Status
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Gene information
General information
TC032593
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010374,TC010375
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24684209..24874460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032593
    iBeetle screen iB_09020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032593 RA
      MSFMKKMVA AESGVGTTT AEAADGGTT FGKLRQTLS 
      SSLLTAQDK VTKMSPRPS LIPDIEPSP PADEKPPVT 
      MAPPTPPVV KPEPGKPPS RAGACRVCL KAFKPDDFS 
      RTCAECTQR VCEDCASYS KLAEDEDPT NWTCSVCRR 
      KLQSRAALA QDSSESLLD VPIQELQRR HSEARLGSG 
      GGLTTGMGS GLAPPRSPE LRRHSDVSP ASLKELEKL 
      KGGGSKTPD PDWTKGRGG RSTASSRQN SPPRETAPI 
      IQAPRSRRG SRVARQLSY DDEVKNNLA PTTVGDTGL 
      GLPAPMPRR ASAYDVYAV PGLSTVTAP SVAGRRPSF 
      RVPAAETTS PPSPETAPV TLTAPEEDR RTRRRGSQL 
      PDIAGLRTM PAPRPTPAL EDLEAAPRR QASVDAEAI 
      RIVIHDVDS GSTAAAQRR VILRRDPTD KAHRTRGFG 
      MRVVGGKTA PDGRLFAYI VWTVPGGPA EKGGLQQGD 
      KVLEWGGVS LIDRSFEEV CSIMDRTGD VVELLVEHA 
      TDLRMCDLL DDPIPNMNR KPSDAAITA LHVEPETDK 
      TPSSPTRRK LPKTPEQLA RERTVSGRI QIQVWYHDE 
      RKELVVAVL AADDLAPRD ESLGFGSLP EAYARLCLM 
      PLTVFTVLD LKLHLKAFL DDRAVWCRL EDPRQLRGK 
      SPHTSPRGS IAGAGKRND FGNQRSISD DVDSIGECA 
      SLLHPDHAW GSRRGSSQS EQLEVEVYQ LGKDFSRSL 
      PGSRRSSFQ SAQEQAEQE GPAVPPPAS YSRDRRRSS 
      CTRMLRDPE EILKSLKAV KGELGRTMS LSAEKRHRR 
      STSGASNRE DRKNSVMEI TETGKESSP PSSPERTSP 
      PRLGPGQIK PRGFRLSST RPVELKLGL LMTKGQLEV 
      EVVCARHIA PRETPPDTY VKCYLRDGE RWLQKKKTR 
      VVRHSCEPQ FRQTLKYQA CDVLGRSLV VMLWERQGG 
      FEHNQGLGG AEVALDSLT LTHLTTGWY PLFPIHSLG 
      SDSNDSP
    CDS sequence
    • >TC032593 RA
      atgtccttt atgaaaaaa atggtggcg gccgagtct 
      ggagttgga acaaccacc gccgaagcg gctgatgga 
      ggaacaaca ttcggaaaa ttgagacaa acactttct 
      tcctcctta ctgacggct caggataaa gtgacgaaa 
      atgtcaccg agaccgtca ttaataccg gatatcgag 
      ccgtcaccc cctgccgat gaaaaaccg ccggtaacg 
      atggcgcct ccaactcct cccgtggtt aaaccggag 
      cccggaaaa cccccaagt cgagcggga gcgtgccgg 
      gtgtgcctc aaggctttt aaacccgac gatttcagc 
      aggacgtgt gccgaatgt acgcaacga gtctgcgag 
      gactgcgcc agttatagc aaactcgct gaagatgag 
      gatcccacc aactggaca tgcagcgtt tgtaggagg 
      aaattgcaa tcaagggcg gcattggct caagactcg 
      tcggagagt ttgttggac gttccaata caagaatta 
      cagagaagg cattccgag gcgcggctg ggatccggg 
      ggcggcttg acgacggga atggggagc ggtttagcc 
      ccacctcgt agtccggaa cttagaagg cactcggat 
      gtgtcccct gcttcgctc aaggaactg gaaaagttg 
      aaaggcggc ggctccaag acccccgac cccgactgg 
      accaaaggt cgaggaggt cgcagtacg gccagcagc 
      cgccagaac agcccccct cgcgagaca gcccccata 
      atccaagcg ccccgatca cgtcggggc tcaagagtg 
      gcaagacaa ctcagctac gacgacgaa gtgaaaaac 
      aacctcgca cctacgacg gtgggggac accggtctg 
      ggcttgcca gcccccatg cccaggagg gcttccgct 
      tacgacgtc tacgcagtg cccgggctg agcaccgtg 
      acggcgccg tcggtcgcc ggaagacgg ccgtcgttt 
      agagtaccc gcagctgaa accaccagt cccccaagt 
      ccggaaaca gcccccgtc acgttaaca gccccagag 
      gaagacagg cggaccagg agaagggga tcgcaactg 
      ccggacata gccggattg cgcacaatg ccagcccct 
      cgaccgact ccagcttta gaggattta gaagcggcc 
      ccgaggcgg caagcttcg gtggacgcg gaggccatt 
      cgaatcgtc atccacgac gtggattca ggctcgacg 
      gccgccgcc cagagacga gttatcctc aggagagac 
      cccaccgac aaggcacac agaacccgc ggcttcgga 
      atgcgcgtc gtaggcgga aagacagca ccggatggc 
      cgccttttt gcgtacatc gtctggact gtccctggc 
      ggaccagct gaaaaagga ggtctgcag cagggcgat 
      aaagtgctt gaatgggga ggcgtctct ctcatcgac 
      cgctcgttt gaggaagtg tgttctata atggaccgc 
      acaggtgac gtggttgag cttttggtc gaacatgcg 
      accgacttg agaatgtgc gaccttttg gatgacccc 
      attcccaac atgaaccgg aagccgtcg gatgccgcg 
      atcacggcg ctgcacgtc gaaccggaa actgacaag 
      acgccttcg tcgccgaca agacggaaa cttcctaaa 
      acgccggag caacttgcg agggagcgc accgtctca 
      ggaagaata caaatccaa gtttggtat cacgatgaa 
      cggaaggag ttggtagtg gcagtcctg gctgcagat 
      gatttggcc ccgcgagac gaaagtctc ggttttgga 
      tcacttccc gaagcttac gcccgctta tgtttaatg 
      cctttgacg gtgtttacg gttttggac ttgaaactt 
      cacctgaaa gccttcctg gacgaccgg gccgtctgg 
      tgccgcctg gaggacccc cgacagctg cgcggcaaa 
      tccccgcac acgtcccca cgagggtcg atagctggt 
      gctggcaaa cgcaacgac ttcggcaac cagaggagc 
      atctccgac gacgtcgac tccatcgga gagtgcgcc 
      agcctcctg cacccggac cacgcgtgg ggctcccgg 
      cgcggctcc agccagtcg gagcagctg gaggtggag 
      gtgtaccag ctggggaag gacttctcc aggtcgctg 
      ccgggctcc aggaggtcg tccttccag tcggcgcag 
      gagcaagcg gagcaggag gggccggcg gtgccgccc 
      ccagcgtcc tacagcagg gaccggagg cggagcagc 
      tgcaccagg atgctgagg gacccggag gagatactc 
      aagtcgctg aaggcggtc aagggggag cttggacgg 
      actatgagc ttatcggcg gaaaagaga cacagaaga 
      tcgaccagc ggcgcttcg aaccgcgag gatcgcaag 
      aacagcgtg atggagatc accgagacc ggcaaggag 
      agcagcccc ccttcgagc ccggagcgc acgtcgccc 
      cctcgcctg ggccccggc cagatcaag ccgcggggc 
      ttccgcctg tccagcacc cgcccggtg gagctgaag 
      ctggggctg ctgatgacc aagggccag ctggaggtg 
      gaggtggtg tgcgcgcgc cacatcgcc ccccgggag 
      acgcccccc gacacctac gtcaagtgc tacctgcgc 
      gacggcgag cgctggctg cagaagaag aagacgcgg 
      gtggtgcgc cactcctgc gagccccag ttccggcag 
      acgctcaag taccaggcg tgcgacgtg ctggggcgc 
      agcctcgtc gtcatgctg tgggagcgc caggggggc 
      ttcgagcac aaccagggc ttggggggc gccgaggtg 
      gcgctggac tcgctcacg ctcacgcat ctcactacc 
      ggctggtac ccgctcttt cccatacat tcgctcgga 
      tccgattcg aacgattcg ccttga
    mRNA sequence
    • >TC032593 RA
      ttccgggaa ctaacaaag ctcgtttcc tcggctctg 
      gttgtgata tgtgcgtga ttgcgtcgt gcagtggct 
      ttcttcacc cttgcagct cctgccaca ggttgctgt 
      taacacttg ctggtgtct tctcagatg aaatatgaa 
      gatgcagcg gacaaagaa aaaaaccct aaaatgtga 
      aaagttcat acttcgcta aaattcagc gcttagttt 
      actgtaaga aagctttgg acaatacgc cgccatgtt 
      gcctacaaa tgttatgtc ctttatgaa aaaaatggt 
      ggcggccga gtctggagt tggaacaac caccgccga 
      agcggctga tggaggaac aacattcgg aaaattgag 
      acaaacact ttcttcctc cttactgac ggctcagga 
      taaagtgac gaaaatgtc accgagacc gtcattaat 
      accggatat cgagccgtc accccctgc cgatgaaaa 
      accgccggt aacgatggc gcctccaac tcctcccgt 
      ggttaaacc ggagcccgg aaaaccccc aagtcgagc 
      gggagcgtg ccgggtgtg cctcaaggc ttttaaacc 
      cgacgattt cagcaggac gtgtgccga atgtacgca 
      acgagtctg cgaggactg cgccagtta tagcaaact 
      cgctgaaga tgaggatcc caccaactg gacatgcag 
      cgtttgtag gaggaaatt gcaatcaag ggcggcatt 
      ggctcaaga ctcgtcgga gagtttgtt ggacgttcc 
      aatacaaga attacagag aaggcattc cgaggcgcg 
      gctgggatc cgggggcgg cttgacgac gggaatggg 
      gagcggttt agccccacc tcgtagtcc ggaacttag 
      aaggcactc ggatgtgtc ccctgcttc gctcaagga 
      actggaaaa gttgaaagg cggcggctc caagacccc 
      cgaccccga ctggaccaa aggtcgagg aggtcgcag 
      tacggccag cagccgcca gaacagccc ccctcgcga 
      gacagcccc cataatcca agcgccccg atcacgtcg 
      gggctcaag agtggcaag acaactcag ctacgacga 
      cgaagtgaa aaacaacct cgcacctac gacggtggg 
      ggacaccgg tctgggctt gccagcccc catgcccag 
      gagggcttc cgcttacga cgtctacgc agtgcccgg 
      gctgagcac cgtgacggc gccgtcggt cgccggaag 
      acggccgtc gtttagagt acccgcagc tgaaaccac 
      cagtccccc aagtccgga aacagcccc cgtcacgtt 
      aacagcccc agaggaaga caggcggac caggagaag 
      gggatcgca actgccgga catagccgg attgcgcac 
      aatgccagc ccctcgacc gactccagc tttagagga 
      tttagaagc ggccccgag gcggcaagc ttcggtgga 
      cgcggaggc cattcgaat cgtcatcca cgacgtgga 
      ttcaggctc gacggccgc cgcccagag acgagttat 
      cctcaggag agaccccac cgacaaggc acacagaac 
      ccgcggctt cggaatgcg cgtcgtagg cggaaagac 
      agcaccgga tggccgcct ttttgcgta catcgtctg 
      gactgtccc tggcggacc agctgaaaa aggaggtct 
      gcagcaggg cgataaagt gcttgaatg gggaggcgt 
      ctctctcat cgaccgctc gtttgagga agtgtgttc 
      tataatgga ccgcacagg tgacgtggt tgagctttt 
      ggtcgaaca tgcgaccga cttgagaat gtgcgacct 
      tttggatga ccccattcc caacatgaa ccggaagcc 
      gtcggatgc cgcgatcac ggcgctgca cgtcgaacc 
      ggaaactga caagacgcc ttcgtcgcc gacaagacg 
      gaaacttcc taaaacgcc ggagcaact tgcgaggga 
      gcgcaccgt ctcaggaag aatacaaat ccaagtttg 
      gtatcacga tgaacggaa ggagttggt agtggcagt 
      cctggctgc agatgattt ggccccgcg agacgaaag 
      tctcggttt tggatcact tcccgaagc ttacgcccg 
      cttatgttt aatgccttt gacggtgtt tacggtttt 
      ggacttgaa acttcacct gaaagcctt cctggacga 
      ccgggccgt ctggtgccg cctggagga cccccgaca 
      gctgcgcgg caaatcccc gcacacgtc cccacgagg 
      gtcgatagc tggtgctgg caaacgcaa cgacttcgg 
      caaccagag gagcatctc cgacgacgt cgactccat 
      cggagagtg cgccagcct cctgcaccc ggaccacgc 
      gtggggctc ccggcgcgg ctccagcca gtcggagca 
      gctggaggt ggaggtgta ccagctggg gaaggactt 
      ctccaggtc gctgccggg ctccaggag gtcgtcctt 
      ccagtcggc gcaggagca agcggagca ggaggggcc 
      ggcggtgcc gcccccagc gtcctacag cagggaccg 
      gaggcggag cagctgcac caggatgct gagggaccc 
      ggaggagat actcaagtc gctgaaggc ggtcaaggg 
      ggagcttgg acggactat gagcttatc ggcggaaaa 
      gagacacag aagatcgac cagcggcgc ttcgaaccg 
      cgaggatcg caagaacag cgtgatgga gatcaccga 
      gaccggcaa ggagagcag ccccccttc gagcccgga 
      gcgcacgtc gccccctcg cctgggccc cggccagat 
      caagccgcg gggcttccg cctgtccag cacccgccc 
      ggtggagct gaagctggg gctgctgat gaccaaggg 
      ccagctgga ggtggaggt ggtgtgcgc gcgccacat 
      cgccccccg ggagacgcc ccccgacac ctacgtcaa 
      gtgctacct gcgcgacgg cgagcgctg gctgcagaa 
      gaagaagac gcgggtggt gcgccactc ctgcgagcc 
      ccagttccg gcagacgct caagtacca ggcgtgcga 
      cgtgctggg gcgcagcct cgtcgtcat gctgtggga 
      gcgccaggg gggcttcga gcacaacca gggcttggg 
      gggcgccga ggtggcgct ggactcgct cacgctcac 
      gcatctcac taccggctg gtacccgct ctttcccat 
      acattcgct cggatccga ttcgaacga ttcgccttg 
      acctggtgc ggactcgcc tcctgaaga tccctccga 
      ttgaggcgc gaatctgaa tccagattc caaattggt 
      gcgaaattc ggatcaggt tggacggtc gttgcgggc 
      gagttacgg aggactata gattaggaa acgaaacga 
      ggtacaaaa acgcaccca aaaaatata gtacggtga 
      agtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY