Status
Please wait ...
Gene information
General information
TC033022
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013879,TC013880
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2524020..2541277
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033022
    iBeetle screen iB_09050

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands mostly content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033022 RA
      MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV 
      SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN 
      ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT 
      FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV 
      GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD 
      IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE 
      KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR 
      ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV 
      KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS 
      NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG 
      NDALGSLIK KLDQQYKIE TLLNLVNNK
    • >TC033022 RB
      MSFFNYSGF HSPPSRPLI IPSPTSPFC TSPSQLQEV 
      TLDQDNTFS SEYFPSKEK RKIIILEAK TNLNAK
    • >TC033022 RC
      MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV 
      SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN 
      ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT 
      FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV 
      GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD 
      IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE 
      KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR 
      ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV 
      KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS 
      NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG 
      NDALGSLIK KLDQQYKIE TLLNLCRCY FGFFGSFIS 
      DNMQESGAD VTTDQSLTF ADITSPSQL QEVTLDQDN 
      TFSSEYFPS KEKRKIIIL EAKTNLNAK
    CDS sequence
    • >TC033022 RA
      atgtattta ttttcgaaa acactatta cgaagggta 
      ttactagtc cgaaaaaat tacgttttt tatacaacg 
      tctaagtat gatctagat ttggccatc aaaggggtg 
      tctctccga aattacatt aaacgattg gaacatgaa 
      tatgagggg cttttaaaa ttgaacaat tacactgcc 
      aatagtcgc tttcatcaa ttgcagcct ctaattaac 
      gcactaaat gaaagaaaa gatgctgtc gaaaacata 
      caaaatttg tccgaatta ttgaacgaa aaagacgat 
      gatattagg aaactggca gaagaggaa aaactgacg 
      tttgaaagc aggatcaag tctattgat gaaaaacta 
      cttgaagca attttaccg tcagataag gaagatgag 
      tgcaactcc atcgtattg gaagttaat gctggagtt 
      gggggccaa gaggcaatg ttatttgct aacgaatta 
      ttcgaaatg tattgtaat tttgccgag catcaaggc 
      tgggaaacg caaatcgct gattactcc ccagttgac 
      attggcgga atcaggcac gcaagtgcc ctaattaac 
      ggacctcaa gtgtttcaa tttttcaag catgaagcg 
      ggggttcac cgggtccag agaatcccg actacggaa 
      aaggcgggt cgtattcac accagcact gtctcggtt 
      gttgccctt ccacaaccc acagagatt gaagttaac 
      atcgataac aaagatttg aaaattgag acgaaacgg 
      gcgagtggg gctgggggg cagcacgtg aatacgacc 
      gaaagtgcc gtccgggtg acacatttg cccacgggg 
      tttagtgtg gagtgtcaa gtggacagg tctcaagtg 
      aaaaatcgt caaattgct ttggctaag ttgagggct 
      ttgatttat caacgtgat cttgaagag cagatagcg 
      aggaacgag aatatgagg aagaaccaa gttaggtcg 
      aattttaga aacgagaaa attaggact tataatttt 
      ccgcaagac aggatcact gatcatagg ttacagggg 
      tgcaatgtg cacaacctt aaagggttt cttcaaggg 
      aatgatgct ttagggagt ttgattaag aaactagat 
      caacagtac aaaattgag actttgtta aatcttgta 
      aataataaa taa
    • >TC033022 RB
      atgtcgttc tttaattat agtggattt cacagcccg 
      ccttcgagg cctttaatt attccgtcc cctacaagt 
      cctttttgc acgagtcct agtcagctg caagaggtt 
      actttggat caagacaac acattttca agtgagtat 
      tttccttcc aaagagaaa cgcaaaatc atcattctc 
      gaagcgaaa acgaatctg aatgccaag tga
    • >TC033022 RC
      atgtattta ttttcgaaa acactatta cgaagggta 
      ttactagtc cgaaaaaat tacgttttt tatacaacg 
      tctaagtat gatctagat ttggccatc aaaggggtg 
      tctctccga aattacatt aaacgattg gaacatgaa 
      tatgagggg cttttaaaa ttgaacaat tacactgcc 
      aatagtcgc tttcatcaa ttgcagcct ctaattaac 
      gcactaaat gaaagaaaa gatgctgtc gaaaacata 
      caaaatttg tccgaatta ttgaacgaa aaagacgat 
      gatattagg aaactggca gaagaggaa aaactgacg 
      tttgaaagc aggatcaag tctattgat gaaaaacta 
      cttgaagca attttaccg tcagataag gaagatgag 
      tgcaactcc atcgtattg gaagttaat gctggagtt 
      gggggccaa gaggcaatg ttatttgct aacgaatta 
      ttcgaaatg tattgtaat tttgccgag catcaaggc 
      tgggaaacg caaatcgct gattactcc ccagttgac 
      attggcgga atcaggcac gcaagtgcc ctaattaac 
      ggacctcaa gtgtttcaa tttttcaag catgaagcg 
      ggggttcac cgggtccag agaatcccg actacggaa 
      aaggcgggt cgtattcac accagcact gtctcggtt 
      gttgccctt ccacaaccc acagagatt gaagttaac 
      atcgataac aaagatttg aaaattgag acgaaacgg 
      gcgagtggg gctgggggg cagcacgtg aatacgacc 
      gaaagtgcc gtccgggtg acacatttg cccacgggg 
      tttagtgtg gagtgtcaa gtggacagg tctcaagtg 
      aaaaatcgt caaattgct ttggctaag ttgagggct 
      ttgatttat caacgtgat cttgaagag cagatagcg 
      aggaacgag aatatgagg aagaaccaa gttaggtcg 
      aattttaga aacgagaaa attaggact tataatttt 
      ccgcaagac aggatcact gatcatagg ttacagggg 
      tgcaatgtg cacaacctt aaagggttt cttcaaggg 
      aatgatgct ttagggagt ttgattaag aaactagat 
      caacagtac aaaattgag actttgtta aatctttgt 
      cgatgttat tttgggttt ttcggtagt tttataagc 
      gacaatatg caagaaagt ggtgcagat gtcacaacg 
      gatcagtcg ctgactttc gccgacatc acgagtcct 
      agtcagctg caagaggtt actttggat caagacaac 
      acattttca agtgagtat tttccttcc aaagagaaa 
      cgcaaaatc atcattctc gaagcgaaa acgaatctg 
      aatgccaag tga
    mRNA sequence
    • >TC033022 RA
      ataaaaaca tgtatttat tttcgaaaa cactattac 
      gaagggtat tactagtcc gaaaaaatt acgtttttt 
      atacaacgt ctaagtatg atctagatt tggccatca 
      aaggggtgt ctctccgaa attacatta aacgattgg 
      aacatgaat atgaggggc ttttaaaat tgaacaatt 
      acactgcca atagtcgct ttcatcaat tgcagcctc 
      taattaacg cactaaatg aaagaaaag atgctgtcg 
      aaaacatac aaaatttgt ccgaattat tgaacgaaa 
      aagacgatg atattagga aactggcag aagaggaaa 
      aactgacgt ttgaaagca ggatcaagt ctattgatg 
      aaaaactac ttgaagcaa ttttaccgt cagataagg 
      aagatgagt gcaactcca tcgtattgg aagttaatg 
      ctggagttg ggggccaag aggcaatgt tatttgcta 
      acgaattat tcgaaatgt attgtaatt ttgccgagc 
      atcaaggct gggaaacgc aaatcgctg attactccc 
      cagttgaca ttggcggaa tcaggcacg caagtgccc 
      taattaacg gacctcaag tgtttcaat ttttcaagc 
      atgaagcgg gggttcacc gggtccaga gaatcccga 
      ctacggaaa aggcgggtc gtattcaca ccagcactg 
      tctcggttg ttgcccttc cacaaccca cagagattg 
      aagttaaca tcgataaca aagatttga aaattgaga 
      cgaaacggg cgagtgggg ctggggggc agcacgtga 
      atacgaccg aaagtgccg tccgggtga cacatttgc 
      ccacggggt ttagtgtgg agtgtcaag tggacaggt 
      ctcaagtga aaaatcgtc aaattgctt tggctaagt 
      tgagggctt tgatttatc aacgtgatc ttgaagagc 
      agatagcga ggaacgaga atatgagga agaaccaag 
      ttaggtcga attttagaa acgagaaaa ttaggactt 
      ataattttc cgcaagaca ggatcactg atcataggt 
      tacaggggt gcaatgtgc acaacctta aagggtttc 
      ttcaaggga atgatgctt tagggagtt tgattaaga 
      aactagatc aacagtaca aaattgaga ctttgttaa 
      atcttgtaa ataataaat aaatgagtt caaattggc 
      gcctagttt aattttgta gtgatttcc tctttgtta 
      acattggta gcattgcag tgtcacggc aacaaccaa 
      tcataacaa accaaattg aaataaaaa tatttaaac 
      gtcagtttt aacggtttt attcttgtg gctgtcagt 
      gtcgatgtt attttgggt ttttcggta gttttataa 
      gcgacaata tgcaagaaa gtggtgcag atgtcacaa 
      cggatcagt cgctgactt tcgccgaca tgtaagaca
    • >TC033022 RB
      ccaaacatt tcagtggag aagtgtaga gaggataga 
      gtggtggta aagtgcgcc ggtttgttg tgactacga 
      aaagtttat tattaattt taatttcac gttttaaag 
      tgagacaag aagggcaaa gaaaaaatg cccccttaa 
      aaataactt cggtttatg gacgctcgt aatagtggc 
      ataaatttt caagtgctt gaaatcatg tcgttcttt 
      aattatagt ggatttcac agcccgcct tcgaggcct 
      ttaattatt ccgtcccct acaagtcct ttttgcacg 
      agtcctagt cagctgcaa gaggttact ttggatcaa 
      gacaacaca ttttcaagt gagtatttt ccttccaaa 
      gagaaacgc aaaatcatc attctcgaa gcgaaaacg 
      aatctgaat gccaagtga taacttggc acaaatatt 
      tcgttcaaa cgccaaaaa aaacctttg tgttatgtt 
      tggtaaagc gtacagcac tgattaaag taaagcatt 
      attcttcat cacgtaata aaccaattc ggtggatta 
      agttgccgc cttgtctca tttccggca gtttgaaat 
      gagtatgtt caaatctac cccacctag gaattattt 
      aataatata ctcggttgc ggttggttg gaggttatg 
      gaacgacaa cgggtgctg ctaattgcc ctgaagacg 
      atgttttta ttaattagg cgctttgtg cgccaccgt 
      tttggcaga cttccatgc tggaatgac aaacaacaa 
      ttttggaac gttcagaaa tagcgattt caagggcga 
      aacttcgat aagtacgct tcagtcttt cagcgggtt 
      atgtaagag gctgtttca atgcagagt aataaattt 
      aatgttcgc at
    • >TC033022 RC
      ataaaaaca tgtatttat tttcgaaaa cactattac 
      gaagggtat tactagtcc gaaaaaatt acgtttttt 
      atacaacgt ctaagtatg atctagatt tggccatca 
      aaggggtgt ctctccgaa attacatta aacgattgg 
      aacatgaat atgaggggc ttttaaaat tgaacaatt 
      acactgcca atagtcgct ttcatcaat tgcagcctc 
      taattaacg cactaaatg aaagaaaag atgctgtcg 
      aaaacatac aaaatttgt ccgaattat tgaacgaaa 
      aagacgatg atattagga aactggcag aagaggaaa 
      aactgacgt ttgaaagca ggatcaagt ctattgatg 
      aaaaactac ttgaagcaa ttttaccgt cagataagg 
      aagatgagt gcaactcca tcgtattgg aagttaatg 
      ctggagttg ggggccaag aggcaatgt tatttgcta 
      acgaattat tcgaaatgt attgtaatt ttgccgagc 
      atcaaggct gggaaacgc aaatcgctg attactccc 
      cagttgaca ttggcggaa tcaggcacg caagtgccc 
      taattaacg gacctcaag tgtttcaat ttttcaagc 
      atgaagcgg gggttcacc gggtccaga gaatcccga 
      ctacggaaa aggcgggtc gtattcaca ccagcactg 
      tctcggttg ttgcccttc cacaaccca cagagattg 
      aagttaaca tcgataaca aagatttga aaattgaga 
      cgaaacggg cgagtgggg ctggggggc agcacgtga 
      atacgaccg aaagtgccg tccgggtga cacatttgc 
      ccacggggt ttagtgtgg agtgtcaag tggacaggt 
      ctcaagtga aaaatcgtc aaattgctt tggctaagt 
      tgagggctt tgatttatc aacgtgatc ttgaagagc 
      agatagcga ggaacgaga atatgagga agaaccaag 
      ttaggtcga attttagaa acgagaaaa ttaggactt 
      ataattttc cgcaagaca ggatcactg atcataggt 
      tacaggggt gcaatgtgc acaacctta aagggtttc 
      ttcaaggga atgatgctt tagggagtt tgattaaga 
      aactagatc aacagtaca aaattgaga ctttgttaa 
      atctttgtc gatgttatt ttgggtttt tcggtagtt 
      ttataagcg acaatatgc aagaaagtg gtgcagatg 
      tcacaacgg atcagtcgc tgactttcg ccgacatca 
      cgagtccta gtcagctgc aagaggtta ctttggatc 
      aagacaaca cattttcaa gtgagtatt ttccttcca 
      aagagaaac gcaaaatca tcattctcg aagcgaaaa 
      cgaatctga atgccaagt gataacttg gcacaaata 
      tttcgttca aacgccaaa aaaaacctt tgtgttatg 
      tttggtaaa gcgtacagc actgattaa agtaaagca 
      ttattcttc atcacgtaa taaaccaat tcggtggat 
      taagttgcc gccttgtct catttccgg cagtttgaa 
      atgagtatg ttcaaatct accccacct aggaattat 
      ttaataata tactcggtt gcggttggt tggaggtta 
      tggaacgac aacgggtgc tgctaattg ccctgaaga 
      cgatgtttt tattaatta ggcgctttg tgcgccacc 
      gttttggca gacttccat gctggaatg acaaacaac 
      aattttgga acgttcaga aatagcgat ttcaagggc 
      gaaacttcg ataagtacg cttcagtct ttcagcggg 
      ttatgtaag aggctgttt caatgcaga gtaataaat 
      ttaatgttc gcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY