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Gene information
General information
TC000825 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6287752..6292038
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000825
    iBeetle screen iB_08992

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000825 RA
      MSIQDKVLM RKIKKREKQ KLKLVQLKE KEKNKVVEE 
      EQDEEGFVP QERKGAKRG QNDDTGPAR KKKKKNRKK 
      KDLDLEQSH ESDKESEKE DDEENNDEE EPQEAQTEG 
      DDEQNKLPG AETTLEILS NCTFDSLKN KVCENTLKA 
      IADMGFTTL TEIQARSIP PLLEGRDLV GAAKTGSGK 
      TLAFLIPAV ELIYKLKFM PRNGTGVII ISPTRELSM 
      QTFGVLKEL MKYHHHTYG LVMGGTSRQ TEAQKLSKG 
      INILVATPG RLLDHMQNT PDFLFKNLQ CLVIDEADR 
      ILDIGFEEE MKQIINLLP KRRQTMLFS ATQTKKTEA 
      LTSLALKKE PIYVGVDDA KSEATVTGL EQGYVVCPS 
      EKRLLVLFT FLKKNRKKK VMVFFSSCM SVKFHHELF 
      NYIDLPVMC IHGKQKQAK RTTTFFQFC NAESGILLC 
      TDVAARGLD IPAVDWIVQ YDPPDDPKE YIHRVGRTA 
      RGEGSSGHA LLILRPEEL GFLRYLKQA KVPLNEFEF 
      SWNKIADIQ LQLENLIGK NYFLNMSAK EAFKAYVRA 
      YDSHHLKTI FDISTLDLA KVGLSFGFK VPPAVDLKV 
      TVKKSERPS KRKGGGGFG YYKNLNAMP SKDYKKNVV 
      YRQPKRKGG SDKRQFSR
    CDS sequence
    • >TC000825 RA
      atgtctatt caggataag gtgctaatg agaaaaatt 
      aaaaaacga gaaaaacag aaattaaaa cttgtgcaa 
      ctgaaagag aaagagaaa aataaggtg gttgaggaa 
      gaacaggat gaggagggt tttgttccc caggagcga 
      aagggggcc aaaagggga caaaacgat gatacaggg 
      cctgctcgg aaaaaaaag aagaagaat aggaaaaag 
      aaagatcta gatttagaa cagagccat gaaagtgac 
      aaagaaagc gaaaaagaa gacgatgag gaaaataac 
      gacgaggaa gaaccccaa gaggcacaa accgaagga 
      gatgatgaa caaaataaa cttcctggt gccgaaacc 
      acgttggaa attctaagt aactgcacg tttgattct 
      cttaagaac aaagtttgt gaaaacacc cttaaagct 
      attgctgat atgggcttc acaacactt actgaaatt 
      caagcacga tcaattcct ccattactg gaaggccgc 
      gatttagtc ggggctgct aaaacagga tctggaaaa 
      acgttggct tttcttata ccagcagtc gagttgatt 
      tataaactg aaatttatg ccaagaaat ggtactggt 
      gttattatt atttcacca acaagagaa ttgtcaatg 
      caaacgttc ggggtttta aaagagttg atgaaatat 
      caccaccat acttatggc ttggtcatg ggtgggact 
      agtaggcaa acagaggct cagaaattg tccaaaggg 
      attaatata ctagtggct acacctgga cgtcttttg 
      gatcacatg caaaacact cctgatttt ctttttaag 
      aatttacag tgtcttgtg atcgacgaa gctgatcga 
      attttagat attggtttt gaggaggaa atgaaacag 
      attattaac cttctgcca aaacggaga caaacaatg 
      ttgttcagt gccacacaa acgaagaaa actgaagcc 
      ttgacgtct ctagcgttg aaaaaagag ccgatttat 
      gtcggtgtg gacgacgct aaaagcgag gccactgtt 
      acaggactt gagcaaggc tacgttgtt tgtccttct 
      gaaaaaaga cttttggtc ttgttcact tttctgaag 
      aagaatcgc aagaagaag gtgatggtg tttttcagc 
      tcttgcatg tctgtgaaa ttccatcac gagttgttt 
      aactacatt gacttgcca gtgatgtgc attcatggg 
      aagcagaaa caggcgaag cggacgacc acatttttc 
      cagttctgt aacgcagaa tctggaatt ttgctctgt 
      actgacgtc gcagcgaga ggtttggac attccagcc 
      gttgactgg atcgtgcaa tatgaccct ccagatgac 
      cctaaagag tacattcac agagttggc agaactgca 
      agaggtgaa ggaagcagt ggtcatgct ttgctaatt 
      ttaagacct gaggaactt ggtttcctt aggtatcta 
      aaacaggct aaagtaccg ttgaatgag tttgagttt 
      agttggaat aaaattgcc gatatccag ttacagttg 
      gagaattta attggcaaa aattatttt ttgaacatg 
      tcggcgaaa gaagcgttt aaggcttat gtgagagcg 
      tacgactcg catcatttg aagaccatt tttgatatc 
      agtacgctt gatttggcg aaggttggg ttgtcgttt 
      gggtttaaa gtaccacca gctgtggat ttaaaagtt 
      actgttaaa aaatctgaa cgcccttct aagagaaag 
      ggtgggggc ggctttggt tactacaag aatttgaat 
      gcaatgccg agtaaagat tacaagaag aatgttgtc 
      tacagacaa cctaaacgc aagggcggc tcagataaa 
      cgccagttt tcaagatga
    mRNA sequence
    • >TC000825 RA
      aacctcaaa ttctggaaa gttcaagct cctcacacg 
      tgttttatt ttttgtgtt aaaatgtct attcaggat 
      aaggtgcta atgagaaaa attaaaaaa cgagaaaaa 
      cagaaatta aaacttgtg caactgaaa gagaaagag 
      aaaaataag gtggttgag gaagaacag gatgaggag 
      ggttttgtt ccccaggag cgaaagggg gccaaaagg 
      ggacaaaac gatgataca gggcctgct cggaaaaaa 
      aagaagaag aataggaaa aagaaagat ctagattta 
      gaacagagc catgaaagt gacaaagaa agcgaaaaa 
      gaagacgat gaggaaaat aacgacgag gaagaaccc 
      caagaggca caaaccgaa ggagatgat gaacaaaat 
      aaacttcct ggtgccgaa accacgttg gaaattcta 
      agtaactgc acgtttgat tctcttaag aacaaagtt 
      tgtgaaaac acccttaaa gctattgct gatatgggc 
      ttcacaaca cttactgaa attcaagca cgatcaatt 
      cctccatta ctggaaggc cgcgattta gtcggggct 
      gctaaaaca ggatctgga aaaacgttg gcttttctt 
      ataccagca gtcgagttg atttataaa ctgaaattt 
      atgccaaga aatggtact ggtgttatt attatttca 
      ccaacaaga gaattgtca atgcaaacg ttcggggtt 
      ttaaaagag ttgatgaaa tatcaccac catacttat 
      ggcttggtc atgggtggg actagtagg caaacagag 
      gctcagaaa ttgtccaaa gggattaat atactagtg 
      gctacacct ggacgtctt ttggatcac atgcaaaac 
      actcctgat tttcttttt aagaattta cagtgtctt 
      gtgatcgac gaagctgat cgaatttta gatattggt 
      tttgaggag gaaatgaaa cagattatt aaccttctg 
      ccaaaacgg agacaaaca atgttgttc agtgccaca 
      caaacgaag aaaactgaa gccttgacg tctctagcg 
      ttgaaaaaa gagccgatt tatgtcggt gtggacgac 
      gctaaaagc gaggccact gttacagga cttgagcaa 
      ggctacgtt gtttgtcct tctgaaaaa agacttttg 
      gtcttgttc acttttctg aagaagaat cgcaagaag 
      aaggtgatg gtgtttttc agctcttgc atgtctgtg 
      aaattccat cacgagttg tttaactac attgacttg 
      ccagtgatg tgcattcat gggaagcag aaacaggcg 
      aagcggacg accacattt ttccagttc tgtaacgca 
      gaatctgga attttgctc tgtactgac gtcgcagcg 
      agaggtttg gacattcca gccgttgac tggatcgtg 
      caatatgac cctccagat gaccctaaa gagtacatt 
      cacagagtt ggcagaact gcaagaggt gaaggaagc 
      agtggtcat gctttgcta attttaaga cctgaggaa 
      cttggtttc cttaggtat ctaaaacag gctaaagta 
      ccgttgaat gagtttgag tttagttgg aataaaatt 
      gccgatatc cagttacag ttggagaat ttaattggc 
      aaaaattat tttttgaac atgtcggcg aaagaagcg 
      tttaaggct tatgtgaga gcgtacgac tcgcatcat 
      ttgaagacc atttttgat atcagtacg cttgatttg 
      gcgaaggtt gggttgtcg tttgggttt aaagtacca 
      ccagctgtg gatttaaaa gttactgtt aaaaaatct 
      gaacgccct tctaagaga aagggtggg ggcggcttt 
      ggttactac aagaatttg aatgcaatg ccgagtaaa 
      gattacaag aagaatgtt gtctacaga caacctaaa 
      cgcaagggc ggctcagat aaacgccag ttttcaaga 
      tgagaaaat attttaagt gttttagat taatatcat 
      atattttac attatggat tatgataat aaatacttt 
      ggactctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY