Status
Please wait ...
Gene information
General information
TC032041
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000761
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5837910..5839567
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032041
    iBeetle screen iB_08987

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032041 RA
      MGSGPLWPV VVDHHSTLC KQHMWKNIF FIHNYFGFE 
      NMCLTHTHQ LGIDMQLFL VTPVFVYLI WTFKRIGLF 
      FMGVVALLS TILRFWVSY EHELTSVVY FGISVSKMF 
      SVASLSYIL PTHRATIYL IGVYLAYLL KCSKPNTSF 
      TKWQVAFLW CFFLPMFLG AWLGPYHMV TKDYVYDKL 
      EASYVLALY PIFWGSSVA FGIYAINRD IAGWLGTLF 
      NWKYFQIST KLAYALYLV QFPIFFYNV GLTKHVDEY 
      RTYLQLEVV ETIVIILCS FLLVLLIEM PFQNIKKVL 
      FDQPKRRDQ HQHMRLKDF KSG
    CDS sequence
    • >TC032041 RA
      atgggctcc gggccgtta tggcctgtc gtcgtcgat 
      caccattca acactatgc aagcagcac atgtggaag 
      aatattttt ttcatccac aattacttc ggttttgaa 
      aatatgtgt ttgactcac acgcaccag ctaggtatt 
      gacatgcag ctgttttta gttacacca gtttttgtt 
      tatttgatc tggacattt aaacgaatt ggattattc 
      tttatggga gttgtggcc ctactttca acaatactt 
      agattttgg gtttcgtac gaacatgaa ttgacgagc 
      gtcgtttac ttcggcatc tcagtttca aaaatgttt 
      tcagtggct agtttatca tacatttta ccaactcat 
      cgagcaact atttattta ataggtgtt tacctggct 
      tatttatta aaatgttct aaaccaaac acttctttc 
      actaagtgg caagttgca tttttgtgg tgctttttc 
      ctaccaatg tttctcggt gcttggcta ggaccgtac 
      catatggtc acaaaagac tatgtttat gataaattg 
      gaagcctcg tatgtgctt gctttatat ccgattttt 
      tggggctcc agtgtagca tttggaatt tatgccata 
      aatagagat attgcaggc tggttggga acgttgttt 
      aattggaaa tattttcaa atttcaaca aaactggct 
      tacgccttg tatttggtg caatttcca atttttttc 
      tataatgtg gggttgaca aaacatgtt gacgaatac 
      cgcacatat ttacaactt gaggttgtt gaaaccata 
      gttatcata ttgtgttcc tttttgctt gttcttttg 
      attgaaatg ccgtttcaa aacattaaa aaagtatta 
      tttgatcaa ccgaaaaga cgtgaccaa caccaacat 
      atgcgcttg aaggacttt aaaagtggt taa
    mRNA sequence
    • >TC032041 RA
      gttctttgt gatcaccca cgcccaaca cccagagcc 
      ggctggcac caacttgtg ttacttaga attcttcca 
      aacgtaatt gccctaatt ttattttgt acgtacata 
      ttacccttg atgggctcc gggccgtta tggcctgtc 
      gtcgtcgat caccattca acactatgc aagcagcac 
      atgtggaag aatattttt ttcatccac aattacttc 
      ggttttgaa aatatgtgt ttgactcac acgcaccag 
      ctaggtatt gacatgcag ctgttttta gttacacca 
      gtttttgtt tatttgatc tggacattt aaacgaatt 
      ggattattc tttatggga gttgtggcc ctactttca 
      acaatactt agattttgg gtttcgtac gaacatgaa 
      ttgacgagc gtcgtttac ttcggcatc tcagtttca 
      aaaatgttt tcagtggct agtttatca tacatttta 
      ccaactcat cgagcaact atttattta ataggtgtt 
      tacctggct tatttatta aaatgttct aaaccaaac 
      acttctttc actaagtgg caagttgca tttttgtgg 
      tgctttttc ctaccaatg tttctcggt gcttggcta 
      ggaccgtac catatggtc acaaaagac tatgtttat 
      gataaattg gaagcctcg tatgtgctt gctttatat 
      ccgattttt tggggctcc agtgtagca tttggaatt 
      tatgccata aatagagat attgcaggc tggttggga 
      acgttgttt aattggaaa tattttcaa atttcaaca 
      aaactggct tacgccttg tatttggtg caatttcca 
      atttttttc tataatgtg gggttgaca aaacatgtt 
      gacgaatac cgcacatat ttacaactt gaggttgtt 
      gaaaccata gttatcata ttgtgttcc tttttgctt 
      gttcttttg attgaaatg ccgtttcaa aacattaaa 
      aaagtatta tttgatcaa ccgaaaaga cgtgaccaa 
      caccaacat atgcgcttg aaggacttt aaaagtggt 
      taactgtta agttttatt tattgattt ttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY