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Gene information
General information
TC001368 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15105641..15107621
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001368
    iBeetle screen iB_09004

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001368 RA
      MAVSLYEHY TSGGVTSGL STNQSYFTL NSKNITLYS 
      GALHYFRVP QQYWRDRLR KLRAAGLNT VETYVPWNL 
      HEPQIGNYD FGDGGSDFS NFLHLEKFL KLAQEEDLL 
      AIVRPGPYI CAEWDFGGL PSWLLRDNV KVRTSEPKF 
      MSHVTRFFT RLLPILAAL QFTKGGPIV AFQVENEYG 
      STEELGKFA PDKLYIKQL SDLMRKFGL VELLFTSDS 
      PSQHGDRGT LPELFQTAN FARDPGKEF QALGEYQKS 
      RPTMAMEFW TGWFDHWGE GHNRRNNTE FSLVLNEIL 
      KYPASVNMY MFHGGTSFG FLNGANVPY QPDTTSYDY 
      DAPLTENGN YTEKYHIVK NLITRQDGI KTKVPPLPD 
      VIPTHWYPK LRIGQQLKY HDIIKQLEI ALVSEKVVP 
      MELLPINNN SGQSYGYTI YRTTSDIPP NSTLKIEGY 
      IHDSCLILV DDELISPQL NNSSDLDKF GYWRLKDGT 
      IQLPQKPNA TIDIIVENW GRINYGKLH QFKQFKGLN 
      QNIFLNDQE LKNWTIIPL EFKSNWTNS LTGWGQHAR 
      VNLTLNKAI FPVSDPEDT YLDMSDWNT GIVIVNGFV 
      LGRFMHLGP QKTLYLPAP LLRKGNNEI VIFEHFIAN 
      TFVTFAKDP IYV
    CDS sequence
    • >TC001368 RA
      atggcagtc tcactttac gaacactac acttccgga 
      ggtgtaaca tctggttta tccaccaac cagtcgtac 
      ttcaccctt aattcgaaa aatatcact ctgtatagt 
      ggcgcttta cattatttc cgggtacca cagcagtac 
      tggagggac cgtctccga aaattacga gccgctggc 
      ttgaacacc gtcgaaact tacgttccg tggaattta 
      cacgaaccc caaattggt aattatgat tttggagac 
      ggtggcagc gactttagt aattttctc cacctcgaa 
      aagttccta aagttggca caagaggag gatttgttg 
      gcaatcgtg cgtccagga ccgtacatt tgtgccgag 
      tgggatttc gggggttta cccagttgg ttacttcgc 
      gacaatgtc aaagttagg acttcagag ccaaaattt 
      atgtcacac gtgactcgt ttttttacc cgtttattg 
      cccattttg gccgcgctt cagtttact aaaggtggt 
      ccaattgtt gcgtttcaa gtcgaaaat gaatatggt 
      agtaccgaa gaactgggc aagttcgcc cctgataag 
      ctttacatc aaacaattg agtgattta atgcgaaaa 
      ttcggtctt gttgaacta cttttcaca tcagattcg 
      ccaagccaa catggcgac cgtggtact ttacctgag 
      ctttttcaa acggcgaat ttcgcgcgt gatccaggc 
      aaagaattc caagcttta ggggagtac caaaagtca 
      cggcccact atggcaatg gaattttgg actgggtgg 
      tttgaccat tggggtgaa ggtcacaat aggcgtaat 
      aacaccgaa ttttcgttg gttctgaac gaaattctc 
      aaatatcca gcttcggtt aacatgtac atgtttcat 
      ggtggtacc agttttggg tttttgaat ggtgccaat 
      gtcccctac caacctgat accacaagt tatgactat 
      gacgcacct ttgaccgaa aatggcaac tacactgag 
      aagtaccat attgttaaa aatctaatc acgcgacaa 
      gatggcatc aagacaaaa gtaccgcca ttgcctgac 
      gtaatacca actcattgg tacccaaaa ttaagaatt 
      gggcaacaa ttaaagtac catgacata atcaaacaa 
      cttgaaatt gccttagtg agtgaaaaa gtggtacca 
      atggaactc ctcccgatt aacaataat tcgggtcaa 
      agttacggc tacacaatt tacagaacc acaagtgat 
      attccccca aatagtacc ctcaaaatc gaaggctat 
      attcacgac tcttgttta attttggtt gatgatgaa 
      ttaatttcg ccacaactt aacaatagt tcagacctt 
      gataaattc gggtattgg cgattgaaa gacggtact 
      atccaacta ccacaaaaa ccaaatgct accattgac 
      ataatcgtg gaaaattgg ggccgaatc aattacgga 
      aaattgcac caatttaaa cagttcaaa ggtttgaac 
      caaaatatt tttttgaat gatcaggag ttaaaaaac 
      tggaccata ataccactg gaattcaag tcaaattgg 
      accaattct ttgacgggt tggggccaa catgctcgc 
      gttaacctg actttgaac aaggctatt ttcccggtc 
      agtgacccc gaagacact tatctcgat atgtctgat 
      tggaacacc ggaattgtg attgtaaac ggtttcgtt 
      ttgggccgt tttatgcac ttagggcca caaaaaact 
      ctatacctt ccagccccc ttattacgt aaaggaaac 
      aacgaaatt gtgattttt gaacacttt atagcaaac 
      acttttgta acgttcgca aaagatcca atctatgta 
      taa
    mRNA sequence
    • >TC001368 RA
      ccgccactg ttaccaata aaaattccg gttccacct 
      caaacctgt ttattgtca tttattgtg ttgtgtgtt 
      tacgtgtga tggcagtct cactttacg aacactaca 
      cttccggag gtgtaacat ctggtttat ccaccaacc 
      agtcgtact tcaccctta attcgaaaa atatcactc 
      tgtatagtg gcgctttac attatttcc gggtaccac 
      agcagtact ggagggacc gtctccgaa aattacgag 
      ccgctggct tgaacaccg tcgaaactt acgttccgt 
      ggaatttac acgaacccc aaattggta attatgatt 
      ttggagacg gtggcagcg actttagta attttctcc 
      acctcgaaa agttcctaa agttggcac aagaggagg 
      atttgttgg caatcgtgc gtccaggac cgtacattt 
      gtgccgagt gggatttcg ggggtttac ccagttggt 
      tacttcgcg acaatgtca aagttagga cttcagagc 
      caaaattta tgtcacacg tgactcgtt tttttaccc 
      gtttattgc ccattttgg ccgcgcttc agtttacta 
      aaggtggtc caattgttg cgtttcaag tcgaaaatg 
      aatatggta gtaccgaag aactgggca agttcgccc 
      ctgataagc tttacatca aacaattga gtgatttaa 
      tgcgaaaat tcggtcttg ttgaactac ttttcacat 
      cagattcgc caagccaac atggcgacc gtggtactt 
      tacctgagc tttttcaaa cggcgaatt tcgcgcgtg 
      atccaggca aagaattcc aagctttag gggagtacc 
      aaaagtcac ggcccacta tggcaatgg aattttgga 
      ctgggtggt ttgaccatt ggggtgaag gtcacaata 
      ggcgtaata acaccgaat tttcgttgg ttctgaacg 
      aaattctca aatatccag cttcggtta acatgtaca 
      tgtttcatg gtggtacca gttttgggt ttttgaatg 
      gtgccaatg tcccctacc aacctgata ccacaagtt 
      atgactatg acgcacctt tgaccgaaa atggcaact 
      acactgaga agtaccata ttgttaaaa atctaatca 
      cgcgacaag atggcatca agacaaaag taccgccat 
      tgcctgacg taataccaa ctcattggt acccaaaat 
      taagaattg ggcaacaat taaagtacc atgacataa 
      tcaaacaac ttgaaattg ccttagtga gtgaaaaag 
      tggtaccaa tggaactcc tcccgatta acaataatt 
      cgggtcaaa gttacggct acacaattt acagaacca 
      caagtgata ttcccccaa atagtaccc tcaaaatcg 
      aaggctata ttcacgact cttgtttaa ttttggttg 
      atgatgaat taatttcgc cacaactta acaatagtt 
      cagaccttg ataaattcg ggtattggc gattgaaag 
      acggtacta tccaactac cacaaaaac caaatgcta 
      ccattgaca taatcgtgg aaaattggg gccgaatca 
      attacggaa aattgcacc aatttaaac agttcaaag 
      gtttgaacc aaaatattt ttttgaatg atcaggagt 
      taaaaaact ggaccataa taccactgg aattcaagt 
      caaattgga ccaattctt tgacgggtt ggggccaac 
      atgctcgcg ttaacctga ctttgaaca aggctattt 
      tcccggtca gtgaccccg aagacactt atctcgata 
      tgtctgatt ggaacaccg gaattgtga ttgtaaacg 
      gtttcgttt tgggccgtt ttatgcact tagggccac 
      aaaaaactc tataccttc cagccccct tattacgta 
      aaggaaaca acgaaattg tgatttttg aacacttta 
      tagcaaaca cttttgtaa cgttcgcaa aagatccaa 
      tctatgtat aaaaaatta ataaaaatt gtattacta 
      c
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