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Gene information
General information
TC030342 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 1410020..1411635
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030342
    iBeetle screen iB_09046

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030342 RA
      MAFNESQDN FKLCFIAFN LSGLGPSSK PFLKILSYV 
      LYPWLCLLF VLVCVNIVF KHSNIWDIG EVTSSISIA 
      VMMVVRKTI LIKYSSVFA EIIELHSRF WDYGLFGKA 
      TETKIRKKV DFFKFILKC YIVSGITAT STRSIVPIF 
      DKNLTMPQD CWIPGNNSI VKHIIYAFQ VIFYAESIS 
      YFTFFDGFL LIVTANLQA QFILLQKAT GSINFETDS 
      EETAWKKLV KCCEHHKFL ISVHKKLNT LYSYFYLVT 
      YFLVITMGC VSLFVIFDK SSTFAQLLE SAITMVVLN 
      VMIAMICIC SSEIEIEAE KLLTQIYEV NWYETPNLK 
      IRKFILFWL MQAQVSVET KGAGLLVVN RSLMLQVQR 
      FSYSVSTLL KGMNE
    CDS sequence
    • >TC030342 RA
      atggcattt aacgaaagt caagacaat ttcaaattg 
      tgctttatt gcttttaac ttgagtggt ttagggcct 
      tcaagtaaa ccgttttta aaaattttg tcctacgtg 
      ctttatccc tggttatgc ctcttattt gttttggtg 
      tgcgttaac attgttttc aaacatagc aacatttgg 
      gacattggc gaagttacc tcttcgatc tccattgcc 
      gtaatgatg gttgttcga aagaccatt ttaattaaa 
      tatagctca gtgtttgcg gagattatc gaactgcat 
      tctcgattt tgggactat ggtcttttt ggaaaagct 
      actgaaaca aagattcga aaaaaagtg gactttttc 
      aagtttatt ttgaaatgc tacatcgtg tccggtatt 
      actgccaca tcaactcgt tctattgta ccgatattt 
      gacaagaac cttacaatg ccgcaagac tgttggatt 
      cctgggaac aattctatc gtaaaacat attatttat 
      gctttccaa gttatattt tatgcagaa agtattagt 
      tattttacc ttttttgat ggttttctt ttaattgtc 
      accgcaaat cttcaagca caattcatt ttgttgcaa 
      aaagcaact ggatctatt aattttgag accgactct 
      gaagaaacc gcttggaaa aaactagta aaatgttgt 
      gaacatcac aaattttta ataagcgtt cataagaag 
      ttaaacacc ttatattca tatttctat ttggttact 
      tatttcctg gtcattact atgggttgt gtgtcgctg 
      ttcgtcatt tttgacaag tcgtccact tttgctcaa 
      cttctagaa agcgcaatt actatggtt gtattaaat 
      gtcatgatt gccatgatc tgcatttgc agtagtgaa 
      atagagatt gaggctgaa aaactgctc actcaaatt 
      tacgaagta aattggtac gaaacaccg aacttaaaa 
      attcgcaag tttattctg ttttggctc atgcaagca 
      caggtttct gttgagaca aaaggcgca gggttgttg 
      gtagtaaac agatctctg atgcttcag gtccaacgt 
      ttttcatat tctgtatca acattatta aagggaatg 
      aatgaataa
    mRNA sequence
    • >TC030342 RA
      ttgttactt gctcaacgg caaaatggc atttaacga 
      aagtcaaga caatttcaa attgtgctt tattgcttt 
      taacttgag tggtttagg gccttcaag taaaccgtt 
      tttaaaaat tttgtccta cgtgcttta tccctggtt 
      atgcctctt atttgtttt ggtgtgcgt taacattgt 
      tttcaaaca tagcaacat ttgggacat tggcgaagt 
      tacctcttc gatctccat tgccgtaat gatggttgt 
      tcgaaagac cattttaat taaatatag ctcagtgtt 
      tgcggagat tatcgaact gcattctcg attttggga 
      ctatggtct ttttggaaa agctactga aacaaagat 
      tcgaaaaaa agtggactt tttcaagtt tattttgaa 
      atgctacat cgtgtccgg tattactgc cacatcaac 
      tcgttctat tgtaccgat atttgacaa gaaccttac 
      aatgccgca agactgttg gattcctgg gaacaattc 
      tatcgtaaa acatattat ttatgcttt ccaagttat 
      attttatgc agaaagtat tagttattt taccttttt 
      tgatggttt tcttttaat tgtcaccgc aaatcttca 
      agcacaatt cattttgtt gcaaaaagc aactggatc 
      tattaattt tgagaccga ctctgaaga aaccgcttg 
      gaaaaaact agtaaaatg ttgtgaaca tcacaaatt 
      tttaataag cgttcataa gaagttaaa caccttata 
      ttcatattt ctatttggt tacttattt cctggtcat 
      tactatggg ttgtgtgtc gctgttcgt catttttga 
      caagtcgtc cacttttgc tcaacttct agaaagcgc 
      aattactat ggttgtatt aaatgtcat gattgccat 
      gatctgcat ttgcagtag tgaaataga gattgaggc 
      tgaaaaact gctcactca aatttacga agtaaattg 
      gtacgaaac accgaactt aaaaattcg caagtttat 
      tctgttttg gctcatgca agcacaggt ttctgttga 
      gacaaaagg cgcagggtt gttggtagt aaacagatc 
      tctgatgct tcaggtcca acgtttttc atattctgt 
      atcaacatt attaaaggg aatgaatga ataagaatt 
      ataacacta agacaactt atggcaatt tttaaaaca 
      taaatactg ttgtaaatg tagtttttt gcacttctt 
      ttaaaaggc actagggtt cagcttcaa cattaattt 
      tttagaaaa cagttttaa gtcgacaat atttatgca 
      tttattcta atttttata acacttgtt cttataata 
      tttatgtag ttagtgaaa gcacttaat aaagacttt 
      gtacactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY