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Gene information
General information
TC034627 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 25193201..25213610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034627
    iBeetle screen iB_09022

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034627 RA
      MFATLLVIL TIFAIIYTA CSSDLNDGV ANEYVLEEL 
      NDNFGFLAI VCCIGLGLS VIALFCFRY FVNIFIWTM 
      LIVTVLLTC ALAGFVWYI SAQPVSHNQ ISFKIAAII 
      LTAFSLITA TCILVWNEQ IKLLIKFYE EAIKIVFAM 
      PQIFLVSLL TSISVLGLI VTAICGTPL CQSSVRGAT 
      LVDSNFLSA VAKSSAGTF VFSTMQLLV FNITAIIGF 
      FMIHSVQSV LIGAVFALV CAHCIFVVF QIAVDTLFI 
      CYCEDYCLN NGINEPFYA TAELRQKKT FHDKMYIKL 
      QKMVPSDLF INLGLCQKR KLTDVPMLI IFAICVAIL 
      MIIAMIYTI SSSDINRGT NGHVLRVLI NNSGSLAIG 
      CCIGLGLSL IALFCFRYC VNVFIWTVL IVTVVLMFT 
      NVGFSGYFW YCSGELYLN YEITKLITF IISTFVFLI 
      STILVLVLR EQIKLIIEF YKEAMKILF AMPLIFLVP 
      LLTFISISG IFALFCYIT FPRIPMNDA GEFYHVIWT 
      SCIFGVVRI TVDTVFICY CEDYRLNNG TNEPFYATV 
      ELHQKLKKV QDFTKGIEE KACVL
    CDS sequence
    • >TC034627 RA
      atgtttgca acacttctg gttattttg acgatcttt 
      gcaatcata tacactgca tgttcttcg gatttgaac 
      gacggagtt gcgaatgaa tatgtcttg gaagagtta 
      aacgacaac tttggattt ctggcaata gtttgctgc 
      ataggactt ggtctttct gttatcgca ttattttgc 
      tttcgttat tttgtgaac atatttatt tggactatg 
      ttgatagtt actgtctta ttgacgtgt gcgctcgca 
      ggatttgtt tggtatatt tcggctcag ccggtttca 
      cacaaccaa atcagtttt aagatagcc gctatcatt 
      ttaacagct ttttcgctt atcactgca acatgtatt 
      ctagtgtgg aatgaacaa attaaactt ctaatcaaa 
      ttttacgag gaagctatc aaaatagtg ttcgcgatg 
      cctcaaatt tttctggta tcgctcttg acatccatc 
      tccgttttg ggtttaatc gtcacagct atttgtgga 
      acaccctta tgccaatcg agtgttcgt ggtgcgact 
      ttagtcgat agcaatttt ctgagtgct gtggcgaag 
      agttcggcg ggaactttc gtgttttcc acgatgcaa 
      ttgcttgtg tttaatatt actgcgatt attggtttt 
      tttatgata cattcagtc caatcagtc ttaattgga 
      gctgtattt gcattggtg tgcgctcac tgtatcttt 
      gttgttttc caaattgct gttgatact ttatttatc 
      tgttactgt gaggattac tgccttaac aatggtata 
      aatgaacca ttttatgca actgcggag cttcggcaa 
      aagaagaca ttccacgac aaaatgtac attaaactg 
      caaaaaatg gtgccttcg gaccttttc atcaatctt 
      ggcctctgc caaaaaaga aaattaacc gacgttccc 
      atgcttata atatttgcg atatgtgtt gcaattttg 
      atgatcatt gctatgatc tacactata tcttcttcg 
      gatataaac agaggtacg aatggacat gtcttgaga 
      gtattaatt aataactcg ggttctcta gcaataggt 
      tgctgcatt ggacttggt ctttctctt atcgcatta 
      ttttgcttt cgctattgt gtgaatgta tttatttgg 
      actgtgttg atagttaca gtcgtacta atgtttacg 
      aacgtagga ttttctgga tacttttgg tattgctcg 
      ggcgaactg tatttaaac tacgaaatc actaagctg 
      ataacgttc attatttca acatttgtt tttcttatt 
      agtacaata ttggttcta gtgttgaga gagcaaatt 
      aaacttata atcgaattt tacaaagaa gctatgaaa 
      atactattt gctatgcct ctaattttt ctggtacct 
      ctcttgaca ttcatctcc atctcgggg atttttgcg 
      ttattctgt tacataact tttccaagg attccaatg 
      aacgacgct ggagaattt tatcacgtt atatggact 
      tcctgtatc tttggtgta gtcaggatt actgtagac 
      acagtattc atctgttac tgtgaagat taccgcctt 
      aacaatggt acaaatgaa ccattttat gcaactgtg 
      gagcttcac caaaagctg aagaaagtg caagacttc 
      acaaagggt attgaggaa aaggcatgt gttttataa
    mRNA sequence
    • >TC034627 RA
      aagaaaatt aaccgactt atcctacct tttgatgtt 
      tgcaacact tctggttat tttgacgat ctttgcaat 
      catatacac tgcatgttc ttcggattt gaacgacgg 
      agttgcgaa tgaatatgt cttggaaga gttaaacga 
      caactttgg atttctggc aatagtttg ctgcatagg 
      acttggtct ttctgttat cgcattatt ttgctttcg 
      ttattttgt gaacatatt tatttggac tatgttgat 
      agttactgt cttattgac gtgtgcgct cgcaggatt 
      tgtttggta tatttcggc tcagccggt ttcacacaa 
      ccaaatcag ttttaagat agccgctat cattttaac 
      agctttttc gcttatcac tgcaacatg tattctagt 
      gtggaatga acaaattaa acttctaat caaatttta 
      cgaggaagc tatcaaaat agtgttcgc gatgcctca 
      aatttttct ggtatcgct cttgacatc catctccgt 
      tttgggttt aatcgtcac agctatttg tggaacacc 
      cttatgcca atcgagtgt tcgtggtgc gactttagt 
      cgatagcaa ttttctgag tgctgtggc gaagagttc 
      ggcgggaac tttcgtgtt ttccacgat gcaattgct 
      tgtgtttaa tattactgc gattattgg tttttttat 
      gatacattc agtccaatc agtcttaat tggagctgt 
      atttgcatt ggtgtgcgc tcactgtat ctttgttgt 
      tttccaaat tgctgttga tactttatt tatctgtta 
      ctgtgagga ttactgcct taacaatgg tataaatga 
      accatttta tgcaactgc ggagcttcg gcaaaagaa 
      gacattcca cgacaaaat gtacattaa actgcaaaa 
      aatggtgcc ttcggacct tttcatcaa tcttggcct 
      ctgccaaaa aagaaaatt aaccgacgt tcccatgct 
      tataatatt tgcgatatg tgttgcaat tttgatgat 
      cattgctat gatctacac tatatcttc ttcggatat 
      aaacagagg tacgaatgg acatgtctt gagagtatt 
      aattaataa ctcgggttc tctagcaat aggttgctg 
      cattggact tggtctttc tcttatcgc attattttg 
      ctttcgcta ttgtgtgaa tgtatttat ttggactgt 
      gttgatagt tacagtcgt actaatgtt tacgaacgt 
      aggattttc tggatactt ttggtattg ctcgggcga 
      actgtattt aaactacga aatcactaa gctgataac 
      gttcattat ttcaacatt tgtttttct tattagtac 
      aatattggt tctagtgtt gagagagca aattaaact 
      tataatcga attttacaa agaagctat gaaaatact 
      atttgctat gcctctaat ttttctggt acctctctt 
      gacattcat ctccatctc ggggatttt tgcgttatt 
      ctgttacat aacttttcc aaggattcc aatgaacga 
      cgctggaga attttatca cgttatatg gacttcctg 
      tatctttgg tgtagtcag gattactgt agacacagt 
      attcatctg ttactgtga agattaccg ccttaacaa 
      tggtacaaa tgaaccatt ttatgcaac tgtggagct 
      tcaccaaaa gctgaagaa agtgcaaga cttcacaaa 
      gggtattga ggaaaaggc atgtgtttt ataaattta 
      ggaaattaa ttgtgaaaa ttgtatttt ttgtaattg 
      taaaagtcg tggattatt tgaccacgt ttttagttt 
      aacttttct aacaatttt atcaattgt tcttctcac 
      aaaaatata ttacacctt gcttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY