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Gene information
General information
TC001296 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14104455..14105852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001296
    iBeetle screen iB_09003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001296 RA
      MFPKSPENY IIREKYQKI YLLSPGSLT PPPGSEVFV 
      GNIPSVISD DELIFIFSE AGRVYRFQR MIDDNGNNK 
      PFAFISYHN DEDANKAVL FFHKFSIRK HRKLNVHLS 
      VNNCRLYFG NIPREMTKE DVMLNLLNF IEKIVKVLV 
      LPSRCNTAL NRGYAFVDF VSHTLAAFA KRQLELGLF 
      WGGRHITVE WAYREPLVP PFIFLQIKC LIFKNIPID 
      CSRCVFRKF LSLFVHITT VRRIYKKDS HAFVHFQSW 
      EAAEIAIKT LRSHILRIY DREIRVEWA RPPNYRRKK 
      TIPDHFSKF VCPQVQNCQ EEQKKERKG EAVRFKCRK 
      RGDLRPIS
    CDS sequence
    • >TC001296 RA
      atgttccca aaaagcccc gaaaactat ataattcgt 
      gaaaaatac caaaaaatc tacttatta agccccggg 
      tcgttaaca cctcctcct ggaagtgaa gtatttgtc 
      ggcaatata ccaagtgtg atatcagac gatgagctc 
      atcttcatc ttctccgaa gcgggtcgc gtttacaga 
      ttccagcga atgatagac gacaatggc aataacaaa 
      ccatttgca tttatcagt tatcacaac gatgaagac 
      gcgaacaaa gctgttttg ttcttccac aaattttca 
      atacgaaag cacaggaaa ttaaatgtt cacctgtcg 
      gtgaataac tgccgactg tactttgga aacattccg 
      agagaaatg accaaggaa gacgtcatg cttaacctg 
      ttaaatttt atcgaaaaa attgttaaa gtcctagtc 
      ctaccatca agatgtaat acggcattg aatcgtggt 
      tatgctttt gtggacttt gtatcgcat acattggct 
      gccttcgcc aaaaggcaa ctagaattg gggcttttc 
      tggggtggg aggcacatt acagtggag tgggcttac 
      cgtgagcct ttggtgccc cctttcatt tttctacaa 
      ataaaatgc ctcattttc aagaatatc cccatcgat 
      tgttcgcgg tgcgttttc aggaaattt ctcagcctg 
      tttgtccac atcaccacc gtccgaagg atttacaaa 
      aaagatagt catgctttc gtccacttc caatcatgg 
      gaagcggcc gaaatagct atcaaaaca ttacgctca 
      catattttg aggatttac gatagggaa attagagtg 
      gaatgggcc agaccgcca aactaccgt cgcaaaaaa 
      acaattccg gatcatttt tccaagttt gtatgtccg 
      caagttcaa aattgccag gaggagcaa aagaaagaa 
      cggaaagga gaagccgtt cgtttcaag tgccgcaaa 
      cgaggcgat ttgagacca atcagttaa
    mRNA sequence
    • >TC001296 RA
      gtgataacg agtgcatcg tgcagttaa aaatgttcc 
      caaaaagcc ccgaaaact atataattc gtgaaaaat 
      accaaaaaa tctacttat taagccccg ggtcgttaa 
      cacctcctc ctggaagtg aagtatttg tcggcaata 
      taccaagtg tgatatcag acgatgagc tcatcttca 
      tcttctccg aagcgggtc gcgtttaca gattccagc 
      gaatgatag acgacaatg gcaataaca aaccatttg 
      catttatca gttatcaca acgatgaag acgcgaaca 
      aagctgttt tgttcttcc acaaatttt caatacgaa 
      agcacagga aattaaatg ttcacctgt cggtgaata 
      actgccgac tgtactttg gaaacattc cgagagaaa 
      tgaccaagg aagacgtca tgcttaacc tgttaaatt 
      ttatcgaaa aaattgtta aagtcctag tcctaccat 
      caagatgta atacggcat tgaatcgtg gttatgctt 
      ttgtggact ttgtatcgc atacattgg ctgccttcg 
      ccaaaaggc aactagaat tggggcttt tctggggtg 
      ggaggcaca ttacagtgg agtgggctt accgtgagc 
      ctttggtgc cccctttca tttttctac aaataaaat 
      gcctcattt tcaagaata tccccatcg attgttcgc 
      ggtgcgttt tcaggaaat ttctcagcc tgtttgtcc 
      acatcacca ccgtccgaa ggatttaca aaaaagata 
      gtcatgctt tcgtccact tccaatcat gggaagcgg 
      ccgaaatag ctatcaaaa cattacgct cacatattt 
      tgaggattt acgataggg aaattagag tggaatggg 
      ccagaccgc caaactacc gtcgcaaaa aaacaattc 
      cggatcatt tttccaagt ttgtatgtc cgcaagttc 
      aaaattgcc aggaggagc aaaagaaag aacggaaag 
      gagaagccg ttcgtttca agtgccgca aacgaggcg 
      atttgagac caatcagtt aaaagtgcc aattttctc 
      tgaaataat tggctgcac cttcaccca gttttaaga 
      gattcgaat cattttgtt catttatcc ataattttt 
      aagttttgc atgtttttt cgatttttt aattaaaat 
      cacttatgt ttgttttac ttttcctac gaataaaat 
      cagttatat ggg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY