| General information | |||
| TC008487 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 11419953..11425467 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC008487 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_09036 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC008487 RA
MESKIVNLL ETVLREIDE IKQKLNQVQ NQLEDQQSV EKAFGALHR HFAKKSKSQ NGITENTEK TETQNRILH EEIENKQKT VLETPQQKE EIENIIDLA LIKSRKITL DDLKSSLKY ELVHETHFP STRFKVLVK INGRVYFGE GGTLEKAKD SATRLFLQT LDYNYFKHT VKVSSTVLM NEEFANVNI DAQLIENYQ NCLEYVDSM LKYTLVEKT GPDHKPTFK MAAKVNEKT YFGLGGSKA KARMNVVEQ VLENLKNQL NEHKSKLED EPENIWIDV ISLKNKQIV PNYLKPLLK YKLVQDAPN NYQMAVKVN EKIYYGQGE SKTKARHNV TEKVLRFLL DQSHCPKPS FLLNKLHPN ATYRCTQLK NGSQLEFKV TITIGKSLF VGIGPSKKS AKKVAALSA LSGIQNCSK SLGNVSKPD IFRKLIEEK ICSLLDDKP ENSNYKVIA GIVMTRNET FLDVVAVSA GTKCISRAK ITKNGVNLN DMHAEILSR RCLIGYFYN QLELSVASK DSIFVPSGQ KFKLKDGIE FHLYINIAP CGDARIFCP QTTSKRVLR LKVEARGGT YSVKNEFLR NNFILMSCS DKICRWNVV GLQGALLSQ FLDPVYLKS IVLGNVIHE SHLQRAVYG RVKDTLPKL PSSFRLNLP SLFFLPSTD PRFTSGPSF AITWSKDEQ NCEIINART GQTDLGPSK YSKREFFKR YVHLCRKIP GQRKENVYK EAKSASLDY NVAKNCLNT AFEKAGLGN WITHSMEHD MFALN
- >TC008487 RA
atggaatca aaaatagta aatttactt gagacggtt ttgagagaa attgacgag attaagcaa aaactgaat caagtgcaa aatcaattg gaagaccaa caatctgtt gagaaagct ttcggagcc cttcatcga cactttgca aaaaaatcc aaatcacag aatggaatc accgaaaat acggaaaaa acagagacg caaaatcgc attttacac gaagagatt gaaaataaa cagaaaaca gttttagaa actccacaa caaaaagaa gaaattgaa aatataata gacttggct ttaatcaaa agtcgaaaa attacgttg gatgatttg aaatcttca ttaaaatac gaattggtc catgaaacg cactttccc tcaactaga ttcaaagtt ttggtaaaa ataaatggc agagtctat ttcggagaa ggtggcact ctagagaaa gccaaggat agtgcaact agattattt ttacaaaca ttagattac aattacttc aaacacaca gttaaggtg tcttcaact gtgttaatg aatgaggag tttgctaat gttaatata gacgcacag ttaatcgaa aactaccaa aattgtttg gaatatgtg gactcaatg cttaaatac acccttgtg gaaaaaaca ggacctgac cacaaacct acattcaaa atggcggcg aaagtgaat gaaaaaact tatttcggt ttgggtggt tccaaggca aaagccaga atgaatgtt gttgagcag gttttagaa aatttgaaa aatcagtta aatgaacac aaatctaaa ttggaggat gaacctgaa aatatctgg attgatgta atttcatta aaaaataaa caaattgtt ccaaactat ttaaaacca cttcttaaa tacaaactg gtccaagat gcacctaac aattaccaa atggcggtc aaagtgaat gaaaaaatc tactacggc caaggtgaa tcaaagaca aaagctcgg cataatgtc accgagaag gttttacga tttttattg gaccaatca cattgtccc aaaccttcg tttttactt aacaaattg caccccaac gcaacgtat cgatgcaca caactcaaa aatggctct cagcttgaa tttaaagtc actattaca attggaaag tcacttttt gtgggaatt ggtccgagc aagaaatcc gctaagaag gtggcagcg ctttcggca ttaagtggg attcagaac tgttcaaaa tcgctcgga aacgtgtca aaacccgac attttccga aaattaatc gaggagaaa atttgctca ttgttggat gacaaaccc gaaaattcc aattacaaa gtcatcgct gggattgtg atgactcga aacgagaca tttttagat gtcgtcgca gtttcggca ggcactaaa tgcatctcg cgggcaaaa atcaccaaa aatggtgtc aacctgaac gatatgcat gccgagatt ttatcacgt cggtgtttg attggctac ttttataac caattggaa ttaagtgtt gccagcaaa gattcaatt tttgttcca agtggacag aaattcaaa cttaaggat ggaatcgaa ttccacctt tacataaat atcgcaccg tgtggtgac gctcgaatt ttttgtcct caaacaacc tccaaacga gttttgagg ctcaaagtg gaagcgcgt ggaggaaca tattctgtt aaaaacgag tttctccga aataatttc atattaatg tcttgttcg gataaaatt tgccgatgg aacgttgta gggctccag ggggcgctt ttgtcacaa tttttggac ccggtttac ctcaagtcc attgtttta ggaaatgtg attcacgaa tcgcattta cagagagcg gtttatgga cgtgtcaaa gacactctt cccaaactc ccttcttct tttcgtcta aatctccct tcattattt ttcctccct tcgaccgat cctagattt acttccggt ccaagtttt gcaatcacg tggtcgaaa gatgagcaa aattgtgaa ataatcaat gccaggaca ggacaaacc gatttaggg ccctcgaaa tactcaaaa cgggagttt tttaaacgg tacgttcat ctgtgtcgg aaaattcca ggacaaagg aaagaaaat gtatacaag gaggcgaaa tctgcatcg ttggactat aatgtggcc aaaaactgt cttaacaca gcgtttgag aaggcgggg ctgggaaat tggattacg cattcaatg gaacatgac atgtttgca ctaaattaa
- >TC008487 RA
agtctttat ctgcaaccg ttatcgtaa tcagttaca cgagaacac aaaggtttt catttttta tcgtaacag agtaattcc cctaaaata cgttgtgtg gctgtgaaa aaatggaat caaaaatag taaatttac ttgagacgg ttttgagag aaattgacg agattaagc aaaaactga atcaagtgc aaaatcaat tggaagacc aacaatctg ttgagaaag ctttcggag cccttcatc gacactttg caaaaaaat ccaaatcac agaatggaa tcaccgaaa atacggaaa aaacagaga cgcaaaatc gcattttac acgaagaga ttgaaaata aacagaaaa cagttttag aaactccac aacaaaaag aagaaattg aaaatataa tagacttgg ctttaatca aaagtcgaa aaattacgt tggatgatt tgaaatctt cattaaaat acgaattgg tccatgaaa cgcactttc cctcaacta gattcaaag ttttggtaa aaataaatg gcagagtct atttcggag aaggtggca ctctagaga aagccaagg atagtgcaa ctagattat ttttacaaa cattagatt acaattact tcaaacaca cagttaagg tgtcttcaa ctgtgttaa tgaatgagg agtttgcta atgttaata tagacgcac agttaatcg aaaactacc aaaattgtt tggaatatg tggactcaa tgcttaaat acacccttg tggaaaaaa caggacctg accacaaac ctacattca aaatggcgg cgaaagtga atgaaaaaa cttatttcg gtttgggtg gttccaagg caaaagcca gaatgaatg ttgttgagc aggttttag aaaatttga aaaatcagt taaatgaac acaaatcta aattggagg atgaacctg aaaatatct ggattgatg taatttcat taaaaaata aacaaattg ttccaaact atttaaaac cacttctta aatacaaac tggtccaag atgcaccta acaattacc aaatggcgg tcaaagtga atgaaaaaa tctactacg gccaaggtg aatcaaaga caaaagctc ggcataatg tcaccgaga aggttttac gatttttat tggaccaat cacattgtc ccaaacctt cgtttttac ttaacaaat tgcacccca acgcaacgt atcgatgca cacaactca aaaatggct ctcagcttg aatttaaag tcactatta caattggaa agtcacttt ttgtgggaa ttggtccga gcaagaaat ccgctaaga aggtggcag cgctttcgg cattaagtg ggattcaga actgttcaa aatcgctcg gaaacgtgt caaaacccg acattttcc gaaaattaa tcgaggaga aaatttgct cattgttgg atgacaaac ccgaaaatt ccaattaca aagtcatcg ctgggattg tgatgactc gaaacgaga catttttag atgtcgtcg cagtttcgg caggcacta aatgcatct cgcgggcaa aaatcacca aaaatggtg tcaacctga acgatatgc atgccgaga ttttatcac gtcggtgtt tgattggct acttttata accaattgg aattaagtg ttgccagca aagattcaa tttttgttc caagtggac agaaattca aacttaagg atggaatcg aattccacc tttacataa atatcgcac cgtgtggtg acgctcgaa ttttttgtc ctcaaacaa cctccaaac gagttttga ggctcaaag tggaagcgc gtggaggaa catattctg ttaaaaacg agtttctcc gaaataatt tcatattaa tgtcttgtt cggataaaa tttgccgat ggaacgttg tagggctcc agggggcgc ttttgtcac aatttttgg acccggttt acctcaagt ccattgttt taggaaatg tgattcacg aatcgcatt tacagagag cggtttatg gacgtgtca aagacactc ttcccaaac tcccttctt cttttcgtc taaatctcc cttcattat ttttcctcc cttcgaccg atcctagat ttacttccg gtccaagtt ttgcaatca cgtggtcga aagatgagc aaaattgtg aaataatca atgccagga caggacaaa ccgatttag ggccctcga aatactcaa aacgggagt tttttaaac ggtacgttc atctgtgtc ggaaaattc caggacaaa ggaaagaaa atgtataca aggaggcga aatctgcat cgttggact ataatgtgg ccaaaaact gtcttaaca cagcgtttg agaaggcgg ggctgggaa attggatta cgcattcaa tggaacatg acatgtttg cactaaatt aattttata tatttttta tgccagtcg gtttttttt cgagcaatt ctttttgtc tgtaatttc atctattat ctattatgt aattaatta tacctcaaa aactagagc tctaaaatt atattcttt ttctaaaga aacgctaaa ttgagttac ctatatctc aaaaactaa agttaatat aagaaa
