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Gene information
General information
TC014029 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4484550..4489186
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014029
    iBeetle screen iB_09064

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014029 RA
      MVLALVNFT RYFPLRHIN RYVCAMNQA YLRHLTESD 
      RFELTFQYV DPTGRINRQ FNFCRQITE SATTFTQRV 
      ATNIEKIVK KKAKNAPTS SPVQVSLTV NGSPVSDET 
      TLCKDIFSE QNSVVLSVC EQEFKVVIN SPWISGVVL 
      PSSILAGFP VYPTKFESI YTDKTKSEF NWFKSSDKN 
      KWSLVGTGF VYNTNNSDI GAYLKLSCV PKSGDLVGP 
      AAEAISPVQ VQACPGFCP FESRHQFTK EKCSGDTFR 
      VVSYNILAD LYCDSDFTR EVLHPYCPP YALAIDYRK 
      QLFIKEITG YNGDLICLQ EVDRKIYNY DLQPLFEQL 
      GYDSDFCIK RGSVAEGLA CFYNRERFK CLETFRLVL 
      SDELNTNSL FSDIWAKIE GNKNLTERI LNRSTVLQV 
      NILESLEND EVLVVGNTH LYFHPDADH IRLLQGAAI 
      IRYLEHLMD EFRNKYKKR LSLILCGDF NSTPECGIY 
      QLYTTGHVP EDHIDFKSN AEEAIKVPL SQSLRLFSA 
      CGTPKYTNF TAGFADCLD YIYFESKNF EVVQVVPFP 
      SLEELNQHT ALPSIVFPS DHISLISDL KWKK
    CDS sequence
    • >TC014029 RA
      atggttttg gcccttgtc aatttcaca cgttacttt 
      ccgctgcgc cacatcaac cgctacgtc tgtgcgatg 
      aaccaagcc tatttgaga cacttaacc gagtcagac 
      aggttcgag ctgactttc cagtacgta gaccccaca 
      ggtcgtatc aaccgccag tttaatttc tgtcgccaa 
      atcaccgaa tctgccact accttcacc caacgtgtg 
      gccacaaac atcgaaaaa atcgtcaag aaaaaagca 
      aaaaacgcc cccacaagt tcccccgtt caagtctca 
      ctcacagtg aacggaagt ccggtatcc gacgaaacg 
      acgctttgc aaggatatt tttagtgag caaaacagc 
      gtcgttttg agcgtgtgt gagcaggag tttaaagtg 
      gtgataaat tcgccttgg atcagcggt gtggtgctc 
      cctagctcg attctggcc ggctttccg gtctacccc 
      acgaagttt gaaagcatt tacaccgat aaaaccaaa 
      tccgagttt aactggttc aaatcgagt gataaaaat 
      aagtggagt ttagtgggg accggtttt gtgtacaac 
      acaaacaat agcgacatt ggggcctat ttgaaactg 
      agctgtgtg ccgaaaagc ggtgacttg gtggggccc 
      gcggctgag gccatttca cctgtgcaa gtccaagcc 
      tgtcctgga ttttgtcca tttgaatcg agacaccaa 
      ttcaccaaa gaaaaatgt tcaggggat acgtttaga 
      gtagtgtcc tataatatt ttggcagat ttatattgc 
      gattccgat ttcacgaga gaggttttg cacccttat 
      tgcccgcct tacgccctt gctattgat tacagaaag 
      cagttgttt atcaaagaa ataactggg tataacggt 
      gatttaatt tgcttgcaa gaagtcgat cggaaaatc 
      tacaactac gacctgcag ccccttttc gagcaattg 
      ggttacgat tctgatttt tgcataaaa aggggttca 
      gtggccgaa ggactggct tgtttctac aatagggag 
      aggtttaag tgtttggag acgtttcgt ttagttttg 
      tcggatgaa ttgaatacg aattcgttg ttttcggat 
      atttgggca aaaatcgaa ggaaataaa aatttgact 
      gaacgcatt ttgaatagg tctactgtt cttcaagta 
      aatattctt gaatcgctg gaaaatgat gaggttttg 
      gtggttgga aacacgcat ttgtatttc catccagac 
      gccgatcat atcaggcta ttacaaggg gctgcgatt 
      atcagatat ttggaacat ttgatggac gagtttagg 
      aataaatat aagaagcga ctctctttg atactttgt 
      ggcgacttc aacagcacc cctgagtgt ggcatttac 
      cagttatac accactgga cacgtcccc gaagaccac 
      atcgacttc aaaagcaat gccgaagag gccatcaag 
      gtgcccctc agccagagc ttgcggctg ttcagcgct 
      tgtgggact ccaaaatac accaatttc acggcgggt 
      ttcgccgat tgcctcgac tacatttac ttcgaatcg 
      aaaaatttc gaagtggtc caagtggtg ccattcccc 
      tccttggag gaactgaac caacacacg gctctcccc 
      agcatcgtg ttcccatcc gaccacatt tcgctaatc 
      tctgattta aagtggaag aaataa
    mRNA sequence
    • >TC014029 RA
      cttttttcg tacctaacc tcaataatt tttttaaga 
      aacactttt taggggccc tttccaatg gttttggcc 
      cttgtcaat ttcacacgt tactttccg ctgcgccac 
      atcaaccgc tacgtctgt gcgatgaac caagcctat 
      ttgagacac ttaaccgag tcagacagg ttcgagctg 
      actttccag tacgtagac cccacaggt cgtatcaac 
      cgccagttt aatttctgt cgccaaatc accgaatct 
      gccactacc ttcacccaa cgtgtggcc acaaacatc 
      gaaaaaatc gtcaagaaa aaagcaaaa aacgccccc 
      acaagttcc cccgttcaa gtctcactc acagtgaac 
      ggaagtccg gtatccgac gaaacgacg ctttgcaag 
      gatattttt agtgagcaa aacagcgtc gttttgagc 
      gtgtgtgag caggagttt aaagtggtg ataaattcg 
      ccttggatc agcggtgtg gtgctccct agctcgatt 
      ctggccggc tttccggtc taccccacg aagtttgaa 
      agcatttac accgataaa accaaatcc gagtttaac 
      tggttcaaa tcgagtgat aaaaataag tggagttta 
      gtggggacc ggttttgtg tacaacaca aacaatagc 
      gacattggg gcctatttg aaactgagc tgtgtgccg 
      aaaagcggt gacttggtg gggcccgcg gctgaggcc 
      atttcacct gtgcaagtc caagcctgt cctggattt 
      tgtccattt gaatcgaga caccaattc accaaagaa 
      aaatgttca ggggatacg tttagagta gtgtcctat 
      aatattttg gcagattta tattgcgat tccgatttc 
      acgagagag gttttgcac ccttattgc ccgccttac 
      gcccttgct attgattac agaaagcag ttgtttatc 
      aaagaaata actgggtat aacggtgat ttaatttgc 
      ttgcaagaa gtcgatcgg aaaatctac aactacgac 
      ctgcagccc cttttcgag caattgggt tacgattct 
      gatttttgc ataaaaagg ggttcagtg gccgaagga 
      ctggcttgt ttctacaat agggagagg tttaagtgt 
      ttggagacg tttcgttta gttttgtcg gatgaattg 
      aatacgaat tcgttgttt tcggatatt tgggcaaaa 
      atcgaagga aataaaaat ttgactgaa cgcattttg 
      aataggtct actgttctt caagtaaat attcttgaa 
      tcgctggaa aatgatgag gttttggtg gttggaaac 
      acgcatttg tatttccat ccagacgcc gatcatatc 
      aggctatta caaggggct gcgattatc agatatttg 
      gaacatttg atggacgag tttaggaat aaatataag 
      aagcgactc tctttgata ctttgtggc gacttcaac 
      agcacccct gagtgtggc atttaccag ttatacacc 
      actggacac gtccccgaa gaccacatc gacttcaaa 
      agcaatgcc gaagaggcc atcaaggtg cccctcagc 
      cagagcttg cggctgttc agcgcttgt gggactcca 
      aaatacacc aatttcacg gcgggtttc gccgattgc 
      ctcgactac atttacttc gaatcgaaa aatttcgaa 
      gtggtccaa gtggtgcca ttcccctcc ttggaggaa 
      ctgaaccaa cacacggct ctccccagc atcgtgttc 
      ccatccgac cacatttcg ctaatctct gatttaaag 
      tggaagaaa taaaataaa aaataatcg ggcag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY