Status
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Gene information
General information
TC008487 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11419953..11425467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008487
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008487 RA
      MESKIVNLL ETVLREIDE IKQKLNQVQ NQLEDQQSV 
      EKAFGALHR HFAKKSKSQ NGITENTEK TETQNRILH 
      EEIENKQKT VLETPQQKE EIENIIDLA LIKSRKITL 
      DDLKSSLKY ELVHETHFP STRFKVLVK INGRVYFGE 
      GGTLEKAKD SATRLFLQT LDYNYFKHT VKVSSTVLM 
      NEEFANVNI DAQLIENYQ NCLEYVDSM LKYTLVEKT 
      GPDHKPTFK MAAKVNEKT YFGLGGSKA KARMNVVEQ 
      VLENLKNQL NEHKSKLED EPENIWIDV ISLKNKQIV 
      PNYLKPLLK YKLVQDAPN NYQMAVKVN EKIYYGQGE 
      SKTKARHNV TEKVLRFLL DQSHCPKPS FLLNKLHPN 
      ATYRCTQLK NGSQLEFKV TITIGKSLF VGIGPSKKS 
      AKKVAALSA LSGIQNCSK SLGNVSKPD IFRKLIEEK 
      ICSLLDDKP ENSNYKVIA GIVMTRNET FLDVVAVSA 
      GTKCISRAK ITKNGVNLN DMHAEILSR RCLIGYFYN 
      QLELSVASK DSIFVPSGQ KFKLKDGIE FHLYINIAP 
      CGDARIFCP QTTSKRVLR LKVEARGGT YSVKNEFLR 
      NNFILMSCS DKICRWNVV GLQGALLSQ FLDPVYLKS 
      IVLGNVIHE SHLQRAVYG RVKDTLPKL PSSFRLNLP 
      SLFFLPSTD PRFTSGPSF AITWSKDEQ NCEIINART 
      GQTDLGPSK YSKREFFKR YVHLCRKIP GQRKENVYK 
      EAKSASLDY NVAKNCLNT AFEKAGLGN WITHSMEHD 
      MFALN
    CDS sequence
    • >TC008487 RA
      atggaatca aaaatagta aatttactt gagacggtt 
      ttgagagaa attgacgag attaagcaa aaactgaat 
      caagtgcaa aatcaattg gaagaccaa caatctgtt 
      gagaaagct ttcggagcc cttcatcga cactttgca 
      aaaaaatcc aaatcacag aatggaatc accgaaaat 
      acggaaaaa acagagacg caaaatcgc attttacac 
      gaagagatt gaaaataaa cagaaaaca gttttagaa 
      actccacaa caaaaagaa gaaattgaa aatataata 
      gacttggct ttaatcaaa agtcgaaaa attacgttg 
      gatgatttg aaatcttca ttaaaatac gaattggtc 
      catgaaacg cactttccc tcaactaga ttcaaagtt 
      ttggtaaaa ataaatggc agagtctat ttcggagaa 
      ggtggcact ctagagaaa gccaaggat agtgcaact 
      agattattt ttacaaaca ttagattac aattacttc 
      aaacacaca gttaaggtg tcttcaact gtgttaatg 
      aatgaggag tttgctaat gttaatata gacgcacag 
      ttaatcgaa aactaccaa aattgtttg gaatatgtg 
      gactcaatg cttaaatac acccttgtg gaaaaaaca 
      ggacctgac cacaaacct acattcaaa atggcggcg 
      aaagtgaat gaaaaaact tatttcggt ttgggtggt 
      tccaaggca aaagccaga atgaatgtt gttgagcag 
      gttttagaa aatttgaaa aatcagtta aatgaacac 
      aaatctaaa ttggaggat gaacctgaa aatatctgg 
      attgatgta atttcatta aaaaataaa caaattgtt 
      ccaaactat ttaaaacca cttcttaaa tacaaactg 
      gtccaagat gcacctaac aattaccaa atggcggtc 
      aaagtgaat gaaaaaatc tactacggc caaggtgaa 
      tcaaagaca aaagctcgg cataatgtc accgagaag 
      gttttacga tttttattg gaccaatca cattgtccc 
      aaaccttcg tttttactt aacaaattg caccccaac 
      gcaacgtat cgatgcaca caactcaaa aatggctct 
      cagcttgaa tttaaagtc actattaca attggaaag 
      tcacttttt gtgggaatt ggtccgagc aagaaatcc 
      gctaagaag gtggcagcg ctttcggca ttaagtggg 
      attcagaac tgttcaaaa tcgctcgga aacgtgtca 
      aaacccgac attttccga aaattaatc gaggagaaa 
      atttgctca ttgttggat gacaaaccc gaaaattcc 
      aattacaaa gtcatcgct gggattgtg atgactcga 
      aacgagaca tttttagat gtcgtcgca gtttcggca 
      ggcactaaa tgcatctcg cgggcaaaa atcaccaaa 
      aatggtgtc aacctgaac gatatgcat gccgagatt 
      ttatcacgt cggtgtttg attggctac ttttataac 
      caattggaa ttaagtgtt gccagcaaa gattcaatt 
      tttgttcca agtggacag aaattcaaa cttaaggat 
      ggaatcgaa ttccacctt tacataaat atcgcaccg 
      tgtggtgac gctcgaatt ttttgtcct caaacaacc 
      tccaaacga gttttgagg ctcaaagtg gaagcgcgt 
      ggaggaaca tattctgtt aaaaacgag tttctccga 
      aataatttc atattaatg tcttgttcg gataaaatt 
      tgccgatgg aacgttgta gggctccag ggggcgctt 
      ttgtcacaa tttttggac ccggtttac ctcaagtcc 
      attgtttta ggaaatgtg attcacgaa tcgcattta 
      cagagagcg gtttatgga cgtgtcaaa gacactctt 
      cccaaactc ccttcttct tttcgtcta aatctccct 
      tcattattt ttcctccct tcgaccgat cctagattt 
      acttccggt ccaagtttt gcaatcacg tggtcgaaa 
      gatgagcaa aattgtgaa ataatcaat gccaggaca 
      ggacaaacc gatttaggg ccctcgaaa tactcaaaa 
      cgggagttt tttaaacgg tacgttcat ctgtgtcgg 
      aaaattcca ggacaaagg aaagaaaat gtatacaag 
      gaggcgaaa tctgcatcg ttggactat aatgtggcc 
      aaaaactgt cttaacaca gcgtttgag aaggcgggg 
      ctgggaaat tggattacg cattcaatg gaacatgac 
      atgtttgca ctaaattaa
    mRNA sequence
    • >TC008487 RA
      agtctttat ctgcaaccg ttatcgtaa tcagttaca 
      cgagaacac aaaggtttt catttttta tcgtaacag 
      agtaattcc cctaaaata cgttgtgtg gctgtgaaa 
      aaatggaat caaaaatag taaatttac ttgagacgg 
      ttttgagag aaattgacg agattaagc aaaaactga 
      atcaagtgc aaaatcaat tggaagacc aacaatctg 
      ttgagaaag ctttcggag cccttcatc gacactttg 
      caaaaaaat ccaaatcac agaatggaa tcaccgaaa 
      atacggaaa aaacagaga cgcaaaatc gcattttac 
      acgaagaga ttgaaaata aacagaaaa cagttttag 
      aaactccac aacaaaaag aagaaattg aaaatataa 
      tagacttgg ctttaatca aaagtcgaa aaattacgt 
      tggatgatt tgaaatctt cattaaaat acgaattgg 
      tccatgaaa cgcactttc cctcaacta gattcaaag 
      ttttggtaa aaataaatg gcagagtct atttcggag 
      aaggtggca ctctagaga aagccaagg atagtgcaa 
      ctagattat ttttacaaa cattagatt acaattact 
      tcaaacaca cagttaagg tgtcttcaa ctgtgttaa 
      tgaatgagg agtttgcta atgttaata tagacgcac 
      agttaatcg aaaactacc aaaattgtt tggaatatg 
      tggactcaa tgcttaaat acacccttg tggaaaaaa 
      caggacctg accacaaac ctacattca aaatggcgg 
      cgaaagtga atgaaaaaa cttatttcg gtttgggtg 
      gttccaagg caaaagcca gaatgaatg ttgttgagc 
      aggttttag aaaatttga aaaatcagt taaatgaac 
      acaaatcta aattggagg atgaacctg aaaatatct 
      ggattgatg taatttcat taaaaaata aacaaattg 
      ttccaaact atttaaaac cacttctta aatacaaac 
      tggtccaag atgcaccta acaattacc aaatggcgg 
      tcaaagtga atgaaaaaa tctactacg gccaaggtg 
      aatcaaaga caaaagctc ggcataatg tcaccgaga 
      aggttttac gatttttat tggaccaat cacattgtc 
      ccaaacctt cgtttttac ttaacaaat tgcacccca 
      acgcaacgt atcgatgca cacaactca aaaatggct 
      ctcagcttg aatttaaag tcactatta caattggaa 
      agtcacttt ttgtgggaa ttggtccga gcaagaaat 
      ccgctaaga aggtggcag cgctttcgg cattaagtg 
      ggattcaga actgttcaa aatcgctcg gaaacgtgt 
      caaaacccg acattttcc gaaaattaa tcgaggaga 
      aaatttgct cattgttgg atgacaaac ccgaaaatt 
      ccaattaca aagtcatcg ctgggattg tgatgactc 
      gaaacgaga catttttag atgtcgtcg cagtttcgg 
      caggcacta aatgcatct cgcgggcaa aaatcacca 
      aaaatggtg tcaacctga acgatatgc atgccgaga 
      ttttatcac gtcggtgtt tgattggct acttttata 
      accaattgg aattaagtg ttgccagca aagattcaa 
      tttttgttc caagtggac agaaattca aacttaagg 
      atggaatcg aattccacc tttacataa atatcgcac 
      cgtgtggtg acgctcgaa ttttttgtc ctcaaacaa 
      cctccaaac gagttttga ggctcaaag tggaagcgc 
      gtggaggaa catattctg ttaaaaacg agtttctcc 
      gaaataatt tcatattaa tgtcttgtt cggataaaa 
      tttgccgat ggaacgttg tagggctcc agggggcgc 
      ttttgtcac aatttttgg acccggttt acctcaagt 
      ccattgttt taggaaatg tgattcacg aatcgcatt 
      tacagagag cggtttatg gacgtgtca aagacactc 
      ttcccaaac tcccttctt cttttcgtc taaatctcc 
      cttcattat ttttcctcc cttcgaccg atcctagat 
      ttacttccg gtccaagtt ttgcaatca cgtggtcga 
      aagatgagc aaaattgtg aaataatca atgccagga 
      caggacaaa ccgatttag ggccctcga aatactcaa 
      aacgggagt tttttaaac ggtacgttc atctgtgtc 
      ggaaaattc caggacaaa ggaaagaaa atgtataca 
      aggaggcga aatctgcat cgttggact ataatgtgg 
      ccaaaaact gtcttaaca cagcgtttg agaaggcgg 
      ggctgggaa attggatta cgcattcaa tggaacatg 
      acatgtttg cactaaatt aattttata tatttttta 
      tgccagtcg gtttttttt cgagcaatt ctttttgtc 
      tgtaatttc atctattat ctattatgt aattaatta 
      tacctcaaa aactagagc tctaaaatt atattcttt 
      ttctaaaga aacgctaaa ttgagttac ctatatctc 
      aaaaactaa agttaatat aagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY