Status
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Gene information
General information
TC033022
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013879,TC013880 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2524020..2541277
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033022
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09050

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    abdominal odoriferous glands mostly content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    mature egg not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033022 RA
      MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV 
SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN 
ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT 
FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV 
GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD 
IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE 
KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR 
ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV 
KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS 
NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG 
NDALGSLIK KLDQQYKIE TLLNLVNNK
    • >TC033022 RB
      MSFFNYSGF HSPPSRPLI IPSPTSPFC TSPSQLQEV 
TLDQDNTFS SEYFPSKEK RKIIILEAK TNLNAK
    • >TC033022 RC
      MYLFSKTLL RRVLLVRKN YVFYTTSKY DLDLAIKGV 
SLRNYIKRL EHEYEGLLK LNNYTANSR FHQLQPLIN 
ALNERKDAV ENIQNLSEL LNEKDDDIR KLAEEEKLT 
FESRIKSID EKLLEAILP SDKEDECNS IVLEVNAGV 
GGQEAMLFA NELFEMYCN FAEHQGWET QIADYSPVD 
IGGIRHASA LINGPQVFQ FFKHEAGVH RVQRIPTTE 
KAGRIHTST VSVVALPQP TEIEVNIDN KDLKIETKR 
ASGAGGQHV NTTESAVRV THLPTGFSV ECQVDRSQV 
KNRQIALAK LRALIYQRD LEEQIARNE NMRKNQVRS 
NFRNEKIRT YNFPQDRIT DHRLQGCNV HNLKGFLQG 
NDALGSLIK KLDQQYKIE TLLNLCRCY FGFFGSFIS 
DNMQESGAD VTTDQSLTF ADITSPSQL QEVTLDQDN 
TFSSEYFPS KEKRKIIIL EAKTNLNAK
    CDS sequence
    • >TC033022 RA
      atgtattta ttttcgaaa acactatta cgaagggta 
ttactagtc cgaaaaaat tacgttttt tatacaacg 
tctaagtat gatctagat ttggccatc aaaggggtg 
tctctccga aattacatt aaacgattg gaacatgaa 
tatgagggg cttttaaaa ttgaacaat tacactgcc 
aatagtcgc tttcatcaa ttgcagcct ctaattaac 
gcactaaat gaaagaaaa gatgctgtc gaaaacata 
caaaatttg tccgaatta ttgaacgaa aaagacgat 
gatattagg aaactggca gaagaggaa aaactgacg 
tttgaaagc aggatcaag tctattgat gaaaaacta 
cttgaagca attttaccg tcagataag gaagatgag 
tgcaactcc atcgtattg gaagttaat gctggagtt 
gggggccaa gaggcaatg ttatttgct aacgaatta 
ttcgaaatg tattgtaat tttgccgag catcaaggc 
tgggaaacg caaatcgct gattactcc ccagttgac 
attggcgga atcaggcac gcaagtgcc ctaattaac 
ggacctcaa gtgtttcaa tttttcaag catgaagcg 
ggggttcac cgggtccag agaatcccg actacggaa 
aaggcgggt cgtattcac accagcact gtctcggtt 
gttgccctt ccacaaccc acagagatt gaagttaac 
atcgataac aaagatttg aaaattgag acgaaacgg 
gcgagtggg gctgggggg cagcacgtg aatacgacc 
gaaagtgcc gtccgggtg acacatttg cccacgggg 
tttagtgtg gagtgtcaa gtggacagg tctcaagtg 
aaaaatcgt caaattgct ttggctaag ttgagggct 
ttgatttat caacgtgat cttgaagag cagatagcg 
aggaacgag aatatgagg aagaaccaa gttaggtcg 
aattttaga aacgagaaa attaggact tataatttt 
ccgcaagac aggatcact gatcatagg ttacagggg 
tgcaatgtg cacaacctt aaagggttt cttcaaggg 
aatgatgct ttagggagt ttgattaag aaactagat 
caacagtac aaaattgag actttgtta aatcttgta 
aataataaa taa
    • >TC033022 RB
      atgtcgttc tttaattat agtggattt cacagcccg 
ccttcgagg cctttaatt attccgtcc cctacaagt 
