Status
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Gene information
General information
TC011563 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450689.1: 93245..94943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011563
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09010

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands content opaque - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    vitellogenic egg chamber early without detectable defects - Number of animals showing the phenotype: 3

    tropharium anterior potentiallystructure irregular - Number of animals showing the phenotype: 2

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011563 RA
      MTTVPNLKP PAQVAGLTT LDKNLFKKN ITVPCLILK 
      NVKISSVLP FVKNYLLKI ENFKPVCNV TTGESHVYF 
      NPEIIGAFS DLPPETQKL LNDLCVQLE LKPLTLNYE 
      NFSIEGVFR AVLPPNVEG MSSFTKVGH IVHVNLREH 
      LVPFKDIIG QVLFDKVPN CRTVVNKVG SIDNTYRNF 
      QMEVLRGEN DTQTHVREN KCVFEFDFA KVYWNSRLC 
      TEHERIVNM IESGDVVFD VFAGVGPFS VPLARKKCQ 
      VFANDLNPE SFKWLNHNF KINKVGENY FKSYNKDGR 
      EFILGEVKE LLPKFSAKN VFILMNLPA LAVDFLTTF 
      VDLFSNDEL PEFGKHPVV VVYCFAKGE DFINIAKKS 
      LCEKIGRNV EEKITDVFR VRTTAKFWS KITYGNEKI 
      PALALNPKN SVMGKNKKK LNSVFKVAG AKSLKLKAK 
      AKAVKSELK QINNKNKAK ITEIDKELV NLQEKVRNS 
      TGDQKKETK NKTKLPSRQ NVTKEVGEK SQDTLKQLN 
      AMQL
    CDS sequence
    • >TC011563 RA
      atgacaact gtccctaac ctcaaacca ccagctcaa 
      gtagctggg ttaacaact ttggacaag aatttgttc 
      aagaaaaac ataaccgtc ccgtgtctc atccttaag 
      aacgtgaaa atctcatca gtcttgcca ttcgtgaaa 
      aattacttg ttaaaaatt gagaatttt aagccggtt 
      tgtaacgta accacgggg gaatcccac gtttacttc 
      aacccggaa ataataggg gcttttagt gacttgccg 
      ccagaaacg caaaaattg ctaaacgac ctgtgcgtg 
      caactcgaa ttaaagccg ttgacttta aactacgag 
      aatttttcg attgagggc gtttttcgc gccgtgctt 
      cccccgaat gtcgagggg atgtcgagt tttactaaa 
      gtggggcac attgtgcac gttaacctg cgcgaacac 
      ctcgttccg tttaaagac ataattggg caagtgttg 
      tttgataaa gtgcccaat tgtcggact gtggtcaat 
      aaagtgggg agtattgat aacacgtat cggaatttc 
      cagatggag gttttaagg ggcgaaaac gacacgcaa 
      acgcacgtt agggaaaat aaatgtgtg tttgagttt 
      gattttgcg aaagtgtac tggaattct agactctgc 
      actgaacac gaaagaatt gtcaatatg atagagagt 
      ggtgatgtt gtgtttgat gttttcgcg ggggttggg 
      ccgttttcc gtgcctttg gctaggaaa aagtgccag 
      gtttttgcc aatgatctc aatcccgag tcctttaaa 
      tggctaaat cacaacttt aaaataaac aaagttggg 
      gaaaattac tttaagagt tataataag gacgggcgg 
      gagtttatc ttgggcgag gttaaggag ttgttgccg 
      aaattttcg gccaaaaat gtttttatt ctgatgaat 
      cttccggca ctggccgtt gatttttta acaactttt 
      gttgattta ttctcaaat gatgagtta ccagaattt 
      gggaaacac ccagttgtc gtagtttat tgctttgcc 
      aaaggcgag gatttcata aacattgcc aaaaaatca 
      ctttgtgaa aaaatcggt cggaatgtt gaagaaaaa 
      attaccgat gtgtttcgt gtgcgcacc accgcgaaa 
      ttttggtca aaaattacg tacggaaac gaaaaaatt 
      cctgcgttg gcgttgaat ccaaaaaac tctgtgatg 
      gggaaaaac aagaagaag ctcaatagt gtgttcaag 
      gtcgccggg gccaaaagc ttgaagctc aaggccaag 
      gcgaaagct gtcaaaagt gagctcaaa cagattaat 
      aataaaaat aaggccaag ataacggaa attgataag 
      gaactcgtc aacttgcag gaaaaagtc cgaaattcg 
      accggtgac cagaagaaa gaaactaaa aataaaacc 
      aaattaccg tcacgtcag aatgtgacg aaggaggtt 
      ggtgaaaag tcgcaagac actttgaag caattgaat 
      gccatgcag ttgtag
    mRNA sequence
    • >TC011563 RA
      caaaagcat gacaactgt ccctaacct caaaccacc 
      agctcaagt agctgggtt aacaacttt ggacaagaa 
      tttgttcaa gaaaaacat aaccgtccc gtgtctcat 
      ccttaagaa cgtgaaaat ctcatcagt cttgccatt 
      cgtgaaaaa ttacttgtt aaaaattga gaattttaa 
      gccggtttg taacgtaac cacggggga atcccacgt 
      ttacttcaa cccggaaat aataggggc ttttagtga 
      cttgccgcc agaaacgca aaaattgct aaacgacct 
      gtgcgtgca actcgaatt aaagccgtt gactttaaa 
      ctacgagaa tttttcgat tgagggcgt ttttcgcgc 
      cgtgcttcc cccgaatgt cgaggggat gtcgagttt 
      tactaaagt ggggcacat tgtgcacgt taacctgcg 
      cgaacacct cgttccgtt taaagacat aattgggca 
      agtgttgtt tgataaagt gcccaattg tcggactgt 
      ggtcaataa agtggggag tattgataa cacgtatcg 
      gaatttcca gatggaggt tttaagggg cgaaaacga 
      cacgcaaac gcacgttag ggaaaataa atgtgtgtt 
      tgagtttga ttttgcgaa agtgtactg gaattctag 
      actctgcac tgaacacga aagaattgt caatatgat 
      agagagtgg tgatgttgt gtttgatgt tttcgcggg 
      ggttgggcc gttttccgt gcctttggc taggaaaaa 
      gtgccaggt ttttgccaa tgatctcaa tcccgagtc 
      ctttaaatg gctaaatca caactttaa aataaacaa 
      agttgggga aaattactt taagagtta taataagga 
      cgggcggga gtttatctt gggcgaggt taaggagtt 
      gttgccgaa attttcggc caaaaatgt ttttattct 
      gatgaatct tccggcact ggccgttga ttttttaac 
      aacttttgt tgatttatt ctcaaatga tgagttacc 
      agaatttgg gaaacaccc agttgtcgt agtttattg 
      ctttgccaa aggcgagga tttcataaa cattgccaa 
      aaaatcact ttgtgaaaa aatcggtcg gaatgttga 
      agaaaaaat taccgatgt gtttcgtgt gcgcaccac 
      cgcgaaatt ttggtcaaa aattacgta cggaaacga 
      aaaaattcc tgcgttggc gttgaatcc aaaaaactc 
      tgtgatggg gaaaaacaa gaagaagct caatagtgt 
      gttcaaggt cgccggggc caaaagctt gaagctcaa 
      ggccaaggc gaaagctgt caaaagtga gctcaaaca 
      gattaataa taaaaataa ggccaagat aacggaaat 
      tgataagga actcgtcaa cttgcagga aaaagtccg 
      aaattcgac cggtgacca gaagaaaga aactaaaaa 
      taaaaccaa attaccgtc acgtcagaa tgtgacgaa 
      ggaggttgg tgaaaagtc gcaagacac tttgaagca 
      attgaatgc catgcagtt gtagatcat aaaataaat 
      cgtttatta cgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY