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Gene information
General information
TC007967 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3281290..3282822
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007967
    iBeetle screen iB_09030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007967 RA
      MAAVIFTGA QLLKQQGGF IGIAYVKTA NYSTESKAY 
      NLSALKRGT GGRSSFNGI VATVFGCGG FIGRYVCNR 
      LGKNGSQLI LPYRGDPYD VMRLKVCGD LGQVYFHPF 
      DLRDEESIE KVCRYSNVV INLIGRDWE TRNFSFDDV 
      HVKGARLLA KVAKRSGVE RFIHLSALN AEETPEAVI 
      LKGGSKFLA SKWRGEQAV LEEFPEATI FRPADVYGQ 
      EDRFLRYYG HIWRRQATY LPLWKKGEE TIKQPVFVS 
      DLASGIMAA LKDSDTAGK VYQAVGPKR YYLSELVDW 
      FFRVMRKDK DWGYWRYDM RYDPIFQIR VTLTEKLRV 
      GFPIGNLHW ERVEREHVT DVVHSEVPT LEDLGVALT 
      HMEDQVPWE LKPYTYGLY QGLVDLEEP YTPPAPPTP 
      VA
    CDS sequence
    • >TC007967 RA
      atggcggct gtaatattt acaggggcc caattatta 
      aaacaacaa gggggtttt atcggaatc gcctacgta 
      aaaacagcg aattacagc accgaaagc aaagcgtac 
      aacttatca gctttaaaa cggggaact ggtggtaga 
      tccagcttt aatggaata gtggcaaca gtttttggg 
      tgcgggggc ttcatagga cgttatgtt tgcaataga 
      cttggaaaa aacggcagt caattaatc ttgccctac 
      agaggcgat ccctatgat gtaatgcgt ttgaaagta 
      tgtggggat ttgggccaa gtatatttc cacccgttc 
      gatttaaga gatgaagaa tcaatcgaa aaagtctgt 
      cgttactca aacgtagtc ataaatttg atcggacga 
      gactgggaa acgcgaaac ttcagtttc gatgatgtt 
      catgtaaag ggcgcacga cttttagca aaagttgcc 
      aaaaggtcg ggggttgag cgatttatt catttgtcg 
      gctttgaac gccgaggag acgcctgag gcggtcatc 
      ttgaaaggg ggctcgaaa tttttagcg agcaaatgg 
      cggggggaa caagccgtc ttggaggag ttccccgag 
      gccactatt ttcaggcct gcggacgtc tatggccag 
      gaagacaga ttcttgaga tactatggc catatttgg 
      agacgccaa gccacctat ttgccccta tggaaaaag 
      ggcgaagaa actatcaaa cagccggtg tttgtcagc 
      gatttggcg tctggcata atggccgcg ctgaaggat 
      tcagacact gctggaaaa gtctaccag gccgtgggt 
      ccgaaacgg tactacctc tctgagctt gtggactgg 
      ttcttcagg gtgatgcgt aaagacaaa gattggggc 
      tactggcgg tacgacatg cgctacgac cccattttc 
      caaattaga gtcactttg actgaaaaa ctgcgagtt 
      ggtttcccg attggaaac ctacactgg gagcgcgtc 
      gaaagggaa catgtcaca gacgtggtc cacagtgaa 
      gttcccaca cttgaggat ttaggtgtg gctttgaca 
      cacatggag gaccaagtc ccctgggag ctgaaacct 
      tacacttat ggtttgtac caaggtctt gttgacctg 
      gaggaaccc tacacccca ccggcgcca cctactccc 
      gtagcttaa
    mRNA sequence
    • >TC007967 RA
      aattacaaa aatggcggc tgtaatatt tacaggggc 
      ccaattatt aaaacaaca agggggttt tatcggaat 
      cgcctacgt aaaaacagc gaattacag caccgaaag 
      caaagcgta caacttatc agctttaaa acggggaac 
      tggtggtag atccagctt taatggaat agtggcaac 
      agtttttgg gtgcggggg cttcatagg acgttatgt 
      ttgcaatag acttggaaa aaacggcag tcaattaat 
      cttgcccta cagaggcga tccctatga tgtaatgcg 
      tttgaaagt atgtgggga tttgggcca agtatattt 
      ccacccgtt cgatttaag agatgaaga atcaatcga 
      aaaagtctg tcgttactc aaacgtagt cataaattt 
      gatcggacg agactggga aacgcgaaa cttcagttt 
      cgatgatgt tcatgtaaa gggcgcacg acttttagc 
      aaaagttgc caaaaggtc gggggttga gcgatttat 
      tcatttgtc ggctttgaa cgccgagga gacgcctga 
      ggcggtcat cttgaaagg gggctcgaa atttttagc 
      gagcaaatg gcgggggga acaagccgt cttggagga 
      gttccccga ggccactat tttcaggcc tgcggacgt 
      ctatggcca ggaagacag attcttgag atactatgg 
      ccatatttg gagacgcca agccaccta tttgcccct 
      atggaaaaa gggcgaaga aactatcaa acagccggt 
      gtttgtcag cgatttggc gtctggcat aatggccgc 
      gctgaagga ttcagacac tgctggaaa agtctacca 
      ggccgtggg tccgaaacg gtactacct ctctgagct 
      tgtggactg gttcttcag ggtgatgcg taaagacaa 
      agattgggg ctactggcg gtacgacat gcgctacga 
      ccccatttt ccaaattag agtcacttt gactgaaaa 
      actgcgagt tggtttccc gattggaaa cctacactg 
      ggagcgcgt cgaaaggga acatgtcac agacgtggt 
      ccacagtga agttcccac acttgagga tttaggtgt 
      ggctttgac acacatgga ggaccaagt cccctggga 
      gctgaaacc ttacactta tggtttgta ccaaggtct 
      tgttgacct ggaggaacc ctacacccc accggcgcc 
      acctactcc cgtagctta aattattgt ataaaaagg 
      aaactaaat tcaaacaca cgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY