtcc catttgttc 
acccacgcc atcggaaaa cagaaagaa acgaaaaga 
aaagccatc gctaacgaa attttcatc aacatgctc 
atatcgtcg tattctgtt ggtgtgata gtgtcggag 
gaaaacccg aagcctctg gaagtcgat agagaccac 
agaggcaag tacattgtt gccattgac cctctggaa 
ggtctccca gacgttgac tgtaacact gcaatcggt 
tcgattttc ctaatactg gacaaaggc gatgacaat 
ctgccaacg gctcaagac gtcctgttc gaagacaac 
caaatcact gctgcagga tacgcgctg tacgggagt 
gccacaact ttggtcatg tcaacagga gaaggagtc 
aactctttt gtccttgat acggaaatc ggagagtac 
atcatgacc gaaagtaat ttgaagatg ccgaagaaa 
ggcaatacc tattccatt aacgaaggt tatacctcc 
tcttgggac gatttagtt aagaattat gtgaatgct 
aagaaattc cctcattcg gggaaaccc tacactgcc 
aggtatgtc ggttccatg gttgccgac atacacagg 
actttgaaa cacggaggc atttttatg tacccagct 
aacaaggag caccctcat ggcaaatta aaattactg 
tacgagtgc aagcccatg gcgttcatc atagaacaa 
gctggtggg ctttctacg gacgggacg cgaagtatc 
ctggatgtt aaaccaaga gaggtccat cagacaact 
ccgattttt ttggggtcg accaacgat gtccgggaa 
tttctaggg ttagcgtct aagagtgtc agaagtctt 
caataaaac gtaacgaag ttaattcta aaagaacta 
ttttcccct aattgaaaa aatgcccaa cctagtggt 
cctgtcaat tcgtatatg gtacacatt attaacaaa 
tcgttgatt ccaaacgca ctcaaccga taaaccaca 
ctggggttt tgcactctc atcaccacc atttccaaa 
tgttcaatt aaaaacttg tccggtgt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY