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Gene information
General information
TC010332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24961575..24967920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010332
    iBeetle screen iB_09021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010332 RA
      MALTLLGKR ILSRRPQLL NSIRKNGYI VLVPEIGED 
      LPEKNPLLQ NDGLPEFSN ITIENCMAA IAKQTLDLE 
      AGVKEIETS LSETGTTDI FHDVFQVLE EIVAPLDLT 
      WGLSKTLYL GNSSLMPTK SYLAIHDRA KRARAAKFN 
      SPVIYESVK RELQGEAPH SEEETRILN KFALEGKLN 
      GLELTDSQK VQLKQHINK LTAERLQFK QKVDICTKQ 
      FSHTIRDSS IVRDFPLDL LKTTASNPL KPLDGPWKV 
      TLDPHVYLP IMEHCPERE IRWNVWQAL VSRGSGYGD 
      RDLSTGVHL EEIRFARRD LAKLLGFET YADLSMETK 
      MAENVGNVN SMLHTLLEK AKPAQETEL ASLYEFALE 
      RGFKGGRIE LWDVPYWRK KQLKTLFNY NEEEFKNYF 
      PLPTVLTGL FELCERLFN VVIKQRNDI STWHKDVKF 
      YDIFEPQTS APIAGFYLD PYARSDEKI TIQQSNGWM 
      VGIQSQSKI TATKPLAAL IFNFEPPSG NKQSHLTFK 
      EVKHLFHKF GLALQHLLT RTTYSEVSG SSNIEWDAV 
      EVSGHVLAH WLYNKEIMQ NISCHCHSR EKLPESMFD 
      CLMEAHKHL AGIDLSREL YLAALDLEL HHSTEYWLE 
      IVKKLWPQF RCFPLDKLD SHPCSFTQI FTEEWAAAY 
      YSHVWSRMI AADVYSAFH EVQGDDQKI RDVGKRYRE 
      TFLALGGSC HPSKVFRKF RGRDPSPNA LLRGFKGGR 
      IELWDVPYW RKKQLKTLF DYNEEEFKN YFPLPTVLT 
      GLFELCERL FNVVIKQRN DISTWHKDV KFYDIFEPQ 
      TSAPIAGFY LDPYARSDE KITIQQSNG WMVGIQSQS 
      KITATKPLA ALIFNFEPP SGNKQSHLT FKEVKHLFH 
      KFGLALQHL LTRTTYSEV SGSSNIEWD AVEVSGHVL 
      AHWLYNKEI MQNISCHCH SREKLPESM FDCLMEAHK 
      HLAGIDLSR ELYLAALDL ELHHSTEYW LEIVKKLWP 
      QFRCFPLDK LDSHPCSFT QIFTEEWAA AYYSHVWSR 
      MIAADVYSA FHEVQGDDQ KIRDVVAIL VKFLGSLED 
      GIRRRMRCL GR
    CDS sequence
    • >TC010332 RA
      atggcatta acactgttg ggaaagcgt atcttgtca 
      agacggcca caacttctc aactccatt agaaaaaat 
      ggctacatc gttttggtg ccagaaatc ggtgaagac 
      ttacccgag aaaaacccc ctcctccag aacgacggc 
      ttgcccgaa ttcagcaac atcaccatt gaaaattgc 
      atggcagca atcgccaaa caaacgctc gacttggag 
      gccggtgtg aaagaaatc gaaacatcg ttaagcgaa 
      accggaacc actgacatt ttccacgac gtgtttcaa 
      gtcctggag gagatcgtg gcgcccttg gacctaacc 
      tgggggcta tcaaaaacg ctctactta ggtaacagt 
      agtctaatg cccaccaaa agctacttg gccatccac 
      gaccgcgca aaacgggcc cgtgccgcg aaattcaac 
      agtccggtg atttacgag tcggttaaa agggaactg 
      cagggcgaa gccccccac agtgaggag gaaactagg 
      atattgaac aaattcgct ttggagggc aaactaaac 
      ggtctggaa ctcaccgac tcgcagaaa gtccagctc 
      aagcaacac attaacaaa ctcacagct gagcggttg 
      cagttcaag caaaaagtc gacatttgt accaaacaa 
      ttttctcac accatccgg gactcgtcg attgtgcgc 
      gattttccg ctagatttg ctaaaaacg acggcttca 
      aatccgtta aaaccgctc gatggcccc tggaaggtg 
      actctggac ccccatgtt tacttgccg attatggaa 
      cattgccct gaacgtgaa atacggtgg aatgtctgg 
      caggcgttg gtaagtcgg ggctcgggt tatggggat 
      cgtgatttg tcaacgggg gtccatttg gaggaaatt 
      aggtttgcg agacgcgat ttagctaaa ttgttaggt 
      tttgaaacg tatgctgat ctttcaatg gagacgaaa 
      atggcagag aatgttggt aatgtgaac agtatgttg 
      catactctc cttgagaag gctaagccg gcacaagag 
      actgaactg gccagtttg tacgaattt gccctcgaa 
      agggggttc aaggggggt aggatcgaa ctgtgggac 
      gttccgtat tggaggaag aaacagctc aaaacactg 
      ttcaattac aacgaggag gagttcaag aattatttt 
      cccctaccc acggtttta accggtttg tttgagctg 
      tgtgagaga ttgttcaat gtggtgatc aaacaacga 
      aacgacata tctacctgg cacaaagac gtcaaattt 
      tacgatatt ttcgagccc cagacaagc gcccccatt 
      gccgggttt tacctcgac ccgtacgcg cgctcagac 
      gagaaaata accatccag caaagcaac ggttggatg 
      gttggcatc caaagtcag agcaaaatc accgcgact 
      aaaccatta gcagccctc atcttcaac tttgagccc 
      ccttcaggc aacaaacaa tctcacttg acgttcaaa 
      gaagtcaaa cacttgttc cacaagttt ggcttagcc 
      ctccagcat ttactcact cgaaccacg tactcagaa 
      gtctcaggg tcctcgaat attgagtgg gatgcggtc 
      gaagtcagt ggccacgtc ctggcccac tggctgtac 
      aataaggag ataatgcag aacatttct tgccattgt 
      cacagtcgg gagaaactc cccgaaagt atgtttgat 
      tgcttgatg gaggcgcac aagcacttg gccgggatt 
      gacttatcg agagagctg tatttggcg gcgcttgat 
      ttagaactg caccattcg accgagtat tggctggaa 
      atagtgaag aagttgtgg ccgcagttc cggtgcttc 
      cctcttgat aaactcgac tcgcatcct tgttccttc 
      acgcagatt tttacagag gagtgggct gcggcttat 
      tactcgcac gtctggtcg aggatgatt gccgctgat 
      gtgtacagt gcgtttcat gaagtgcag ggagatgac 
      caaaaaatt agagatgta gggaagaga tatagggaa 
      acgtttttg gccttggga ggtagttgc catcctagt 
      aaagttttt aggaagttt agaggacgg gatccgtcg 
      ccgaacgcg ttgcttaga gggttcaag gggggtaga 
      atcgaactg tgggacgtc ccgtattgg aggaagaaa 
      cagctcaaa acactgttc gattacaac gaggaggag 
      ttcaagaat tattttccc ttgcccacg gttttaacc 
      ggcttgttt gagctgtgt gagagattg ttcaatgtg 
      gtgatcaaa caacgaaac gacatatcc acctggcac 
      aaagacgtc aaattctac gatattttc gagccccag 
      acaagcgcc cccattgcc gggttttac ctcgacccg 
      tacgcgcgc tcagacgag aaaataacc atccagcaa 
      agcaacggt tggatggtc ggcatccaa agtcaaagc 
      aaaatcacc gcgactaaa ccgttagca gccctcatc 
      ttcaacttc gagccccct tcaggcaac aaacaatct 
      cacttgacg ttcaaagaa gtcaaacac ttgttccac 
      aagtttggc ttagccctc cagcattta ctcactcga 
      accacgtac tcagaagtc tcagggtcc tcgaatatt 
      gagtgggat gcggtcgaa gtcagtggc cacgtcctg 
      gcccactgg ctgtacaat aaggagata atgcagaac 
      atttcttgc cattgtcac agtcgggag aaactcccc 
      gaaagtatg tttgattgc ttgatggag gcgcacaag 
      cacttggcc gggattgac ttatcgaga gagctgtat 
      ttggcggcg cttgattta gaactgcac cattcgacc 
      gagtattgg ctggaaata gtgaagaag ttgtggccg 
      cagttccga tgctttcct cttgataaa ctcgactcg 
      catccttgt tcctttacg cagattttt acagaggag 
      tgggctgcg gcttattac tcgcacgtc tggtcgagg 
      atgattgcc gctgatgtg tacagtgcg tttcatgaa 
      gtgcaggga gatgaccaa aaaattaga gatgtagtt 
      gccatccta gtaaagttt ttaggaagt ttagaggac 
      gggatccgt cgccgaatg cgttgctta ggtcgctag
    mRNA sequence
    • >TC010332 RA
      aggaaatat gtaaataga aaaaaagaa aaaagacgc 
      agagtagaa tctaatgag agagaattt gtttgcctt 
      tttattttt taccaagct tgtttgaaa attacaagt 
      ggtacagtt ggcgacact accaaaact tgataaatc 
      atggtctgc aacgtgaaa atcttattt agtaaaagt 
      cactaagtt gaccgccga ccccgtgcg gtaaaaatg 
      ttgaggtta tatttttaa aataatttt caataaaac 
      aactaacaa tggcattaa cactgttgg gaaagcgta 
      tcttgtcaa gacggccac aacttctca actccatta 
      gaaaaaatg gctacatcg ttttggtgc cagaaatcg 
      gtgaagact tacccgaga aaaaccccc tcctccaga 
      acgacggct tgcccgaat tcagcaaca tcaccattg 
      aaaattgca tggcagcaa tcgccaaac aaacgctcg 
      acttggagg ccggtgtga aagaaatcg aaacatcgt 
      taagcgaaa ccggaacca ctgacattt tccacgacg 
      tgtttcaag tcctggagg agatcgtgg cgcccttgg 
      acctaacct gggggctat caaaaacgc tctacttag 
      gtaacagta gtctaatgc ccaccaaaa gctacttgg 
      ccatccacg accgcgcaa aacgggccc gtgccgcga 
      aattcaaca gtccggtga tttacgagt cggttaaaa 
      gggaactgc agggcgaag ccccccaca gtgaggagg 
      aaactagga tattgaaca aattcgctt tggagggca 
      aactaaacg gtctggaac tcaccgact cgcagaaag 
      tccagctca agcaacaca ttaacaaac tcacagctg 
      agcggttgc agttcaagc aaaaagtcg acatttgta 
      ccaaacaat tttctcaca ccatccggg actcgtcga 
      ttgtgcgcg attttccgc tagatttgc taaaaacga 
      cggcttcaa atccgttaa aaccgctcg atggcccct 
      ggaaggtga ctctggacc cccatgttt acttgccga 
      ttatggaac attgccctg aacgtgaaa tacggtgga 
      atgtctggc aggcgttgg taagtcggg gctcgggtt 
      atggggatc gtgatttgt caacggggg tccatttgg 
      aggaaatta ggtttgcga gacgcgatt tagctaaat 
      tgttaggtt ttgaaacgt atgctgatc tttcaatgg 
      agacgaaaa tggcagaga atgttggta atgtgaaca 
      gtatgttgc atactctcc ttgagaagg ctaagccgg 
      cacaagaga ctgaactgg ccagtttgt acgaatttg 
      ccctcgaaa gggggttca aggggggta ggatcgaac 
      tgtgggacg ttccgtatt ggaggaaga aacagctca 
      aaacactgt tcaattaca acgaggagg agttcaaga 
      attattttc ccctaccca cggttttaa ccggtttgt 
      ttgagctgt gtgagagat tgttcaatg tggtgatca 
      aacaacgaa acgacatat ctacctggc acaaagacg 
      tcaaatttt acgatattt tcgagcccc agacaagcg 
      cccccattg ccgggtttt acctcgacc cgtacgcgc 
      gctcagacg agaaaataa ccatccagc aaagcaacg 
      gttggatgg ttggcatcc aaagtcaga gcaaaatca 
      ccgcgacta aaccattag cagccctca tcttcaact 
      ttgagcccc cttcaggca acaaacaat ctcacttga 
      cgttcaaag aagtcaaac acttgttcc acaagtttg 
      gcttagccc tccagcatt tactcactc gaaccacgt 
      actcagaag tctcagggt cctcgaata ttgagtggg 
      atgcggtcg aagtcagtg gccacgtcc tggcccact 
      ggctgtaca ataaggaga taatgcaga acatttctt 
      gccattgtc acagtcggg agaaactcc ccgaaagta 
      tgtttgatt gcttgatgg aggcgcaca agcacttgg 
      ccgggattg acttatcga gagagctgt atttggcgg 
      cgcttgatt tagaactgc accattcga ccgagtatt 
      ggctggaaa tagtgaaga agttgtggc cgcagttcc 
      ggtgcttcc ctcttgata aactcgact cgcatcctt 
      gttccttca cgcagattt ttacagagg agtgggctg 
      cggcttatt actcgcacg tctggtcga ggatgattg 
      ccgctgatg tgtacagtg cgtttcatg aagtgcagg 
      gagatgacc aaaaaatta gagatgtag ggaagagat 
      atagggaaa cgtttttgg ccttgggag gtagttgcc 
      atcctagta aagttttta ggaagttta gaggacggg 
      atccgtcgc cgaacgcgt tgcttagag ggttcaagg 
      ggggtagaa tcgaactgt gggacgtcc cgtattgga 
      ggaagaaac agctcaaaa cactgttcg attacaacg 
      aggaggagt tcaagaatt attttccct tgcccacgg 
      ttttaaccg gcttgtttg agctgtgtg agagattgt 
      tcaatgtgg tgatcaaac aacgaaacg acatatcca 
      cctggcaca aagacgtca aattctacg atattttcg 
      agccccaga caagcgccc ccattgccg ggttttacc 
      tcgacccgt acgcgcgct cagacgaga aaataacca 
      tccagcaaa gcaacggtt ggatggtcg gcatccaaa 
      gtcaaagca aaatcaccg cgactaaac cgttagcag 
      ccctcatct tcaacttcg agccccctt caggcaaca 
      aacaatctc acttgacgt tcaaagaag tcaaacact 
      tgttccaca agtttggct tagccctcc agcatttac 
      tcactcgaa ccacgtact cagaagtct cagggtcct 
      cgaatattg agtgggatg cggtcgaag tcagtggcc 
      acgtcctgg cccactggc tgtacaata aggagataa 
      tgcagaaca tttcttgcc attgtcaca gtcgggaga 
      aactccccg aaagtatgt ttgattgct tgatggagg 
      cgcacaagc acttggccg ggattgact tatcgagag 
      agctgtatt tggcggcgc ttgatttag aactgcacc 
      attcgaccg agtattggc tggaaatag tgaagaagt 
      tgtggccgc agttccgat gctttcctc ttgataaac 
      tcgactcgc atccttgtt cctttacgc agattttta 
      cagaggagt gggctgcgg cttattact cgcacgtct 
      ggtcgagga tgattgccg ctgatgtgt acagtgcgt 
      ttcatgaag tgcagggag atgaccaaa aaattagag 
      atgtagttg ccatcctag taaagtttt taggaagtt 
      tagaggacg ggatccgtc gccgaatgc gttgcttag 
      gtcgctagg tttaaagaa aagtaagat agagag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY