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Gene information
General information
TC031341
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016089
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1496065..1500012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031341
    iBeetle screen iB_09029

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031341 RA
      MFWKTKIGF CFLLLTPIL CDSNFTNIY DEFFEVVVN 
      RKELSARIK TELGDSNEA IAAYIKMLD AWSKFPSGI 
      MEGNLMDLG SFDECYAIE YNNIYGKYC LGNLHFSFQ 
      MENFFKTFG ISDAKPRPR ILLHPQPRL MGLGDEYTG 
      IHFAACVPS TLPASEIPG LFNYTEEFC YSKATEPEL 
      SAGAIVTIT ILAIFLCIV LVSTSYDVA LNYFKKEPY 
      HELLIAFSF LHNGRKLFR SSKNSEQLL CLNGIRALS 
      MMWVILGHE YSNSFNAPI SNFFDLKTW QDDPANMFI 
      MGATVSVDT FFTAGGLVT VYTFLKSMD KGANFNVLL 
      FYVHRYLRL TPAYFIMGL IHLFLLNHF GTGPLWKVV 
      DISLVDTCE TGWWSSLLY ITNYVQKGT CLPQAWYLQ 
      VDMQLFVLS PLVLIPLFR WPKIGLGAL GFLTIMGCV 
      SPFVIGYVK HLGGGMINN DDTNEFMNY YYAATYARF 
      GPYVIGMLA GYLLYKIKT NKIKVNLKM WQVITLWLV 
      LLTGLVACV WAGFPLNAS KDDRWGNSL FLAFNRPAW 
      AVAVVGVVF LCVAGYGGP IDKFLSWSV FQFLTKVSY 
      SMYLVHFAV ITVRYALLR NNIKFSNLA MMHAFWGDF 
      MFTLGLSMI LCSTFESPI IILEKYLFH RGPKTKSVK 
      Q
    CDS sequence
    • >TC031341 RA
      atgttttgg aagaccaaa attggtttt tgctttctg 
      ttgctaact cctatactt tgtgatagt aactttacc 
      aacatttac gatgaattt tttgaagtg gtggtgaat 
      agaaaagag ttaagtgcc cgaatcaaa actgaattg 
      ggtgactca aatgaagcc attgctgca tatattaaa 
      atgttggat gcttggtct aaatttcca agtggtata 
      atggagggc aatttaatg gatttggga agttttgac 
      gaatgttac gccatcgaa tataacaac atttatgga 
      aagtattgc ttgggaaat ttacatttc agtttccaa 
      atggaaaat tttttcaaa acttttgga attagcgat 
      gcaaaacct cgtccgaga attttgctg cacccacaa 
      cccagatta atgggttta ggtgacgag tatacagga 
      attcatttt gctgcatgt gtaccaagt accttgcca 
      gcatcggaa attcctgga cttttcaac tatacggaa 
      gaattttgt tattctaaa gcaacagaa ccggaacta 
      tcagcgggg gcgattgtg accataaca attttagcc 
      atttttctg tgtatagtg ctagtttca acaagctat 
      gatgttgcc ctaaactac ttcaaaaaa gaaccctat 
      cacgaattg ttgattgca ttttcgttt cttcataac 
      ggacgaaaa ctcttccgt tcgagcaaa aattcagag 
      cagttgctc tgtctgaac ggaattaga gcattgagc 
      atgatgtgg gttatcctg ggacacgaa tactcaaat 
      tcttttaat gctcccatc tccaatttc tttgattta 
      aaaacctgg caagacgat cccgctaat atgtttata 
      atgggagcc acagtctcc gttgataca tttttcaca 
      gctggaggt cttgtaaca gtttacaca tttctgaaa 
      tcaatggac aagggagca aatttcaat gtgttactt 
      ttctacgtc catagatat cttcgtcta acccctgcc 
      tattttata atgggttta atccatctc ttcctctta 
      aaccacttc ggaactgga cctttgtgg aaagttgta 
      gatatttcg ttagttgat acgtgcgag acaggctgg 
      tggagtagt ctgttgtac attacaaat tatgtacaa 
      aaagggacc tgtttgcct caagcttgg tacctccaa 
      gtggatatg caattattt gtcttgtca ccacttgtt 
      ctcataccg ttgttcagg tggcccaaa attggttta 
      ggtgcttta ggtttctta acaatcatg ggatgtgta 
      tcgccgttt gtgattggt tacgtcaag cacttaggg 
      ggcggaatg ataaataat gatgacact aacgaattt 
      atgaattat tattacgct gcaacgtat gccagattc 
      ggaccatat gtaattgga atgcttgct ggttactta 
      ttgtacaaa attaaaacc aacaagatc aaagttaat 
      ttgaaaatg tggcaagtt atcacgctt tggttggtg 
      cttctgacc ggtttagtg gcctgtgtg tgggccggt 
      tttccacta aatgcctcc aaagatgat cgatgggga 
      aattccctg tttttagct tttaatcga ccagcttgg 
      gctgtggca gttgtagga gtagttttc ttgtgtgtg 
      gctggttat gggggacct attgataag ttcctttct 
      tggtcagtg ttccaattt ttgacgaaa gtttcgtat 
      tctatgtac ttggtacat tttgctgtt atcacagtc 
      agatatgca ctcttgagg aataatata aaattctcc 
      aatcttgct atgatgcat gccttctgg ggagatttt 
      atgtttacc ttgggcttg tctatgatc ttgtgctca 
      actttcgaa tctccaatt attattttg gaaaaatat 
      cttttccat agaggcccc aaaacaaaa agtgtaaag 
      caataa
    mRNA sequence
    • >TC031341 RA
      gttctaaag caacatgtt ttggaagac caaaattgg 
      tttttgctt tctgttgct aactcctat actttgtga 
      tagtaactt taccaacat ttacgatga attttttga 
      agtggtggt gaatagaaa agagttaag tgcccgaat 
      caaaactga attgggtga ctcaaatga agccattgc 
      tgcatatat taaaatgtt ggatgcttg gtctaaatt 
      tccaagtgg tataatgga gggcaattt aatggattt 
      gggaagttt tgacgaatg ttacgccat cgaatataa 
      caacattta tggaaagta ttgcttggg aaatttaca 
      tttcagttt ccaaatgga aaatttttt caaaacttt 
      tggaattag cgatgcaaa acctcgtcc gagaatttt 
      gctgcaccc acaacccag attaatggg tttaggtga 
      cgagtatac aggaattca ttttgctgc atgtgtacc 
      aagtacctt gccagcatc ggaaattcc tggactttt 
      caactatac ggaagaatt ttgttattc taaagcaac 
      agaaccgga actatcagc gggggcgat tgtgaccat 
      aacaatttt agccatttt tctgtgtat agtgctagt 
      ttcaacaag ctatgatgt tgccctaaa ctacttcaa 
      aaaagaacc ctatcacga attgttgat tgcattttc 
      gtttcttca taacggacg aaaactctt ccgttcgag 
      caaaaattc agagcagtt gctctgtct gaacggaat 
      tagagcatt gagcatgat gtgggttat cctgggaca 
      cgaatactc aaattcttt taatgctcc catctccaa 
      tttctttga tttaaaaac ctggcaaga cgatcccgc 
      taatatgtt tataatggg agccacagt ctccgttga 
      tacattttt cacagctgg aggtcttgt aacagttta 
      cacatttct gaaatcaat ggacaaggg agcaaattt 
      caatgtgtt acttttcta cgtccatag atatcttcg 
      tctaacccc tgcctattt tataatggg tttaatcca 
      tctcttcct cttaaacca cttcggaac tggaccttt 
      gtggaaagt tgtagatat ttcgttagt tgatacgtg 
      cgagacagg ctggtggag tagtctgtt gtacattac 
      aaattatgt acaaaaagg gacctgttt gcctcaagc 
      ttggtacct ccaagtgga tatgcaatt atttgtctt 
      gtcaccact tgttctcat accgttgtt caggtggcc 
      caaaattgg tttaggtgc tttaggttt cttaacaat 
      catgggatg tgtatcgcc gtttgtgat tggttacgt 
      caagcactt agggggcgg aatgataaa taatgatga 
      cactaacga atttatgaa ttattatta cgctgcaac 
      gtatgccag attcggacc atatgtaat tggaatgct 
      tgctggtta cttattgta caaaattaa aaccaacaa 
      gatcaaagt taatttgaa aatgtggca agttatcac 
      gctttggtt ggtgcttct gaccggttt agtggcctg 
      tgtgtgggc cggttttcc actaaatgc ctccaaaga 
      tgatcgatg gggaaattc cctgttttt agcttttaa 
      tcgaccagc ttgggctgt ggcagttgt aggagtagt 
      tttcttgtg tgtggctgg ttatggggg acctattga 
      taagttcct ttcttggtc agtgttcca atttttgac 
      gaaagtttc gtattctat gtacttggt acattttgc 
      tgttatcac agtcagata tgcactctt gaggaataa 
      tataaaatt ctccaatct tgctatgat gcatgcctt 
      ctggggaga ttttatgtt taccttggg cttgtctat 
      gatcttgtg ctcaacttt cgaatctcc aattattat 
      tttggaaaa atatctttt ccatagagg ccccaaaac 
      aaaaagtgt aaagcaata actaattat gtataaaaa 
      tataagtaa tatactcgt atttctgct gtacataca 
      taaattatt agaataaat ttgttataa aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY