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Gene information
General information
TC002396 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 31278294..31283602
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002396
    iBeetle screen iB_09027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002396 RA
      MNKFGRCLV TLNRQFRTS TQTRTRKKP FSLRNVTIV 
      GSLAFGGGI AASKTDEKY LSFVGGIPR FCRSIKTGL 
      LISLDYYFS MLGLTESDP NYELMMSRI HQRAADRIL 
      AACLTNGGP YIKMGQGLV SMSHILPKE YTKTLKALQ 
      DKCLPRHPN ELVKLFQED FQKTPDEIF ENFDPNPIA 
      AASLAQVYK AKTQTGEEV AVKVQYIDL QKRFLSDVA 
      TIKLLLKVV GMMHPNFNF GWVLEEVAD TLKQELDFV 
      NEGKNAEKC AKDLEKFDF VHVPKIYWD LTSTRVLVM 
      EYIEGCKIS DVKFLKEKK FSLKDINNK LFEIFGHQI 
      FQTGFVHGD PHAGNILVR RVEGKTQLV LLDHGLYQR 
      LKPQEMVAL SHMWKAIVL QDHAQMKFY SKQLGVEDY 
      VVLPKF
    CDS sequence
    • >TC002396 RA
      atgaacaag ttcggccgc tgccttgta acattaaac 
      cgccaattc cgaacttcc acacaaact cggacccga 
      aaaaagcct ttttctctt cgaaatgtc acaattgtc 
      ggaagtctg gcctttgga ggtggcatt gctgcttcg 
      aaaactgac gaaaaatac ctcagtttc gtcggggga 
      atcccccga ttctgtcgc tcaataaaa actggtctc 
      ctcatctcc ctggactat tacttctcc atgctcggt 
      ttaaccgaa tctgaccca aattacgaa ctaatgatg 
      agccgcatc caccaaaga gcagccgat cgcatcctc 
      gcagcatgt ctcacaaac ggggggcct tacataaaa 
      atgggccaa ggtctagtc tccatgtcc cacatcctc 
      cccaaagaa tacaccaaa acattgaaa gccctccaa 
      gacaagtgt ctcccacgc caccccaac gaactagtc 
      aaactattc caagaagat ttccaaaaa acccccgat 
      gaaattttc gaaaatttc gacccgaat ccaatcgca 
      gcagccagt ttggctcaa gtctacaaa gccaagacc 
      caaactggc gaagaagtt gccgttaaa gtgcaatat 
      atcgatttg caaaaacgc tttttaagc gacgttgcc 
      acaattaaa ttattattg aaagtggtg gggatgatg 
      cacccgaat tttaacttc ggttgggtg cttgaggag 
      gttgctgat acgttgaaa caggagttg gattttgtt 
      aatgagggg aaaaatgcg gaaaagtgt gccaaagat 
      ttggaaaag tttgatttt gttcatgtg cctaaaatc 
      tattgggat ctcacaagc acgagggtt ttggtgatg 
      gaatatatc gagggttgt aagattagc gatgttaag 
      tttttgaag gaaaaaaag ttttcgttg aaagatatc 
      aataataag ttgtttgaa atttttggg catcagatt 
      tttcaaaca gggttcgtt catggggac ccgcatgct 
      ggaaacatt ctggtacga cgagttgag ggcaaaacc 
      caactagtc ctcctggac catggctta taccaaaga 
      ctaaaaccg caagaaatg gtcgctttg agccacatg 
      tggaaagcc atagtatta caagaccac gcacaaatg 
      aaattctat tcgaaacaa ttaggagtt gaagattat 
      gtggttttg ccgaaattt taa
    mRNA sequence
    • >TC002396 RA
      cgtgattat tttcagttt ttttttggc aaaggtgac 
      ttgattaat tgatgacat accttaagt ttgcggtta 
      cgtcgaaaa caacggtag atggggtcg aaaaacaca 
      aagtctaac tggtctcaa caagcacag cagcattca 
      gcccagtgc aggcgctga ataaaccat ttttcaggg 
      gtttgcatg ggtctgggt cgggggtag tgcgggggt 
      acgtcccct ctcactcta acacgtttg gttcgacat 
      cagtctgtc tcacttgtc ggatgttgc gcactaacg 
      ttgtgtttt gatcgcagt ggcttgtga ttttatttg 
      ttttttggt aaattagac aattagagt catccgaag 
      actagatcg gataaatga tttaataaa aatggcaaa 
      aacttgttg gtttttaca ataaatggg tctgacgac 
      acggcagtt tgtttagtt ttatgactt tggtaataa 
      aatcgtaaa atgtacttt agtaaaggc agcgttatg 
      tattcgtct acattttta aaattttct ttcaggtat 
      agtttaaaa aatgaacaa gttcggccg ctgccttgt 
      aacattaaa ccgccaatt ccgaacttc cacacaaac 
      tcggacccg aaaaaagcc tttttctct tcgaaatgt 
      cacaattgt cggaagtct ggcctttgg aggtggcat 
      tgctgcttc gaaaactga cgaaaaata cctcagttt 
      cgtcggggg aatcccccg attctgtcg ctcaataaa 
      aactggtct cctcatctc cctggacta ttacttctc 
      catgctcgg tttaaccga atctgaccc aaattacga 
      actaatgat gagccgcat ccaccaaag agcagccga 
      tcgcatcct cgcagcatg tctcacaaa cggggggcc 
      ttacataaa aatgggcca aggtctagt ctccatgtc 
      ccacatcct ccccaaaga atacaccaa aacattgaa 
      agccctcca agacaagtg tctcccacg ccaccccaa 
      cgaactagt caaactatt ccaagaaga tttccaaaa 
      aacccccga tgaaatttt cgaaaattt cgacccgaa 
      tccaatcgc agcagccag tttggctca agtctacaa 
      agccaagac ccaaactgg cgaagaagt tgccgttaa 
      agtgcaata tatcgattt gcaaaaacg ctttttaag 
      cgacgttgc cacaattaa attattatt gaaagtggt 
      ggggatgat gcacccgaa ttttaactt cggttgggt 
      gcttgagga ggttgctga tacgttgaa acaggagtt 
      ggattttgt taatgaggg gaaaaatgc ggaaaagtg 
      tgccaaaga tttggaaaa gtttgattt tgttcatgt 
      gcctaaaat ctattggga tctcacaag cacgagggt 
      tttggtgat ggaatatat cgagggttg taagattag 
      cgatgttaa gtttttgaa ggaaaaaaa gttttcgtt 
      gaaagatat caataataa gttgtttga aatttttgg 
      gcatcagat ttttcaaac agggttcgt tcatgggga 
      cccgcatgc tggaaacat tctggtacg acgagttga 
      gggcaaaac ccaactagt cctcctgga ccatggctt 
      ataccaaag actaaaacc gcaagaaat ggtcgcttt 
      gagccacat gtggaaagc catagtatt acaagacca 
      cgcacaaat gaaattcta ttcgaaaca attaggagt 
      tgaagatta tgtggtttt gccgaaatt ttaacacaa 
      gcgcctttg cgacgccac aaattcaaa ttacacata 
      agattgact gacgaagat tgaaacaca tgactgaaa 
      ttgcaagga cacggtttg atgctataa tgaaggctt 
      tgaggttga tgcccactt ctctcctac ttgttatca 
      ggtaaaaaa atatagtta tcaaataaa aaaatatag 
      ttatcagat aaaaaaata tagtttgag tcactcatt 
      cgctacgtt tgtattttt tttaaataa tatataatt 
      ttttttcta gaaatttga acacaatcc gagctatag 
      catacgacc atggcagtc ccattgata gatacactg 
      ttttggcaa gggctgcta ctaaaacag cccttaagg 
      acgaaagtt cgtcgaaaa aattcctca aagctccgt 
      tgaaaatct acttcgaag ttgttttat tgtaagtcg 
      tttaaaaac atcaaattt atgtgaatt aattcgttt 
      gtttcagct taaataggc tcaatttgt ggtttagaa 
      atgtgatat ataatttat tggttagtt taggtttgc 
      ttccagatt tgaagcaaa tcattgttg ttcctcagg 
      tttaaaata aatttattt gtgtttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY