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Gene information
General information
TC000838 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6593927..6595971
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000838
    iBeetle screen iB_08994

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000838 RA
      MNKPWKKYL LWFAQEHIE FRFEEIDSL LSMFQIKMR 
      HFKEPDNTA PYWIVEFES ESDLKRLVS RSVSIRSCM 
      ELWAHAKDI QSLHCQLQN FPKQLMEPY FQPHLSFKI 
      EVETFCRHF SQKEKINKL ESFSYLPVQ GPVKLKDPD 
      VCLHYIEYY GTRANDPPE NPYDVFFGR WIANGLRQL 
      IRKLSLKTR KFIGNTSMD PQLSLLMAN QAQVKDGDI 
      VLDPFVGSG SLLVAAAEF GGFVLGGDI DYLMLHART 
      RPSRIKQKE RAADESIKA NMEQYNLQH KYLDVLIND 
      FASSFWRDD VEFDSIITD PPYGIREAT ERVGTSKEN 
      FTVSEKHLP THIPAKIEY GISSIYNDL LKFAAKHLR 
      LGGRLVCWF PVFREDYSE AVNKS
    CDS sequence
    • >TC000838 RA
      atgaataaa ccgtggaaa aaatacctc ctgtggttt 
      gcacaggaa catatcgaa tttcgcttc gaagagatt 
      gattcgtta ctttccatg tttcaaatt aaaatgaga 
      cacttcaaa gaaccagat aataccgcg ccttattgg 
      atcgttgaa tttgagtca gaaagtgat ttgaaacgt 
      ttagttagc agatctgtc tcgattaga tcatgtatg 
      gaattgtgg gcacatgcg aaagatatc cagagttta 
      cactgtcag ttacagaat ttcccaaaa cagttaatg 
      gagccttac tttcaaccc catttgtct ttcaaaatt 
      gaagtggaa actttttgt cgccacttc agccaaaag 
      gaaaaaatt aataagctt gaaagtttt agttatctg 
      cctgtccaa ggccctgtt aaattaaaa gatccagat 
      gtttgttta cattacatt gaatattac ggaacaagg 
      gctaatgac ccacctgaa aacccttac gatgtgttt 
      tttggcaga tggatagct aatggtttg agacaatta 
      atcaggaaa ttgtcgtta aaaactcgg aaatttatt 
      ggaaacact agtatggat ccacagttg tctttattg 
      atggctaat caagcccaa gtcaaggat ggggacatt 
      gtcttagac ccctttgta ggctctggg tctcttctg 
      gtagcagct gcagagttc ggcggtttt gtcttagga 
      ggcgacatc gattatttg atgttgcac gcaagaact 
      aggccatca cgaattaaa caaaaggag agggcagct 
      gatgaaagc attaaagca aatatggaa cagtataat 
      ttacaacat aaatattta gatgtgctc ataaacgat 
      ttcgcttca tctttttgg agagatgat gttgagttc 
      gattcaata ataaccgat cccccctat gggatccga 
      gaagcaaca gaacgagtg ggcacaagt aaagaaaac 
      ttcaccgtg agcgaaaaa cacctaccc acacacatt 
      ccagctaaa attgaatat ggaatttct agcatttac 
      aacgacttg ttgaaattt gccgcaaaa catttgagg 
      ctgggtggg aggcttgtt tgctggttt cctgtcttt 
      agagaagac tattcggaa gcggtgaac aaatcttga
    mRNA sequence
    • >TC000838 RA
      cactttttc tcactcact acttaaccc aaaaaacgt 
      gtttttgca tttattttt tactcgaaa cgacactat 
      taagttatt caagatgaa taaaccgtg gaaaaaata 
      cctcctgtg gtttgcaca ggaacatat cgaatttcg 
      cttcgaaga gattgattc gttactttc catgtttca 
      aattaaaat gagacactt caaagaacc agataatac 
      cgcgcctta ttggatcgt tgaatttga gtcagaaag 
      tgatttgaa acgtttagt tagcagatc tgtctcgat 
      tagatcatg tatggaatt gtgggcaca tgcgaaaga 
      tatccagag tttacactg tcagttaca gaatttccc 
      aaaacagtt aatggagcc ttactttca accccattt 
      gtctttcaa aattgaagt ggaaacttt ttgtcgcca 
      cttcagcca aaaggaaaa aattaataa gcttgaaag 
      ttttagtta tctgcctgt ccaaggccc tgttaaatt 
      aaaagatcc agatgtttg tttacatta cattgaata 
      ttacggaac aagggctaa tgacccacc tgaaaaccc 
      ttacgatgt gttttttgg cagatggat agctaatgg 
      tttgagaca attaatcag gaaattgtc gttaaaaac 
      tcggaaatt tattggaaa cactagtat ggatccaca 
      gttgtcttt attgatggc taatcaagc ccaagtcaa 
      ggatgggga cattgtctt agacccctt tgtaggctc 
      tgggtctct tctggtagc agctgcaga gttcggcgg 
      ttttgtctt aggaggcga catcgatta tttgatgtt 
      gcacgcaag aactaggcc atcacgaat taaacaaaa 
      ggagagggc agctgatga aagcattaa agcaaatat 
      ggaacagta taatttaca acataaata tttagatgt 
      gctcataaa cgatttcgc ttcatcttt ttggagaga 
      tgatgttga gttcgattc aataataac cgatccccc 
      ctatgggat ccgagaagc aacagaacg agtgggcac 
      aagtaaaga aaacttcac cgtgagcga aaaacacct 
      acccacaca cattccagc taaaattga atatggaat 
      ttctagcat ttacaacga cttgttgaa atttgccgc 
      aaaacattt gaggctggg tgggaggct tgtttgctg 
      gtttcctgt ctttagaga agactattc ggaagcggt 
      gaacaaatc ttgactaaa ataacgtca cgaagactt 
      ttaacgttt gaaaaagtt cgcaatatt aacccaacg 
      gacgattcc gatcagaat aaaattgtg gactttaga 
      gaaaaatat tatgtcgct cgggaggaa acaaggaaa 
      gaaaagcga atgcgggaa gccaggatc cggaaagag 
      aactggatc caatca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY