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Gene information
General information
TC034675 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11153007..11162624
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034675
    iBeetle screen iB_09040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034675 RA
      MKKEYGAIN SCEYYDSKL LLTKSSKND KDEKQRLLD 
      SDAPDVSLD LDSASGEDF SEDPTLNRK LEHPTSNFD 
      TMIHLLKGN IGTGILAMP DAFRNAGWV VGLFGTMFM 
      GFICTHCMH MLVACAHEL CRRTQKPAL SFDEVVENA 
      FKTGPQPLQ RFSQIAKTL INLFLCITQ LGFCCVYFV 
      FVAANLHDV IKHYFFDIS VHWYLVILL IPMVLLNFV 
      KSLKYLTPA SLFASILTC SGLVITFFY MLQDLPDTS 
      TVQAFSSWS QLPLYFGTA IYAFEGIGV ILPLENNMK 
      SPQDFGGWT GVLNTGMVI VATLYTAVG FFGYLKYGD 
      QAVLGSVTL LLPPNELLA QSVRLMMAV AIFLSYSLQ 
      FYVPFNIVW PWVKDYFHS DKSKRLAEH ATRTILVFV 
      TFALAIAIP NLGAVISLV GAFSSSALA LIFPPLIEI 
      ITFWPDKLG KNNWILWKD LAIVLFGFV GFIIGSYVS 
      LLNILQPEM
    CDS sequence
    • >TC034675 RA
      atgaagaag gaatacggt gccatcaat tcgtgcgaa 
      tactacgac tccaaacta ctcctcacc aaatcatcg 
      aaaaatgac aaagacgag aagcaacgt ttactcgac 
      agcgacgcc cccgacgtc tccctggac ctggactcg 
      gcatcgggc gaagacttt tccgaagac ccaacgctc 
      aaccgaaaa ctggaacac ccaacttcg aactttgac 
      acgatgata cacttactg aaaggcaac attggaacg 
      ggaattttg gcaatgccc gacgctttc cgcaacgct 
      ggctgggtg gtgggtctg ttcgggacc atgttcatg 
      ggtttcatt tgcacacat tgcatgcac atgctggtg 
      gcttgcgcc catgaactg tgccgacga acgcaaaaa 
      cccgcactc agtttcgac gaagttgtt gaaaatgcg 
      ttcaaaacg ggcccacaa ccattgcaa cgtttcagt 
      caaattgca aagactttg attaatctt ttcttgtgc 
      atcacccag ttgggcttc tgttgcgtt tattttgtc 
      ttcgtggct gcgaatctc cacgatgtt atcaagcac 
      tattttttc gatattagt gtgcactgg tatctggtg 
      atacttctg attcctatg gttctactt aactttgtt 
      aaaagtttg aaatatttg actccggcg tcacttttt 
      gcatcaatc ttgacttgt agcggtttg gttataact 
      tttttctac atgcttcaa gacctacct gatacttcg 
      acagtgcaa gcgttttcg agttggtca caattgccg 
      ctttatttc gggactgcg atttatgct tttgagggc 
      attggtgtg attttaccg ttggaaaac aacatgaaa 
      tcgccgcaa gatttcggc ggttggacc ggagtttta 
      aacaccgga atggtaata gtggcaacc ctgtacaca 
      gctgtgggt tttttcggt tacttgaaa tatggggat 
      caagcggtt ttggggagc gttactctc ttattgcca 
      ccaaatgaa ttgttggca caatcggtc cggttgatg 
      atggcagtg gcgattttc ctctcctac agtctccag 
      ttttacgtt ccttttaac attgtgtgg ccttgggtc 
      aaggattat tttcattcg gataaatcg aaacgattg 
      gccgaacat gcaacgcga acaattcta gtttttgta 
      acgtttgcg ttagccatc gcgattccg aatttgggt 
      gctgttatt tcgctggtt ggggctttc agtagctca 
      gctttagct ttaattttc ccgcctttg atcgaaatt 
      atcactttc tggcccgac aaattaggc aaaaataac 
      tggatcttg tggaaagat ttggcgatt gttttgttc 
      ggctttgtc gggtttatt atcgggtcg tatgtcagt 
      ctgttgaat attttgcag ccggaaatg tga
    mRNA sequence
    • >TC034675 RA
      tcatatttt attatgtca gtcgaaaat tttaaatag 
      ttgtgtata aacaaaatt ggcgatttg tacaaaatg 
      gccttttga attaacgag atgaatatg tcaacggcg 
      actccgcta cagcccctc actgtaaat atgatgtac 
      ttttaaatg gttgctgca cgtagatgt cgcatgaga 
      actagaact gtaaatatg tattttttg cacttttta 
      aaccatttg agcacttat cacatgtat tggtggctg 
      caggctgtc actaacagg ccttatcac agtaccagt 
      tgaacaggt taaggatac gattttgca attaaatat 
      ttgggtgat aatatatcg ttaaaggtc aagtgtcaa 
      cacctattt ttctcacca atgaacaca tttttttaa 
      ttaaaaaat cgccccctg acccgtatt agaaatcta 
      agttttcca aaggaacgc ccccataaa tcaatgctt 
      gtgtttaaa gagtatttt caggcgtga gtcatattt 
      cgtcttttg gaatgctgt tgatttgcg caaattttt 
      attgcattt gatattgaa aagcacacg tgtttggtt 
      cttgtcaaa taatgattt tttattcaa cgataaagt 
      ttataaaca aatacgatt tattggtgg ggtgagggt 
      cagctgaat aatagcaat tttaaaaga gacgagcag 
      gcaacttaa tcagtagtc ctccactac tctgaccct 
      taatatctc aacaccagt ggttgaaat tttcgaaag 
      atgaagaag gaatacggt gccatcaat tcgtgcgaa 
      tactacgac tccaaacta ctcctcacc aaatcatcg 
      aaaaatgac aaagacgag aagcaacgt ttactcgac 
      agcgacgcc cccgacgtc tccctggac ctggactcg 
      gcatcgggc gaagacttt tccgaagac ccaacgctc 
      aaccgaaaa ctggaacac ccaacttcg aactttgac 
      acgatgata cacttactg aaaggcaac attggaacg 
      ggaattttg gcaatgccc gacgctttc cgcaacgct 
      ggctgggtg gtgggtctg ttcgggacc atgttcatg 
      ggtttcatt tgcacacat tgcatgcac atgctggtg 
      gcttgcgcc catgaactg tgccgacga acgcaaaaa 
      cccgcactc agtttcgac gaagttgtt gaaaatgcg 
      ttcaaaacg ggcccacaa ccattgcaa cgtttcagt 
      caaattgca aagactttg attaatctt ttcttgtgc 
      atcacccag ttgggcttc tgttgcgtt tattttgtc 
      ttcgtggct gcgaatctc cacgatgtt atcaagcac 
      tattttttc gatattagt gtgcactgg tatctggtg 
      atacttctg attcctatg gttctactt aactttgtt 
      aaaagtttg aaatatttg actccggcg tcacttttt 
      gcatcaatc ttgacttgt agcggtttg gttataact 
      tttttctac atgcttcaa gacctacct gatacttcg 
      acagtgcaa gcgttttcg agttggtca caattgccg 
      ctttatttc gggactgcg atttatgct tttgagggc 
      attggtgtg attttaccg ttggaaaac aacatgaaa 
      tcgccgcaa gatttcggc ggttggacc ggagtttta 
      aacaccgga atggtaata gtggcaacc ctgtacaca 
      gctgtgggt tttttcggt tacttgaaa tatggggat 
      caagcggtt ttggggagc gttactctc ttattgcca 
      ccaaatgaa ttgttggca caatcggtc cggttgatg 
      atggcagtg gcgattttc ctctcctac agtctccag 
      ttttacgtt ccttttaac attgtgtgg ccttgggtc 
      aaggattat tttcattcg gataaatcg aaacgattg 
      gccgaacat gcaacgcga acaattcta gtttttgta 
      acgtttgcg ttagccatc gcgattccg aatttgggt 
      gctgttatt tcgctggtt ggggctttc agtagctca 
      gctttagct ttaattttc ccgcctttg atcgaaatt 
      atcactttc tggcccgac aaattaggc aaaaataac 
      tggatcttg tggaaagat ttggcgatt gttttgttc 
      ggctttgtc gggtttatt atcgggtcg tatgtcagt 
      ctgttgaat attttgcag ccggaaatg tgaatttgg 
      ccccaaatc tcatgataa taatggtga caataatgt 
      atttatttt aatttaatt ctttgtata tataatttt 
      aagtattat taggtgagg tgttagcac acccctttc 
      ctgtgacat cagtattcc aaaatttgt ataaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY