Status
Please wait ...
Gene information
General information
TC013866 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2678257..2684287
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013866
    iBeetle screen iB_09053

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013866 RA
      MSRDPENPD ATIPPPPPP PMPNFGRGP GRMGYRRLE 
      EDDELLMPR PSILLPHLQ ESEKRFFEL VHSGDVAAV 
      NDFLNENPG FNINCVNFQ GVSGLLIAV QSRSEAMVE 
      FLLSQPDID IGDCVLHAI RDNQPKILE LLLEKQRNT 
      APSLEYVGV THSSDFPDY VTPLILAAQ CGHYEIIEM 
      LIDRGHTIS KPHSPSCRC MDCKAQLER DDLLHAEHL 
      RLNLYKSVC NPAYICHSS RDPILTSFQ LSTELRQCS 
      FLVPEFRNS YLELANEVS NFAVELIAG CRNSGEVET 
      ILKQKAGIQ NASIFMYPR LVLAMDYKQ KFFVAHAHV 
      QHIVESLWR GNWYDYNLK PIPAKIIYP IFRILLLPI 
      IVVMCIFLP KHQMVAHWN IPLNRMINH VAAFLVFLV 
      IIFLESNRS KENQKRMPP NSGLEPVII IFVVANCWS 
      VVRMCLIQG PRRYFTYLW NWHAVISNT FFVLTFVFW 
      LASYVDAVN NDQVDLERK YWHHLDPVL IAEGTFAVA 
      VIMTYFKLM FFCRLNYYL GPLQISLGK MCSDMAKYI 
      TIFIIIIIS FTAGLCRFY SYYDGMVQV DSNGIKTAQ 
      VSSFIDFST TLKTFFWAV FCMAPLETG DVIIENLPG 
      DTENTTIIN KHLFTEAVG YIAFALFEV LTVVMILNM 
      LIATMSSTF QRVLDNIDV EWTFGKTDF YLEYMLQPT 
      LPPPLNLIP TPSGISAFM EWFQSPKVD PREEEKRND 
      YPALMSQLV QRYFRDKDS AASPENDLE FLKQEIAEI 
      KQAVNDLLE KE
    CDS sequence
    • >TC013866 RA
      atgtctcga gaccccgaa aaccccgat gccaccata 
      ccgccccct ccgccgccc ccaatgccc aatttcggc 
      cgtggtccc ggccggatg ggataccgg aggctggag 
      gaagacgac gaattgcta atgcctcgc cctagcatc 
      ctcctgcct cacctccaa gagtctgaa aagcgtttt 
      ttcgagcta gttcactca ggtgacgtc gccgctgtt 
      aacgacttt ctcaacgaa aatcccggt tttaatatt 
      aattgcgtc aatttccaa ggagtgtcg ggtttacta 
      atcgcggtt caaagccgc tctgaggcc atggttgag 
      tttttgtta tcacagccc gatattgat attggagat 
      tgtgtcctg catgcaatt agggacaac caacccaaa 
      atcctcgaa cttcttttg gaaaaacag agaaacacg 
      gctccgagt ctggagtac gtgggggtc actcacagc 
      tcggatttt cccgattat gtgacgcct ttgattctg 
      gcggcgcag tgtggtcac tacgagatc atcgaaatg 
      ttgattgat cgggggcac accatcagc aagccccat 
      tcgccctcc tgtaggtgc atggactgc aaggcgcag 
      ctcgaacgc gacgacctc ctccatgcc gaacacctc 
      cgcctcaac ctgtacaaa tcggtgtgc aaccctgcc 
      tacatctgc cactcctca cgcgacccc atcctcacc 
      agcttccaa ctctcaacc gaactacgt cagtgctcc 
      ttcctagtc ccggaattc cgcaacagc tacctcgaa 
      ctggccaac gaagtgagc aatttcgca gtggaactc 
      atcgccggt tgtcgcaac tcaggcgaa gtcgaaacg 
      atcctaaag cagaaagct ggcattcag aacgccagc 
      attttcatg taccctcga ctagtccta gcgatggac 
      tacaagcaa aaattcttc gtggcacat gcccacgtc 
      cagcacatt gttgagtcc ctctggcgg ggcaactgg 
      tacgactac aacctcaag ccgatcccg gcgaagatc 
      atctacccc atttttcgg attctgctc cttccgatc 
      attgtagtg atgtgtatt tttttgccg aagcaccag 
      atggtggcc cattggaac atcccgctc aatcggatg 
      attaaccat gtggcggct tttttggtc ttcttggtg 
      attattttt ctagagtcg aataggtcc aaagagaat 
      cagaagcga atgcctcca aattcgggg ctcgagccc 
      gtgattata atcttcgtg gtggcgaat tgttggagt 
      gttgtcagg atgtgtctc attcagggg ccccggagg 
      tactttacg tacctctgg aactggcat gcggtcatt 
      tctaacact ttcttcgtc cttactttc gttttctgg 
      ttggcttcg tacgtcgat gcggtcaat aacgaccaa 
      gtggacttg gagcggaag tattggcac catctcgac 
      ccggttttg atcgcggaa ggtactttt gcagttgcc 
      gtcatcatg acctatttt aagctgatg tttttttgc 
      cgactgaac tactattta ggacctttg caaatttct 
      ttgggcaaa atgtgctcc gacatggcc aaatacatc 
      acaatcttc atcattatc ataatttcg ttcaccgct 
      gggttgtgc cgattttac agttactac gatggtatg 
      gtccaagtt gactcaaac gggatcaaa acggctcaa 
      gtctcctca ttcatcgac tttagcaca actttaaaa 
      acttttttc tgggctgtt ttctgcatg gcccctctt 
      gaaactggc gatgtgatt atcgagaac cttccaggg 
      gataccgaa aacaccaca attatcaac aagcacctc 
      tttaccgaa gcagtgggt tatatcgcg tttgccctc 
      ttcgaagtg ctcacagtt gtgatgatt ctcaacatg 
      ttaattgcg acgatgtcg tcgacgttt caacgagtc 
      ctcgacaat atcgacgtc gaatggacg tttggaaaa 
      accgatttt tatctagag tatatgcta caacccaca 
      cttccacca ccgttgaat ttgattcca actcctagc 
      ggaatttcg gcgtttatg gagtggttt caatcgcca 
      aaagttgat ccgcgagaa gaagaaaag agaaatgat 
      tatcctgcg ctcatgtcg cagttggtg caacgatat 
      tttagggac aaagattcg gccgcgagt cctgaaaac 
      gacttggag ttcctcaag caggaaatc gcagaaatt 
      aagcaagcg gttaatgac ctattagag aaggagtaa
    mRNA sequence
    • >TC013866 RA
      catttttgt accgtcatt ttaagttta caccccttt 
      ttattttta attttattc cccaataaa tgtctcgag 
      accccgaaa accccgatg ccaccatac cgccccctc 
      cgccgcccc caatgccca atttcggcc gtggtcccg 
      gccggatgg gataccgga ggctggagg aagacgacg 
      aattgctaa tgcctcgcc ctagcatcc tcctgcctc 
      acctccaag agtctgaaa agcgttttt tcgagctag 
      ttcactcag gtgacgtcg ccgctgtta acgactttc 
      tcaacgaaa atcccggtt ttaatatta attgcgtca 
      atttccaag gagtgtcgg gtttactaa tcgcggttc 
      aaagccgct ctgaggcca tggttgagt ttttgttat 
      cacagcccg atattgata ttggagatt gtgtcctgc 
      atgcaatta gggacaacc aacccaaaa tcctcgaac 
      ttcttttgg aaaaacaga gaaacacgg ctccgagtc 
      tggagtacg tgggggtca ctcacagct cggattttc 
      ccgattatg tgacgcctt tgattctgg cggcgcagt 
      gtggtcact acgagatca tcgaaatgt tgattgatc 
      gggggcaca ccatcagca agccccatt cgccctcct 
      gtaggtgca tggactgca aggcgcagc tcgaacgcg 
      acgacctcc tccatgccg aacacctcc gcctcaacc 
      tgtacaaat cggtgtgca accctgcct acatctgcc 
      actcctcac gcgacccca tcctcacca gcttccaac 
      tctcaaccg aactacgtc agtgctcct tcctagtcc 
      cggaattcc gcaacagct acctcgaac tggccaacg 
      aagtgagca atttcgcag tggaactca tcgccggtt 
      gtcgcaact caggcgaag tcgaaacga tcctaaagc 
      agaaagctg gcattcaga acgccagca ttttcatgt 
      accctcgac tagtcctag cgatggact acaagcaaa 
      aattcttcg tggcacatg cccacgtcc agcacattg 
      ttgagtccc tctggcggg gcaactggt acgactaca 
      acctcaagc cgatcccgg cgaagatca tctacccca 
      tttttcgga ttctgctcc ttccgatca ttgtagtga 
      tgtgtattt ttttgccga agcaccaga tggtggccc 
      attggaaca tcccgctca atcggatga ttaaccatg 
      tggcggctt ttttggtct tcttggtga ttatttttc 
      tagagtcga ataggtcca aagagaatc agaagcgaa 
      tgcctccaa attcggggc tcgagcccg tgattataa 
      tcttcgtgg tggcgaatt gttggagtg ttgtcagga 
      tgtgtctca ttcaggggc cccggaggt actttacgt 
      acctctgga actggcatg cggtcattt ctaacactt 
      tcttcgtcc ttactttcg ttttctggt tggcttcgt 
      acgtcgatg cggtcaata acgaccaag tggacttgg 
      agcggaagt attggcacc atctcgacc cggttttga 
      tcgcggaag gtacttttg cagttgccg tcatcatga 
      cctatttta agctgatgt ttttttgcc gactgaact 
      actatttag gacctttgc aaatttctt tgggcaaaa 
      tgtgctccg acatggcca aatacatca caatcttca 
      tcattatca taatttcgt tcaccgctg ggttgtgcc 
      gattttaca gttactacg atggtatgg tccaagttg 
      actcaaacg ggatcaaaa cggctcaag tctcctcat 
      tcatcgact ttagcacaa ctttaaaaa cttttttct 
      gggctgttt tctgcatgg cccctcttg aaactggcg 
      atgtgatta tcgagaacc ttccagggg ataccgaaa 
      acaccacaa ttatcaaca agcacctct ttaccgaag 
      cagtgggtt atatcgcgt ttgccctct tcgaagtgc 
      tcacagttg tgatgattc tcaacatgt taattgcga 
      cgatgtcgt cgacgtttc aacgagtcc tcgacaata 
      tcgacgtcg aatggacgt ttggaaaaa ccgattttt 
      atctagagt atatgctac aacccacac ttccaccac 
      cgttgaatt tgattccaa ctcctagcg gaatttcgg 
      cgtttatgg agtggtttc aatcgccaa aagttgatc 
      cgcgagaag aagaaaaga gaaatgatt atcctgcgc 
      tcatgtcgc agttggtgc aacgatatt ttagggaca 
      aagattcgg ccgcgagtc ctgaaaacg acttggagt 
      tcctcaagc aggaaatcg cagaaatta agcaagcgg 
      ttaatgacc tattagaga aggagtaat tttttttaa 
      taaaatatt ttaaagatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY