| General information | |||
| TC000825 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 6287752..6292038 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000825 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08992 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000825 RA
MSIQDKVLM RKIKKREKQ KLKLVQLKE KEKNKVVEE EQDEEGFVP QERKGAKRG QNDDTGPAR KKKKKNRKK KDLDLEQSH ESDKESEKE DDEENNDEE EPQEAQTEG DDEQNKLPG AETTLEILS NCTFDSLKN KVCENTLKA IADMGFTTL TEIQARSIP PLLEGRDLV GAAKTGSGK TLAFLIPAV ELIYKLKFM PRNGTGVII ISPTRELSM QTFGVLKEL MKYHHHTYG LVMGGTSRQ TEAQKLSKG INILVATPG RLLDHMQNT PDFLFKNLQ CLVIDEADR ILDIGFEEE MKQIINLLP KRRQTMLFS ATQTKKTEA LTSLALKKE PIYVGVDDA KSEATVTGL EQGYVVCPS EKRLLVLFT FLKKNRKKK VMVFFSSCM SVKFHHELF NYIDLPVMC IHGKQKQAK RTTTFFQFC NAESGILLC TDVAARGLD IPAVDWIVQ YDPPDDPKE YIHRVGRTA RGEGSSGHA LLILRPEEL GFLRYLKQA KVPLNEFEF SWNKIADIQ LQLENLIGK NYFLNMSAK EAFKAYVRA YDSHHLKTI FDISTLDLA KVGLSFGFK VPPAVDLKV TVKKSERPS KRKGGGGFG YYKNLNAMP SKDYKKNVV YRQPKRKGG SDKRQFSR
- >TC000825 RA
atgtctatt caggataag gtgctaatg agaaaaatt aaaaaacga gaaaaacag aaattaaaa cttgtgcaa ctgaaagag aaagagaaa aataaggtg gttgaggaa gaacaggat gaggagggt tttgttccc caggagcga aagggggcc aaaagggga caaaacgat gatacaggg cctgctcgg aaaaaaaag aagaagaat aggaaaaag aaagatcta gatttagaa cagagccat gaaagtgac aaagaaagc gaaaaagaa gacgatgag gaaaataac gacgaggaa gaaccccaa gaggcacaa accgaagga gatgatgaa caaaataaa cttcctggt gccgaaacc acgttggaa attctaagt aactgcacg tttgattct cttaagaac aaagtttgt gaaaacacc cttaaagct attgctgat atgggcttc acaacactt actgaaatt caagcacga tcaattcct ccattactg gaaggccgc gatttagtc ggggctgct aaaacagga tctggaaaa acgttggct tttcttata ccagcagtc gagttgatt tataaactg aaatttatg ccaagaaat ggtactggt gttattatt atttcacca acaagagaa ttgtcaatg caaacgttc ggggtttta aaagagttg atgaaatat caccaccat acttatggc ttggtcatg ggtgggact agtaggcaa acagaggct cagaaattg tccaaaggg attaatata ctagtggct acacctgga cgtcttttg gatcacatg caaaacact cctgatttt ctttttaag aatttacag tgtcttgtg atcgacgaa gctgatcga attttagat attggtttt gaggaggaa atgaaacag attattaac cttctgcca aaacggaga caaacaatg ttgttcagt gccacacaa acgaagaaa actgaagcc ttgacgtct ctagcgttg aaaaaagag ccgatttat gtcggtgtg gacgacgct aaaagcgag gccactgtt acaggactt gagcaaggc tacgttgtt tgtccttct gaaaaaaga cttttggtc ttgttcact tttctgaag aagaatcgc aagaagaag gtgatggtg tttttcagc tcttgcatg tctgtgaaa ttccatcac gagttgttt aactacatt gacttgcca gtgatgtgc attcatggg aagcagaaa caggcgaag cggacgacc acatttttc cagttctgt aacgcagaa tctggaatt ttgctctgt actgacgtc gcagcgaga ggtttggac attccagcc gttgactgg atcgtgcaa tatgaccct ccagatgac cctaaagag tacattcac agagttggc agaactgca agaggtgaa ggaagcagt ggtcatgct ttgctaatt ttaagacct gaggaactt ggtttcctt aggtatcta aaacaggct aaagtaccg ttgaatgag tttgagttt agttggaat aaaattgcc gatatccag ttacagttg gagaattta attggcaaa aattatttt ttgaacatg tcggcgaaa gaagcgttt aaggcttat gtgagagcg tacgactcg catcatttg aagaccatt tttgatatc agtacgctt gatttggcg aaggttggg ttgtcgttt gggtttaaa gtaccacca gctgtggat ttaaaagtt actgttaaa aaatctgaa cgcccttct aagagaaag ggtgggggc ggctttggt tactacaag aatttgaat gcaatgccg agtaaagat tacaagaag aatgttgtc tacagacaa cctaaacgc aagggcggc tcagataaa cgccagttt tcaagatga
- >TC000825 RA
aacctcaaa ttctggaaa gttcaagct cctcacacg tgttttatt ttttgtgtt aaaatgtct attcaggat aaggtgcta atgagaaaa attaaaaaa cgagaaaaa cagaaatta aaacttgtg caactgaaa gagaaagag aaaaataag gtggttgag gaagaacag gatgaggag ggttttgtt ccccaggag cgaaagggg gccaaaagg ggacaaaac gatgataca gggcctgct cggaaaaaa aagaagaag aataggaaa aagaaagat ctagattta gaacagagc catgaaagt gacaaagaa agcgaaaaa gaagacgat gaggaaaat aacgacgag gaagaaccc caagaggca caaaccgaa ggagatgat gaacaaaat aaacttcct ggtgccgaa accacgttg gaaattcta agtaactgc acgtttgat tctcttaag aacaaagtt tgtgaaaac acccttaaa gctattgct gatatgggc ttcacaaca cttactgaa attcaagca cgatcaatt cctccatta ctggaaggc cgcgattta gtcggggct gctaaaaca ggatctgga aaaacgttg gcttttctt ataccagca gtcgagttg atttataaa ctgaaattt atgccaaga aatggtact ggtgttatt attatttca ccaacaaga gaattgtca atgcaaacg ttcggggtt ttaaaagag ttgatgaaa tatcaccac catacttat ggcttggtc atgggtggg actagtagg caaacagag gctcagaaa ttgtccaaa gggattaat atactagtg gctacacct ggacgtctt ttggatcac atgcaaaac actcctgat tttcttttt aagaattta cagtgtctt gtgatcgac gaagctgat cgaatttta gatattggt tttgaggag gaaatgaaa cagattatt aaccttctg ccaaaacgg agacaaaca atgttgttc agtgccaca caaacgaag aaaactgaa gccttgacg tctctagcg ttgaaaaaa gagccgatt tatgtcggt gtggacgac gctaaaagc gaggccact gttacagga cttgagcaa ggctacgtt gtttgtcct tctgaaaaa agacttttg gtcttgttc acttttctg aagaagaat cgcaagaag aaggtgatg gtgtttttc agctcttgc atgtctgtg aaattccat cacgagttg tttaactac attgacttg ccagtgatg tgcattcat gggaagcag aaacaggcg aagcggacg accacattt ttccagttc tgtaacgca gaatctgga attttgctc tgtactgac gtcgcagcg agaggtttg gacattcca gccgttgac tggatcgtg caatatgac cctccagat gaccctaaa gagtacatt cacagagtt ggcagaact gcaagaggt gaaggaagc agtggtcat gctttgcta attttaaga cctgaggaa cttggtttc cttaggtat ctaaaacag gctaaagta ccgttgaat gagtttgag tttagttgg aataaaatt gccgatatc cagttacag ttggagaat ttaattggc aaaaattat tttttgaac atgtcggcg aaagaagcg tttaaggct tatgtgaga gcgtacgac tcgcatcat ttgaagacc atttttgat atcagtacg cttgatttg gcgaaggtt gggttgtcg tttgggttt aaagtacca ccagctgtg gatttaaaa gttactgtt aaaaaatct gaacgccct tctaagaga aagggtggg ggcggcttt ggttactac aagaatttg aatgcaatg ccgagtaaa gattacaag aagaatgtt gtctacaga caacctaaa cgcaagggc ggctcagat aaacgccag ttttcaaga tgagaaaat attttaagt gttttagat taatatcat atattttac attatggat tatgataat aaatacttt ggactctt