cctttttgc acgagtcct agtcagctg caagaggtt 
actttggat caagacaac acattttca agtgagtat 
tttccttcc aaagagaaa cgcaaaatc atcattctc 
gaagcgaaa acgaatctg aatgccaag tga
    • >TC033022 RC
      atgtattta ttttcgaaa acactatta cgaagggta 
ttactagtc cgaaaaaat tacgttttt tatacaacg 
tctaagtat gatctagat ttggccatc aaaggggtg 
tctctccga aattacatt aaacgattg gaacatgaa 
tatgagggg cttttaaaa ttgaacaat tacactgcc 
aatagtcgc tttcatcaa ttgcagcct ctaattaac 
gcactaaat gaaagaaaa gatgctgtc gaaaacata 
caaaatttg tccgaatta ttgaacgaa aaagacgat 
gatattagg aaactggca gaagaggaa aaactgacg 
tttgaaagc aggatcaag tctattgat gaaaaacta 
cttgaagca attttaccg tcagataag gaagatgag 
tgcaactcc atcgtattg gaagttaat gctggagtt 
gggggccaa gaggcaatg ttatttgct aacgaatta 
ttcgaaatg tattgtaat tttgccgag catcaaggc 
tgggaaacg caaatcgct gattactcc ccagttgac 
attggcgga atcaggcac gcaagtgcc ctaattaac 
ggacctcaa gtgtttcaa tttttcaag catgaagcg 
ggggttcac cgggtccag agaatcccg actacggaa 
aaggcgggt cgtattcac accagcact gtctcggtt 
gttgccctt ccacaaccc acagagatt gaagttaac 
atcgataac aaagatttg aaaattgag acgaaacgg 
gcgagtggg gctgggggg cagcacgtg aatacgacc 
gaaagtgcc gtccgggtg acacatttg cccacgggg 
tttagtgtg gagtgtcaa gtggacagg tctcaagtg 
aaaaatcgt caaattgct ttggctaag ttgagggct 
ttgatttat caacgtgat cttgaagag cagatagcg 
aggaacgag aatatgagg aagaaccaa gttaggtcg 
aattttaga aacgagaaa attaggact tataatttt 
ccgcaagac aggatcact gatcatagg ttacagggg 
tgcaatgtg cacaacctt aaagggttt cttcaaggg 
aatgatgct ttagggagt ttgattaag aaactagat 
caacagtac aaaattgag actttgtta aatctttgt 
cgatgttat tttgggttt ttcggtagt tttataagc 
gacaatatg caagaaagt ggtgcagat gtcacaacg 
gatcagtcg ctgactttc gccgacatc acgagtcct 
agtcagctg caagaggtt actttggat caagacaac 
acattttca agtgagtat tttccttcc aaagagaaa 
cgcaaaatc atcattctc gaagcgaaa acgaatctg 
aatgccaag tga
    mRNA sequence
    • >TC033022 RA
      ataaaaaca tgtatttat tttcgaaaa cactattac 
gaagggtat tactagtcc gaaaaaatt acgtttttt 
atacaacgt ctaagtatg atctagatt tggccatca 
aaggggtgt ctctccgaa attacatta aacgattgg 
aacatgaat atgaggggc ttttaaaat tgaacaatt 
acactgcca atagtcgct ttcatcaat tgcagcctc 
taattaacg cactaaatg aaagaaaag atgctgtcg 
aaaacatac aaaatttgt ccgaattat tgaacgaaa 
aagacgatg atattagga aactggcag aagaggaaa 
aactgacgt ttgaaagca ggatcaagt ctattgatg 
aaaaactac ttgaagcaa ttttaccgt cagataagg 
aagatgagt gcaactcca tcgtattgg aagttaatg 
ctggagttg ggggccaag aggcaatgt tatttgcta 
acgaattat tcgaaatgt attgtaatt ttgccgagc 
atcaaggct gggaaacgc aaatcgctg attactccc 
cagttgaca ttggcggaa tcaggcacg caagtgccc 
taattaacg gacctcaag tgtttcaat ttttcaagc 
atgaagcgg gggttcacc gggtccaga gaatcccga 
ctacggaaa aggcgggtc gtattcaca ccagcactg 
tctcggttg ttgcccttc cacaaccca cagagattg 
aagttaaca tcgataaca aagatttga aaattgaga 
cgaaacggg cgagtgggg ctggggggc agcacgtga 
atacgaccg aaagtgccg tccgggtga cacatttgc 
ccacggggt ttagtgtgg agtgtcaag tggacaggt 
ctcaagtga aaaatcgtc aaattgctt tggctaagt 
tgagggctt tgatttatc aacgtgatc ttgaagagc 
agatagcga ggaacgaga atatgagga agaaccaag 
ttaggtcga attttagaa acgagaaaa ttaggactt 
ataattttc cgcaagaca ggatcactg atcataggt 
tacaggggt gcaatgtgc acaacctta aagggtttc 
ttcaaggga atgatgctt tagggagtt tgattaaga 
aactagatc aacagtaca aaattgaga ctttgttaa 
atcttgtaa ataataaat aaatgagtt caaattggc 
gcctagttt aattttgta gtgatttcc tctttgtta 
acattggta gcattgcag tgtcacggc aacaaccaa 
tcataacaa accaaattg aaataaaaa tatttaaac 
gtcagtttt aacggtttt attcttgtg gctgtcagt 
gtcgatgtt attttgggt ttttcggta gttttataa 
gcgacaata tgcaagaaa gtggtgcag atgtcacaa 
cggatcagt cgctgactt tcgccgaca tgtaagaca
    • >TC033022 RB
      ccaaacatt tcagtggag aagtgtaga gaggataga 
gtggtggta aagtgcgcc ggtttgttg tgactacga 
aaagtttat tattaattt taatttcac gttttaaag 
tgagacaag aagggcaaa gaaaaaatg cccccttaa 
aaataactt cggtttatg gacgctcgt aatagtggc 
ataaatttt caagtgctt gaaatcatg tcgttcttt 
aattatagt ggatttcac agcccgcct tcgaggcct 
ttaattatt ccgtcccct acaagtcct ttttgcacg 
agtcctagt cagctgcaa gaggttact ttggatcaa 
gacaacaca ttttcaagt gagtatttt ccttccaaa 
gagaaacgc aaaatcatc attctcgaa gcgaaaacg 
aatctgaat gccaagtga taacttggc acaaatatt 
tcgttcaaa cgccaaaaa aaacctttg tgttatgtt 
tggtaaagc gtacagcac tgattaaag taaagcatt 
attcttcat cacgtaata aaccaattc ggtggatta 
agttgccgc cttgtctca tttccggca gtttgaaat 
gagtatgtt caaatctac cccacctag gaattattt 
aataatata ctcggttgc ggttggttg gaggttatg 
gaacgacaa cgggtgctg ctaattgcc ctgaagacg 
atgttttta ttaattagg cgctttgtg cgccaccgt 
tttggcaga cttccatgc tggaatgac aaacaacaa 
ttttggaac gttcagaaa tagcgattt caagggcga 
aacttcgat aagtacgct tcagtcttt cagcgggtt 
atgtaagag gctgtttca atgcagagt aataaattt 
aatgttcgc at
    • >TC033022 RC
      ataaaaaca tgtatttat tttcgaaaa cactattac 
gaagggtat tactagtcc gaaaaaatt acgtttttt 
atacaacgt ctaagtatg atctagatt tggccatca 
aaggggtgt ctctccgaa attacatta aacgattgg 
aacatgaat atgaggggc ttttaaaat tgaacaatt 
acactgcca atagtcgct ttcatcaat tgcagcctc 
taattaacg cactaaatg aaagaaaag atgctgtcg 
aaaacatac aaaatttgt ccgaattat tgaacgaaa 
aagacgatg atattagga aactggcag aagaggaaa 
aactgacgt ttgaaagca ggatcaagt ctattgatg 
aaaaactac ttgaagcaa ttttaccgt cagataagg 
aagatgagt gcaactcca tcgtattgg aagttaatg 
ctggagttg ggggccaag aggcaatgt tatttgcta 
acgaattat tcgaaatgt attgtaatt ttgccgagc 
atcaaggct gggaaacgc aaatcgctg attactccc 
cagttgaca ttggcggaa tcaggcacg caagtgccc 
taattaacg gacctcaag tgtttcaat ttttcaagc 
atgaagcgg gggttcacc gggtccaga gaatcccga 
ctacggaaa aggcgggtc gtattcaca ccagcactg 
tctcggttg ttgcccttc cacaaccca cagagattg 
aagttaaca tcgataaca aagatttga aaattgaga 
cgaaacggg cgagtgggg ctggggggc agcacgtga 
atacgaccg aaagtgccg tccgggtga cacatttgc 
ccacggggt ttagtgtgg agtgtcaag tggacaggt 
ctcaagtga aaaatcgtc aaattgctt tggctaagt 
tgagggctt tgatttatc aacgtgatc ttgaagagc 
agatagcga ggaacgaga atatgagga agaaccaag 
ttaggtcga attttagaa acgagaaaa ttaggactt 
ataattttc cgcaagaca ggatcactg atcataggt 
tacaggggt gcaatgtgc acaacctta aagggtttc 
ttcaaggga atgatgctt tagggagtt tgattaaga 
aactagatc aacagtaca aaattgaga ctttgttaa 
atctttgtc gatgttatt ttgggtttt tcggtagtt 
ttataagcg acaatatgc aagaaagtg gtgcagatg 
tcacaacgg atcagtcgc tgactttcg ccgacatca 
cgagtccta gtcagctgc aagaggtta ctttggatc 
aagacaaca cattttcaa gtgagtatt ttccttcca 
aagagaaac gcaaaatca tcattctcg aagcgaaaa 
cgaatctga atgccaagt gataacttg gcacaaata 
tttcgttca aacgccaaa aaaaacctt tgtgttatg 
tttggtaaa gcgtacagc actgattaa agtaaagca 
ttattcttc atcacgtaa taaaccaat tcggtggat 
taagttgcc gccttgtct catttccgg cagtttgaa 
atgagtatg ttcaaatct accccacct aggaattat 
ttaataata tactcggtt gcggttggt tggaggtta 
tggaacgac aacgggtgc tgctaattg ccctgaaga 
cgatgtttt tattaatta ggcgctttg tgcgccacc 
gttttggca gacttccat gctggaatg acaaacaac 
aattttgga acgttcaga aatagcgat ttcaagggc 
gaaacttcg ataagtacg cttcagtct ttcagcggg 
ttatgtaag aggctgttt caatgcaga gtaataaat 
ttaatgttc gcat

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY