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Gene information
General information
TC007385 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5275368..5277163
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007385
    iBeetle screen iB_09033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007385 RA
      MWWTPFMGE NERITACPN HNCILTKDR KYLNNTNLQ 
      AFLFYGSKF NISDLPKRK DTVWALFHE ESPRNVALF 
      LHKRTFEVF DIAATFSRH SDFPLTLQY LEKLSLLTD 
      LQYFMDVKE KNKLLNSIA PVLYIQSDC DTPIERDNY 
      VKELMKYIK VDSFGKCLK NKEFPPQVS EIYSLDLYN 
      EQLLHFIAK YKFVIAFEN AICDDYITE KLWRPLIAG 
      SVPVYLGSP TVEDWLPNK NSAILAKNF HSPADLATH 
      ITKINDDDF LYEMYQGHK FGEIENSFL KSVLARGPF 
      GIATDQEHP ISAFECFVC EQIHEGFEK RVSDSVYNC 
      QRPNPGNSW EYFWQLGDC QSRALVNFV KNGEMIEQE 
      VC
    CDS sequence
    • >TC007385 RA
      atgtggtgg accccattc atgggagaa aacgaacgg 
      attaccgcc tgtcccaac cataattgt atattaacc 
      aaggacagg aaatattta aacaataca aatttgcaa 
      gctttttta ttttatgga agcaagttc aatattagt 
      gacttgccg aaacggaaa gacactgtt tgggccctt 
      tttcacgag gaatcgccc agaaatgta gcacttttc 
      ttgcacaag agaactttc gaggtgttt gatattgcg 
      gcgactttt agcagacac agtgatttt ccgctgact 
      ttgcaatat ttagaaaaa ttaagtcta cttaccgac 
      ttgcagtat ttcatggat gtgaaagaa aaaaataaa 
      ttactcaat agtatagct ccagtattg tacatacag 
      tcggactgt gatacccca atagaaaga gataattac 
      gtgaaagaa cttatgaaa tatattaaa gttgattca 
      tttgggaaa tgcctaaaa aacaaggaa tttccacca 
      caagtttca gaaatatat tccctggat ttatacaac 
      gaacaactg ctccacttt atagccaaa tacaagttt 
      gtgattgct ttcgaaaat gccatttgc gacgactac 
      atcactgaa aaattgtgg cgtccactg atagcaggt 
      tcagtccca gtatattta ggatcgccc actgttgag 
      gactggttg cccaacaaa aactcggca atcctagca 
      aaaaatttc cacagtccg gcagacctt gccacccac 
      attacgaaa atcaacgac gatgatttt ttatacgag 
      atgtaccaa ggacacaag tttggtgaa attgagaac 
      agttttctt aaaagtgtc ttagctagg ggtccgttt 
      gggattgcg acagatcag gagcacccc atttcagcg 
      tttgaatgt tttgtgtgt gaacagatt cacgaagga 
      tttgaaaaa cgtgtctca gactctgtt tataattgt 
      cagagaccg aatccggga aacagttgg gagtacttc 
      tggcaattg ggggattgc cagtccagg gccttggtc 
      aattttgtt aaaaacggc gaaatgata gagcaagaa 
      gtttgttga
    mRNA sequence
    • >TC007385 RA
      cgaggtcaa aatctgtta ctgtgttaa ctttgccca 
      acttttcct cttttgagt caaaaaagg tgtatagtt 
      atttacaac acgcgtttg gttgtgttt atcattttt 
      tccattata aatatgtac tttatgcaa aattttgtg 
      taaatttga gtgcaatgt tgccaaatc actaactca 
      aaatgccat tcataaaaa atcgtattt atacaaacg 
      ataaactct tccgtccgt aaacaaacc ggaaatgtt 
      tattctcgt atttttcct cctcaattt attttaacg 
      atgttaggt tatattcaa aaactcaaa ataaaaaaa 
      atattttgc atcatatta gcatcagga acatccttt 
      tatcttctc cagtatcca ataatcatg tggtggacc 
      ccattcatg ggagaaaac gaacggatt accgcctgt 
      cccaaccat aattgtata ttaaccaag gacaggaaa 
      tatttaaac aatacaaat ttgcaagct tttttattt 
      tatggaagc aagttcaat attagtgac ttgccgaaa 
      cggaaagac actgtttgg gcccttttt cacgaggaa 
      tcgcccaga aatgtagca cttttcttg cacaagaga 
      actttcgag gtgtttgat attgcggcg acttttagc 
      agacacagt gattttccg ctgactttg caatattta 
      gaaaaatta agtctactt accgacttg cagtatttc 
      atggatgtg aaagaaaaa aataaatta ctcaatagt 
      atagctcca gtattgtac atacagtcg gactgtgat 
      accccaata gaaagagat aattacgtg aaagaactt 
      atgaaatat attaaagtt gattcattt gggaaatgc 
      ctaaaaaac aaggaattt ccaccacaa gtttcagaa 
      atatattcc ctggattta tacaacgaa caactgctc 
      cactttata gccaaatac aagtttgtg attgctttc 
      gaaaatgcc atttgcgac gactacatc actgaaaaa 
      ttgtggcgt ccactgata gcaggttca gtcccagta 
      tatttagga tcgcccact gttgaggac tggttgccc 
      aacaaaaac tcggcaatc ctagcaaaa aatttccac 
      agtccggca gaccttgcc acccacatt acgaaaatc 
      aacgacgat gatttttta tacgagatg taccaagga 
      cacaagttt ggtgaaatt gagaacagt tttcttaaa 
      agtgtctta gctaggggt ccgtttggg attgcgaca 
      gatcaggag caccccatt tcagcgttt gaatgtttt 
      gtgtgtgaa cagattcac gaaggattt gaaaaacgt 
      gtctcagac tctgtttat aattgtcag agaccgaat 
      ccgggaaac agttgggag tacttctgg caattgggg 
      gattgccag tccagggcc ttggtcaat tttgttaaa 
      aacggcgaa atgatagag caagaagtt tgttgagta 
      attgataac aactacaaa ttgtatatt tttattaag 
      tgacataac tctgctact aaaaaaact atgtgaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY