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Gene information
General information
TC000080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11362678..11363534
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000080
    iBeetle screen iB_09011

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer sometimes fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000080 RA
      MQQQHHHHN DILQRYPEV LVSLRRARC 
      CRRLLRSAK GRLKKEKLC VRRLQSALF 
      DAMDASRNV ELFTCPLCR SVLKGPVTV 
      ECGHTFCYG CLQENCDVC GICDTEITA 
      NRCVNVLVQ DLVDKWRDT NKIAGRYYY 
      VIVRKNNDV TFTYRGQFN RAGRAVAAR 
      CAANTEKKL EILN
    CDS sequence
    • >TC000080 RA
      atgcagcag cagcaccac caccacaac gacatcctg 
      cagaggtac cccgaggtg ctggtgtcg ctgaggagg 
      gcccgctgc tgcaggagg ctgctgcgg agcgccaag 
      ggccgcctc aagaaggag aagctgtgc gtgaggcgg 
      cttcagagc gcgcttttc gacgcgatg gacgccagc 
      aggaacgtg gagcttttt acctgcccg ctctgcagg 
      agtgtgctc aaaggaccc gtcaccgtg gagtgtgga 
      cacacgttc tgttacggg tgtttgcag gagaattgt 
      gacgtttgt gggatttgt gacacggaa atcacagcc 
      aacaggtgt gtcaatgtc ctggtgcag gaccttgtc 
      gacaagtgg agagatacg aataaaatt gcaggtaga 
      tattattac gtaattgtt aggaagaac aatgatgta 
      acatttaca tatcgcggc caatttaat agagcaggg 
      agggcagtg gcggctcgt tgcgctgcc aacactgag 
      aaaaaactt gaaatatta aattaa
    mRNA sequence
    • >TC000080 RA
      cgcgtcagt tcagttttg gcctcgaac gcgagaagg 
      cacgctggt tgcctccgc cgtcgactc catgtgctc 
      aacgtatga cgccgagct tttacataa cacgaaatt 
      tttcgcgtc tgtttattg ttgaccgtg tgttacgag 
      ttacacaac gttgccact tacacaagc ggccttttc 
      gtaaattac gaataatcg gagtgattt cccggaaaa 
      atccagtgc gcaagtggc cggaaaatc gatcgatac 
      gtcttcacg tgctacatg ccacgatgc agcagcagc 
      accaccacc acaacgaca tcctgcaga ggtaccccg 
      aggtgctgg tgtcgctga ggagggccc gctgctgca 
      ggaggctgc tgcggagcg ccaagggcc gcctcaaga 
      aggagaagc tgtgcgtga ggcggcttc agagcgcgc 
      ttttcgacg cgatggacg ccagcagga acgtggagc 
      tttttacct gcccgctct gcaggagtg tgctcaaag 
      gacccgtca ccgtggagt gtggacaca cgttctgtt 
      acgggtgtt tgcaggaga attgtgacg tttgtggga 
      tttgtgaca cggaaatca cagccaaca ggtgtgtca 
      atgtcctgg tgcaggacc ttgtcgaca agtggagag 
      atacgaata aaattgcag gtagatatt attacgtaa 
      ttgttagga agaacaatg atgtaacat ttacatatc 
      gcggccaat ttaatagag cagggaggg cagtggcgg 
      ctcgttgcg ctgccaaca ctgagaaaa aacttgaaa 
      tattaaatt aaagtaaaa atataaatg gttagaaga 
      aaaaaatga aataaaaat tgctttaaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY