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Gene information
General information
TC033066 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5215543..5221398
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033066
    iBeetle screen iB_09069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva random shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033066 RA
      MTAPSRLDP SCTTLTKFI FAEENRIWQ STGELTRLL 
      NSIQMATKS VSALVRRGG VSHLFTHAI GKQKETKRK 
      AIANEIFIN MLISSYSVG VIVSEENPK PLEVDRDHR 
      GKYIVAIDP LEGLPDVDC NTAIGSIFL ILDKGDDNL 
      PTAQDVLFE DNQITAAGY ALYGSATTL VMSTGEGVN 
      SFVLDTEIG EYIMTESNL KMPKKGNTY SINEGYTSS 
      WDDLVKNYV NAKKFPHSG KPYTARYVG SMVADIHRT 
      LKHGGIFMY PANKEHPHG KLKLLYECK PMAFIIEQA 
      GGLSTDGTR SILDVKPRE VHQTTPIFL GSTNDVREF 
      LGLASKSVR SLQ
    CDS sequence
    • >TC033066 RA
      atgactgca ccttcgcgc ctagacccc agttgtaca 
      actctcaca aagtttatc ttcgccgaa gagaatcgc 
      atctggcag tccactggc gaactcacg agactcctc 
      aattccatt caaatggcc accaagtcg gtcagtgcc 
      ttggttagg aggggcggt gtgtcccat ttgttcacc 
      cacgccatc ggaaaacag aaagaaacg aaaagaaaa 
      gccatcgct aacgaaatt ttcatcaac atgctcata 
      tcgtcgtat tctgttggt gtgatagtg tcggaggaa 
      aacccgaag cctctggaa gtcgataga gaccacaga 
      ggcaagtac attgttgcc attgaccct ctggaaggt 
      ctcccagac gttgactgt aacactgca atcggttcg 
      attttccta atactggac aaaggcgat gacaatctg 
      ccaacggct caagacgtc ctgttcgaa gacaaccaa 
      atcactgct gcaggatac gcgctgtac gggagtgcc 
      acaactttg gtcatgtca acaggagaa ggagtcaac 
      tcttttgtc cttgatacg gaaatcgga gagtacatc 
      atgaccgaa agtaatttg aagatgccg aagaaaggc 
      aatacctat tccattaac gaaggttat acctcctct 
      tgggacgat ttagttaag aattatgtg aatgctaag 
      aaattccct cattcgggg aaaccctac actgccagg 
      tatgtcggt tccatggtt gccgacata cacaggact 
      ttgaaacac ggaggcatt tttatgtac ccagctaac 
      aaggagcac cctcatggc aaattaaaa ttactgtac 
      gagtgcaag cccatggcg ttcatcata gaacaagct 
      ggtgggctt tctacggac gggacgcga agtatcctg 
      gatgttaaa ccaagagag gtccatcag acaactccg 
      atttttttg gggtcgacc aacgatgtc cgggaattt 
      ctagggtta gcgtctaag agtgtcaga agtcttcaa 
      taa
    mRNA sequence
    • >TC033066 RA
      cgccgatgc cccagcgcc ttccgctga tcacgttgc 
      cacacgtgt gatacgcga tcttctgcc taagaccaa 
      aaaggcaaa tttatcatc agaagactt gatagctgt 
      cggcttact tttaattga tggtgtgcc aaacagccg 
      agtcatcgg cagaaggtt ggagtttca cacctaccc 
      ataatctaa ccgtaacca gaacaactc atcacactg 
      tttggtact tatttagat cgaggaggt gatacgcac 
      gggacggct cgggaatgt cgtatcatc gggagtaat 
      ttcatggcg aaaaaggag cctggctag gattattgt 
      tactggcga gatgcactt gcgattttt accgctgga 
      cgacatctg tcacactgc atacggctt tctaacaga 
      aagtgtgta atttgattg accatatta tgtttgcat 
      atggtaata atgcaactt tgaaataac tgtttcgag 
      acgtgtggt taaaggacg tccatgtgg cctattata 
      aggctaact gaatcaaga ttccgcatt tcaaacgtc 
      aaaatgact gcaccttcg cgcctagac cccagttgt 
      acaactctc acaaagttt atcttcgcc gaagagaat 
      cgcatctgg cagtccact ggcgaactc acgagactc 
      ctcaattcc attcaaatg gccaccaag tcggtcagt 
      gccttggtt aggaggggc ggtgtgtcc catttgttc 
      acccacgcc atcggaaaa cagaaagaa acgaaaaga 
      aaagccatc gctaacgaa attttcatc aacatgctc 
      atatcgtcg tattctgtt ggtgtgata gtgtcggag 
      gaaaacccg aagcctctg gaagtcgat agagaccac 
      agaggcaag tacattgtt gccattgac cctctggaa 
      ggtctccca gacgttgac tgtaacact gcaatcggt 
      tcgattttc ctaatactg gacaaaggc gatgacaat 
      ctgccaacg gctcaagac gtcctgttc gaagacaac 
      caaatcact gctgcagga tacgcgctg tacgggagt 
      gccacaact ttggtcatg tcaacagga gaaggagtc 
      aactctttt gtccttgat acggaaatc ggagagtac 
      atcatgacc gaaagtaat ttgaagatg ccgaagaaa 
      ggcaatacc tattccatt aacgaaggt tatacctcc 
      tcttgggac gatttagtt aagaattat gtgaatgct 
      aagaaattc cctcattcg gggaaaccc tacactgcc 
      aggtatgtc ggttccatg gttgccgac atacacagg 
      actttgaaa cacggaggc atttttatg tacccagct 
      aacaaggag caccctcat ggcaaatta aaattactg 
      tacgagtgc aagcccatg gcgttcatc atagaacaa 
      gctggtggg ctttctacg gacgggacg cgaagtatc 
      ctggatgtt aaaccaaga gaggtccat cagacaact 
      ccgattttt ttggggtcg accaacgat gtccgggaa 
      tttctaggg ttagcgtct aagagtgtc agaagtctt 
      caataaaac gtaacgaag ttaattcta aaagaacta 
      ttttcccct aattgaaaa aatgcccaa cctagtggt 
      cctgtcaat tcgtatatg gtacacatt attaacaaa 
      tcgttgatt ccaaacgca ctcaaccga taaaccaca 
      ctggggttt tgcactctc atcaccacc atttccaaa 
      tgttcaatt aaaaacttg tccggtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY