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Gene information
General information
TC008487 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11419953..11425467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008487
    iBeetle screen iB_09036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008487 RA
      MESKIVNLL ETVLREIDE IKQKLNQVQ NQLEDQQSV 
EKAFGALHR HFAKKSKSQ NGITENTEK TETQNRILH 
EEIENKQKT VLETPQQKE EIENIIDLA LIKSRKITL 
DDLKSSLKY ELVHETHFP STRFKVLVK INGRVYFGE 
GGTLEKAKD SATRLFLQT LDYNYFKHT VKVSSTVLM 
NEEFANVNI DAQLIENYQ NCLEYVDSM LKYTLVEKT 
GPDHKPTFK MAAKVNEKT YFGLGGSKA KARMNVVEQ 
VLENLKNQL NEHKSKLED EPENIWIDV ISLKNKQIV 
PNYLKPLLK YKLVQDAPN NYQMAVKVN EKIYYGQGE 
SKTKARHNV TEKVLRFLL DQSHCPKPS FLLNKLHPN 
ATYRCTQLK NGSQLEFKV TITIGKSLF VGIGPSKKS 
AKKVAALSA LSGIQNCSK SLGNVSKPD IFRKLIEEK 
ICSLLDDKP ENSNYKVIA GIVMTRNET FLDVVAVSA 
GTKCISRAK ITKNGVNLN DMHAEILSR RCLIGYFYN 
QLELSVASK DSIFVPSGQ KFKLKDGIE FHLYINIAP 
CGDARIFCP QTTSKRVLR LKVEARGGT YSVKNEFLR 
NNFILMSCS DKICRWNVV GLQGALLSQ FLDPVYLKS 
IVLGNVIHE SHLQRAVYG RVKDTLPKL PSSFRLNLP 
SLFFLPSTD PRFTSGPSF AITWSKDEQ NCEIINART 
GQTDLGPSK YSKREFFKR YVHLCRKIP GQRKENVYK 
EAKSASLDY NVAKNCLNT AFEKAGLGN WITHSMEHD 
MFALN
    CDS sequence
    • >TC008487 RA
      atggaatca aaaatagta aatttactt gagacggtt 
ttgagagaa attgacgag attaagcaa aaactgaat 
caagtgcaa aatcaattg gaagaccaa caatctgtt 
gagaaagct ttcggagcc cttcatcga cactttgca 
aaaaaatcc aaatcacag aatggaatc accgaaaat 
acggaaaaa acagagacg caaaatcgc attttacac 
gaagagatt gaaaataaa cagaaaaca gttttagaa 
actccacaa caaaaagaa gaaattgaa aatataata 
gacttggct ttaatcaaa agtcgaaaa attacgttg 
gatgatttg aaatcttca ttaaaatac gaattggtc 
catgaaacg cactttccc tcaactaga ttcaaagtt 
ttggtaaaa ataaatggc agagtctat ttcggagaa 
ggtggcact ctagagaaa gccaaggat agtgcaact 
agattattt ttacaaaca ttagattac aattacttc 
aaacacaca gttaaggtg tcttcaact gtgttaatg 
aatgaggag tttgctaat gttaatata gacgcacag 
ttaatcgaa aactaccaa aattgtttg gaatatgtg 
gactcaatg cttaaatac acccttgtg gaaaaaaca 
ggacctgac cacaaacct acattcaaa atggcggcg 
aaagtgaat gaaaaaact tatttcggt ttgggtggt 
tccaaggca aaagccaga atgaatgtt gttgagcag 
gttttagaa aatttgaaa aatcagtta aatgaacac 
aaatctaaa ttggaggat gaacctgaa aatatctgg 
attgatgta atttcatta aaaaataaa caaattgtt 
ccaaactat ttaaaacca cttcttaaa tacaaactg 
gtccaagat gcacctaac aattaccaa atggcggtc 
aaagtgaat gaaaaaatc tactacggc caaggtgaa 
tcaaagaca aaagctcgg cataatgtc accgagaag 
gttttacga tttttattg gaccaatca cattgtccc 
aaaccttcg tttttactt aacaaattg caccccaac 
gcaacgtat cgatgcaca caactcaaa aatggctct 
cagcttgaa tttaaagtc actattaca attggaaag 
tcacttttt gtgggaatt ggtccgagc aagaaatcc 
gctaagaag gtggcagcg ctttcggca ttaagtggg 
attcagaac tgttcaaaa tcgctcgga aacgtgtca 
aaacccgac attttccga aaattaatc gaggagaaa 
atttgctca ttgttggat gacaaaccc gaaaattcc 
aattacaaa gtcatcgct gggattgtg atgactcga 
aacgagaca tttttagat gtcgtcgca gtttcggca 
ggcactaaa tgcatctcg cgggcaaaa atcaccaaa 
aatggtgtc aacctgaac gatatgcat gccgagatt 
ttatcacgt cggtgtttg attggctac ttttataac 
caattggaa ttaagtgtt gccagcaaa gattcaatt 
tttgttcca agtggacag aaattcaaa cttaaggat 
ggaatcgaa ttccacctt tacataaat atcgcaccg 
tgtggtgac gctcgaatt ttttgtcct caaacaacc 
tccaaacga gttttgagg ctcaaagtg gaagcgcgt 
ggaggaaca tattctgtt aaaaacgag tttctccga 
aataatttc atattaatg tcttgttcg gataaaatt 
tgccgatgg aacgttgta gggctccag ggggcgctt 
ttgtcacaa tttttggac ccggtttac ctcaagtcc 
attgtttta ggaaatgtg attcacgaa tcgcattta 
cagagagcg gtttatgga cgtgtcaaa gacactctt 
cccaaactc ccttcttct tttcgtcta aatctccct 
tcattattt ttcctccct tcgaccgat cctagattt 
acttccggt ccaagtttt gcaatcacg tggtcgaaa 
gatgagcaa aattgtgaa ataatcaat gccaggaca 
ggacaaacc gatttaggg ccctcgaaa tactcaaaa 
cgggagttt tttaaacgg tacgttcat ctgtgtcgg 
aaaattcca ggacaaagg aaagaaaat gtatacaag 
gaggcgaaa tctgcatcg ttggactat aatgtggcc 
aaaaactgt cttaacaca gcgtttgag aaggcgggg 
ctgggaaat tggattacg cattcaatg gaacatgac 
atgtttgca ctaaattaa
    mRNA sequence
    • >TC008487 RA
      agtctttat ctgcaaccg ttatcgtaa tcagttaca 
cgagaacac aaaggtttt catttttta tcgtaacag 
agtaattcc cctaaaata cgttgtgtg gctgtgaaa 
aaatggaat caaaaatag taaatttac ttgagacgg 
ttttgagag aaattgacg agattaagc aaaaactga 
atcaagtgc aaaatcaat tggaagacc aacaatctg 
ttgagaaag ctttcggag cccttcatc gacactttg 
caaaaaaat ccaaatcac agaatggaa tcaccgaaa 
atacggaaa aaacagaga cgcaaaatc gcattttac 
acgaagaga ttgaaaata aacagaaaa cagttttag 
aaactccac aacaaaaag aagaaattg aaaatataa 
tagacttgg ctttaatca aaagtcgaa aaattacgt 
tggatgatt tgaaatctt cattaaaat acgaattgg 
tccatgaaa cgcactttc cctcaacta gattcaaag 
ttttggtaa aaataaatg gcagagtct atttcggag 
aaggtggca ctctagaga aagccaagg atagtgcaa 
ctagattat ttttacaaa cattagatt acaattact 
tcaaacaca cagttaagg tgtcttcaa ctgtgttaa 
tgaatgagg agtttgcta atgttaata tagacgcac 
agttaatcg aaaactacc aaaattgtt tggaatatg 
tggactcaa tgcttaaat acacccttg tggaaaaaa 
caggacctg accacaaac ctacattca aaatggcgg 
cgaaagtga atgaaaaaa cttatttcg gtttgggtg 
gttccaagg caaaagcca gaatgaatg ttgttgagc 
aggttttag aaaatttga aaaatcagt taaatgaac 
acaaatcta aattggagg atgaacctg aaaatatct 
ggattgatg taatttcat taaaaaata aacaaattg 
ttccaaact atttaaaac cacttctta aatacaaac 
tggtccaag atgcaccta acaattacc aaatggcgg 
tcaaagtga atgaaaaaa tctactacg gccaaggtg 
aatcaaaga caaaagctc ggcataatg tcaccgaga 
aggttttac gatttttat tggaccaat cacattgtc 
ccaaacctt cgtttttac ttaacaaat tgcacccca 
acgcaacgt atcgatgca cacaactca aaaatggct 
ctcagcttg aatttaaag tcactatta caattggaa 
agtcacttt ttgtgggaa ttggtccga gcaagaaat 
ccgctaaga aggtggcag cgctttcgg cattaagtg 
ggattcaga actgttcaa aatcgctcg gaaacgtgt 
caaaacccg acattttcc gaaaattaa tcgaggaga 
aaatttgct cattgttgg atgacaaac ccgaaaatt 
ccaattaca aagtcatcg ctgggattg tgatgactc 
gaaacgaga catttttag atgtcgtcg cagtttcgg 
caggcacta aatgcatct cgcgggcaa aaatcacca 
aaaatggtg tcaacctga acgatatgc atgccgaga 
ttttatcac gtcggtgtt tgattggct acttttata 
accaattgg aattaagtg ttgccagca aagattcaa 
tttttgttc caagtggac agaaattca aacttaagg 
atggaatcg aattccacc tttacataa atatcgcac 
cgtgtggtg acgctcgaa ttttttgtc ctcaaacaa 
cctccaaac gagttttga ggctcaaag tggaagcgc 
gtggaggaa catattctg ttaaaaacg agtttctcc 
gaaataatt tcatattaa tgtcttgtt cggataaaa 
tttgccgat ggaacgttg tagggctcc agggggcgc 
ttttgtcac aatttttgg acccggttt acctcaagt 
ccattgttt taggaaatg tgattcacg aatcgcatt 
tacagagag cggtttatg gacgtgtca aagacactc 
ttcccaaac tcccttctt cttttcgtc taaatctcc 
cttcattat ttttcctcc cttcgaccg atcctagat 
ttacttccg gtccaagtt ttgcaatca cgtggtcga 
aagatgagc aaaattgtg aaataatca atgccagga 
caggacaaa ccgatttag ggccctcga aatactcaa 
aacgggagt tttttaaac ggtacgttc atctgtgtc 
ggaaaattc caggacaaa ggaaagaaa atgtataca 
aggaggcga aatctgcat cgttggact ataatgtgg 
ccaaaaact gtcttaaca cagcgtttg agaaggcgg 
ggctgggaa attggatta cgcattcaa tggaacatg 
acatgtttg cactaaatt aattttata tatttttta 
tgccagtcg gtttttttt cgagcaatt ctttttgtc 
tgtaatttc atctattat ctattatgt aattaatta 
tacctcaaa aactagagc tctaaaatt atattcttt 
ttctaaaga aacgctaaa ttgagttac ctatatctc 
aaaaactaa agttaatat aagaaa
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