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Gene information
General information
TC013887 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2207714..2213229
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013887
    iBeetle screen iB_09047

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013887 RA
      MQSFTCRSF KTIKKIPVI VRFYAVDNR EAVRVRFAP 
      SPTGFLHLG GLRTALYNY FFAKSCNGA FILRIEDTD 
      QSRLVEGAI EQLQHDLNW AGIQIDEGP SSGGSHGPY 
      IQSQRLHIY REHVTTLLN NGSAYHCFC TEKRLELLR 
      REAIRIREI PKYDNRCRS LKPEEVEEK LAQGIQSCV 
      RFKLAPGVQ NYADLIYGS LNYDVAANE GDPVIVKSD 
      GFPTYHFAN VVDDHLMKV SHVLRGVEW QISTPKHIM 
      MYRAFGWEP PLFGHLPLL MNPDGSKLS KRQGDIRIG 
      HYRDLGIFP LALINFITN SGGGFEKDS EQNVKPKCY 
      TMDELARQN QLTFGLELK RKLEDPNEE KKLVEEVGQ 
      MVRKSFAGN DLLQLDPQH VRYILHWSV NRITKLQDL 
      LSDDLKFIW VSPKSYQIS ENDLVPLGI FIEQLERNQ 
      TLDREHLNA FFKSFCKEH QLKFGNFMK TLRSILSGL 
      KEGPSVAEM VEILGKDNT IRRLQHCIK HNK
    • >TC013887 RB
      MQSFTCRSF KTIKKIPVI VRFYAVDNR EAVRVRFAP 
      SPTGFLHLG GLRTALYNY FFAKSCNGA FILRIEDTD 
      QSRLVEGAI EQLQHDLNW AGIQIDEGP SSGGSHGPY 
      IQSQRLHIY REHVTTLLN NGSAYHCFC TEKRLELLR 
      REAIRIREI PKYDNRCRS LKPEEVEEK LAQGIQSCV 
      RFKLAPGVQ NYADLIYGS LNYDVAANE GDPVIVKSD 
      GFPTYHFAN VVDDHLMKV SHVLRGVEW QISTPKHIM 
      MYRAFGWEP PLFGHLPLL MNPDGSKLS KRQGDIRIG 
      HYRDLGIFP LALINFITN SGGGFEKDS EQNVKPKCY 
      TMDELARQF GVSRINSHS GKLMPERLY EFNRLELKR 
      KLEDPNEEK KLVEEVGQM VRKSFAGND LLQLDPQHV 
      RYILHWSVN RITKLQDLL SDDLKFIWV SPKSYQISE 
      NDLVPLGIF IEQLERNQT LDREHLNAF FKSFCKEHQ 
      LKFGNFMKT LRSILSGLK EGPSVAEMV EILGKDNTI 
      RRLQHCIKH NK
    CDS sequence
    • >TC013887 RA
      atgcaatct ttcacttgt agatcgttt aaaactatt 
      aagaaaatc ccagtaatt gtccgattt tacgctgta 
      gacaacagg gaagcggtt agggtacgt tttgcgcca 
      agtcctaca ggcttcttg cacttgggg ggcttaaga 
      acagcgctt tataattac ttttttgcc aagtcgtgc 
      aacggtgcg tttatttta cgtattgaa gatacggat 
      caaagccgt ctggttgag ggggccatt gaacagctg 
      caacatgac ttaaattgg gccggaatt caaattgac 
      gaggggcca agtagcggg ggcagccac ggcccttat 
      atccaatcc caaagatta cacatttat agggagcat 
      gttacgact ttactaaac aacggctcg gcttatcac 
      tgcttttgc accgaaaag cggttagag ttgttacgt 
      agagaggcc atcagaatt agggaaatc cccaagtat 
      gacaacagg tgtcgcagt ttaaaacct gaagaagtt 
      gaggaaaaa ttggcccaa ggaattcaa tcatgtgtt 
      agattcaaa ttggcaccg ggcgttcaa aattatgct 
      gatttgatt tacggtagt ttaaattac gatgttgcc 
      gccaacgag ggcgaccct gtgatagta aaatccgac 
      ggttttccc acttaccac tttgccaac gtggttgac 
      gaccattta atgaaagtt tcgcacgtt ttgcgaggg 
      gttgagtgg cagatttca accccaaaa cacataatg 
      atgtataga gccttcggc tgggaaccc ccgctgttt 
      ggacacttg cctctttta atgaaccct gatggttcg 
      aaacttagc aaaagacag ggggatatc cggattggt 
      cattataga gatctaggg atatttccg ttagctttg 
      attaatttt attacaaat tcgggcggc gggttcgag 
      aaggatagt gaacaaaat gtgaaacca aaatgttac 
      actatggac gagttggca cgacagaat caactcaca 
      ttcggacta gaactgaag cgtaaactt gaagaccca 
      aacgaggag aaaaaatta gttgaagaa gtgggtcaa 
      atggtgagg aaatctttc gcagggaat gatttgctt 
      caattggat ccacaacac gtgaggtat atcctccac 
      tggtcagtt aatcggatc acaaaactg caagatttg 
      ctatcagat gatttgaaa tttatctgg gtttcccct 
      aaaagctac caaatttct gaaaatgat ttagtccct 
      cttgggatt ttcattgaa cagttggag cggaaccaa 
      acgttggac cgagaacat ttgaacgca tttttcaaa 
      agtttttgc aaggagcat cagctcaag tttgggaac 
      ttcatgaag actttgagg tcaatttta agcggattg 
      aaagaggga ccaagtgta gccgaaatg gtggaaatt 
      ttgggcaag gacaacacc atcagacga ctccagcat 
      tgcataaaa cacaacaaa tga
    • >TC013887 RB
      atgcaatct ttcacttgt agatcgttt aaaactatt 
      aagaaaatc ccagtaatt gtccgattt tacgctgta 
      gacaacagg gaagcggtt agggtacgt tttgcgcca 
      agtcctaca ggcttcttg cacttgggg ggcttaaga 
      acagcgctt tataattac ttttttgcc aagtcgtgc 
      aacggtgcg tttatttta cgtattgaa gatacggat 
      caaagccgt ctggttgag ggggccatt gaacagctg 
      caacatgac ttaaattgg gccggaatt caaattgac 
      gaggggcca agtagcggg ggcagccac ggcccttat 
      atccaatcc caaagatta cacatttat agggagcat 
      gttacgact ttactaaac aacggctcg gcttatcac 
      tgcttttgc accgaaaag cggttagag ttgttacgt 
      agagaggcc atcagaatt agggaaatc cccaagtat 
      gacaacagg tgtcgcagt ttaaaacct gaagaagtt 
      gaggaaaaa ttggcccaa ggaattcaa tcatgtgtt 
      agattcaaa ttggcaccg ggcgttcaa aattatgct 
      gatttgatt tacggtagt ttaaattac gatgttgcc 
      gccaacgag ggcgaccct gtgatagta aaatccgac 
      ggttttccc acttaccac tttgccaac gtggttgac 
      gaccattta atgaaagtt tcgcacgtt ttgcgaggg 
      gttgagtgg cagatttca accccaaaa cacataatg 
      atgtataga gccttcggc tgggaaccc ccgctgttt 
      ggacacttg cctctttta atgaaccct gatggttcg 
      aaacttagc aaaagacag ggggatatc cggattggt 
      cattataga gatctaggg atatttccg ttagctttg 
      attaatttt attacaaat tcgggcggc gggttcgag 
      aaggatagt gaacaaaat gtgaaacca aaatgttac 
      actatggac gagttggca cgacagttt ggtgtgtct 
      agaatcaac tcacattcg ggtaaatta atgccagaa 
      aggctttac gagttcaat agactagaa ctgaagcgt 
      aaacttgaa gacccaaac gaggagaaa aaattagtt 
      gaagaagtg ggtcaaatg gtgaggaaa tctttcgca 
      gggaatgat ttgcttcaa ttggatcca caacacgtg 
      aggtatatc ctccactgg tcagttaat cggatcaca 
      aaactgcaa gatttgcta tcagatgat ttgaaattt 
      atctgggtt tcccctaaa agctaccaa atttctgaa 
      aatgattta gtccctctt gggattttc attgaacag 
      ttggagcgg aaccaaacg ttggaccga gaacatttg 
      aacgcattt ttcaaaagt ttttgcaag gagcatcag 
      ctcaagttt gggaacttc atgaagact ttgaggtca 
      attttaagc ggattgaaa gagggacca agtgtagcc 
      gaaatggtg gaaattttg ggcaaggac aacaccatc 
      agacgactc cagcattgc ataaaacac aacaaatga
    mRNA sequence
    • >TC013887 RA
      gagaaagtg aaaagaaag tgcaagttt attcaactg 
      tgggtgacg aaattgcgc ccaattccc aattttttc 
      ccaaaccaa acatgaaga agcccattt ttacataac 
      ctccaattt tccacaatg caatctttc acttgtaga 
      tcgtttaaa actattaag aaaatccca gtaattgtc 
      cgattttac gctgtagac aacagggaa gcggttagg 
      gtacgtttt gcgccaagt cctacaggc ttcttgcac 
      ttggggggc ttaagaaca gcgctttat aattacttt 
      tttgccaag tcgtgcaac ggtgcgttt attttacgt 
      attgaagat acggatcaa agccgtctg gttgagggg 
      gccattgaa cagctgcaa catgactta aattgggcc 
      ggaattcaa attgacgag gggccaagt agcgggggc 
      agccacggc ccttatatc caatcccaa agattacac 
      atttatagg gagcatgtt acgacttta ctaaacaac 
      ggctcggct tatcactgc ttttgcacc gaaaagcgg 
      ttagagttg ttacgtaga gaggccatc agaattagg 
      gaaatcccc aagtatgac aacaggtgt cgcagttta 
      aaacctgaa gaagttgag gaaaaattg gcccaagga 
      attcaatca tgtgttaga ttcaaattg gcaccgggc 
      gttcaaaat tatgctgat ttgatttac ggtagttta 
      aattacgat gttgccgcc aacgagggc gaccctgtg 
      atagtaaaa tccgacggt tttcccact taccacttt 
      gccaacgtg gttgacgac catttaatg aaagtttcg 
      cacgttttg cgaggggtt gagtggcag atttcaacc 
      ccaaaacac ataatgatg tatagagcc ttcggctgg 
      gaacccccg ctgtttgga cacttgcct cttttaatg 
      aaccctgat ggttcgaaa cttagcaaa agacagggg 
      gatatccgg attggtcat tatagagat ctagggata 
      tttccgtta gctttgatt aattttatt acaaattcg 
      ggcggcggg ttcgagaag gatagtgaa caaaatgtg 
      aaaccaaaa tgttacact atggacgag ttggcacga 
      cagaatcaa ctcacattc ggactagaa ctgaagcgt 
      aaacttgaa gacccaaac gaggagaaa aaattagtt 
      gaagaagtg ggtcaaatg gtgaggaaa tctttcgca 
      gggaatgat ttgcttcaa ttggatcca caacacgtg 
      aggtatatc ctccactgg tcagttaat cggatcaca 
      aaactgcaa gatttgcta tcagatgat ttgaaattt 
      atctgggtt tcccctaaa agctaccaa atttctgaa 
      aatgattta gtccctctt gggattttc attgaacag 
      ttggagcgg aaccaaacg ttggaccga gaacatttg 
      aacgcattt ttcaaaagt ttttgcaag gagcatcag 
      ctcaagttt gggaacttc atgaagact ttgaggtca 
      attttaagc ggattgaaa gagggacca agtgtagcc 
      gaaatggtg gaaattttg ggcaaggac aacaccatc 
      agacgactc cagcattgc ataaaacac aacaaatga 
      gataaatat atttaatat ctttcattg taatacaat 
      cataacaaa aaagattaa aaaacttag taaaaaatt 
      caatttagc atgagttcg tccttttcc tagtaaaat 
      aatttacat acatatttt actcaagag ttgaggttc 
      aaaaaccaa acacaagtt gcagaaggc attcattca 
      agcaatggt gtgaagtaa caaactatt gcttgaaga 
      gtcttgtgt aacaattta ctatttttc ctattttgc 
      gtccaaaga cgcgaaata ggagaaatg taacaatga 
      acgaaaaga gtcgtattg ctggccatt ttattacaa 
      gtgactgca acattatat cacagaagt tcaacagca 
      gtcttaggt aaaaaaata caggcggat tttgagagc 
      gtttttaag tggcttaat aaagttctg gaaatccgc 
      ccaaataat acgaattcg gtgaacaga agacaatca 
      cagggctga agaaagtga taggcggtc tcgagcggt 
      ggcggttgt ggtgtttaa aactgggcc acttccgcc 
      accgatcag tgtttggga aaatttggt ccacttgtt 
      tgtgtttct ttccgtttg atcaatgat tgtacgaag 
      tggagaaat caggagaaa caaacaacg tgaagaaag 
      gaatgcaaa atatcagtt tgccgaaat ttcctaaat 
      ctagacatt tgtacatac gggaatgat cgtaaggct 
      acaacatat ttgtgctaa gtaatacat aacacgttc 
      acaggaagt ttggttgaa gtgcgctgt tcatggcag 
      tcagtttac cttcttgat aggagtacc aataaatct 
      gtggttttg ttacagcta ctctaagaa atagttttc 
      cgataa
    • >TC013887 RB
      gagaaagtg aaaagaaag tgcaagttt attcaactg 
      tgggtgacg aaattgcgc ccaattccc aattttttc 
      ccaaaccaa acatgaaga agcccattt ttacataac 
      ctccaattt tccacaatg caatctttc acttgtaga 
      tcgtttaaa actattaag aaaatccca gtaattgtc 
      cgattttac gctgtagac aacagggaa gcggttagg 
      gtacgtttt gcgccaagt cctacaggc ttcttgcac 
      ttggggggc ttaagaaca gcgctttat aattacttt 
      tttgccaag tcgtgcaac ggtgcgttt attttacgt 
      attgaagat acggatcaa agccgtctg gttgagggg 
      gccattgaa cagctgcaa catgactta aattgggcc 
      ggaattcaa attgacgag gggccaagt agcgggggc 
      agccacggc ccttatatc caatcccaa agattacac 
      atttatagg gagcatgtt acgacttta ctaaacaac 
      ggctcggct tatcactgc ttttgcacc gaaaagcgg 
      ttagagttg ttacgtaga gaggccatc agaattagg 
      gaaatcccc aagtatgac aacaggtgt cgcagttta 
      aaacctgaa gaagttgag gaaaaattg gcccaagga 
      attcaatca tgtgttaga ttcaaattg gcaccgggc 
      gttcaaaat tatgctgat ttgatttac ggtagttta 
      aattacgat gttgccgcc aacgagggc gaccctgtg 
      atagtaaaa tccgacggt tttcccact taccacttt 
      gccaacgtg gttgacgac catttaatg aaagtttcg 
      cacgttttg cgaggggtt gagtggcag atttcaacc 
      ccaaaacac ataatgatg tatagagcc ttcggctgg 
      gaacccccg ctgtttgga cacttgcct cttttaatg 
      aaccctgat ggttcgaaa cttagcaaa agacagggg 
      gatatccgg attggtcat tatagagat ctagggata 
      tttccgtta gctttgatt aattttatt acaaattcg 
      ggcggcggg ttcgagaag gatagtgaa caaaatgtg 
      aaaccaaaa tgttacact atggacgag ttggcacga 
      cagtttggt gtgtctaga atcaactca cattcgggt 
      aaattaatg ccagaaagg ctttacgag ttcaataga 
      ctagaactg aagcgtaaa cttgaagac ccaaacgag 
      gagaaaaaa ttagttgaa gaagtgggt caaatggtg 
      aggaaatct ttcgcaggg aatgatttg cttcaattg 
      gatccacaa cacgtgagg tatatcctc cactggtca 
      gttaatcgg atcacaaaa ctgcaagat ttgctatca 
      gatgatttg aaatttatc tgggtttcc cctaaaagc 
      taccaaatt tctgaaaat gatttagtc cctcttggg 
      attttcatt gaacagttg gagcggaac caaacgttg 
      gaccgagaa catttgaac gcatttttc aaaagtttt 
      tgcaaggag catcagctc aagtttggg aacttcatg 
      aagactttg aggtcaatt ttaagcgga ttgaaagag 
      ggaccaagt gtagccgaa atggtggaa attttgggc 
      aaggacaac accatcaga cgactccag cattgcata 
      aaacacaac aaatgagat aaatatatt taatatctt 
      tcattgtaa tacaatcat aacaaaaaa gattaaaaa 
      acttagtaa aaaattcaa tttagcatg agttcgtcc 
      ttttcctag taaaataat ttacataca tattttact 
      caagagttg aggttcaaa aaccaaaca caagttgca 
      gaaggcatt cattcaagc aatggtgtg aagtaacaa 
      actattgct tgaagagtc ttgtgtaac aatttacta 
      tttttccta ttttgcgtc caaagacgc gaaatagga 
      gaaatgtaa caatgaacg aaaagagtc gtattgctg 
      gccatttta ttacaagtg actgcaaca ttatatcac 
      agaagttca acagcagtc ttaggtaaa aaaatacag 
      gcggatttt gagagcgtt tttaagtgg cttaataaa 
      gttctggaa atccgccca aataatacg aattcggtg 
      aacagaaga caatcacag ggctgaaga aagtgatag 
      gcggtctcg agcggtggc ggttgtggt gtttaaaac 
      tgggccact tccgccacc gatcagtgt ttgggaaaa 
      tttggtcca cttgtttgt gtttctttc cgtttgatc 
      aatgattgt acgaagtgg agaaatcag gagaaacaa 
      acaacgtga agaaaggaa tgcaaaata tcagtttgc 
      cgaaatttc ctaaatcta gacatttgt acatacggg 
      aatgatcgt aaggctaca acatatttg tgctaagta 
      atacataac acgttcaca ggaagtttg gttgaagtg 
      cgctgttca tggcagtca gtttacctt cttgatagg 
      agtaccaat aaatctgtg gttttgtta cagctactc 
      taagaaata gttttccga taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY