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Gene information
General information
TC013940 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2623819..2625153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013940
    iBeetle screen iB_09052

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013940 RA
      MPLFNKENM ITLIGENNK KNVLTDNKS NILVNSKAD 
      SDFPKIQDG CNMEFDPEL EPLLQENPR RFVVFPIQY 
      PDIWDKYKQ AEASFWTTE EVDLSKDLD DWKNKLNSD 
      ERYFISHVL AFFAASDGI VNENLVERF SQEVQVTEA 
      RCFYGFQIA IENIHSEMY SMLIETYIS EPQEKEFLF 
      NAIETLPCV KKKADWALL WINSKNATF GERIVAFAA 
      VEGIFFSGS FASIFWLKK RNLMPGLTF SNELISRDE 
      GLHCDFACL MFSHLVQKP SKERIQSII QDAVKIEKE 
      FLTEALPVS MIGMNCALM CQYIEFVAD RLLGELGCE 
      KIYNSQNPF DFMTIISNE GKTNFFEKK VGEYQKSGV 
      TCEQTEEYH LDLDF
    CDS sequence
    • >TC013940 RA
      atgccgctg tttaataaa gaaaacatg ataacctta 
      atcggtgaa aacaacaag aaaaacgtc ctcacggac 
      aacaaaagc aacattttg gtcaacagc aaagctgat 
      tcggacttc cccaagatc caggatggg tgcaacatg 
      gagttcgac cccgaattg gagcccctt ctgcaggaa 
      aacccgcgc cggtttgtg gtctttccc atccagtac 
      cccgacatt tgggacaaa tacaagcaa gcggaagcc 
      tcgttttgg acgaccgaa gaagtagat ttgtcgaaa 
      gatttggac gactggaag aacaaactg aattctgat 
      gaacgctac ttcatttcg cacgttctt gcatttttc 
      gcagcctct gacggaatc gtcaacgaa aacctcgtc 
      gagaggttc agccaggag gtgcaagtg accgaagct 
      cgctgcttt tacgggttc caaatcgct attgaaaac 
      atccattcg gagatgtac agcatgttg atcgaaacg 
      tacatttct gagccccag gagaaggag ttcttgttc 
      aatgcgatc gagacgctg ccctgtgtt aagaagaag 
      gccgactgg gccttgctg tggataaac agcaagaac 
      gcgactttt ggcgaacga atcgtggcg tttgccgcc 
      gtcgaaggg attttcttc tctggcagc tttgcttcg 
      attttctgg cttaaaaag cggaatctt atgccaggt 
      ctcacattt tccaatgaa ctgatttcg agggacgag 
      ggcctacat tgcgatttt gcctgtttg atgttctct 
      cacttggtg cagaagccg tccaaagaa cggattcaa 
      agcattatc caagacgcg gtcaaaatc gagaaggaa 
      ttcttgact gaagcttta ccagtcagc atgattgga 
      atgaactgc gctttgatg tgccaatac atcgaattt 
      gtggccgac agattgttg ggcgagttg gggtgcgag 
      aaaatctac aactcacag aatccgttc gatttcatg 
      acgattatt tccaacgaa ggcaagacg aatttcttt 
      gagaagaaa gtgggggaa taccagaag agcggagtc 
      acgtgtgag caaaccgag gagtaccat ctagatttg 
      gacttttaa
    mRNA sequence
    • >TC013940 RA
      cgctattca aatttaccc aattagagc ctttcccgc 
      ctcttctct tgcatatga tttcataac gaacctata 
      taagggttt gggcttttg gcgcctttt gttattgtc 
      gttccacta gtgttgggt gttgttcgt gttcctgtt 
      tacaaattg tgtgaatta aatcaccaa aatgccgct 
      gtttaataa agaaaacat gataacctt aatcggtga 
      aaacaacaa gaaaaacgt cctcacgga caacaaaag 
      caacatttt ggtcaacag caaagctga ttcggactt 
      ccccaagat ccaggatgg gtgcaacat ggagttcga 
      ccccgaatt ggagcccct tctgcagga aaacccgcg 
      ccggtttgt ggtctttcc catccagta ccccgacat 
      ttgggacaa atacaagca agcggaagc ctcgttttg 
      gacgaccga agaagtaga tttgtcgaa agatttgga 
      cgactggaa gaacaaact gaattctga tgaacgcta 
      cttcatttc gcacgttct tgcattttt cgcagcctc 
      tgacggaat cgtcaacga aaacctcgt cgagaggtt 
      cagccagga ggtgcaagt gaccgaagc tcgctgctt 
      ttacgggtt ccaaatcgc tattgaaaa catccattc 
      ggagatgta cagcatgtt gatcgaaac gtacatttc 
      tgagcccca ggagaagga gttcttgtt caatgcgat 
      cgagacgct gccctgtgt taagaagaa ggccgactg 
      ggccttgct gtggataaa cagcaagaa cgcgacttt 
      tggcgaacg aatcgtggc gtttgccgc cgtcgaagg 
      gattttctt ctctggcag ctttgcttc gattttctg 
      gcttaaaaa gcggaatct tatgccagg tctcacatt 
      ttccaatga actgatttc gagggacga gggcctaca 
      ttgcgattt tgcctgttt gatgttctc tcacttggt 
      gcagaagcc gtccaaaga acggattca aagcattat 
      ccaagacgc ggtcaaaat cgagaagga attcttgac 
      tgaagcttt accagtcag catgattgg aatgaactg 
      cgctttgat gtgccaata catcgaatt tgtggccga 
      cagattgtt gggcgagtt ggggtgcga gaaaatcta 
      caactcaca gaatccgtt cgatttcat gacgattat 
      ttccaacga aggcaagac gaatttctt tgagaagaa 
      agtggggga ataccagaa gagcggagt cacgtgtga 
      gcaaaccga ggagtacca tctagattt ggactttta 
      aagccaaaa gtgtttatt ttaaattat ttttattgt 
      agt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY