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Gene information
General information
TC013920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2158817..2159908
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013920
    iBeetle screen iB_09048

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 1

    ovary without detectable defects - Number of animals showing the phenotype: 2
    previtellogenic egg chamber complete not present - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013920 RA
      MANVIARRF FNRNLFKFN ELAQLATHS RCTATVIIN 
      FNHNYGTPN PRNHKLLQP FKTTEIQHF YSDLSKNKK 
      KPSIFQRFK QMYKEYWYV LLPVHLVTS AAWFGGFYY 
      LAKSGVDIV GILESWNIS ERIVNPLRD SSMGYVAVA 
      YALYKIATP ARYTVTLGG TTISINYLK KWGYIKPVP 
      SKERLKEMY FEQKDNLLE TVRETKEGL KDKKENIIE 
      DLDKGIKQL KEIGDKMKS KDTVVDDVK KKAKGSKDK 
      G
    CDS sequence
    • >TC013920 RA
      atggcaaat gtaattgca agaaggttt ttcaaccgg 
      aatttattt aaatttaat gaactggct caactggca 
      acacacagc agatgcacg gctactgtc atcataaat 
      tttaatcat aattatggt actcccaac ccaaggaat 
      cacaagttg ctccaaccg ttcaaaact acggaaatt 
      cagcacttt tattcagac ttgtccaaa aacaagaaa 
      aaaccttcg atatttcag aggtttaag cagatgtat 
      aaggagtac tggtatgtc cttctcccg gttcatctt 
      gtcacttct gcagcatgg tttggtggt ttttattat 
      ttagccaaa agtggtgtg gatattgtg ggaattttg 
      gaatcatgg aacattagt gagaggatt gtgaatccc 
      ttgagagac tcaagtatg ggctatgtc gctgttgct 
      tacgctctt tataaaata gcgacgcct gcgaggtac 
      acagtcacg ttaggtgga accacgatt tcaatcaat 
      tatctgaaa aagtggggc tacatcaaa ccagttcct 
      agtaaagag aggctcaaa gaaatgtac tttgaacag 
      aaagataac ttgttggag acagtaaga gagaccaag 
      gaaggtttg aaagacaaa aaagagaat attatagag 
      gatttagat aagggtatt aagcaactt aaggaaatt 
      ggcgacaaa atgaaaagt aaagacaca gttgttgat 
      gatgttaag aaaaaagca aaggggtct aaagataag 
      ggctaa
    mRNA sequence
    • >TC013920 RA
      gaaaatgtg tcaaaattg ggctgttcc gtgaagatg 
      gtcacgtca cgccgcacg cattctgac aaaaataat 
      gtgtgtaag tagcttaat ttaagcggc gaaagcgtg 
      cggttgatg cccgcactt ggacaactt gcgtgctca 
      ttttgaggt tatatttac aaattatat catggcaaa 
      tgtaattgc aagaaggtt tttcaaccg gaatttatt 
      taaatttaa tgaactggc tcaactggc aacacacag 
      cagatgcac ggctactgt catcataaa ttttaatca 
      taattatgg tactcccaa cccaaggaa tcacaagtt 
      gctccaacc gttcaaaac tacggaaat tcagcactt 
      ttattcaga cttgtccaa aaacaagaa aaaaccttc 
      gatatttca gaggtttaa gcagatgta taaggagta 
      ctggtatgt ccttctccc ggttcatct tgtcacttc 
      tgcagcatg gtttggtgg tttttatta tttagccaa 
      aagtggtgt ggatattgt gggaatttt ggaatcatg 
      gaacattag tgagaggat tgtgaatcc cttgagaga 
      ctcaagtat gggctatgt cgctgttgc ttacgctct 
      ttataaaat agcgacgcc tgcgaggta cacagtcac 
      gttaggtgg aaccacgat ttcaatcaa ttatctgaa 
      aaagtgggg ctacatcaa accagttcc tagtaaaga 
      gaggctcaa agaaatgta ctttgaaca gaaagataa 
      cttgttgga gacagtaag agagaccaa ggaaggttt 
      gaaagacaa aaaagagaa tattataga ggatttaga 
      taagggtat taagcaact taaggaaat tggcgacaa 
      aatgaaaag taaagacac agttgttga tgatgttaa 
      gaaaaaagc aaaggggtc taaagataa gggctaatt 
      attatgttt atatagttt ataagattt aaaatatat 
      tttatttag tgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY