General information | |||
TC033022
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 2524020..2541277 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC033022 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09050 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 2 (13 dpi)
abdominal odoriferous glands mostly content decreased - Number of animals showing the phenotype: 4 (13 dpi)
mature egg not present - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033022 RA
MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG NDALGSLIK KLDQQYKIE TLLNLVNNK
- >TC033022 RB
MSFFNYSGF HSPPSRPLI IPSPTSPFC TSPSQLQEV TLDQDNTFS SEYFPSKEK RKIIILEAK TNLNAK
- >TC033022 RC
MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG NDALGSLIK KLDQQYKIE TLLNLCRCY FGFFGSFIS DNMQESGAD VTTDQSLTF ADITSPSQL QEVTLDQDN TFSSEYFPS KEKRKIIIL EAKTNLNAK
- >TC033022 RA
atgtattta ttttcgaaa acactatta cgaagggta ttactagtc cgaaaaaat tacgttttt tatacaacg tctaagtat gatctagat ttggccatc aaaggggtg tctctccga aattacatt aaacgattg gaacatgaa tatgagggg cttttaaaa ttgaacaat tacactgcc aatagtcgc tttcatcaa ttgcagcct ctaattaac gcactaaat gaaagaaaa gatgctgtc gaaaacata caaaatttg tccgaatta ttgaacgaa aaagacgat gatattagg aaactggca gaagaggaa aaactgacg tttgaaagc aggatcaag tctattgat gaaaaacta cttgaagca attttaccg tcagataag gaagatgag tgcaactcc atcgtattg gaagttaat gctggagtt gggggccaa gaggcaatg ttatttgct aacgaatta ttcgaaatg tattgtaat tttgccgag catcaaggc tgggaaacg caaatcgct gattactcc ccagttgac attggcgga atcaggcac gcaagtgcc ctaattaac ggacctcaa gtgtttcaa tttttcaag catgaagcg ggggttcac cgggtccag agaatcccg actacggaa aaggcgggt cgtattcac accagcact gtctcggtt gttgccctt ccacaaccc acagagatt gaagttaac atcgataac aaagatttg aaaattgag acgaaacgg gcgagtggg gctgggggg cagcacgtg aatacgacc gaaagtgcc gtccgggtg acacatttg cccacgggg tttagtgtg gagtgtcaa gtggacagg tctcaagtg aaaaatcgt caaattgct ttggctaag ttgagggct ttgatttat caacgtgat cttgaagag cagatagcg aggaacgag aatatgagg aagaaccaa gttaggtcg aattttaga aacgagaaa attaggact tataatttt ccgcaagac aggatcact gatcatagg ttacagggg tgcaatgtg cacaacctt aaagggttt cttcaaggg aatgatgct ttagggagt ttgattaag aaactagat caacagtac aaaattgag actttgtta aatcttgta aataataaa taa
- >TC033022 RB
atgtcgttc tttaattat agtggattt cacagcccg ccttcgagg cctttaatt attccgtcc cctacaagt cctttttgc acgagtcct agtcagctg caagaggtt actttggat caagacaac acattttca agtgagtat tttccttcc aaagagaaa cgcaaaatc atcattctc gaagcgaaa acgaatctg aatgccaag tga
- >TC033022 RC
atgtattta ttttcgaaa acactatta cgaagggta ttactagtc cgaaaaaat tacgttttt tatacaacg tctaagtat gatctagat ttggccatc aaaggggtg tctctccga aattacatt aaacgattg gaacatgaa tatgagggg cttttaaaa ttgaacaat tacactgcc aatagtcgc tttcatcaa ttgcagcct ctaattaac gcactaaat gaaagaaaa gatgctgtc gaaaacata caaaatttg tccgaatta ttgaacgaa aaagacgat gatattagg aaactggca gaagaggaa aaactgacg tttgaaagc aggatcaag tctattgat gaaaaacta cttgaagca attttaccg tcagataag gaagatgag tgcaactcc atcgtattg gaagttaat gctggagtt gggggccaa gaggcaatg ttatttgct aacgaatta ttcgaaatg tattgtaat tttgccgag catcaaggc tgggaaacg caaatcgct gattactcc ccagttgac attggcgga atcaggcac gcaagtgcc ctaattaac ggacctcaa gtgtttcaa tttttcaag catgaagcg ggggttcac cgggtccag agaatcccg actacggaa aaggcgggt cgtattcac accagcact gtctcggtt gttgccctt ccacaaccc acagagatt gaagttaac atcgataac aaagatttg aaaattgag acgaaacgg gcgagtggg gctgggggg cagcacgtg aatacgacc gaaagtgcc gtccgggtg acacatttg cccacgggg tttagtgtg gagtgtcaa gtggacagg tctcaagtg aaaaatcgt caaattgct ttggctaag ttgagggct ttgatttat caacgtgat cttgaagag cagatagcg aggaacgag aatatgagg aagaaccaa gttaggtcg aattttaga aacgagaaa attaggact tataatttt ccgcaagac aggatcact gatcatagg ttacagggg tgcaatgtg cacaacctt aaagggttt cttcaaggg aatgatgct ttagggagt ttgattaag aaactagat caacagtac aaaattgag actttgtta aatctttgt cgatgttat tttgggttt ttcggtagt tttataagc gacaatatg caagaaagt ggtgcagat gtcacaacg gatcagtcg ctgactttc gccgacatc acgagtcct agtcagctg caagaggtt actttggat caagacaac acattttca agtgagtat tttccttcc aaagagaaa cgcaaaatc atcattctc gaagcgaaa acgaatctg aatgccaag tga
- >TC033022 RA
ataaaaaca tgtatttat tttcgaaaa cactattac gaagggtat tactagtcc gaaaaaatt acgtttttt atacaacgt ctaagtatg atctagatt tggccatca aaggggtgt ctctccgaa attacatta aacgattgg aacatgaat atgaggggc ttttaaaat tgaacaatt acactgcca atagtcgct ttcatcaat tgcagcctc taattaacg cactaaatg aaagaaaag atgctgtcg aaaacatac aaaatttgt ccgaattat tgaacgaaa aagacgatg atattagga aactggcag aagaggaaa aactgacgt ttgaaagca ggatcaagt ctattgatg aaaaactac ttgaagcaa ttttaccgt cagataagg aagatgagt gcaactcca tcgtattgg aagttaatg ctggagttg ggggccaag aggcaatgt tatttgcta acgaattat tcgaaatgt attgtaatt ttgccgagc atcaaggct gggaaacgc aaatcgctg attactccc cagttgaca ttggcggaa tcaggcacg caagtgccc taattaacg gacctcaag tgtttcaat ttttcaagc atgaagcgg gggttcacc gggtccaga gaatcccga ctacggaaa aggcgggtc gtattcaca ccagcactg tctcggttg ttgcccttc cacaaccca cagagattg aagttaaca tcgataaca aagatttga aaattgaga cgaaacggg cgagtgggg ctggggggc agcacgtga atacgaccg aaagtgccg tccgggtga cacatttgc ccacggggt ttagtgtgg agtgtcaag tggacaggt ctcaagtga aaaatcgtc aaattgctt tggctaagt tgagggctt tgatttatc aacgtgatc ttgaagagc agatagcga ggaacgaga atatgagga agaaccaag ttaggtcga attttagaa acgagaaaa ttaggactt ataattttc cgcaagaca ggatcactg atcataggt tacaggggt gcaatgtgc acaacctta aagggtttc ttcaaggga atgatgctt tagggagtt tgattaaga aactagatc aacagtaca aaattgaga ctttgttaa atcttgtaa ataataaat aaatgagtt caaattggc gcctagttt aattttgta gtgatttcc tctttgtta acattggta gcattgcag tgtcacggc aacaaccaa tcataacaa accaaattg aaataaaaa tatttaaac gtcagtttt aacggtttt attcttgtg gctgtcagt gtcgatgtt attttgggt ttttcggta gttttataa gcgacaata tgcaagaaa gtggtgcag atgtcacaa cggatcagt cgctgactt tcgccgaca tgtaagaca
- >TC033022 RB
ccaaacatt tcagtggag aagtgtaga gaggataga gtggtggta aagtgcgcc ggtttgttg tgactacga aaagtttat tattaattt taatttcac gttttaaag tgagacaag aagggcaaa gaaaaaatg cccccttaa aaataactt cggtttatg gacgctcgt aatagtggc ataaatttt caagtgctt gaaatcatg tcgttcttt aattatagt ggatttcac agcccgcct tcgaggcct ttaattatt ccgtcccct acaagtcct ttttgcacg agtcctagt cagctgcaa gaggttact ttggatcaa gacaacaca ttttcaagt gagtatttt ccttccaaa gagaaacgc aaaatcatc attctcgaa gcgaaaacg aatctgaat gccaagtga taacttggc acaaatatt tcgttcaaa cgccaaaaa aaacctttg tgttatgtt tggtaaagc gtacagcac tgattaaag taaagcatt attcttcat cacgtaata aaccaattc ggtggatta agttgccgc cttgtctca tttccggca gtttgaaat gagtatgtt caaatctac cccacctag gaattattt aataatata ctcggttgc ggttggttg gaggttatg gaacgacaa cgggtgctg ctaattgcc ctgaagacg atgttttta ttaattagg cgctttgtg cgccaccgt tttggcaga cttccatgc tggaatgac aaacaacaa ttttggaac gttcagaaa tagcgattt caagggcga aacttcgat aagtacgct tcagtcttt cagcgggtt atgtaagag gctgtttca atgcagagt aataaattt aatgttcgc at
- >TC033022 RC
ataaaaaca tgtatttat tttcgaaaa cactattac gaagggtat tactagtcc gaaaaaatt acgtttttt atacaacgt ctaagtatg atctagatt tggccatca aaggggtgt ctctccgaa attacatta aacgattgg aacatgaat atgaggggc ttttaaaat tgaacaatt acactgcca atagtcgct ttcatcaat tgcagcctc taattaacg cactaaatg aaagaaaag atgctgtcg aaaacatac aaaatttgt ccgaattat tgaacgaaa aagacgatg atattagga aactggcag aagaggaaa aactgacgt ttgaaagca ggatcaagt ctattgatg aaaaactac ttgaagcaa ttttaccgt cagataagg aagatgagt gcaactcca tcgtattgg aagttaatg ctggagttg ggggccaag aggcaatgt tatttgcta acgaattat tcgaaatgt attgtaatt ttgccgagc atcaaggct gggaaacgc aaatcgctg attactccc cagttgaca ttggcggaa tcaggcacg caagtgccc taattaacg gacctcaag tgtttcaat ttttcaagc atgaagcgg gggttcacc gggtccaga gaatcccga ctacggaaa aggcgggtc gtattcaca ccagcactg tctcggttg ttgcccttc cacaaccca cagagattg aagttaaca tcgataaca aagatttga aaattgaga cgaaacggg cgagtgggg ctggggggc agcacgtga atacgaccg aaagtgccg tccgggtga cacatttgc ccacggggt ttagtgtgg agtgtcaag tggacaggt ctcaagtga aaaatcgtc aaattgctt tggctaagt tgagggctt tgatttatc aacgtgatc ttgaagagc agatagcga ggaacgaga atatgagga agaaccaag ttaggtcga attttagaa acgagaaaa ttaggactt ataattttc cgcaagaca ggatcactg atcataggt tacaggggt gcaatgtgc acaacctta aagggtttc ttcaaggga atgatgctt tagggagtt tgattaaga aactagatc aacagtaca aaattgaga ctttgttaa atctttgtc gatgttatt ttgggtttt tcggtagtt ttataagcg acaatatgc aagaaagtg gtgcagatg tcacaacgg atcagtcgc tgactttcg ccgacatca cgagtccta gtcagctgc aagaggtta ctttggatc aagacaaca cattttcaa gtgagtatt ttccttcca aagagaaac gcaaaatca tcattctcg aagcgaaaa cgaatctga atgccaagt gataacttg gcacaaata tttcgttca aacgccaaa aaaaacctt tgtgttatg tttggtaaa gcgtacagc actgattaa agtaaagca ttattcttc atcacgtaa taaaccaat tcggtggat taagttgcc gccttgtct catttccgg cagtttgaa atgagtatg ttcaaatct accccacct aggaattat ttaataata tactcggtt gcggttggt tggaggtta tggaacgac aacgggtgc tgctaattg ccctgaaga cgatgtttt tattaatta ggcgctttg tgcgccacc gttttggca gacttccat gctggaatg acaaacaac aattttgga acgttcaga aatagcgat ttcaagggc gaaacttcg ataagtacg cttcagtct ttcagcggg ttatgtaag aggctgttt caatgcaga gtaataaat ttaatgttc gcat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
