Status
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Gene information
General information
TC014037 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4617472..4622253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014037
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    previtellogenic egg chamber shape irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014037 RA
      MGIFGTTTD TITDEVEKA TNENNAEEN WELIINICD 
      KAGKSAEDA KAYLRVILK RLHHQDPHI ALQAVTLLD 
      ACVKNSGKL FHIEVASRE FENEYTKLL QKSHISVSK 
      KLREALKRW AENEFKTEQ QLNLIPSLY TKLKNSGVD 
      FTFTDMPAK KSAPVSKDP NVVESQEEE NQILKAIEL 
      SLKESTSSP RASSASGSL YPSANLAAA NSSTNSTPV 
      KEPKKVRAL YDFEAAEDN ELTFKSGEI IFVIDDSDP 
      NWWKGSNQR GEGLFPSNF VTKDLDVEP EKFLYEKAK 
      KIVQFKETA DVKTGAEKT DPEDIEINE EKIDRLLHL 
      LHEADPTNP ETDTEEMLK LEREVNLMG PLIDSELER 
      VDKKHAQLT QLSADLVEA LGLYHSLMR EPQFPPKMP 
      YNYNQMAHP PPPQQPPNS MVQTYNGLP PPPPQPTSM 
      LPPGMAPPP GMHYATMPM MSTAGMPPP PPQFLMHQM 
      PDDRRTQSY GSMVLPNFP SGVAGSQTL IHNGPPPQS 
      QSQHYMPAS SANPK
    CDS sequence
    • >TC014037 RA
      atggggata ttcggtacc accaccgac accatcacg 
      gacgaagta gagaaggca acgaacgag aacaacgcc 
      gaggaaaac tgggaactg atcataaac atttgtgat 
      aaggctggc aagtctgct gaagatgcc aaggcgtac 
      ttgagggtc atcctcaaa cggctccac caccaagac 
      ccccacatc gccctccag gccgttacg ttactagac 
      gcctgtgtc aaaaacagc ggcaagttg ttccacatt 
      gaggtcgcc tcaagggag ttcgagaac gagtacacg 
      aaactactg caaaaatcg cacatcagc gtgtccaag 
      aagttgagg gaagctttg aaacggtgg gctgagaac 
      gagttcaaa accgagcaa caactcaat ctcattcct 
      tctctgtac actaaactg aagaacagc ggcgttgat 
      tttacattt acagacatg ccggccaaa aagtcggcc 
      cctgttagc aaagacccc aacgttgtc gagtcacaa 
      gaagaggag aatcagatc ctcaaagcc atagaattg 
      tccttgaaa gaaagcacg tcgtcgcca cgggcgtcc 
      agcgcaagc ggaagtctt tatccgagt gcgaatctc 
      gcagctgcg aatagttca acaaattcg actcctgtt 
      aaagaaccg aaaaaagtt agggctttg tatgatttc 
      gaagcggct gaagataat gaactgacg tttaaatcg 
      ggcgaaata atttttgtg atagatgat tctgatccg 
      aattggtgg aaagggagt aatcaaagg ggcgaaggg 
      ctgtttccg tcaaatttt gtcacgaaa gacttggac 
      gttgaacct gagaagttt ttgtacgaa aaagcgaag 
      aaaatcgtt cagttcaaa gaaaccgcg gacgtgaaa 
      accggtgcc gaaaaaacc gatcccgaa gacatagaa 
      atcaacgag gagaaaatc gaccgtctc ctccacttg 
      ctccacgaa gccgatccg acaaatccc gaaacggac 
      accgaagaa atgctaaaa ctcgaacgc gaggtgaac 
      ctaatgggt cccctaatt gattccgaa ttagaaagg 
      gtggacaag aaacacgcc caattgacc caactcagt 
      gctgatctt gttgaagct ctgggtctt tatcattct 
      ctgatgcgt gaaccacaa ttcccaccc aagatgcct 
      tacaattat aatcagatg gcacatccg ccgccacca 
      caacagccc ccaaattcg atggtgcag acgtacaac 
      ggattgccg ccgccaccg ccgcaaccc acatcaatg 
      ttgccccca ggaatggcg ccgccccca ggcatgcat 
      tacgcgacg atgcccatg atgtccacg gcgggcatg 
      ccgccgccg ccgccgcag tttttgatg catcagatg 
      ccggacgac aggaggact cagagctac gggagtatg 
      gtacttccc aattttccg agcggtgtt gccggatct 
      cagactctt atacataat ggaccgcca ccgcagtca 
      caaagtcaa cattacatg cctgcttca agcgccaat 
      ccaaagtag
    mRNA sequence
    • >TC014037 RA
      gcagcaatt gtttggtcg actgtaatg ttcatggtg 
      ctgccttca ccgacattg tcaaaactc cataaattc 
      gaaacgtca aaatttata cgtttgaaa tgacatttt 
      cttcttatc agaaacatt cagcagtgt aacaaagaa 
      ctttcacgt aattatgtc ggtttagtg tccgtttta 
      cgtgaataa atttgtaaa aaatgggga tattcggta 
      ccaccaccg acaccatca cggacgaag tagagaagg 
      caacgaacg agaacaacg ccgaggaaa actgggaac 
      tgatcataa acatttgtg ataaggctg gcaagtctg 
      ctgaagatg ccaaggcgt acttgaggg tcatcctca 
      aacggctcc accaccaag acccccaca tcgccctcc 
      aggccgtta cgttactag acgcctgtg tcaaaaaca 
      gcggcaagt tgttccaca ttgaggtcg cctcaaggg 
      agttcgaga acgagtaca cgaaactac tgcaaaaat 
      cgcacatca gcgtgtcca agaagttga gggaagctt 
      tgaaacggt gggctgaga acgagttca aaaccgagc 
      aacaactca atctcattc cttctctgt acactaaac 
      tgaagaaca gcggcgttg attttacat ttacagaca 
      tgccggcca aaaagtcgg cccctgtta gcaaagacc 
      ccaacgttg tcgagtcac aagaagagg agaatcaga 
      tcctcaaag ccatagaat tgtccttga aagaaagca 
      cgtcgtcgc cacgggcgt ccagcgcaa gcggaagtc 
      tttatccga gtgcgaatc tcgcagctg cgaatagtt 
      caacaaatt cgactcctg ttaaagaac cgaaaaaag 
      ttagggctt tgtatgatt tcgaagcgg ctgaagata 
      atgaactga cgtttaaat cgggcgaaa taatttttg 
      tgatagatg attctgatc cgaattggt ggaaaggga 
      gtaatcaaa ggggcgaag ggctgtttc cgtcaaatt 
      ttgtcacga aagacttgg acgttgaac ctgagaagt 
      ttttgtacg aaaaagcga agaaaatcg ttcagttca 
      aagaaaccg cggacgtga aaaccggtg ccgaaaaaa 
      ccgatcccg aagacatag aaatcaacg aggagaaaa 
      tcgaccgtc tcctccact tgctccacg aagccgatc 
      cgacaaatc ccgaaacgg acaccgaag aaatgctaa 
      aactcgaac gcgaggtga acctaatgg gtcccctaa 
      ttgattccg aattagaaa gggtggaca agaaacacg 
      cccaattga cccaactca gtgctgatc ttgttgaag 
      ctctgggtc tttatcatt ctctgatgc gtgaaccac 
      aattcccac ccaagatgc cttacaatt ataatcaga 
      tggcacatc cgccgccac cacaacagc ccccaaatt 
      cgatggtgc agacgtaca acggattgc cgccgccac 
      cgccgcaac ccacatcaa tgttgcccc caggaatgg 
      cgccgcccc caggcatgc attacgcga cgatgccca 
      tgatgtcca cggcgggca tgccgccgc cgccgccgc 
      agtttttga tgcatcaga tgccggacg acaggagga 
      ctcagagct acgggagta tggtacttc ccaattttc 
      cgagcggtg ttgccggat ctcagactc ttatacata 
      atggaccgc caccgcagt cacaaagtc aacattaca 
      tgcctgctt caagcgcca atccaaagt agtaggcct 
      tgaaagaag tattgttat caaattctt ttccttata 
      accgatatg tgatttaac aatttagtt attttgata 
      atatcatgt attttatta cgaatttct tcactatct 
      ttgttgttt tagtttatt tattaatga gaaaattga 
      tattaagtt acaatggca ggcgtgtgg tggggaaag 
      gtagtgaag aaattatta taagtaatg ttgtagact 
      tgatgtaaa aacatgtat atgaataaa ttttatgaa 
      gaaat
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