Status
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Gene information
General information
TC032046
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000757
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5878324..5880457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032046
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08989

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032046 RA
      MLAKIFLLL VFKLSYILC SNVLVELPN GIILGREDK 
      SYTDKPYFI FQKIPYAAP PVGNLRFKA PKLPKDWEG 
      VLNCTYLDK ICYQDTTNL PEESEDCLY INVYTPELK 
      NASIPVLLY IYGGGFVEG HAMQYRRGP EYLIDHDVV 
      IVTFNYRLG ALGFLSTGD AIIPGNNGL KDQQLAIKW 
      VHDNIHLFG GDPKRVTLV GESAGGASV SHHILNAKS 
      EGLFRGAIM ESGTAINPW SLQRNPRQK AFAYGALFN 
      SSFNEIDDS EALLDFLLN LPAADLDKV SPLQSGWAP 
      VIEVEHEDA FITKKMYGL VQSGNFVKV PILMGINSE 
      ESLYEAGDL DSLKSECEM YDSNVDRLV PEDVSVVLN 
      NETIVKFGQ AIKQIYCDH ENFIDNMSA AVRFSSDNS 
      FTRAIIKCA EMKSQFTDV YYYQFSYDG IMGNVTISI 
      EGAEHVAHA EELRYVWRT TRDGYDNSD LSKFPPEDV 
      LTQHRMLTL WTNFVKNLN PTPDTSDLL QNVTWPTVT 
      SGGVGDFLY LDIGENLEV RNHPKADTY HRWI
    CDS sequence
    • >TC032046 RA
      atgttagcg aaaattttc ctattactc gtcttcaaa 
      ctatcgtat attctgtgt tccaatgtt ttggtggaa 
      cttcctaac ggcataatc cttggacgt gaagataaa 
      tcatacact gataaaccc tattttatt ttccaaaaa 
      attccatat gctgctcca cctgttgga aatcttcgg 
      tttaaagca ccaaaactc cctaaagac tgggaagga 
      gttctaaat tgtacatat ctcgataaa atctgttat 
      caagacacg actaacttg ccagaggag tccgaagat 
      tgtctctac attaatgtt tacacacca gaactgaaa 
      aatgcctct atacctgtt ctgttgtat atttacggt 
      ggtggattt gttgagggt catgcaatg cagtaccga 
      cgaggccct gaatattta attgatcat gacgttgtg 
      attgttaca tttaattac agactggga gctttaggt 
      tttttatcg accggagat gcaataata cccggaaat 
      aatggctta aaagaccaa caactggcc ataaaatgg 
      gtccatgat aatatccat ttgttcgga ggggatccc 
      aaaagagtg actttagtg ggcgaaagc gcaggtggt 
      gcttcagtc agtcatcac attttgaac gcaaagtcg 
      gaaggctta ttccgagga gcgattatg gaaagtggc 
      actgcgatt aatccatgg tccctccaa agaaacccg 
      agacaaaaa gctttcgcc tatggtgcg ctttttaat 
      tcgagtttt aacgaaatt gatgattct gaagcactt 
      ctggacttt ctactcaat ttaccagct gctgattta 
      gataaagtc tcgccctta caatcagga tgggcacca 
      gttattgaa gttgagcat gaggatgct tttattacg 
      aaaaaaatg tacggtctt gtgcagtca gggaatttt 
      gtcaaagtg ccgattttg atgggaatt aattccgaa 
      gaatcccta tatgaggct ggagactta gattcgctt 
      aaatcggaa tgtgaaatg tacgacagc aacgtggat 
      cgtttagta cctgaagat gtgagtgta gttctcaac 
      aatgagact attgtcaag tttgggcaa gccatcaaa 
      caaatttat tgtgaccac gaaaatttt atcgacaat 
      atgagtgca gcagtacgg ttttctagt gataatagc 
      ttcacacga gccattata aaatgtgcc gaaatgaaa 
      tcacaattc actgacgtt tattattat cagttctct 
      tatgacggc ataatgggg aacgttact atcagtatt 
      gaaggtgct gaacacgtt gcacatgct gaagagctt 
      cgctatgtg tggcggacc actcgggat ggatatgat 
      aattctgac ttgagtaaa tttccccca gaggatgtt 
      ctgacccaa cacagaatg ctaacactt tggaccaat 
      tttgttaaa aacttaaac cccacaccg gacacaagt 
      gatctttta caaaatgtt acttggcct acggtgaca 
      tcgggaggt gtaggcgat tttttatat ttagatatt 
      ggagagaat ttggaagtt aggaatcat cccaaagca 
      gatacctat cacagatgg atttaa
    mRNA sequence
    • >TC032046 RA
      agtgcgtgc atctaacat gttagcgaa aattttcct 
      attactcgt cttcaaact atcgtatat tctgtgttc 
      caatgtttt ggtggaact tcctaacgg cataatcct 
      tggacgtga agataaatc atacactga taaacccta 
      ttttatttt ccaaaaaat tccatatgc tgctccacc 
      tgttggaaa tcttcggtt taaagcacc aaaactccc 
      taaagactg ggaaggagt tctaaattg tacatatct 
      cgataaaat ctgttatca agacacgac taacttgcc 
      agaggagtc cgaagattg tctctacat taatgttta 
      cacaccaga actgaaaaa tgcctctat acctgttct 
      gttgtatat ttacggtgg tggatttgt tgagggtca 
      tgcaatgca gtaccgacg aggccctga atatttaat 
      tgatcatga cgttgtgat tgttacatt taattacag 
      actgggagc tttaggttt tttatcgac cggagatgc 
      aataatacc cggaaataa tggcttaaa agaccaaca 
      actggccat aaaatgggt ccatgataa tatccattt 
      gttcggagg ggatcccaa aagagtgac tttagtggg 
      cgaaagcgc aggtggtgc ttcagtcag tcatcacat 
      tttgaacgc aaagtcgga aggcttatt ccgaggagc 
      gattatgga aagtggcac tgcgattaa tccatggtc 
      cctccaaag aaacccgag acaaaaagc tttcgccta 
      tggtgcgct ttttaattc gagttttaa cgaaattga 
      tgattctga agcacttct ggactttct actcaattt 
      accagctgc tgatttaga taaagtctc gcccttaca 
      atcaggatg ggcaccagt tattgaagt tgagcatga 
      ggatgcttt tattacgaa aaaaatgta cggtcttgt 
      gcagtcagg gaattttgt caaagtgcc gattttgat 
      gggaattaa ttccgaaga atccctata tgaggctgg 
      agacttaga ttcgcttaa atcggaatg tgaaatgta 
      cgacagcaa cgtggatcg tttagtacc tgaagatgt 
      gagtgtagt tctcaacaa tgagactat tgtcaagtt 
      tgggcaagc catcaaaca aatttattg tgaccacga 
      aaattttat cgacaatat gagtgcagc agtacggtt 
      ttctagtga taatagctt cacacgagc cattataaa 
      atgtgccga aatgaaatc acaattcac tgacgttta 
      ttattatca gttctctta tgacggcat aatggggaa 
      cgttactat cagtattga aggtgctga acacgttgc 
      acatgctga agagcttcg ctatgtgtg gcggaccac 
      tcgggatgg atatgataa ttctgactt gagtaaatt 
      tcccccaga ggatgttct gacccaaca cagaatgct 
      aacactttg gaccaattt tgttaaaaa cttaaaccc 
      cacaccgga cacaagtga tcttttaca aaatgttac 
      ttggcctac ggtgacatc gggaggtgt aggcgattt 
      tttatattt agatattgg agagaattt ggaagttag 
      gaatcatcc caaagcaga tacctatca cagatggat 
      ttaacttta cgctatgtt gaactataa tgattttga 
      tacattttg aaatgtaat aaaattgat ttgtcttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY