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Gene information
General information
TC013786 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 4093361..4096445
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013786
    iBeetle screen iB_09061

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013786 RA
      MPAKYFRDK NFIYCIRNK KRVYYPVCV 
      QKRKEYVKY AKVFPFSEA LDFKIPSKW 
      YRRTVGTLE IVCGIAMAF VPNHKVKNG 
      ANLCLLLLT LMAVYSHYM VADKLERTA 
      PALVFLFML SGRLVVYYQ LTRREAERP 
      EATVNGFKQ E
    • >TC013786 RB
      MGSIVLKSL SILLGLFFI FVGFIKLSS 
      YISKDLHKD LRKEYVKYA KVFPFSEAL 
      DFKIPSKWY RRTVGTLEI VCGIAMAFV 
      PNHKVKNGA NLCLLLLTL MAVYSHYMV 
      ADKLERTAP ALVFLFMLS GRLVVYYQL 
      TRREAERPE ATVNGFKQE
    CDS sequence
    • >TC013786 RA
      atgcctgcc aaatatttt cgcgacaaa aatttcatt 
      tactgcatt cgcaataaa aagcgagtt tattatcct 
      gtttgcgtg caaaagcgg aaagagtac gtcaagtac 
      gccaaagtc ttcccgttt tcggaagca ctggacttc 
      aaaatcccg tcgaaatgg taccgaagg acggtgggc 
      actttggag atcgtttgc gggatcgca atggccttc 
      gtgccgaac cataaagtg aagaatggg gcgaacttg 
      tgcttgctg ctgctcacc ctcatggcc gtctattcc 
      cactacatg gtggccgat aaactggag aggacagca 
      ccagcccta gtatttttg tttatgtta agtggcaga 
      cttgttgtt tactaccag cttacaagg agagaagcc 
      gaaagacca gaagcgacc gtcaatggg ttcaagcag 
      gaataa
    • >TC013786 RB
      atgggatca atcgtttta aaatccctc tcaatacta 
      ctaggactg tttttcatt ttcgtgggc tttatcaaa 
      ttgtccagc tacatctcc aaggacctg cacaaagac 
      ttgcggaaa gagtacgtc aagtacgcc aaagtcttc 
      ccgttttcg gaagcactg gacttcaaa atcccgtcg 
      aaatggtac cgaaggacg gtgggcact ttggagatc 
      gtttgcggg atcgcaatg gccttcgtg ccgaaccat 
      aaagtgaag aatggggcg aacttgtgc ttgctgctg 
      ctcaccctc atggccgtc tattcccac tacatggtg 
      gccgataaa ctggagagg acagcacca gccctagta 
      tttttgttt atgttaagt ggcagactt gttgtttac 
      taccagctt acaaggaga gaagccgaa agaccagaa 
      gcgaccgtc aatgggttc aagcaggaa taa
    mRNA sequence
    • >TC013786 RA
      aggcatgag agcgtcgcc gtcgtttaa gatggcaag 
      atcgtgccc ccagaatca acaccaaac taattaaca 
      acaaataaa caacaatgt ttatacact agtgacatt 
      ttccagcgc gaaccagtg atatagtta aaacaaaca 
      atgggatca atcgtttta aaatccctc tcaatacta 
      ctaggactg tttttcatt ttcgtgggc tttatcaaa 
      ttgtccagc tacatctcc aaggacctg cacaaagac 
      ttggtaagt cgccgtgtt acacaatcc gatatttta 
      aatttttgg cccgatgcc tgccaaata ttttcgcga 
      caaaaattt catttactg cattcgcaa taaaaagcg 
      agtttatta tcctgtttg cgtgcaaaa gcggaaaga 
      gtacgtcaa gtacgccaa agtcttccc gttttcgga 
      agcactgga cttcaaaat cccgtcgaa atggtaccg 
      aaggacggt gggcacttt ggagatcgt ttgcgggat 
      cgcaatggc cttcgtgcc gaaccataa agtgaagaa 
      tggggcgaa cttgtgctt gctgctgct caccctcat 
      ggccgtcta ttcccacta catggtggc cgataaact 
      ggagaggac agcaccagc cctagtatt tttgtttat 
      gttaagtgg cagacttgt tgtttacta ccagcttac 
      aaggagaga agccgaaag accagaagc gaccgtcaa 
      tgggttcaa gcaggaata aatagtgca aaactctca 
      aatcgcgtc caaaaacga gtccacctt attacgtgt 
      acctatagt tatttgtgg ttttggaca cacgaaatt 
      gtcttggtt gtttattat ttagactgc tagcatata 
      attttcaat gttggttta tgcaaataa ataacaaaa 
      ctgcgttat cattagtta ttgttgtcg ttgtctgat 
      gtacaaatt gtgaaatat ctattatta gacgtaaat 
      acacatttt tatctcac
    • >TC013786 RB
      aggcatgag agcgtcgcc gtcgtttaa gatggcaag 
      atcgtgccc ccagaatca acaccaaac taattaaca 
      acaaataaa caacaatgt ttatacact agtgacatt 
      ttccagcgc gaaccagtg atatagtta aaacaaaca 
      atgggatca atcgtttta aaatccctc tcaatacta 
      ctaggactg tttttcatt ttcgtgggc tttatcaaa 
      ttgtccagc tacatctcc aaggacctg cacaaagac 
      ttgcggaaa gagtacgtc aagtacgcc aaagtcttc 
      ccgttttcg gaagcactg gacttcaaa atcccgtcg 
      aaatggtac cgaaggacg gtgggcact ttggagatc 
      gtttgcggg atcgcaatg gccttcgtg ccgaaccat 
      aaagtgaag aatggggcg aacttgtgc ttgctgctg 
      ctcaccctc atggccgtc tattcccac tacatggtg 
      gccgataaa ctggagagg acagcacca gccctagta 
      tttttgttt atgttaagt ggcagactt gttgtttac 
      taccagctt acaaggaga gaagccgaa agaccagaa 
      gcgaccgtc aatgggttc aagcaggaa taaatagtg 
      caaaactct caaatcgcg tccaaaaac gagtccacc 
      ttattacgt gtacctata gttatttgt ggttttgga 
      cacacgaaa ttgtcttgg ttgtttatt atttagact 
      gctagcata taattttca atgttggtt tatgcaaat 
      aaataacaa aactgcgtt atcattagt tattgttgt 
      cgttgtctg atgtacaaa ttgtgaaat atctattat 
      tagacgtaa atacacatt tttatctca c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY