Status
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Gene information
General information
TC007967 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3281290..3282822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007967
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007967 RA
      MAAVIFTGA QLLKQQGGF IGIAYVKTA NYSTESKAY 
NLSALKRGT GGRSSFNGI VATVFGCGG FIGRYVCNR 
LGKNGSQLI LPYRGDPYD VMRLKVCGD LGQVYFHPF 
DLRDEESIE KVCRYSNVV INLIGRDWE TRNFSFDDV 
HVKGARLLA KVAKRSGVE RFIHLSALN AEETPEAVI 
LKGGSKFLA SKWRGEQAV LEEFPEATI FRPADVYGQ 
EDRFLRYYG HIWRRQATY LPLWKKGEE TIKQPVFVS 
DLASGIMAA LKDSDTAGK VYQAVGPKR YYLSELVDW 
FFRVMRKDK DWGYWRYDM RYDPIFQIR VTLTEKLRV 
GFPIGNLHW ERVEREHVT DVVHSEVPT LEDLGVALT 
HMEDQVPWE LKPYTYGLY QGLVDLEEP YTPPAPPTP 
VA
    CDS sequence
    • >TC007967 RA
      atggcggct gtaatattt acaggggcc caattatta 
aaacaacaa gggggtttt atcggaatc gcctacgta 
aaaacagcg aattacagc accgaaagc aaagcgtac 
aacttatca gctttaaaa cggggaact ggtggtaga 
tccagcttt aatggaata gtggcaaca gtttttggg 
tgcgggggc ttcatagga cgttatgtt tgcaataga 
cttggaaaa aacggcagt caattaatc ttgccctac 
agaggcgat ccctatgat gtaatgcgt ttgaaagta 
tgtggggat ttgggccaa gtatatttc cacccgttc 
gatttaaga gatgaagaa tcaatcgaa aaagtctgt 
cgttactca aacgtagtc ataaatttg atcggacga 
gactgggaa acgcgaaac ttcagtttc gatgatgtt 
catgtaaag ggcgcacga cttttagca aaagttgcc 
aaaaggtcg ggggttgag cgatttatt catttgtcg 
gctttgaac gccgaggag acgcctgag gcggtcatc 
ttgaaaggg ggctcgaaa tttttagcg agcaaatgg 
cggggggaa caagccgtc ttggaggag ttccccgag 
gccactatt ttcaggcct gcggacgtc tatggccag 
gaagacaga ttcttgaga tactatggc catatttgg 
agacgccaa gccacctat ttgccccta tggaaaaag 
ggcgaagaa actatcaaa cagccggtg tttgtcagc 
gatttggcg tctggcata atggccgcg ctgaaggat 
tcagacact gctggaaaa gtctaccag gccgtgggt 
ccgaaacgg tactacctc tctgagctt gtggactgg 
ttcttcagg gtgatgcgt aaagacaaa gattggggc 
tactggcgg tacgacatg cgctacgac cccattttc 
caaattaga gtcactttg actgaaaaa ctgcgagtt 
ggtttcccg attggaaac ctacactgg gagcgcgtc 
gaaagggaa catgtcaca gacgtggtc cacagtgaa 
gttcccaca cttgaggat ttaggtgtg gctttgaca 
cacatggag gaccaagtc ccctgggag ctgaaacct 
tacacttat ggtttgtac caaggtctt gttgacctg 
gaggaaccc tacacccca ccggcgcca cctactccc 
gtagcttaa
    mRNA sequence
    • >TC007967 RA
      aattacaaa aatggcggc tgtaatatt tacaggggc 
ccaattatt aaaacaaca agggggttt tatcggaat 
cgcctacgt aaaaacagc gaattacag caccgaaag 
caaagcgta caacttatc agctttaaa acggggaac 
tggtggtag atccagctt taatggaat agtggcaac 
agtttttgg gtgcggggg cttcatagg acgttatgt 
ttgcaatag acttggaaa aaacggcag tcaattaat 
cttgcccta cagaggcga tccctatga tgtaatgcg 
tttgaaagt atgtgggga tttgggcca agtatattt 
ccacccgtt cgatttaag agatgaaga atcaatcga 
aaaagtctg tcgttactc aaacgtagt cataaattt 
gatcggacg agactggga aacgcgaaa cttcagttt 
cgatgatgt tcatgtaaa gggcgcacg acttttagc 
aaaagttgc caaaaggtc gggggttga gcgatttat 
tcatttgtc ggctttgaa cgccgagga gacgcctga 
ggcggtcat cttgaaagg gggctcgaa atttttagc 
gagcaaatg gcgggggga acaagccgt cttggagga 
gttccccga ggccactat tttcaggcc tgcggacgt 
ctatggcca ggaagacag attcttgag atactatgg 
ccatatttg gagacgcca agccaccta tttgcccct 
atggaaaaa gggcgaaga aactatcaa acagccggt 
gtttgtcag cgatttggc gtctggcat aatggccgc 
gctgaagga ttcagacac tgctggaaa agtctacca 
ggccgtggg tccgaaacg gtactacct ctctgagct 
tgtggactg gttcttcag ggtgatgcg taaagacaa 
agattgggg ctactggcg gtacgacat gcgctacga 
ccccatttt ccaaattag agtcacttt gactgaaaa 
actgcgagt tggtttccc gattggaaa cctacactg 
ggagcgcgt cgaaaggga acatgtcac agacgtggt 
ccacagtga agttcccac acttgagga tttaggtgt 
ggctttgac acacatgga ggaccaagt cccctggga 
gctgaaacc ttacactta tggtttgta ccaaggtct 
tgttgacct ggaggaacc ctacacccc accggcgcc 
acctactcc cgtagctta aattattgt ataaaaagg 
aaactaaat tcaaacaca cgtt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY