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Gene information
General information
TC013829 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 3549510..3550807
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013829
    iBeetle screen iB_09055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum complete not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    antenna shape bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    vertex triplet vertex triplet anterior complete not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013829 RA
      MVRLPMSLS RGPQILSLV NKTPVFLRL 
      FATQIASFD EVKSVKTTP KTLLIDVRE 
      PHELQETGV IPDSINIPL GEVEKVLKD 
      MPSEQFQKT YKKAKPDSS TPLIFSCRS 
      GKRSALASE IATKLGFKD IKNYSGGWL 
      EWEEKSKNI
    • >TC013829 RB
      MSLSRGPQI LSLVNKTPV FLRLFATQI 
      ASFDEVKSV KTTPKTLLI DVREPHELQ 
      ETGVIPDSI NIPLGEVEK VLKDMPSEQ 
      FQKTYKKAK PDSSTPLIF SCRSGKRSA 
      LASEIATKL GFKDIKNYS GGWLEWEEK 
      SKNI
    CDS sequence
    • >TC013829 RA
      atggttagg ttaccaatg tcgctatca agggggccc 
      caaatcctc tcccttgtg aacaaaacg cccgtcttt 
      cttagactt ttcgcaacg caaatcgcc tcatttgat 
      gaagtcaag tcagtaaaa acgactcct aagactctt 
      ctaatcgat gtcagggag ccccacgaa ttgcaggaa 
      accggagtt attcctgac agcatcaat attccactt 
      ggcgaagtc gagaaagtg ttaaaagac atgccttct 
      gaacaattt caaaaaact tacaaaaag gctaaacct 
      gattctagc accccctta attttttcg tgtcgttcg 
      gggaaacgt agtgccctt gcgtccgag attgcgacg 
      aagttgggg ttcaaagat ataaagaat tattccggg 
      gggtggcta gagtgggag gaaaaaagt aaaaatatt 
      tga
    • >TC013829 RB
      atgtcgcta tcaaggggg ccccaaatc ctctccctt 
      gtgaacaaa acgcccgtc tttcttaga cttttcgca 
      acgcaaatc gcctcattt gatgaagtc aagtcagta 
      aaaacgact cctaagact cttctaatc gatgtcagg 
      gagccccac gaattgcag gaaaccgga gttattcct 
      gacagcatc aatattcca cttggcgaa gtcgagaaa 
      gtgttaaaa gacatgcct tctgaacaa tttcaaaaa 
      acttacaaa aaggctaaa cctgattct agcaccccc 
      ttaattttt tcgtgtcgt tcggggaaa cgtagtgcc 
      cttgcgtcc gagattgcg acgaagttg gggttcaaa 
      gatataaag aattattcc ggggggtgg ctagagtgg 
      gaggaaaaa agtaaaaat atttga
    mRNA sequence
    • >TC013829 RA
      agtcagcgg cgtcttctt cggttaagt cggtgtttg 
      cgtttgcca ttttttcga aaactgttt ggataccac 
      aaaaactta ggttatgtg cataggtta tgtgagcgg 
      caagcgctg cctacctgg caaaattta aagcactag 
      cactttact agtgcggtg ttttttgac gattattta 
      tgaaattat tatgtaatt cgaacgcaa tcggcataa 
      aacgtgaaa aaaaaaata attggggaa aatttgatt 
      gtagccacg ggacctcta atttgtttt gtgtgaatt 
      gtgtgatac caacggtga ctgtaaaaa gtctccaaa 
      ctgattttt gccaaaact gtcgtaatt taataaaac 
      aggcgataa aaaatagta aaaatcgct ttattggcg 
      ctatggtta ggttaccaa tgtcgctat caagggggc 
      cccaaatcc tctcccttg tgaacaaaa cgcccgtct 
      ttcttagac ttttcgcaa cgcaaatcg cctcatttg 
      atgaagtca agtcagtaa aaacgactc ctaagactc 
      ttctaatcg atgtcaggg agccccacg aattgcagg 
      aaaccggag ttattcctg acagcatca atattccac 
      ttggcgaag tcgagaaag tgttaaaag acatgcctt 
      ctgaacaat ttcaaaaaa cttacaaaa aggctaaac 
      ctgattcta gcaccccct taatttttt cgtgtcgtt 
      cggggaaac gtagtgccc ttgcgtccg agattgcga 
      cgaagttgg ggttcaaag atataaaga attattccg 
      gggggtggc tagagtggg aggaaaaaa gtaaaaata 
      tttgagttg taaataaat aatacgtta tatgagtgt 
      aggggttgt agtttgatt tggatttag gtagtgccg 
      tatttactt atttactgc ggcacaaaa gtgataaac 
      atggaatgt taaaaaata agtcaaaaa gagtttgca 
      atctacaaa tctcgtcaa aaatttttt gaatgataa 
      caccttttg aaaggcctg atcaccata agtcaaaaa 
      tactcacaa aagcaaaac caaataata attttgcat 
      gtgttttgc cctgaagtg gatattaga tacgatttc 
      ttcatttgt tgatgttag gagcatcca gcgccaatg 
      tggacacaa acacttatt gcaaaaaaa catcagcgc 
      cgttttgaa aacaacatt tacctactc catgacccc 
      cttccgtac ttttaataa c
    • >TC013829 RB
      agtcagcgg cgtcttctt cggttaagt cggtgtttg 
      cgtttgcca ttttttcga aaactgttt ggataccac 
      aaaaactta ggttatgtg cataggtta tgtgagcgg 
      caagcgctg cctacctgg caaaattta aagcactag 
      cactttact agtgcggtg ttttttgac gattattta 
      tgaaattat tatgtaatt cgaacgcaa tcggcataa 
      aacgtgaaa aaaaaaata attggggaa aatttgatt 
      gtagccacg ggacctcta atttgtttt gtgtgaatt 
      gtgtgatac caacggtga ctgtaaaaa gtctccaaa 
      ctgattttt gccaaaact gtcgtaatt taataaaac 
      aggcgataa aaaatagta aaaatcgct ttattggcg 
      ctatggtta ggttaccaa tgtcgctat caagggggc 
      cccaaatcc tctcccttg tgaacaaaa cgcccgtct 
      ttcttagac ttttcgcaa cgcaaatcg cctcatttg 
      atgaagtca agtcagtaa aaacgactc ctaagactc 
      ttctaatcg atgtcaggg agccccacg aattgcagg 
      aaaccggag ttattcctg acagcatca atattccac 
      ttggcgaag tcgagaaag tgttaaaag acatgcctt 
      ctgaacaat ttcaaaaaa cttacaaaa aggctaaac 
      ctgattcta gcaccccct taatttttt cgtgtcgtt 
      cggggaaac gtagtgccc ttgcgtccg agattgcga 
      cgaagttgg ggttcaaag atataaaga attattccg 
      gggggtggc tagagtggg aggaaaaaa gtaaaaata 
      tttgagttg taaataaat aatacgtta tatgagtgt 
      aggggttgt agtttgatt tggatttag gtagtgccg 
      tatttactt atttactgc ggcacaaaa gtgataaac 
      atggaatgt taaaaaata agtcaaaaa gagtttgca 
      atctacaaa tctcgtcaa aaatttttt gaatgataa 
      caccttttg aaaggcctg atcaccata agtcaaaaa 
      tactcacaa aagcaaaac caaataata attttgcat 
      gtgttttgc cctgaagtg gatattaga tacgatttc 
      ttcatttgt tgatgttag gagcatcca gcgccaatg 
      tggacacaa acacttatt gcaaaaaaa catcagcgc 
      cgttttgaa aacaacatt tacctactc catgacccc 
      cttccgtac ttttaataa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY