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Gene information
General information
TC034687 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4847955..4856117
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034687
    iBeetle screen iB_09067

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09995

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034687 RA
      MIQNKSLEE TQCEENGKP KNTFSWPDY LVLGTMLLV 
      SCGIGVFYG FIGEKHSSS DDFLLGGSS MGTFPMAMS 
      LAASFITAI ELLGNPAEM YNYGTQFLM ICCAFILVV 
      PLTARFYLP LFMELRLTS SYEYFSLRF NPSVRYFAS 
      GLYILQMVL YMSVAVYAP ALALSHVTG LNVYVAVSV 
      VYIVCIFYA SQGGMKAVI IADTFQAGV LIGSILLIL 
      YLGEKFVGG TGVIWSHNY NTGRLEIFN LNPDPTIRH 
      SFWSVVIGG TFYWMTMFC SNQASIQKY LSVENINQV 
      KTALWTSCF GLILIYVIN FYTGMIMVA HYQNCDPIK 
      SGEISATDQ ILPLYVMSE MGHFKGVAG FFVAGIFAA 
      SLGTVASAL NSLAAVTIQ DFVCGAFKI VLHETKGAF 
      WAKSISAMY GAISFVLVF VVAQLGSIM QVAISFNGM 
      VGGVTLGLF SLGMFFPWA SSKGALLGS MIAVCVISV 
      MCVGQQIAI ANGDLVEES KPTTVDNCT CFNASLSHE 
      NLDGKVASS SVFFLFRIS YIWYSALGF AMTVLIGLV 
      SSLLVGCSD PHETDWNLV SPPIKNLLF SLSNKTKER 
      LNLPLKINS KKTQKNTFV GVFNMALDI NDETKPKDS 
      EKRSEKVRK ISAPSSFLV TS
    • >TC034687 RB
      MKFNGSLYS RLKLAIFLV LVISHLSEG TNNDTKQEE 
      TQCEENGKP KNTFSWPDY LVLGTMLLV SCGIGVFYG 
      FIGEKHSSS DDFLLGGSS MGTFPMAMS LAASFITAI 
      ELLGNPAEM YNYGTQFLM ICCAFILVV PLTARFYLP 
      LFMELRLTS SYEYFSLRF NPSVRYFAS GLYILQMVL 
      YMSVAVYAP ALALSHVTG LNVYVAVSV VYIVCIFYA 
      SQGGMKAVI IADTFQAGV LIGSILLIL YLGEKFVGG 
      TGVIWSHNY NTGRLEIFN LNPDPTIRH SFWSVVIGG 
      TFYWMTMFC SNQASIQKY LSVENINQV KTALWTSCF 
      GLILIYVIN FYTGMIMVA HYQNCDPIK SGEISATDQ 
      ILPLYVMSE MGHFKGVAG FFVAGIFAA SLGTVASAL 
      NSLAAVTIQ DFVCGAFKI VLHETKGAF WAKSISAMY 
      GAISFVLVF VVAQLGSIM QVAISFNGM VGGVTLGLF 
      SLGMFFPWA SSKGALLGS MIAVCVISV MCVGQQIAI 
      ANGDLVEES KPTTVDNCT CFNASLSHE NLDGKVASS 
      SVFFLFRIS YIWYSALGF AMTVLIGLV SSLLVGCSD 
      PHETDWNLV SPPIKNLLF SLSNKTKER LNLPLKINS 
      KKTQKNTFV GVFNMALDI NDETKPKDS EKRSEKVRK 
      ISAPSSFLV TS
    CDS sequence
    • >TC034687 RA
      atgatacaa aacaagtct ttagaagaa actcaatgt 
      gaagaaaac ggaaaaccc aaaaacaca ttttcctgg 
      cccgactat ttagtcctc ggcacaatg ctgctcgtt 
      tcctgcgga atcggcgtt ttttacggt ttcatcggc 
      gaaaaacac tcctccagt gacgacttc ctgctgggt 
      ggaagctca atgggcacc ttccccatg gccatgtcg 
      ctggcagcg agttttatc acagccatc gaactgcta 
      ggaaatcct gcagaaatg tacaactac gggacccag 
      tttttgatg atttgctgc gcttttatc ctggtggtg 
      ccactaacg gcccgcttt tacctccct ttgtttatg 
      gaactgcgg cttactagc agttatgaa tatttttcg 
      ctacggttt aacccttcg gttaggtat tttgcgagc 
      ggactttat atcctgcaa atggttttg tatatgagt 
      gttgcggtc tatgctccg gctttggct ctaagtcat 
      gtaaccggt ttgaacgtg tacgtggcg gtaagtgta 
      gtttacatc gtttgcata ttttacgcg tctcaggga 
      ggaatgaaa gctgttatt atcgctgat acgttccaa 
      gccggtgtt ttgatcggt tccattcta ctcatcttg 
      tatttgggc gagaaattc gttggagga accggagtt 
      atttggtcg cataattac aacaccgga cgattagaa 
      atattcaat ctgaatcct gatcccaca atacggcac 
      agcttttgg tcagtggtt attggcgga actttctac 
      tggatgacg atgttttgt tcaaatcaa gcctcgatt 
      cagaaatat ttatcagtg gaaaacatc aaccaagtt 
      aaaacggcc ttgtggacg tcatgcttc ggccttatt 
      ttaatatac gttataaat ttctacacg gggatgatc 
      atggtggca cattaccaa aactgcgac ccgatcaaa 
      tcgggcgaa atttctgca accgaccag attttgcca 
      ctttacgtc atgtcagaa atggggcat ttcaaaggc 
      gttgcagga tttttcgtt gcggggatt tttgcagcc 
      agtttaggc accgtggct tcggccctc aacagcctg 
      gcagccgtc acgatccag gatttcgtc tgcggtgct 
      tttaagatc gtcctgcac gaaaccaag ggcgccttt 
      tgggccaaa tcaatttct gcaatgtac ggggcaatt 
      agttttgtc ttggttttt gtggtggcc cagttgggg 
      agcattatg caggttgcg attagtttc aacggaatg 
      gtcgggggt gtgaccctc gggttgttc tccctgggg 
      atgtttttc ccgtgggca agctccaag ggcgcactt 
      ttggggagt atgatcgct gtgtgtgtg atcagtgtg 
      atgtgtgtt gggcaacaa attgccatc gccaatggg 
      gatttggtg gaggaatcc aaacccacc actgttgat 
      aactgtacc tgttttaac gcaagtctt tcgcacgaa 
      aacctcgat ggaaaagtg gcctcaagt tcggttttt 
      ttcctgttt cgaatatcg tacatttgg tattcggct 
      ttgggtttt gcgatgacg gtcttgatc ggcttggtg 
      tcatcgctt ctggttggg tgtagtgat ccgcatgaa 
      actgactgg aatttggtt tcgccgcct attaaaaac 
      cttttattt tccctctcc aataaaact aaagaaagg 
      ttgaatctt ccattaaaa atcaacagc aagaagacg 
      caaaaaaat acctttgtt ggcgttttt aacatggct 
      ttggacatt aacgatgaa acgaaaccg aaagacagt 
      gaaaagagg agcgaaaag gtgcgaaaa atttcagca 
      ccttcgagt tttctagtg acatcttag
    • >TC034687 RB
      atgaagttc aacggtagt ctttattct cgattaaaa 
      ctagcgata tttctcgtc ttggtgatt tcccattta 
      agtgaagga acaaacaat gatacaaaa caagaagaa 
      actcaatgt gaagaaaac ggaaaaccc aaaaacaca 
      ttttcctgg cccgactat ttagtcctc ggcacaatg 
      ctgctcgtt tcctgcgga atcggcgtt ttttacggt 
      ttcatcggc gaaaaacac tcctccagt gacgacttc 
      ctgctgggt ggaagctca atgggcacc ttccccatg 
      gccatgtcg ctggcagcg agttttatc acagccatc 
      gaactgcta ggaaatcct gcagaaatg tacaactac 
      gggacccag tttttgatg atttgctgc gcttttatc 
      ctggtggtg ccactaacg gcccgcttt tacctccct 
      ttgtttatg gaactgcgg cttactagc agttatgaa 
      tatttttcg ctacggttt aacccttcg gttaggtat 
      tttgcgagc ggactttat atcctgcaa atggttttg 
      tatatgagt gttgcggtc tatgctccg gctttggct 
      ctaagtcat gtaaccggt ttgaacgtg tacgtggcg 
      gtaagtgta gtttacatc gtttgcata ttttacgcg 
      tctcaggga ggaatgaaa gctgttatt atcgctgat 
      acgttccaa gccggtgtt ttgatcggt tccattcta 
      ctcatcttg tatttgggc gagaaattc gttggagga 
      accggagtt atttggtcg cataattac aacaccgga 
      cgattagaa atattcaat ctgaatcct gatcccaca 
      atacggcac agcttttgg tcagtggtt attggcgga 
      actttctac tggatgacg atgttttgt tcaaatcaa 
      gcctcgatt cagaaatat ttatcagtg gaaaacatc 
      aaccaagtt aaaacggcc ttgtggacg tcatgcttc 
      ggccttatt ttaatatac gttataaat ttctacacg 
      gggatgatc atggtggca cattaccaa aactgcgac 
      ccgatcaaa tcgggcgaa atttctgca accgaccag 
      attttgcca ctttacgtc atgtcagaa atggggcat 
      ttcaaaggc gttgcagga tttttcgtt gcggggatt 
      tttgcagcc agtttaggc accgtggct tcggccctc 
      aacagcctg gcagccgtc acgatccag gatttcgtc 
      tgcggtgct tttaagatc gtcctgcac gaaaccaag 
      ggcgccttt tgggccaaa tcaatttct gcaatgtac 
      ggggcaatt agttttgtc ttggttttt gtggtggcc 
      cagttgggg agcattatg caggttgcg attagtttc 
      aacggaatg gtcgggggt gtgaccctc gggttgttc 
      tccctgggg atgtttttc ccgtgggca agctccaag 
      ggcgcactt ttggggagt atgatcgct gtgtgtgtg 
      atcagtgtg atgtgtgtt gggcaacaa attgccatc 
      gccaatggg gatttggtg gaggaatcc aaacccacc 
      actgttgat aactgtacc tgttttaac gcaagtctt 
      tcgcacgaa aacctcgat ggaaaagtg gcctcaagt 
      tcggttttt ttcctgttt cgaatatcg tacatttgg 
      tattcggct ttgggtttt gcgatgacg gtcttgatc 
      ggcttggtg tcatcgctt ctggttggg tgtagtgat 
      ccgcatgaa actgactgg aatttggtt tcgccgcct 
      attaaaaac cttttattt tccctctcc aataaaact 
      aaagaaagg ttgaatctt ccattaaaa atcaacagc 
      aagaagacg caaaaaaat acctttgtt ggcgttttt 
      aacatggct ttggacatt aacgatgaa acgaaaccg 
      aaagacagt gaaaagagg agcgaaaag gtgcgaaaa 
      atttcagca ccttcgagt tttctagtg acatcttag
    mRNA sequence
    • >TC034687 RA
      gctacttta attttgatc agtgccttt cagtggttt 
      gaagtgtta aaaattttt tcgtacagt tgttcgaat 
      gaaatacag aagtcgatc taatcgaca aatcattca 
      aatccagtt tagaagttt cttgaggtg aagactgaa 
      gacagttta taacgaaat aaagttaag gttgtgcca 
      aatagtatt aaacgctaa tttattata agaagaata 
      tttattata atttatttg gaagacaaa aatgaagtt 
      caacggtag tctttattc tcgattaaa actagcgat 
      atttctcgt cttggtgat ttcccattt aagtgaagg 
      aacaaacaa tgatacaaa acaagtctt tagaagaaa 
      ctcaatgtg aagaaaacg gaaaaccca aaaacacat 
      tttcctggc ccgactatt tagtcctcg gcacaatgc 
      tgctcgttt cctgcggaa tcggcgttt tttacggtt 
      tcatcggcg aaaaacact cctccagtg acgacttcc 
      tgctgggtg gaagctcaa tgggcacct tccccatgg 
      ccatgtcgc tggcagcga gttttatca cagccatcg 
      aactgctag gaaatcctg cagaaatgt acaactacg 
      ggacccagt ttttgatga tttgctgcg cttttatcc 
      tggtggtgc cactaacgg cccgctttt acctccctt 
      tgtttatgg aactgcggc ttactagca gttatgaat 
      atttttcgc tacggttta acccttcgg ttaggtatt 
      ttgcgagcg gactttata tcctgcaaa tggttttgt 
      atatgagtg ttgcggtct atgctccgg ctttggctc 
      taagtcatg taaccggtt tgaacgtgt acgtggcgg 
      taagtgtag tttacatcg tttgcatat tttacgcgt 
      ctcagggag gaatgaaag ctgttatta tcgctgata 
      cgttccaag ccggtgttt tgatcggtt ccattctac 
      tcatcttgt atttgggcg agaaattcg ttggaggaa 
      ccggagtta tttggtcgc ataattaca acaccggac 
      gattagaaa tattcaatc tgaatcctg atcccacaa 
      tacggcaca gcttttggt cagtggtta ttggcggaa 
      ctttctact ggatgacga tgttttgtt caaatcaag 
      cctcgattc agaaatatt tatcagtgg aaaacatca 
      accaagtta aaacggcct tgtggacgt catgcttcg 
      gccttattt taatatacg ttataaatt tctacacgg 
      ggatgatca tggtggcac attaccaaa actgcgacc 
      cgatcaaat cgggcgaaa tttctgcaa ccgaccaga 
      ttttgccac tttacgtca tgtcagaaa tggggcatt 
      tcaaaggcg ttgcaggat ttttcgttg cggggattt 
      ttgcagcca gtttaggca ccgtggctt cggccctca 
      acagcctgg cagccgtca cgatccagg atttcgtct 
      gcggtgctt ttaagatcg tcctgcacg aaaccaagg 
      gcgcctttt gggccaaat caatttctg caatgtacg 
      gggcaatta gttttgtct tggtttttg tggtggccc 
      agttgggga gcattatgc aggttgcga ttagtttca 
      acggaatgg tcgggggtg tgaccctcg ggttgttct 
      ccctgggga tgtttttcc cgtgggcaa gctccaagg 
      gcgcacttt tggggagta tgatcgctg tgtgtgtga 
      tcagtgtga tgtgtgttg ggcaacaaa ttgccatcg 
      ccaatgggg atttggtgg aggaatcca aacccacca 
      ctgttgata actgtacct gttttaacg caagtcttt 
      cgcacgaaa acctcgatg gaaaagtgg cctcaagtt 
      cggtttttt tcctgtttc gaatatcgt acatttggt 
      attcggctt tgggttttg cgatgacgg tcttgatcg 
      gcttggtgt catcgcttc tggttgggt gtagtgatc 
      cgcatgaaa ctgactgga atttggttt cgccgccta 
      ttaaaaacc ttttatttt ccctctcca ataaaacta 
      aagaaaggt tgaatcttc cattaaaaa tcaacagca 
      agaagacgc aaaaaaata cctttgttg gcgttttta 
      acatggctt tggacatta acgatgaaa cgaaaccga 
      aagacagtg aaaagagga gcgaaaagg tgcgaaaaa 
      tttcagcac cttcgagtt ttctagtga catcttagc 
      accttatct ccaaactcg ctagtatta cgtttgtaa 
      cataagttc aaataagag tattatttt taataacac 
      actgttgac agtattttt gtgtgtaaa tagttaaaa 
      taagttgat tctgttgga caataattt atacataca 
      tatctagct tatttttag tgtacttac tgaaaagtt 
      ttgttcaat tgtatgaat aaagccttg aaaaaact
    • >TC034687 RB
      gctacttta attttgatc agtgccttt cagtggttt 
      gaagtgtta aaaattttt tcgtacagt tgttcgaat 
      gaaatacag aagtcgatc taatcgaca aatcattca 
      aatccagtt tagaagttt cttgaggtg aagactgaa 
      gacagttta taacgaaat aaagttaag gttgtgcca 
      aatagtatt aaacgctaa tttattata agaagaata 
      tttattata atttatttg gaagacaaa aatgaagtt 
      caacggtag tctttattc tcgattaaa actagcgat 
      atttctcgt cttggtgat ttcccattt aagtgaagg 
      aacaaacaa tgatacaaa acaagaaga aactcaatg 
      tgaagaaaa cggaaaacc caaaaacac attttcctg 
      gcccgacta tttagtcct cggcacaat gctgctcgt 
      ttcctgcgg aatcggcgt tttttacgg tttcatcgg 
      cgaaaaaca ctcctccag tgacgactt cctgctggg 
      tggaagctc aatgggcac cttccccat ggccatgtc 
      gctggcagc gagttttat cacagccat cgaactgct 
      aggaaatcc tgcagaaat gtacaacta cgggaccca 
      gtttttgat gatttgctg cgcttttat cctggtggt 
      gccactaac ggcccgctt ttacctccc tttgtttat 
      ggaactgcg gcttactag cagttatga atatttttc 
      gctacggtt taacccttc ggttaggta ttttgcgag 
      cggacttta tatcctgca aatggtttt gtatatgag 
      tgttgcggt ctatgctcc ggctttggc tctaagtca 
      tgtaaccgg tttgaacgt gtacgtggc ggtaagtgt 
      agtttacat cgtttgcat attttacgc gtctcaggg 
      aggaatgaa agctgttat tatcgctga tacgttcca 
      agccggtgt tttgatcgg ttccattct actcatctt 
      gtatttggg cgagaaatt cgttggagg aaccggagt 
      tatttggtc gcataatta caacaccgg acgattaga 
      aatattcaa tctgaatcc tgatcccac aatacggca 
      cagcttttg gtcagtggt tattggcgg aactttcta 
      ctggatgac gatgttttg ttcaaatca agcctcgat 
      tcagaaata tttatcagt ggaaaacat caaccaagt 
      taaaacggc cttgtggac gtcatgctt cggccttat 
      tttaatata cgttataaa tttctacac ggggatgat 
      catggtggc acattacca aaactgcga cccgatcaa 
      atcgggcga aatttctgc aaccgacca gattttgcc 
      actttacgt catgtcaga aatggggca tttcaaagg 
      cgttgcagg atttttcgt tgcggggat ttttgcagc 
      cagtttagg caccgtggc ttcggccct caacagcct 
      ggcagccgt cacgatcca ggatttcgt ctgcggtgc 
      ttttaagat cgtcctgca cgaaaccaa gggcgcctt 
      ttgggccaa atcaatttc tgcaatgta cggggcaat 
      tagttttgt cttggtttt tgtggtggc ccagttggg 
      gagcattat gcaggttgc gattagttt caacggaat 
      ggtcggggg tgtgaccct cgggttgtt ctccctggg 
      gatgttttt cccgtgggc aagctccaa gggcgcact 
      tttggggag tatgatcgc tgtgtgtgt gatcagtgt 
      gatgtgtgt tgggcaaca aattgccat cgccaatgg 
      ggatttggt ggaggaatc caaacccac cactgttga 
      taactgtac ctgttttaa cgcaagtct ttcgcacga 
      aaacctcga tggaaaagt ggcctcaag ttcggtttt 
      tttcctgtt tcgaatatc gtacatttg gtattcggc 
      tttgggttt tgcgatgac ggtcttgat cggcttggt 
      gtcatcgct tctggttgg gtgtagtga tccgcatga 
      aactgactg gaatttggt ttcgccgcc tattaaaaa 
      ccttttatt ttccctctc caataaaac taaagaaag 
      gttgaatct tccattaaa aatcaacag caagaagac 
      gcaaaaaaa tacctttgt tggcgtttt taacatggc 
      tttggacat taacgatga aacgaaacc gaaagacag 
      tgaaaagag gagcgaaaa ggtgcgaaa aatttcagc 
      accttcgag ttttctagt gacatctta gcaccttat 
      ctccaaact cgctagtat tacgtttgt aacataagt 
      tcaaataag agtattatt tttaataac acactgttg 
      acagtattt ttgtgtgta aatagttaa aataagttg 
      attctgttg gacaataat ttatacata catatctag 
      cttattttt agtgtactt actgaaaag ttttgttca 
      attgtatga ataaagcct tgaaaaaac t
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