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Gene information
General information
TC034550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5844381..5846877
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034550
    iBeetle screen iB_08988

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    21

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034550 RA
      MLKLCILCV LCVTCCAVP NYNSKLFTQ WRDFPSKIF 
      LNTSLLRPE CQQAYENFL DGLNKHDLN ALKMLDATA 
      KIPSGILNG NIVQYGDFN ECMGVKTAQ YCLAEIDFG 
      ALWNESYSE YRDLVQSFN FFKDTFDAP AHRVPGFTM 
      ARWGFCIPQ ECSAEDLEK SLSANFGVL AQIRPGMCQ 
      KATKESKDL TRGDYIARL FFICVVLLV VSSTLVDYY 
      RDGKGVAHT NGIFMKILQ CFSLRENVS KFFEVKEND 
      RSEIAGLHG IRFLNALAL LMSHKSVSL FFNPYMNRT 
      AAVDRVSRP WTIIAKNAI VYTDVFMLI SGLLNANAL 
      LTDLHNGRA MKFKEKLIN RIFSIVY
    CDS sequence
    • >TC034550 RA
      atgctcaaa ctgtgtatt ctgtgcgtc ctttgtgtc 
      acttgttgt gctgtgcca aattataat tcaaaatta 
      ttcacacaa tggcgggat tttccctcg aaaatattt 
      ctcaacact agtttacta cgtccagag tgccaacaa 
      gcctacgag aattttctg gacggttta aataagcat 
      gacttaaac gctttgaaa atgttggac gccacagcc 
      aaaataccg tccggaatt ttgaacggc aatatcgta 
      caatatggc gattttaac gaatgtatg ggggttaaa 
      acggcccaa tattgtctc gctgaaatt gattttgga 
      gcactgtgg aacgaatcc tactccgag tacagggat 
      ttagtgcag tcttttaat ttctttaag gacacgttt 
      gatgctccc gctcataga gttcctggt ttcactatg 
      gcccgttgg ggattttgt ataccccag gaatgttca 
      gcagaagat ttagaaaaa tcgctaagt gcaaatttt 
      ggggttttg gcccaaatt aggccagga atgtgccaa 
      aaagcaaca aaagagagt aaagattta accagaggg 
      gactacatt gccagatta ttttttatt tgtgtcgtt 
      ctattagtg gtttcgtca actttggtc gattattat 
      agagacggt aaaggcgtt gcgcacaca aatggaata 
      ttcatgaaa atactgcag tgtttttca ttgcgcgaa 
      aacgtgtca aaatttttc gaagtgaaa gaaaacgat 
      cgcagcgaa atagctggc ttacacggc attcgtttc 
      ctcaatgct ttggcttta ctcatgtcc cataaaagc 
      gtttcgcta tttttcaac ccttatatg aaccgaaca 
      gctgcagtt gatagagtg agccgacct tggacaatt 
      attgcaaaa aacgcgatt gtttataca gatgtcttc 
      atgcttata agtggatta ctaaacgcg aacgcttta 
      cttaccgac ttacacaat ggccgtgcg atgaaattc 
      aaggaaaaa ttaatcaac aggatattc agtattgtt 
      tattaa
    mRNA sequence
    • >TC034550 RA
      ttcatttta accaaaatg ctcaaactg tgtattctg 
      tgcgtcctt tgtgtcact tgttgtgct gtgccaaat 
      tataattca aaattattc acacaatgg cgggatttt 
      ccctcgaaa atatttctc aacactagt ttactacgt 
      ccagagtgc caacaagcc tacgagaat tttctggac 
      ggtttaaat aagcatgac ttaaacgct ttgaaaatg 
      ttggacgcc acagccaaa ataccgtcc ggaattttg 
      aacggcaat atcgtacaa tatggcgat tttaacgaa 
      tgtatgggg gttaaaacg gcccaatat tgtctcgct 
      gaaattgat tttggagca ctgtggaac gaatcctac 
      tccgagtac agggattta gtgcagtct tttaatttc 
      tttaaggac acgtttgat gctcccgct catagagtt 
      cctggtttc actatggcc cgttgggga ttttgtata 
      ccccaggaa tgttcagca gaagattta gaaaaatcg 
      ctaagtgca aattttggg gttttggcc caaattagg 
      ccaggaatg tgccaaaaa gcaacaaaa gagagtaaa 
      gatttaacc agaggggac tacattgcc agattattt 
      tttatttgt gtcgttcta ttagtggtt tcgtcaact 
      ttggtcgat tattataga gacggtaaa ggcgttgcg 
      cacacaaat ggaatattc atgaaaata ctgcagtgt 
      ttttcattg cgcgaaaac gtgtcaaaa tttttcgaa 
      gtgaaagaa aacgatcgc agcgaaata gctggctta 
      cacggcatt cgtttcctc aatgctttg gctttactc 
      atgtcccat aaaagcgtt tcgctattt ttcaaccct 
      tatatgaac cgaacagct gcagttgat agagtgagc 
      cgaccttgg acaattatt gcaaaaaac gcgattgtt 
      tatacagat gtcttcatg cttataagt ggattacta 
      aacgcgaac gctttactt accgactta cacaatggc 
      cgtgcgatg aaattcaag gaaaaatta atcaacagg 
      atattcagt attgtttat taaaccata tttacatct 
      aaatccata aacaatgac ttgacactt aaataaacg 
      ttagtggtt cat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY